Txnrd2 (NM_022584) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Synonyms	Tr3; Trxr2; Trxrd2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207441 representing NM_022584 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGATTGTGGCGGCGCTGCGCGGATCCAGCGGGCGCTTCCGGCCGCAGACACGGGTTTTAACAC GCGGGACGCGGGGCGCGGCGGGCGCGGCGAGCGCAGCGGGAGGGCAGCAGAACTTTGATCTCTTGGTGAT CGGTGGGGGATCCGGTGGCCTAGCTTGTGCCAAGGAAGCGGCTCAGCTGGGAAGGAAGGTGGCTGTGGCT GACTATGTGGAACCCTCTCCCCGAGGCACCAAATGGGGCCTTGGTGGCACCTGTGTCAACGTGGGCTGCA TACCCAAGAAGCTGATGCATCAGGCCGCACTGCTGGGGGGCATGATCAGAGATGCTCAGCACTACGGCTG GGAGGTGGCCCAGCCTGTCCAGCACAACTGGAAGGCAATGGCCGAAGCCGTGCAAAACCATGTGAAGTCC TTGAACTGGGGTCATCGTGTCCAACTGCAGGACAGGAAAGTCAAGTACTTTAACATCAAAGCCAGCTTTG TCAACGAGCACACAGTTCACGGTGTCGACAAAGCCGGGAAGGTGACTCAGCTTTCAGCCAAGCACATAGT CATCGCTACAGGAGGACGGCCGAAGTACCCCACACAGGTCAAAGGAGCCCTGGAACACGGAATCACAAGT GATGACATCTTCTGGCTGAAGGAGTCCCCTGGGAAAACGTTGGTGGTTGGAGCCAGTTATGTGGCCCTGG AGTGTGCCGGCTTCCTCACTGGTATTGGCCTGGATACCACGGTCATGATGCGCAGCGTGCCCCTCCGAGG CTTTGACCAGCAAATGGCGTCTTTGGTCACAGAGCACATGGAGTCTCATGGCACCCGGTTCCTGAAAGGC TGTGTCCCCTCCCTCATCAGAAAACTCCCGACTAACCAACTGCAGGTCACTTGGGAGGATCTCGCTTCTG GCAAGGAGGACGTGGGCACCTTTGACACTGTCCTGTGGGCCATAGGGCGAGTTCCAGAGACCAGAAATTT GAATCTGGAGAAGGCTGGCGTTAATACCAACCCTAAGAATCAGAAGATCATTGTGGATGCCCAGGAGGCC ACCTCTGTCCCCCACATCTATGCCATTGGAGATGTTGCTGAGGGGCGGCCTGAGCTGACACCCACAGCTA TCAAGGCAGGAAAGCTTCTGGCTCAGCGGCTCTTTGGGAAATCCTCAACCTTAATGAATTACAGCAACGT CCCCACAACTGTCTTTACACCACTGGAGTATGGCTGTGTGGGACTGTCTGAGGAGGAGGCTGTGGCTCTC CACGGCCAGGAGCATATAGAGGTTTACCATGCATATTACAAGCCCCTAGAGTTCACAGTGGCAGATCGGG ATGCATCACAGTGCTACATAAAGATGGTATGCATGAGGGAGCCCCCACAACTGGTACTGGGCCTGCACTT CCTTGGCCCCAACGCTGGAGAAGTCACACAAGGATTTGCTCTTGGGATCCAGTGTGGGGCTTCATACGCA CAGGTGATGCAGACAGTAGGGATCCACCCCACCTGCTCTGAGGAGGTGGTTAAGCTGCACATCTCCAAGC GCTCTGGCCTGGATCCTACTGTGACTGGCTGCTGAGGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022584
Insert Size	1581 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022584.2, NP_072106.1
RefSeq Size	1989 bp
RefSeq ORF	1581 bp
Locus ID	50551
UniProt ID	Q9Z0J5
Gene Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2017]

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