Pik3c2g (NM_053923) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206800 representing NM_053923 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATACAATTGGCAAACAGAGCCAAACCGTGCTGAACCACAGGAAGGTGGACATGATCACCAGCAGT GTCACCATGCAGACCAGCACCTTTCTTCCAGGCAAGTCAGGTTGGGTTTTGATCAGCTTGTGGAAGAGCT CAGTAACAAAACTCCACTGCCTGAGGATGAAAAAGAAGGCACGTGTTTTGTACCAGATACACCAAACTTG GATTCAAAATGGCAATCCATATATGGACCCCACCCAAGGCACTTCAATGAATTCACTTCTCAGAGTCCCC ACTTCTCCCAGCTTCCTTTTGGAAAAGCATCAGCCATTGGTTTTAATCCTGCTGTATTACCTGCACATCA GTTCATTCATGAGGGAGCCTCCTGGAGAAATCCCACAAGAAAATATCATGGTGGTGAGGATCCCAGGTTC AGTGCTCTAACTCCGTCATCCACTGGCTTGGATAAATGTCATCAACAAGGACAATCAGGGACCGAACATT GTAACTATTATGTGGAACCTGAAAACAATGTTCCCCATCATTATTCACCCTACTCAATGGACTCCATACC CGATAGTGAGGAAAAAGGAAGTGGAGATGCGGATCTTGTAGAACCTTCTCTGGTGTTCTCTAAAGACTCC TTTCTACCCAGGGCATCGGAGAACATGTCAGTGGAAAGCACAGAGCCCATTGGTTGCCCCCTTGAAATAG TTGAAGCACCCCAAGGGAGTAACAAGAGCCTCGCCTCCTTTTGCAACAATGTAACAAAAATAAGAGGACT ATATCATGCAAGTGACACTAATTCCAATTCCGGAAAGATCTGGGCCATCACCACAGCCTATCCATCTCGG CTCTTCGCTGACACCCAGTTCAGAGTTAAAATTTCCACTGATAACTCGGCACAACTTCTTCTTCTTAAGC CACCCGCTAATTATCTTGTCAAAGACCTAATTGCCGAAATTCTACTTTTATGTGCAAATGAGCAGCTTTC CCCCAAAGAGTATCTTCTAAGTATATGCGGTTCTGAGGAATTTTTACAGACGGATCACTGTCTAGGGAGC CACAAAATATTTCAGAAAAGTAAATCTGTCATTCAACTCCATCTCCAGAGAAGCAGGGACACTCCAGGAA AATTATCCCGGAAGAGGGATGATGACCGCAGTCGGGTCCATCTGAACCAACTTCTAGAATTTACACATAT TTGGAAAATATCCAGACAATGCCTCTCCACAGTAATGAAAAGCTACAACCTCCATGTCGAGCACCTGTTG AAAACCCAGGAAGATGTGGAGGAGAAACCTCTGTCATCCATGTTTTCCTGTGGCCGACACCCTCCTCAGC CACATGGGAATGACATTATTGAAGATGTTAGAAACATATGCAGTGTTCTGGGGTGTATTGAAACCAAACA AGTTTCAGATGCAGTAAAAGAACTAACTCTAATTCTGCAGAGACCATCACAGAATTTTCATCAGAATTCA GAGACTTCAAAAAAAGGCTTCATAGAGAACGTGACATCGGAACTGTCGAGGTCCCTCCATCAGCTGGTTG ACGTGTACTGCAGTAGCTTTTGTACAGATTTCCGGCCTGCGCGCGCACCTGGAGGCGTCTCCCGCGACCA CGCTGGGCTCCACTCCCACCTGAGCTTCACGGTGTGTTCCCTGCACAATGTTCCAGAAACTTGGGCACAC AGCTACAAAGCATTTTCATTTTCCTGCTGGCTCACATATGCTGGGAAGAAGCTGTGCCAAGTGAAAAGCT GCAGATCCCTGCCAGTCACAAAGTCATTCTCTTTTTCGGTGAACTGGAATGAAATAATCAATTTTCCTCT TGAGATAAAGTCACTTCCAAGAGAATCCATGCTCGTTATAAAGCTGTTTGGGATTGACAGTGCCACCCAC AGCGCAAATCTGCTGGCCTGGACCTGCCTTCCACTATTTCCAAAAGAAAAGTCTCCGCTGGGGTCTAGGC TTCTCAGCATGACACTACAGAGTGAGCCTCCTATAGAAATGATGGCTCCAGGAGTATGGGATGGGAGCCA GCCTACCCCACTGACCCTGCAGATAGATTTTCCAGCTGCCACGTGGGAGTACGTGAAACCTGAGACTGAA GAGAACAGAACTGACCACCAAGAGCCTCCAAGAGAGTGTTTAAAACACATCGCCAGACTCTCCCAAAAGC AGCCTCCCTTGCTACTTTCTGTGGAAAAGAGGAGATATTTGTGGTTTTATCGTTTCTACTGCAACAATGA GAACTCCTCTCTCCCTCTGGTCTTGGGCAGCGCCCCTGGTTGGGATGAAGGGACAGTTTCGGAAATGCAT GCCGTCTTGAGAAGGTGGACATTTTCCCATCCGTTGGAAGCTCTTGGCCTTTTGACTTCCAGGTTTCCAG