Cspg4 (NM_031022) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ng2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207093 representing NM_031022 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCTCAGCCCGGGACACCCGCTGTCAGCTCCAGCCCTGGCCTTGATTCTTACCTTGGCCTTGTTGG TCAGATCTACAGCTCCTGCCTCCTTCTTCGGGGAGAACCACCTGGAGGTGCCTGTGCCCTCAGCCCTGAC CAGAGTAGACTTACTGCTCCAGTTCTCCACATCGCAGCCCGAAGCCCTGCTCCTCTTGGCAGCAGGCCAA ACAGATCATCTCCTGCTGCAGCTCCAGTCTGGACACCTACAGGTCAGACTTGCCCTGGGACAAAATGAGC TGAGTCTGCAGACACCAGCAGACACGGTGCTGAGTGACTCCACAACCCACACCGTAGTGCTCACCGTCTC CAACAGCTGGGCTGTGCTGTCTGTTGATGGAGTGTTGAACACCTCTGCCCCCATCCCAAAAGCATCCCAC CTCAAAGTCCCCTATGGGCTCTTTGTGGGCTCCTCTGGAAGCCTTGACCTGCCTTACCTGAAGGGAATCA GTCGACCCCTGAGGGGCTGCCTCCACTCAGCCATTCTCAATGGCCGCAACCTTCTCCGCCCACTAACCCC CGATGTTCATGAGGGTTGTGCTGAAGAATTCTCTGCTGGTGATGAAGTTGGCCTGGGCTTCTCTGGACCC CACTCACTGGCTGCCTTCCCTGCCTGGAGCACGCGGGAGGAAGGCACCCTGGAGTTTACCCTCACCACTC GGAGTCAGCAAGCACCCCTGGCCTTCCAGGCCGGGGACAAGCGTGGCAACTTTATCTACGTGGACATATT TGAGGGCCACTTGCGGGCTGTGGTTGAAAAGGGTCAGGGTACCATGCTGCTTCGTAACAGCGTGCCTGTG GCTGACGGGCAGCCCCATGAGGTCAGCGTACACATAGATGTTCACCGGCTGGAAATCTCTGTAGATCAAT ACCCTACACGTACTTTCAACCGTGGGGTCCTCAGCTACCTGGAGCCTCGTGGCAGTCTCCTCCTTGGGGG GCTCGATACAGAGGCCTCTCGCCACCTCCAAGAACACCGTCTGGGCCTGACACCGGGGGCTGCCAACATC TCCCTGGTAGGCTGCATAGAAGATTTCAGTGTTAATGGCAGGAGGCTGGGCCTCCGGGACGCCTGGCTGA CCCGTGACATGGCAGCAGGCTGCAGGCCTGAGGAGGATGAGTATGAGGAAGAGGTCTATGGCCCGTTTGA AGCTTTCTCCACCCTGGCGCCCGAAGCCTGGCCAGTCATGGATCTGCCAGAGCCGTGTGTTCCTGAGCCG GGACTGCCTGCCGTCTTCGCGAACTTCACGCAGCTGCTCACCATTAGTCCCCTGGTCGTGGCCGAGGGTG GCACAGCCTGGCTTGAGTGGCGGCACGTGCAGCCCACGCTGGACCTGACAGAGGCGGAGCTGCGCAAGTC CCAGGTACTGTTCAGCGTGAGCCAGGGTGCACGCCACGGCGAGCTGGAGCTAGACATCCCGGGAGCCCAA ACCCGAAAAATGTTTACACTGTTGGACGTGGTGAACCGAAAGGCTCGCTTTGTTCACGATGGCTCTGAAG ACACCTCTGACCAGCTGATGCTGGAGGTATCAGTGACTTCTCGGGCACCTGTGCCCTCTTGCCTGCGGAG GGGCCAAATTTACATTCTCCCCATCCAGGTAAACCCCGTCAACGACCCACCTCGCATCGTCTTCCCGCAC GGCAGCCTCATGGTGATCCTGGAGCACACACAGAAGCCTCTGGGACCCGAGATTTTCCAGGCCTATGACC