Apaf1 (NM_023979) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207514 representing NM_023979 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCAAAGGCCCGAAATTGTTTGCTTCAACATAAAGAAGCTCTGGAAAAGGACATCAAAACATCCT ACATCATGGATCACATGATCAGTAATGGCGTCTTAACAGTAGTAGAGGAGGAAAAGGTCAAAAGTCAGGC CACTCAATATCAACGAGCAGCTGCTTTAATTAAAATGATACTTAATAAAGACAATTATGCCTACATTTCA TTCTACAACGCTCTACTACACGAGGGCTATAAAGACCTTGCTGGACTTCTGCATAGCGGCCTTCCTCTTG TGTCTTCTTCCAGCGGCAAGGACACAGACGGTGGAAACACTTCATTTGTAAGGACAGTGCTGTGTGAAGG TGGAGTGCCACAGAGGCCAGTCATCTTTGTCACTAGGAAGAAGCTGGTCAGTGCGATTCAGCAGAAGCTC TGGAAACTGAATGGGGAGCCAGGGTGGGTCACCATCTATGGAATGGCAGGCTGTGGCAAGTCTGTATTAG CTGCGGAAGCTGTTCGCGACCATGCCCTCCTAGAAGGTTGTTTTTCAGGGGGCGTACACTGGGTTTCCAT CGGAAAACAAGACAAGTCCGGGCTTCTAATGAAGCTGCAGAATCTTTGCACGCGGTTGGGTCAAGAAGAG AGTTTCTCTCAGAGGCTCCCGCTTAACATTGAGGAGGCCAAAGACCGTCTCCGTGTTCTGATGCTGCGAA AACACCCAAGGTCTCTGTTGATCTTGGATGACGTTTGGGATCCTTGGGTGTTAAAGGCTTTTGACAACCA GTGTCAGATTCTTCTTACCACCAGAGATAAGAGTGTTACCGATTCAGTAATGGGTCCTAAGTATGTTATC CCTGTGGAGAGTGGTCTAGGGAAAGAGAAGGGACTTGAAATCTTGTCACTTTTTGTTAATATGAAGAAAG AAGATCTGCCAGTGGAGGCTCACAGTATTATAAAGGAGTGCAAAGGCTCTCCTCTCGTCGTGTCTTTAGT TGGTGCGCTTTTACGGGATTTTCCCAATCGCTGGGCATACTACCTCAGGCAGCTTCAGAATAAGCAGTTT AAGAGAATAAGGAAATCTTCATCTTATGACTACGAGGCTCTGGATGAAGCCATGTCCATAAGTGTGGAGA TGCTCAGAGAAGACATCAAGGACTACTACACAGACCTCTCCATCCTTCAGAAGGATGTCAAGGTGCCTAC GAAGGTGCTGTGTGTTCTCTGGGACTTGGAAACGGAAGAAGTTGAAGACATCTTGCAGGAGTTCGTTAAT AAGTCTCTCTTATTCTGTAATCGGAATGGGAAGTCATTTTGTTATTATTTACACGATCTTCAAGTAGATT TCCTTACAGAGAAGAATCGAAGTCAGCTCCAGGATCTGCACAGGAAGATGGTCACTCAATTTCAGAGGTA TCACCAGCCGCATACGCTGTCTCCAGGTCAGGAGGACTGCATGTACTGGTACAACTTCCTGGCCTATCAT ATGGCCAGTGCCGGTATGCACAAAGAACTTTGTGCTTTAATGTTTTCCCTGGATTGGATTAAAGCAAAAA CAGAACTTGTCGGCCCTGCGCATCTGATTCATGAGTTCGTGGAATATAGGCATATACTGGATGAAAAGGA TTGTGCAGTCTGTGAGAATTTTCAGGAGTTTTTATCTTTAAACGGACACCTTCTTGGACGACAGCCATTT CCTAACATTGTGCAACTGGGCCTCTGTGAACCAGAGACTTCCGAAGTTTATCAACAAGCAAAGCTGCAGG CCAAGCAGGAGGTGGATACCGGGCGGCTTTACCTGGAATGGATCAACAAAAAAACCATCAAGAATCTGTC CCGCTTAGTCGTTCGCCCCCATACAGACGCCGTGTACCATGCGTGTTTTTCTCAGGATGGTCAGAGAATA GCTTCTTGTGGAGCTGATAAAACCTTGCAGGTGTTTAAAGCCGAGACAGGAGAGAAACTTCTTGACATTA AAGCTCATGAAGATGAGGTGCTTTGCTGTGCGTTCTCCTCAGATGACAGTTACATAGCAACCTGCTCAGT