Nf1 (NM_012609) Rat Untagged Clone

CAT#: RN206616

Nf1 (untagged ORF) - Rat neurofibromin 1 (Nf1), (10 ug)



  "NM_012609" in other vectors (1)

CNY 54,340.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN206616 representing NM_012609
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCACACAGGCCGGTGGAATGGGTCCAGGCCGTGGTCAGCCGCTTCGACGAGCAGCTTCCAATAA
AAACAGGACAGCAGAACACACATACCAAAGTCAGCACGGAGCACAACAAGGAGTGTCTGATCAACATTTC
CAAGTACAAGTTTTCTTTGGTCATCAGTGGACTCACCACCATTCTAAAGAATGTTAACAATATGAGGATA
TTTGGAGAAGCTGCTGAAAAAAATTTGTATCTCTCTCAGTTGATCATATTGGATACACTGGAAAAATGTC
TTGCTGGGCAACCAAAGGACACAATGAGATTAGATGAAACAATGCTGGTCAAACAGTTGCTACCAGAAAT
CTGCCATTTTCTTCACACCTGCCGTGAAGGAAACCAACATGCAGCCGAACTTCGGAATTCTGCTTCTGGG
GTTTTATTTTCTCTCAGCTGCAACAACTTCAATGCAGTCTTTAGTCGAATTTCTACAAGGTTACAGGAGT
TGACTGTCTGTTCAGAAGACAATGTTGATGTTCATGATATAGAATTGTTACAGTATATCAATGTGGATTG
TGCAAAATTAAAACGACTCCTGAAGGAAACAGCATTTAAATTTAAAGCCCTGAAGAAGGTGGCACAATTG
GCAGTTATAAATAGCCTAGAAAAGGCTTTTTGGAACTGGGTGGAAAATTATCCAGATGAATTTACAAAGC
TATACCAAATTCCACAGACAGATATGGCTGAGTGTGCAGGAAAGCTGTTTGACTTGGTGGATGGTTTTGC
AGAAAGCACCAAACGTAAAGCAGCAGTTTGGCCACTACAGATCATTCTCCTCATTCTGTGTCCAGAAATA
ATCCAGGACATATCCAGGGATGTGGTCGATGAGAACAACACAAACAAGAAACTGTTTCTGGACAGTTTAC
GGAAAGCACTTGCTGGCCATGGCGGAAGCAGACAGCTGACTGAAAGTGCTGCCATCGCTTGTGTCAAGTT
GTGTAAAGCAAGTACTTACATCAACTGGGAAGACAACTCTGTCATTTTCCTACTGGTTCAGTCCATGGTG
GTTGATCTGAAGAACCTGCTTTTTAATCCAAGTAAACCATTCTCTAGAGGCAGTCAGCCTGCAGATGTGG
ACCTCATGATTGACTGTCTTGTCTCTTGCTTTCGGATCAGCCCTCACAACAACCAACACTTTAAGATCTG
TCTGGCTCAGAATTCACCTTCTACATTTCACTATGTGTTGGTAAACTCACTCCACCGCATTATCACCAAT
TCTGCATGGGATTGGTGGCCTAAGATTGACGCTGTATATTGTCACTCAGTTGAACTTCGAAATATGTTTG
GTGAAACCCTTCATAAAGCAGTGCAAGGCTGTGGAGCACACCCAGCATTACGGATGGCACCAAGTCTTAC
CTTTAAGGAAAAGGTAACAAGCCTTAAGTTTAAAGAAAAGCCTACGGACCTAGAGGCAAGGAGCTACAAG
TATCTTCTTTTGTCTATGGTGAAACTGATTCATGCAGACCCAAAGCTTTTGCTTTGTAATCCAAGAAAAC
AGGGCCCTGAAACTCAAGGTAGTACAGCAGAACTGATTACAGGGCTCGTACAGCTGGTTCCTCAGTCACA
CATGCCAGAGGTCGCTCAGGAGGCTATGGAGGCTTTGTTGGTTCTTCATCAGTTAGACAGCATTGATCTG