CAGACTCTGCTTGTGAGGGTCTCACCATCCAGCTTCTTGGTGTCTCCGCTAGTGTCCCTGTGGAACACCG AGACCAGCCCGGAGAGCCAGTGACTGAGTTTTCCTGTCGAGACCTAGAGGCAGGCAACATAGTCTATGTC CACCGTGGCGGGCCTGCACAGGACCTGACATTCCGGGTCAGTGATGGAATGCAGGCCAGTGGGCCAGCTA CACTGAAGGTCGTGGCCGTCCGGCCAGCCATACAGATCCTCCACAACACAGGGCTGCGCCTGGCCCAGGG CTCTGCTGCAGCCATCTTGCCTGCCAACCTGTCGGTAGAAACGAATGCAGTAGGACAGGATGTGAGCGTG CTGTTCCGAGTCACTGGCACCTTGCAGTTTGGGGAGCTGCAGAAGCAGGGGGCCGGAGGGGTAGAGGGCA CCGAGTGGTGGGATACACTGGCCTTCCACCAGCGCGATGTGGAGCAAGGCCGAGTGAGGTACCTGAGTAC TGACCCACAACACCACACCCAAGACACAGTGGAGGACCTGACCTTGGAGGTGCAGGTGGGCCAGGAGACA CTGAGCAATCTGTCTTTCCCAGTGACCATCCAGAGAGCCACAGTATGGATGCTGCAGCTTGAGCCTCTGC ATACACAGAACCCTCATCAGGAAACCCTCACCTCAGCCCACCTAGAGGCTTCCCTGGAGGAGGAGGGAGA AGGAGGACCATACCCTCATATCTTCCACTATGAGTTGGTTCAGGCCCCCAGGAGAGGCAACCTCCTGCTC CAGGGTACAAGGCTGTCAGATGGCCAGAGCTTCAGCCAGAGTGACCTGCAGGCCGGTCGTGTGACCTACA GGGCCACAACGCGGACCTCTGAGGCAGCTGAGGACTCCTTCCGTTTCCGGGTCACATCTCCACCACATTT CTCCCCGCTCTACACCTTCCCTATCCACATTGGCGGTGACCCAAACGCTCCTGTCCTCACTAACGTCCTG CTCATGGTACCCGAGGGAGGGGAGGGAGTGCTGTCTGCTGACCACCTCTTTGTCAAGAGTCTCAACAGTG CCAGCTATCTCTATGAGGTTATGGAGCAGCCCCACCATGGCAGCTTGGCTTGGAGGGACCCAAAAGGTAG AGCCACCCCAGTAACATCCTTTACTAATGAGGACCTGCTACACGGCCGACTGGTCTACCAGCACGATGAC TCTGAGACCATAGAGGATGATATCCCATTTGTGGCCACACGCCAGGGCGAGGGCAGCGGTGACATGGCCT GGGAGGAGGTGCGTGGTGTCTTCCGAGTGGCCATCCAGCCTGTGAACGATCACGCCCCTGTGCAGACCAT CAGCCGTGTCTTCCACGTGGCCCGGGGCGGACAGCGGCTGTTGACTACAGATGATGTGGCCTTCAGTGAT GCTGATTCGGGCTTCAGTGACGCGCAACTGGTGCTGACCCGCAAGGACCTCCTCTTTGGCAGCATCGTGG CTATGGAGGAGCCCACGAGGCCCATCTACCGTTTCACCCAAGAGGATCTCAGGAAGAAGCAAGTCCTGTT TGTGCACTCAGGGGCCGACCATGGCTGGCTCCAGCTACAGGTGTCTGATGGGCAACACCAGGCTACTGCC ATGCTGGAGGTGCAGGCCTCAGAGCCCTATCTCCACGTAGCCAATAGTTCCAGTCTTGTGGTTCCTCAAG GAGGCCAGGGCACCATCGACACAGCCGTGCTCCACCTGGACACCAACCTAGATATACGAAGTGGGAATGA GGTCCACTACCATGTCACAGCTGGCCCTCACTGGGGACAGCTGCTCCGGGATGGCCAGTCAGTCACCTCC TTCTCGCAACGGGACTTGCTGGATGGGGCCATTCTCTACAGCCACAATGGCAGCCTCAGCCCGCAAGACA