GGATAAGAAAGTTAAGATTTGGGATTCTGGGACTGGGAAGCTAGTGCACACCTACGAAGAACACTCAGAG CAGGTCAATTGCTGCCATTTCACCAATAAGAGCAACCACCTTCTTTTGGCCACCGGGTCAAATGACTCCT TCCTTAAGCTCTGGGATTTGAATCAAAAAGAATGCCGAAATACCATGTTCGGTCACACGAACTCAGTCAC CCACTGCAGGTTCTCACCAGACGATGAGCTCTTGGCGAGTTGCTCAGCCGACGGGACTTTAAAGCTTTGG GATGTGAGATCAGCAAACGAGAAGAAAAGCATTAATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCAC CAGAGGACGTGGAGGTGATCGTGAAATGTTGCTCCTGGTCTGCAGATGGCGACAGAATAATAGTGGCAGC AAAAAACAAAGTCCTCCTTCTTGATATTCATACTAGTGGCCTGCTGACTGAGATCCACACAGGTCATCAC AGCACCATCCAGTACTGTGACTTCTCCCCCTACGACCATTTGGCAGTGATTGCCCTGTCTCAGTACTGTG TGGAGTTGTGGAACATAGACTCCCGGGTAAAGGTGGCTGACTGCAGGGGACATTTAAGTTGGGTCCACGG TGTGATGTTTTCTCCTGATGGCTCCTCGTTCTTGACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAG ACAAGAAAGGTGTGCAAGAACTCTGCCATTGTGTTAAAGCAGGAAATAGATGTCGTGTTTCAAGAAAACG AAATGATGGTCCTTGCAGTTGACAACATAAGAGGCCTACAACTCATTGCTGGAAAAACAGGCCAGATTGA TTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTGCCTAAGTCCACATCTTGAGTACGTTGCATTTGGAGAT GAAGAGGGAGCCATTAAGATTATAGAACTTCCAAACAACAGAGTTTTCAGCTCTGGGATTGGGCACAAGA AAGCTGTACGGCACATCCAGTTCACAGCTGATGGGAAGACACTGATTTCAAGTTCTGAAGACTCTGTGAT TCAGGTGTGGAATTGGCAGACAGAGGAGTATGTATTTCTGCAAGCCCATCAGGAAACAGTAAAGGACTTC AGACTCCTGAGAGATTCACGATTGCTTTCCTGGTCATTCGATGGAACGGTGAAGGTGTGGAATGTCATTA CCGGAAGAATTGAAAGAGACTTTACTTGTCATCAGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGC CACCAAGTTTTCATCTACCTCAGCTGATAAGACCGCCAAGATCTGGAGTTTTGAACTCCCTTCCCCTCTT CATGAGCTGAAGGGCCATAACAGCTGTGTCCGCTGCTCTGCCTTCTCGCTGGATGGCATCCTGTTAGCAA CTGGAGATGACAATGGAGAAATCAGGATATGGAATGTCTCAGATGGCCAGCTTCTTCATTTGTGTGCTCC GATTTCCATAGAAGAAGGAACGGCTACCCACGGAGGCTGGGTGACTGATGTTTGCTTCTCTCCTGACAGA AAAATGCTTGTCTCTGCCGGAGGATACCTTAAGTGGTGGAATGTTGTTACTGGGGAGTCCTCACAGACCT TCTACACCAATGGAACAAACCTCAAGAAAATACATGTGTCCCCTGACTTCAGAACATATGTGACCGTTGA TAATCTTGGTATTTTATATATTTTACAGGTTTTAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_023979
Insert Size	3750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_023979.1, NP_076469.1
RefSeq Size	6111 bp
RefSeq ORF	3750 bp
Locus ID	78963
UniProt ID	Q9EPV5
Gene Summary	protein involved in the initiation of apoptosis [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.