Myh4 (NM_019325) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206669 representing NM_019325 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCGACGCCGAGATGGCCGTTTTTGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGC GAATCGAGGCTCAGAACAAGCCTTTTGATGCCAAGTCATCAGTGTTTGTGGTGGATGCTAAGGAGTCCTA TGTGAAAGCAACCGTGCAGAGCAGGGAAGGGGGGAAGGTGACGGCCAAGACCGAAGGTGGAGCTACTGTC ACAGTTAAAGAAGATCAAGTCTTCTCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA TGACCCACCTCCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTACAC TTATTCGGGGCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGTTGCCGGTGTACAACCCCGAGGTGGTG GCAGCTTACCGAGGCAAAAAGCGACAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGATAATGCCTACC AGTTCATGCTGACGGATCGTGAGAACCAGTCCATCCTGATTACTGGAGAATCCGGGGCCGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACCGGGGACAAGAAGAAGGAGGAAGCA CCTTCTGGCAAAATGCAGGGGACCCTTGAAGATCAAATCATCAGTGCCAACCCTCTGCTGGAGGCCTTTG GCAATGCCAAGACTGTGAGGAACGACAACTCCTCCCGCTTCGGTAAATTCATCAGAATCCATTTCGGTGC CACAGGCAAACTGGCCTCCGCAGATATCGAAACCTATCTGCTAGAGAAGTCCAGAGTCACCTTCCAGCTC AAGGCTGAAAGAAGCTATCACATCTTCTACCAAGTCATGTCCAATAAGAAACCAGAGCTTATCGAGATGC TCCTGATCACCACCAACCCATATGACTTTGCTTACGTCAGTCAAGGTGAAATCACAGTGCCCAGCATTGA TGACCAGGAAGAGTTGATGGCCACAGACACTGCCGTGGACATCCTGGGGTTCACTGCAGATGAAAAAGTG GCCATTTACAAGCTCACTGGTGCTGTGATGCACTATGGGAACATGAAATTCAAGCAAAAGCAAAGGGAAG AGCAGGCTGAGCCAGACGGCACTGAAGTGGCTGACAAGGCTGCCTATCTGACAAGTCTGAACTCTGCTGA CCTGCTCAAAGCCCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTACGTCACCAAAGGCCAGACAGTG CAGCAGGTGTACAACTCTGTGGGCGCCCTGGCCAAGGCCATGTACGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGACTTCAACACCTTGGAGCAGCTGTGTATCAACTTCACCAACGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATTGAGTGGGAGTTCA TCGACTTCGGGATGGACTTGGCAGCCTGCATTGAGCTCATTGAGAAGCCGATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAACTGTATGAGCAGCATCTT GGGAAGTCCAACAACTTCCAGAAGCCCAAACCTGCCAAAGGCAAGGCCGAGGCCCACTTCTCCCTGGTGC ACTATGCAGGCACCGTGGACTACAACATTATTGGGTGGCTGGACAAGAACAAGGACCCCCTGAATGAGAC CGTGGTGGGGCTGTACCAGAAGTCCGGGTTGAAGACTCTGGCTTTCCTATTTTCTGGGGGACAAGCTGCT GAAGCAGAGGGTGGCGGCGGAAAGAAAGGTGGCAAGAAGAAGGGTTCTTCTTTCCAGACCGTGTCAGCTC TTTTCAGGGAGAATTTAAATAAGCTGATGACCAACTTGAAGAGCACCCACCCCCACTTTGTCCGATGCCT CATTCCCAATGAGACTAAGACTCCTGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTGAGGTGCAAC GGGGTGCTGGAAGGCATCCGCATCTGCAGGAAGGGGTTCCCCAGCAGGATCCTCTACGCAGACTTCAAGC AGAGATACAAAGTTCTCAATGCAAGTGCTATCCCGGAAGGTCAGTTCATTGATAGCAAGAAGGCCTCTGA GAAGCTTCTAGGTTCCATTGACATCGACCATACCCAGTACAAATTCGGTCACACGAAGGTTTTCTTTAAA GCTGGCCTGCTGGGAACTCTAGAGGAGATGCGAGATGAAAAGCTGGCTCAGCTCATCACGCGCACTCAAG CCGTGTGCAGAGGGTACCTGATGAGGGTGGAGTTCAGAAAGATGATGGAGAGGAGAGAGTCCATCTTCTG CATCCAGTACAACGTCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATC AAACCCCTGCTGAAGAGTGCAGAGACTGAGAAGGAGATGGCCACCATGAAGGAAGATTTTGAGAAGGCCA AGGAGGATCTGGCAAAGTCAGAGGCAAAAAGGAAAGAACTAGAAGAAAAGATGGTGGCTCTGATGCAAGA GAAAAATGATCTGCAGCTCCAGGTTCAAGCTGAAGCAGATGGCTTAGCTGATGCAGAGGAAAGATGCGAC CAGCTGATCAAAACTAAAATCCAACTGGAGGCCAAAATCAAAGAGTTGACTGAGAGGGCTGAGGATGAGG AAGAGATCAATGCCGAGCTCACCGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTGAAGAAAGA CATCGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAGGTGAAA AACCTCACAGAAGAGATGGCAGGCTTGGATGAAAATATTGTTAAGCTGACCAAGGAGAAGAAGGCCCTGC AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGC