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Adhfe1 (NM_001025423) Rat Untagged Clone
CAT＃: RN206998
Adhfe1 (untagged ORF) - Rat alcohol dehydrogenase, iron containing, 1 (Adhfe1), (10 ug)
CNY 6,080.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN206998 representing NM_001025423
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCAGCTGCACGCGCTCGAGTGACACATTTGCTGAGGCACCTGCAAAGCACAGCATGCCAGTGTC CAACTCATTCTCATACCTACTCCCAAGTCCCTGGACTCTCACCTTCAGGAAAAACAACGGATTATGCATT TGAGATGGCGGTCTCGAACATTAGATATGGAGCAGGGGTTACAAAGGAAGTGGGCATGGATCTACAGAAT ATGGGAGCTAAGAATGTCTGCCTGATGACAGACAAGAACCTCTCCCAGCTCCCTCCTGTCCAAATAGTTA TGGATTCCCTATCGAAGAATGGCATTAGTTTTCAGGTTTATGACAACGTGAGGGTGGAGCCTACAGACGG AAGCTTCATGGATGCGATTGAATTTGCTAAAAAGGGAGCCTTTGATGCCTATGTTGCTGTGGGTGGGGGC TCCACCATGGACACCTGTAAAGCCGCTAATCTGTACGCATGCAGCCCTCACTCTGAGTTCCTTGATTATG TCAATGCCCCCATTGGGAAGGGGAAGCCAGTAACTGTGCCTCTTAAACCTCTGATCGCAGTTCCAACTAC CTCGGGGACTGGCAGTGAGACTACCGGGGTCGCCATTTTTGACTATGAACATTTGAAAGTAAAAACTGGA ATTGCTTCACGGGCCATCAAACCCACCCTGGGGCTGGTTGATCCCCTGCATACTCTCCACATGCCTTGCC AGGTGGTTGCCAACAGTGGCTTCGATGTCCTTTGCCATGCTCTGGAGTCATACACCGCCATTCCCTACAG CATGAGGAGCCCCTGCCCTTCCAATCCCATCCAACGACCAGCATACCAGGGTAGCAACCCAATCAGTGAC ATCTGGGCAGTCCATGCACTGCGCATCGTTGCCAAATATCTGAAGAGGGCTGTCAGAAACCCTGACGATC TGGAAGCAAGGTCTAGCATGCACTTGGCAAGCGCCTTCGCTGGCATTGGCTTCGGAAACGCCGGTGTTCA TCTGTGCCATGGCATGTCTTACCCAATTTCAGGTTTAGTGAAGACATACAAAGCCAAGGAATACAATGTG GATCACCCTCTGGTGCCCCATGGCCTCTCTGTGGTGCTCACCTCTCCCGCAGTGTTCACCTTCACAGCCC AGATGTTTCCAGAGCGGCACCTGGAGACGGCAGAAATATTAGGAGCCAACATTCGCACCGCCAAGATCCA AGATGCCGGGCCTGTGTTGGCAGATGCTCTCCGAAAATTCCTATTTGACCTAAATGTTGATGACGGTCTC GCTGCCCTTGGTTATTCTAAAGATGACATTCCTTCACTGGTGAAAGGAACACTGCCCCAGGAAAGGGTCA CGAAGCTTGCGCCGCGTGCCCAGTCAGAGGAAGATTTGTCTGCTTTGTTCGAAGCATCAATGAAACTGTA CTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001025423 |
| Insert Size | 1404 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025423.1, NP_001020594.1 |
| RefSeq Size | 1884 bp |
| RefSeq ORF | 1404 bp |
| Locus ID | 362474 |
| UniProt ID | Q4QQW3 |
| Gene Summary | Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR206998 | Adhfe1 (Myc-DDK-tagged ORF) - Rat alcohol dehydrogenase, iron containing, 1 (Adhfe1), (10 ug) |
CNY 6,080.00 |
|
| RR206998L3 | Lenti ORF clone of Adhfe1 (Myc-DDK-tagged ORF) - Rat alcohol dehydrogenase, iron containing, 1 (Adhfe1), (10 ug) |
CNY 7,890.00 |
|
| RR206998L4 | Lenti ORF clone of Adhfe1 (mGFP-tagged ORF) - Rat alcohol dehydrogenase, iron containing, 1 (Adhfe1), (10 ug) |
CNY 8,740.00 |