ACCAAGACATTCGTGAAGTTGCCGTTCAACAGTTAGACAACTTCTTGACCGATGAGCTGCTGGACTGCCT CCCACAGCTAGTTCAGGCTGTCAAGTTTGAGTGGAGTCTCGAAAGTCCCTTGGTGGAACTCCTGCTTCAT CGATCCTTGCAAAGCATCCGAGTGGCTCACCGCCTGTTCTGGCTGCTGCGGGATGCACAAGGTGAAGACT ACTTTAAAAGCTGGTACCAGGAGCTTTTGGCCGCTCTCCAGTTCTGTGCAGGAGAAGCCCTGATCGAAGA GCTTTCCAAAGAGCAGAAACTTGTCAAACTCCTGGGTGATATTGGAGAAAAAGTGAAGTCGGCTGGCGAT GCTCAGAGAAAGGATGTGCTAAAGAAGGAGATTGGCAGTCTAGAAGAATTCTTTAAAGATATAAAGACTT GCCATCTTCCTCTGAACCCGGCCCTGTGCGTAAAAGGAATTGATCGGGATGCATGTTCATATTTCACATC TAATGCCTTGCCATTGAAGATCACTTTCATCAATGCTAATCCAATGGGCAAAAATATCAGTGTTATTTTT AAGGCCGGCGACGATCTTCGGCAGGATATGCTTGTTCTGCAGATTATTCAAGTGATGGACAACGTTTGGC TTCAGGAGGGCCTCGATATGCAAATGATCATTTATGGATGTCTAGCCACAGGAAAGGCTCAAGGATTCAT AGAGATGGTGCCTGATGCTGTAACGCTTGCCAAGATCCATCTGCACTCTGGGCTGATAGGACCCCTGAAA GAAAACACCATCAAGAAGTGGTTCAGTCAGCACAACCACTTAAAGGAAGATTATGAAAAGGCCTTGAGGA ACTTTTTTTACTCTTGTGCTGGCTGGTGTGTGGTGACATTCATCTTGGGAGTCTGTGACCGACATAATGA CAATATCATGCTGACAAAGTCAGGCCACATGTTTCATATTGACTTTGGAAAATTCTTGGGTCACGCACAA ACATTTGGCGGTATAAAAAGGGACCGAGCGCCTTTCATTTTTACTTCAGAGATGGAGTACTTTATTACGG AGGGTGGGAAAAACACACAGCATTTTCAAGACTTCGTGGAACTCTGCTGCAGAGCCTACAACATTGTGAG GAAGCACAGCCAACTGCTCCTGAGCCTTCTAGAAATGATGCTGCATGCCGGGCTTCCTGAGCTGAGGGGG ATTGAAGACCTGAAATACGTACACGACAATCTCCGGCCACAAGACACAGACCTGGAAGCCACAAGTCATT TTACCACGAAGATAAAGCAGAGTCTGGAGTGCTTCCCAGTTAAACTGAATAACCTGATCCACACGCTTGC ACAGATGCCAGCCTTCAGCCTTGCCAGACCTGCCCCTCAGACTCCTCCCCAGGAGTGCTGCGTCCTGAAT AAAACCAGGACAATTCAGAGAGTCACAATTTTAGGGTTCAGCAAGACACACAGCAACCTGTACCTGATCG AGGTGACACGCAGCGACAACAGGAAAAACCTGGCCAAAAAGTCCTTCGAGCAGTTTTACAGACTTCACAG CCAGATTCAGAAGCAGTTCCCCTTGTTGACTCTCCCAGAGTTTCCTCACTGGTGGCATCTACCTTTCACA GACTCGCACCATGAGAGAATCCGAGATCTGAGTCACTACGTGGAACAGGTGCTGCACGGATCTTACGAAG TCGCAAACAGTGATTGTGTACTCAGTTTTTTTCTCTCTGAACATATACAACAGACCCTTGAAGACTCTCC ATTTGTGGACCCAGGTGACCATTCTCCAGACAAGAGCCCCCAGGTGCAGTTGCTGATGACCTATGAGGAC ACAAAGCTCACCATCCTAGTGAAACACTTGAAAAACATCCATCTCCCAGATGGCTCAGCGCCCAGCGCAC ATGTTGAAATTTATCTTCTGCCACATCCCAGTGAAGTTCGCAGGAAGAAAACAAAGTGCGTTCCAAAATG CACTGACCCAACTTACAATGAAATTGTGGTATATGATGACGTCTCAGGACTTCAGGGACATGTTTTAATG CTCATTGTGAAGAGCAAAACTGTATTTGTGGGAGCGGTTAACATTCAGCTCTGCAGTGTTCCCCTCAATG AAGAAAAGTGGTACCCATTAGGGAACAGTATCATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053923
Insert Size	4518 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053923.1, NP_446375.1
RefSeq Size	5990 bp
RefSeq ORF	4518 bp
Locus ID	116720
UniProt ID	O70173
Gene Summary	catalyzes the addition of a phosphate group to the 3' position for phosphotidylinositol and PtdIns 4-P substrates, but not for PtdIns 4,5-P2 [RGD, Feb 2006]

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