CAAAACCAAGCTCGAACAGCAAGTGGATGACCTTGAAGGATCACTGGAACAGGAAAAGAAGCTGAGGATG GACTTAGAAAGAGCCAAGAGGAAACTGGAGGGGGACCTGAAACTGGCCCAAGAGTCCACAATGGACATAG AAAATGACAAACAGCAGCTCGATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAACCTGCAGAGCAA GATTGAGGATGAACAGGCCCTCGGCATGCAGCTGCAGAAGAAGATCAAAGAGTTGCAGGCCCGCATTGAG GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCT CCCGGGAACTGGAGGAGATCAGCGAGCGGCTGGAAGAAGCCGGCGGGGCCACTTCAGCCCAGATCGAGAT GAACAAGAAGAGAGAGGCCGAGTTCCAGAAAATGCGCAGGGACTTGGAGGAGGCCACCCTGCAGCACGAA GCCACAGCAGCCGCTCTTCGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGATCGACAACC TGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGTGAGCTGAAGATGGAGATCGACGACCTCGCTAG CAACATGGAGACTGTCTCCAAAGCCAAGGGGAACCTTGAGAAGATGTGCCGCACCCTGGAGGACCAACTG AGTGAAGTGAAAACCAAGGAGGAGGAGCAGCAGCGCCTGATCAATGAGCTGTCAGCCCAGAAGGCACGCT TACACACAGAGTCAGGTGAGTTTTCCCGACAGCTGGATGAGAAAGATGCTATGGTTTCTCAGCTCTCCCG AGGCAAACAAGCGTTTACACAACAGATTGAGGAGCTAAAGAGGCAGCTAGAAGAAGAGTCCAAGGCCAAG AACGCGCTGGCCCACGCCCTGCAGTCCGCTCGCCACGACTGTGACCTTCTGCGGGAGCAGTATGAGGAGG AGCAGGAAGCCAAGGCCGAGCTGCAGAGGGCCATGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGAC GAAATATGAGACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGT CTGCAGGATGCTGAGGAGCACGTAGAAGCTGTGAATTCCAAGTGTGCTTCTCTTGAAAAGACGAAGCAGC GGCTGCAGAATGAGGTGGAGGACCTCATGATTGACGTGGAGAGGTCCAACGCTGCCTGCGCTGCGCTTGA TAAGAAGCAGAGGAACTTTGACAAGGTCCTGGCAGAATGGAAACAGAAGTATGAGGAAACTCAGGCTGAG CTCGAAGCCTCCCAGAAGGAGTCCCGTTCTCTCAGCACGGAGCTGTTCAAGGTGAAGAACGCCTATGAGG AGTCTCTGGATCAACTGGAGACGCTAAAGAGAGAGAACAAGAATCTACAGCAGGAGATTTCTGACCTGAC CGAGCAGATCGCAGAGGGAGGGAAGCATATCCACGAGCTGGAGAAAATCAAGAAGCAAATTGACCAGGAA AAGAGTGAATTACAGGCGTCTCTGGAGGAAGCGGAGGCCTCCCTTGAGCATGAAGAAGGTAAAATCCTAC GCATCCAGCTGGAGTTGAACCAGGTGAAATCCGAGATTGACCGCAAAATTGCTGAAAAAGACGAAGAAAT CGATCAGCTGAAGAGAAACCACCTCAGAGTAGTGGAGTCCATGCAAAGCACACTGGACGCTGAGATCCGC AGCAGAAACGATGCTCTGAGGATTAAGAAGAAGATGGAGGGCGACCTCAATGAAATGGAAATCCAGTTGA ACCATGCCAACCGCCAGGCTGCAGAGGCCATCAGGAACCTTAGGAACACACAGGGAATGCTCAAGGACAC GCAGCTGCACCTGGACGATGCTCTCAGAGGCCAGGATGACTTGAAAGAGCAGCTGGCCATGGTTGAGCGC AGGGCAAACCTGATGCAGGCTGAGATCGAGGAACTCAGGGCATCCCTGGAACAGACAGAGAGGAGCAGGA GAGTGGCTGAGCAAGAGCTACTGGATGCCAGTGAGCGTGTGCAGCTCCTGCACACCCAGAACACCAGCCT CATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAGATCCAGGGAGAGATGGAGGACATCGTCCAG GAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAAGCCATCACTGACGCCGCCATGATGGCGGAGGAGCTGA AGAAGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACGGTGAAGGACCT GCAGCACCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAG GCCAGAGTGAGGGAGCTTGAAAACGAGGTGGAAAATGAACAGAAGCGCAACATTGAAGCTGTCAAGGGTC TTCGCAAGCATGAGAGAAGAGTAAAGGAACTCACCTACCAGACCGAGGAGGACCGCAAGAACGTGCTGAG GCTGCAGGACCTAGTGGATAAATTACAGACTAAAGTGAAAGCCTACAAGAGACAAGCAGAGGAGGCTGAG GAACAATCCAACGTCAACCTGGCCAAGTTCCGCAAGATCCAGCACGAGCTGGAGGAAGCCGAGGAGCGGG CCGACATCGCCGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGAGAGGTTCACACCAAAGTCATAAG CGAAGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019325
Insert Size	5820 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019325.1, NP_062198.1
RefSeq Size	6088 bp
RefSeq ORF	5820 bp
Locus ID	360543
UniProt ID	Q29RW1
Gene Summary	fast myosin isoform expressed in skeletal muscle [RGD, Jun 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.