Shroom2 (NM_001047893) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ab2-404; Apxl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207087 representing NM_001047893 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCGCCGAGCCCCGTGCACGGCCCGAGCGCTTGGCTGAAGCTGAGGCGCGGGCGACCGACGGCG TCCGTCTGGTGGAGGTGCAGCTGAGCGGCGGTGCCCCTTGGGGCTTCACCTTGAAGGGCGGCCGCGAGCA TGGTGAGCCTCTGGTCATCACCAAGATCGAAGAAGGAAGCAAAGCTGCATCAGTGGACAAGCTTCTTGCT GGAGATGAGATAGTGGCCATCAATGATGTCACTCTCTCAGGGTTCAGACAAGAAGCAATTTGCCTGGTGA AAGGCTCCCACAAGACCCTCAAGCTTGTGGTCAAAAGGAAAAGTGATCCAAGTTGGAGGCCTCACTCCTG GCATGCTACCAAGTACTTCGATGTCCACCCTGAGCCACCAGTCTCACTGTTTCTGAACTCCAGCAGTTCC CCTTCCTGGAAGAGCCAGCACCAGGCTAGTTCTTCCTCCCATGATTTATCTGGCTCATGGGAGCACACCA GCCTACAGCGCACTTCTGACCACTTCAGCTCCATGGGCAGCATAGACAGCCTGGACCACAGCTCCCAGCT CTACCCATCTGGTCACCTCTCATCTGCCAAGTCCAACAGCAGCATTGACCACCTGGGTGGCCATAGCAAG CGAGACTCGGCTTATGGCTCCTTTTCCACCTGTTCCAGCACCCCTGACCACACCTTGCCCAAGGCTGACG CCTCATCCACTGAGAACATTCTCTACAAAGTTGGCCTTTGGGAGGCTTCCAGGCCAGGCAGCAGCCGACA AAGCCAGTCTACAGGTGATCCTCAGGGACTACAGGACAGGCCATCGTCTTTCTTACCTAGGGTTCCTGGT AACAGTAGTAAAAGCCCAAGGCCGGAGGATAATATTGAGCCCAAAATAGCAACTTCTGGGAGATCCAATT TTGGGCCTGTCTGGTATGTTCCTGATAAGAAAAAAGCCCCTTCTCCCCCACCTCTTGGACTTCCTTTGCG CAGTGATAGCTTTGCTGTGGCAGCCAGGGGCCATGAAAAGGCTCGGGGCCCTCCATTCTCAGACTTGGCC AGCATGCAGCACTTTACCACCCTGCCTCATGTACAACCCCGGGGAGACCGCAGAATGGAAACTACTGATC GACAGTGGAAGCTTGCACACCCAAGCAGTGGCAAAGAAATAGGTAATGTGGGTTACCAGCCAGAAGGCCA CCTGGATTGCCGTTGGCTGTGCTCTGATGATAGGGCAGGTAGGCCCTCAGGAGCTCCAGGAAGACACCAG TTCTCCCTGTCCAGCACAGATGTGCACTTTCTGAAGTCTTACCATGGGAGCCAACACCCACAGCCGTGCA GTGATGAGAGCCCAAGATTCCCCTCATCACCAAGGGAATTGCTTCGTATAACTCCTAGTGGATGTCTGCA GGAGCCTCCTGAACTGTCTCAGGATGACAACCCTGCTCAGGTGAGGTGGCCCGGTTCTGTTAACCAGAAG CTAGATGACAGAGGGCGGAGCCACTATTTTTCTGTGCCCCACAGGCAGCCAGTGCACGGGAGTGCCCACG TTTTGATACCCCGAAGTGACTATTGGCATTCAGACACAACTCCTGTGGACCTTGAGTGTCCTCTCTTACG CCCAGACCAGAGGGGCTACCCACAGCAGTATGAGGAGACCCCAGCCTCTCATGAGAGAGGCGGGTATCAA CAGCTAAATGCAGGGATTGAAGGCTGCTGCTCAGGAATCCATGAGCCTCCTAGAGCCAGCCACACTGTGA GAGCTGGTCTGCAGTGTCCTGGCAATGACTTCAAGTTAGTGGATGCAGAGAGTGGAAGAATTTCTCGTCA GAGAACACCCATGCTGCACTCTCTGACCCAAGATGGTGCATGGAGGCCTGGGAACAGCAAGGATTGTGGA AATGAGAAGCCACCACTGCTTGATGCCCAGGTGGGTAAACCCACACGGAGGAGTGACCGTTTTGCCACAA CACTAAGGAATGAGATTCAGATGCGCAGAGCAAAGCTACAGAAGAGCAAAAGCACAGTGACATTAGCTGG AGATAGTGAAGCTGAAGACTGTGCTGGAGACTGGAGAGTTGATGTGGGGGCTGTTCCAGAAGGTTCCTTC CCCAGCACCTATAAAGAGCACCTGAAGGAGGCCCAGACACGTGTTCTAAAGGCCACCTCTTTCCAACGCC GAGATTTAGATCCCACCCCAGCAGATCAGTATCCAGGACCACCAGAACATAGGACTTGTGACCACAGTGC