United States
Germany
Japan
United Kingdom
China
Rbbp8 (NM_001134417) Rat Untagged Clone
CAT＃: RN207175
Rbbp8 (untagged ORF) - Rat retinoblastoma binding protein 8 (Rbbp8), (10 ug)
CNY 11,590.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | CtIP; RBBP-8; RGD1308872; RIM; SAE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207175 representing NM_001134417
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATTTCAGGAAGCAGCTGTGGAAGCCCTAATTCCACAGATATATCCAGTGACTTCAAGGAGCTCT GGACAAAACTAAAAGAATATCATGATAAGGAAGTACAAGGTTTACAAATCAAAGTAACCAAACTGAAAAA GGAAAGAATTTTAGATGCACAGAGACTAGAAGAATTCTTCACCAAAAATCAACAGCTGAGAGACCAGCAA AAAGTTCTTCAGGAAACCATTAAAATTTTAGAAGATAGGTTAAGAGCAGGACTGTGTGATCGCTGTGCAG TAACTGAAGAGCATATGCACAAAAAGCAGCAGGAGTTTGAAAACATCCGACAGCAGAACCTTAGGCTTAT CACAGAGCTCATGAATGAAAAGAGCGCTCTTCAGGAAGAAAACAAAAAGCTTTCTGAGCAACTGCAACAG AAAATGGAAAGTGGTCAACAGGATCAAGTAGCTGAGCTTGAATGTGAGGAAAACATTATTCCAGACTCAC CGATAACTTCCTTTTCCTTTTCTGGCATTAACCGCCTACGAAGAAAGGAGAATCTCCATGTCCGATACGT TGAACAAACGCACACTAAATTGGAGCACTCTGCATGTACAAGTGAATTAAGAAAGTTTTCCAAGGGTTCA ACTCCTGCACCAGTTAACTCTGAGGAACATGAAATTCTAGTAGCCGACACTTGTGATCAGAGTCATTCTC CACTGTCTAAAATATGTGGAACAAGCAGTTACCCCGCTGACAAGTTATCTTCTAACTTAGATGCTGTTGT TGCTGAGACGCTTGGACTTGATGGTCAAGAAGAATCTGAACCCCAAGGTCCTGTGAGCCCTCTTGGAAAT GAGCTCTACCACTGTCTCAAAGAAGATCACAAAAAACAGCCTTTTATGGAATCTGCAATAAGGAACGAAG ATAACGTAAGGTTTTCAGACTCTGCTTCAAAGGCCCCTCCCCGAGAACTCACTACTCGGGGGTCATCTCC CGTGTTTGGACCTACCTCTACCGTGAAAACTCATTTGGGTTTGAAAACAAGTTTCTCCCCTTCACTCTTA GACAGTGGGAAAAAAAACCTTCTGAGCACAGCTCCCTTTAGCAGCATTTCTGTTTCTAGATCAGAGAAAG TTAGATCCAAATCAGAGGATAATGCCCTTTTCACACAACAAAGTGCTGGGTCTGAGGTAAAGGTCATCAG CCAGTCATTTCCTAGTAAGCAGATTCTTACAAGTAAAAACGTTAGTGACTCTGTCGACGAGCAGGGTGGT GCTGATCACATGAAAGATGCTGTTAGTGATAAACACCTGGTGCCTTTGAAATCACTGGGAGGCAAAGCGT CCAAAAGGAAGAGGACTGAGGAAGAGGGTGAGCACGCAGTACACTGCCCGCAGACCTGTTTTGATAAGGA AAATGCCCTTCCTTTTCCAATGGAGAATCAGTTCCCTGTGAATGGAGACCATGTGATGGATAAACCTCTG GACCTGTCAGATCGGTTTGCAGCTAATCAGCGTCAAGAGAAGAACCACGGGGATGAAACTTGCAAACACA AACTTAAGCAAGTGACTATTTATGAGGCTCTGAAGCCCGTTCCCAAGGGCTCCTCCTCAGGCCGCAAGGC CTTGAGTGGGGCCTGCACACTAGCCCAAGACTCTGCAGAGACATACTGCTTACAGCAGCGCACCCTCCAG TGCTCTAGTAAATTCTCTCCAGACCATAACACTCAATTACAGATAAAAGAAGAAAATCCTGTTTTCAAAA CACCTCCACGTTCACAGGAAAGTTTGGAGACAGAGAATCTTTTTGGTGATGTGAAGGGTATTGGGTCTCT TGTGCCAATAAAAGTAAAAGGCAGATCAGCCCATGGAGGATGTGAGCTTGCATCAGTTCTTCAGTTAAAC CCCTGCAGAGTTGCTAAGACAAAGTCTCTGCCCAGCAACCACGATATGTCATTTGAAAATATCCAGTGGA GTGTAGATCCGGGAGCAGACCTTTCTCAATATAAAATGGATGTTACTGTGATAGATACAAAGGACAGCAG TCACTCACGACTGGGAGGAGAGACAGTGGACATGGACTGTACATTGGTCAGTGAAACCATGCTTTTAAAA ATGAAGAAACAAGAACAAAGAGAGAAAAGTCCAAATGGAGACATAAAAATGAATGATAGCTTGGAAGATA TGTTTGATCGGACAACACATGAAGAATATGAATCCTGCTTGGCAGATAACTTCTCCCAGGTACCAGATGA GGAAGAATTGTCTGATACCACAAAGAAACCTAACATACACGGTGATAAACAAGATGGAATCAAGCAGAAA GCATTTGTGGAGCCATATTTTAAAGATAAGGAGAGAGAAACTAGCATACAGAATTTTCCTCATATTGAGG TAGTTCGGAAAAAAGAAGAAAGAAGGAAATTGCTTGGGCACACATGTAAAGAATGTGAAATTTATTACGC AGATTTGCCAGCAGAAGAAAGAGAAAAGAAGTTGGCTTCCTGCTCAAGACACCGATTTCGCTACATTCCA ACCAACACACCAGAGAATTTCTGGGAAGTTGGTTTTCCTTCTACTCAGACGTGTCTGGAAAGGGGTTATA TCAAGGAAGATCTCGATCCTTGTCCTCGTCCCAAAAGACGGCAGCCTTACAACGCAGTCTTTTCTCCAAA GGGCAAAGAACAGAGGACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001134417 |
| Insert Size | 2682 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001134417.1, NP_001127889.1 |
| RefSeq Size | 3135 bp |
| RefSeq ORF | 2682 bp |
| Locus ID | 291787 |
| UniProt ID | B1WC58 |
| Gene Summary | Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (By similarity). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207175 | Rbbp8 (Myc-DDK-tagged ORF) - Rat retinoblastoma binding protein 8 (Rbbp8), (10 ug) |
CNY 11,590.00 |
|
| RR207175L3 | Lenti ORF clone of Rbbp8 (Myc-DDK-tagged ORF) - Rat retinoblastoma binding protein 8 (Rbbp8), (10 ug) |
CNY 13,210.00 |
|
| RR207175L4 | Lenti ORF clone of Rbbp8 (mGFP-tagged ORF) - Rat retinoblastoma binding protein 8 (Rbbp8), (10 ug) |
CNY 14,540.00 |