CCCTGGCCCTTTCTGTGGCAGCAGGGCCAGTACACACTAGCACCGTCCTACAAGTGACCATTGCCCTAGA GGGCCCCCTGGCTCCACTACAACTGGTGCAGCACAAAAGGATCTATGTCTTCCAAGGGGAGGCAGCTGAG ATCAGAAGGGACCAGCTAGAGGTAGTCCAGGAGGCAGTGCTGCCTGCCGACATCATGTTCTCGTTGAGAA GCCCCCCGAACGCTGGCTACTTGGTGATGGTGTCCCACGGTGCTTCAGCAGATGGGCCACCCAGCCTGGA CCCTGTGCAGCGCTTCTCCCAAGAGGCAATAAATTCAGGCCGGGTTCTCTACTTGCACTCTCGCCCTGGA GCCTGGAGTGATTCCTTCTCCCTGGATGTGGCCTCGGGTCTGGGCGATCCTCTCGAAGGCATCTCTGTGG AGCTGGAAGTGTTGCCCACCGTCATCCCCCTGGATGTTCAAAACTTCAGCGTTCCTGAGGGTGGCACTCG TACGCTGGCCCCTCCGCTGATCCAAATCACTGGGCCCTACTTGGGGACACTGCCAGGCCTTGTCCTACAG GTGCTAGAGCCACCGCAGCACGGGGCCCTGCAGAAGGAGGACCGTCCTCAAGATGGGACCCTCAGCACTT TCTCCTGGAGAGAGGTGGAAGAGCAGCTGATCCGATACGTGCATGATGGGAGTGAGACGCAGACGGACGG CTTCATCCTGCTAGCTAATGCCTCAGAGATGGATCGCCAGAGCCAGCCCATGGCCTTCACTATCACCATC CTCCCTGTTAATGACCAACCCCCTGTCATCACCACAAACACAGGCTTGCAGATCTGGGAGGGGGCTATTG TGCCCATCCCTCCTGAGGCCCTCAGGGGAATAGACAGTGATTCAGGGCCGGAAGACTTGGTCTACACCAT CGAGCAGCCCAGCAATGGACGGATAGCCTTGAGGGTAGCACCAGACGCCGAGGCCCACCGCTTCACACAG GCCCAGCTGGACAGCGGGCTTGTGCTGTTCTCACACAGAGGAGCCCTGGAAGGAGGCTTCCACTTCGACC TCTCTGATGGCGTACACACTTCTCCTGGACATTTCTTCCGAGTGGTAGCCCAGAAGCAGGTACTCCTCTC CTTGGAGGGCAGCCGGAAGCTGACTGTCTGTCCGGAGTCTGTGCAGCCACTCAGCAGCCAGAGCTTGAGT GCCAGCTCCAGTACAGGCTCCGACCCTCGTCACCTGCTCTACCAGGTGGTAAGAGGCCCCCAGCTCGGCC GACTCCTCCATGCCCAGCAAGGAAGTGCAGAGGAGGCTTTGGTGAACTTCACCCAGGCTGAGGTGAATGC TGGGAATATTCTGTATGAGCATGAGATATCCTCTGAGCCCTTCTGGGAAGCCCACGATACCATTGGTCTC CTGCTGTCTTCATCACCTGCGAGGGATCTGGCTGCCACCCTGGCTGTGACTGTGTCTTTCGACGCTGCCT GTCCCCAGCGGCCTAGCCGTCTCTGGAGGAACAAAGGTCTTTGGGTCCCTGAGGGCCAGCGGGCCAAGAT CACTGTGGCTGCCCTTGATGCCGCCAACCTCCTAGCCAGTGTGCCAGCATCTCAGCGCGGTCGGCATGAT GTACTCTTCCAGATCACACAGTTCCCCACCCGGGGTCAGCTCCTGGTGTCCGAGGAGCCACTCCATGCCA GGAGACCCCACTTCCTGCAATCTGAGCTGACTGCAGGACAGCTGGTGTATGCCCACGGTGGTGGGGGCAC CCAGCAGGATGGCTTCCGCTTCCGTGCCCACCTCCAGGGACCAACGGGAGCTTCCGTGGCAGGACCCCAA ACCTCTGAGGCTTTTGTCATCACTGTGAGGGACGTGAATGAGCGACCCCCTCAGCCACAGGCCTCCATTC