CTCATCTTCTTTATCTTCTTTCCCTGGGGAGCCAGACTCTGCCCCTCGCCTCTGTGAAGCAGGTCTGGCC AAGCCACCCTCTTCTGCAGGTGGTGTTCCCCACATTCTTCGAATTGGAGGCCGGAAACGGTTCACAGCAG AGCAGAAACTAAAGTCCTATTCTGAGCCAGAAAAAATTAATGAAGTGGGGCTTTCAGGGGACCACAGTCC TCATCCTACTATTAGGACATCTGAGGATTCTGTGGGTACATTTGCTGATAGGTGGAAGTTTTTTGAGGAA ACAAGCAAATCTCTTCTCCAGAAGCCAGGCCATAGGCAAGCTTTCTGTGGGATCCCAAGAGAAAAGGCTG AGAGGCCTCAAACACAGGGCCATGAGTGTGAGAGTACAGAGCCCTGGTTTCAGAAGAGGTCACGGGCCAC CTCTTGTGGAGAGATCCTCAGTGAAGACAGAAAAGTAGAAAAGGCCTCAGAGAAATTGAACCCACCCAGA AGGCTTGGAACCTTTGCAGAATATCAAGCATCTTGGAAGGAGCAGAAGAAATCTCTAGAAGCCAGGAGTT CTGGACGATACCATTCAGCAGATGATATTCTGGATGCTGGCCTAGATCAGCAGCAGAGGCCACAGTACAT TCATGAACGGTCTCGTTCATCACCGTCCACAGATCACTATTCACAGGAAGTGCCTGTTGAACCAAACAGG CAGGCAGAGGACTCTGGTGGCCAGAAAGAAGCACTTCTTTGTACACTGCAAGCTGAAGAGGGATGCTCTG CTCCAAGCTCCTCTGTGCTCAGCAGTGCCCAGCCGCAAGACAGCCAGCATGTGAATGAGGACCCAACTTC CCCTCAACCAGAAGCCCAGCTCTCCTCCAAGTGTCAACACTTACAGACATCAACCATGGAAACTTCTCGC TCCCCTTCACCTCAGTTTGCCCCACAGAAGCTGACAGACAAGCCTCCCCTGCTTATCCATGAAGACAATT CAGCAAGAATTGAGCGGGTGATGGACAACAACACCACTGTGAAGATGGTGCCCATAAAAATTGTGCACTC AGAAAGCCAGCCTGAGAAGGAAAGCCGCCAGAGTCTCGCTTGCCCAGCCGAGCTGCCCGCACTGCCCAGT GGGCTGGAGAAGGACCAGATCAAGACACTGAGTACATCAGAGCAGTGCTACTCGCGCTTTTGTGTGTACA CACGACAGGAGGTGGAAACCCCTCATAGAGCCCGCCCTCCAGAGCCCCAGCCACCTAGCACCCCTGCACC TCCTGTCAGAGATAGCTGTTCCTCTCCTCCCTCACTCAACTATGGGAAGGCCAAGGAGAAGACCGTGGAC GACTTGAAGTCTGAAGAATTAGCCAGAGAGATTGTGGGAAAGGACAAGTCTTTGGCTGACATCCTGGACC CCAGCGTGAAAATCAAAACTACCATGGATCTGATGGAGGGAATTTTCCCCAAAGATGAGTACCTCCTAGA GGAAGCTCAGCAACGGAGGAAGCTGCTCCCCAAAGTCCCCTCACCCAGAGTCACAGAGGACAAGAAACAG GACCCAGGTATGCCAGGGGTTGTGTCCTTGGCCACAAATTCTACCTATTATAGCACATCAGCCCCCAAGG CAGAACTGCTTATCAAGATGAAGGACCTACAGGAGCCTGAGGAGTATTCAGCAGGTGACTTGGATCATGA CCTTTCCATTAAGAAGCAAGAGCTCATCGACAGTATCAGCCGCAAGCTGCAGGTGCTCCGAGAAGCACGT GAGAGTCTGCTGGAGGACATCCAAGCCAACAATGCTCTCGGGGATGAAGTGGAAGCCATTGTGAAAGATG TCTGCAAGCCCAATGAGTTTGACAAGTTCCGGATGTTCATTGGAGACCTGGACAAAGTGGTGAACCTCCT GCTGTCACTGTCAGGACGCCTGGCCCGTGTGGAAAATGCCCTTAATAATTTAGATGACAGTCCTTCTCCT GGAGATCGGCAGTCACTGTTGGAGAAACAGAGAGTCCTAACTCAGCAGCATGAGGATGCGAAGGAGCTTA AAGAGAACCTGGACCGTCGTGAGCGCATTGTGTTCGACATCCTGGCCACCTACCTCAGCGAGGAGAACCT GGCTGACTATGAGCACTTCGTGAAGATGAAGTCAGCCCTCATCATTGAGCAGCGAGAGCTGGAAGATAAA ATCCACCTGGGTGAAGAACAGCTCAAGTGTTTGTTTGACAGCCTGCAGCCTGAGAGAAGCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001047893
Insert Size	4476 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001047893.3, NP_001041358.2
RefSeq Size	6987 bp
RefSeq ORF	4476 bp
Locus ID	317435

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