CTCTCCGCATCACCAGGGGCTCACGAGCCCCTGTCTCTCGAGCCCAGCTGAGTGTCGTAGACCCAGACTC AGCTCCAGGGGAAATTGAGTACGAAGTACAGAGGGCACCCCACAATGGCTTCCTGAGCCTGGCTGGGGAC AACACTGGGCCTGTGACTCACTTTACACAGGCTGATGTGGATGCAGGGCGACTGGCCTTTGTGGCAAATG GGAGCAGTGTGGCTGGCGTCTTCCAGTTGAGCATGTCTGATGGAGCCAGCCCCCCAATACCCATGTCGCT GGCTGTGGATGTCTTGCCATCCACCATTGAGGTGCAGCTGCGAGCGCCCCTGGAGGTGCCTCAAGCCCTA GGACGTTCCTCACTGAGCAGGCAGCAGCTCCAGGTGATTTCAGATCGTGAGGAGCCAGATGTAGCTTACC GCCTCACTCAGGGGCCCCTGTATGGGCAGGTACTAGTGGGGGGGCAGCCCGCTTCAGCCTTCAGCCAGCT GCAGGTAGACCAGGGGGACGTGGTCTTTGCCTTCACCAACTTTTCTTCCTCTCAGGATCATTTCAAAGTC CTGGCTCTTGCTAGGGGTGTGAATGCATCAGCTACTGTAAATGTCACAGTGCAGGCCCTGTTGCATGTGT GGGCCGGTGGGCCATGGCCTCAGGGTACCACCTTGCGCCTTGACCCCACTGTGCTCGATGCCAGTGAACT GGCCAACCGCACAGGCAGTATGCCCCGCTTCCGGCTCCTGGAAGGACCCCGGTACGGCCGTGTGGTTCGT GTGTCCCAAGGCCGAGCAGAATCTAGGACCAACCAACTTGTGGAAGATTTCACACAGCAAGACCTGGAAG AGGGAAGGCTGGGGCTAGAGGTGGGCAGGCCAGAGGGCAGGTCCACTGGCCCAACAGGTGATAGACTTAC CCTGGAGCTGCAGGCAACGGGTGTCCCACCTGCTGTGGCCTTGCTGGACTTTGCCACTGAGCCTTACCAT GCAGCCAAGTTCTATAAGGTGACCCTACTCAGTGTCCCTGAGGCTGCTCGTACAGAAACAGAGAAAACAG GAAAAAGCACCCCCACTGGCCAGCCAGGCCAAGCAGCATCTAGCCCCATGCCCACTGTAGCCAAAAGTGG TTTCTTGGGCTTCTTAGAGGCCAACATGTTCAGCGTCATCATCCCCGTGTGCCTGGTCCTCCTGCTCCTG GCTCTCATCTTGCCTCTGCTTTTCTACCTCCGCAAACGCAACAAGACAGGTAAGCATGATGTCCAGGTGT TGACCGCCAAACCCCGCAATGGCCTAGCTGGTGACACAGAGACCTTTCGAAAGGTGGAGCCAGGCCAAGC CATTCCACTCACAACTGTACCTGGCCAGGGGCCCCCACCCGGAGGCCAGCCTGACCCAGAACTATTACAG TTTTGCCGGACACCCAATCCTGCCCTCAGGAATGGCCAGTACTGGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031022
Insert Size	6981 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031022.1, NP_112284.1
RefSeq Size	8074 bp
RefSeq ORF	6981 bp
Locus ID	81651
UniProt ID	Q00657
Gene Summary	extracellular matrix protein secreted by many cell types; member of the large chondroitin sulfate proteoglycan (CSPG) family; may be involved with angiogenesis -dependent processes such as wound healing or tumor growth [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.