TGGAATCCTGATGCTCCTGTAGAAACGTTTTGGGAAATTAGTTCACAAATGCTTTTTTACATCTGCAAGA
AATTAACTAGTCATCAAATGCTTAGTAGCACAGAAATTCTCAAGTGGTTACGGGAAATTCTGATCTGCAG
GAATAAATTTCTTCTTAAAAATAAGCAGGCAGATAGAAGTTCCTGTCATTCTCTCTACCTTTATGGAGTA
GGATGTGACCTGCCTGCTAGTGGAAATGTCACTCAGATGTCAGTTGATCATGAGGAGTCACTACGTACCT
GTGCTCCTGGAGCCTCTCTGCGGAAAGGAAGAGGGAATTCCTCCATGGATAGCACAGCAGGGTGCAGTGG
AACCCCACCTATATGCCGACAAGCCCAGACCAAGCTAGAAGTGGCCTTGTACATGTTTCTGTGGAGTCCT
GACACGGAAGTTGTTCTGGTTGCTATGTCCTGTTTCCGCCACCTCTGTGAAGAAGCAGACATCCGATGTG
GTGTGGATGAAGTGTCAGTACACAACTTCTTGCCCAACTACAACACATTCATGGAATTTGCCTCAGTCAG
CAATATGTTGTCAACAGGAAGAGCGGCACTTCAGAAAAGAGTGATGGCCCTGCTAAGGCGCATTGAGCAC
CCTACTGCAGGAAACACTGAGGCCTGGGAAGATACACATGCAAAGTGGGAACAGGCTACAAAACTAATCC
TTAACTACCCAAAGGCCAAAATGGAAGATGGCCAGGCTGCAGAAAGTCTTCATAAGACCATTGTTAAGAG
ACGGATGTCCCATGTCAGTGGAGGAGGATCCATAGACTTGTCTGACACAGACTCCCTGCAGGAATGGATC
AACATGACTGGCTTCCTCTGTGCCCTTGGTGGGGTGTGCCTGCAACAGAGAAGCAGCTCTGGCCTAGCAA
CATACAGCCCACCTATGGGCCCTGTCAGTGAACGCAAAGGGTCTATGATTTCTGTAATGTCTTCTGAAGG
GAATGTTGATTCACCTGTCAGCAGATTTATGGACCGGCTTCTGTCCTTGATGGTGTGTAACCATGAGAAA
GTGGGGCTTCAGATACGGACCAATGTTAAGGACCTGGTGGGTCTGGAGCTGAGTCCTGCTCTGTATCCAA
TGCTGTTCAACAAACTGAAGAGTGCCATCAGCAAGTTTTTTGACTCTCAAGGACAGGTATTATTAACTGA
CAGCAATACTCAGTTTGTAGAGCAAACCATAGCTATAATGAAGAACCTACTGGATAATCATACTGAAGGC
AGCTCTGAGCATCTGGGACAAGCTAGCATTGAGACAATGATGCTAAATCTGGTCAGATATGTTCGTGTTC
TTGGGAATATGGTTCATGCAATTCAAATAAAAACGAAATTGTGTCAGTTAGTTGAAGTCATGATGGCAAG
AAGAGACGACCTCTCGTTTTGTCAAGAGATGAAATTTCGGAATAAGATGGTGGAGTACCTAACAGACTGG
GTTATGGGCACTTCAAACCAAGCCGCAGATGATGATGTAAAGTGTCTCACAAGAGATCTAGACCAAGCAA
GCATGGAGGCAGTAGTCTCGCTCCTCGCTGGTCTTCCACTGCAGCCTGAAGAAGGAGACGGAGTGGAGCT
GATGGAGGCCAAGTCACAGCTATTCCTTAAGTACTTCACATTATTTATGAACCTTTTGAATGACTGTAGT
GAAGTTGAAGATGAAAATGCACAAACAGGTGGCAGGAAACGTGGCATGTCTCGGAGGCTGGCATCCCTGA
GGCACTGTACAGTGCTTGCAATGTCAAACTTACTCAATGCTAATGTGGACAGTGGACTCATGCACTCCAT
AGGCTTAGGTTATCATAAAGATCTTCAGACAAGAGCTACATTTATGGAAGTTCTGACAAAAATCCTTCAA
CAAGGAACAGAATTTGATACACTTGCTGAAACCGTGTTGGCAGATCGGTTCGAGAGACTTGTGGAACTGG
TCACAATGATGGGAGACCAGGGAGAGCTCCCTATAGCTATGGCTCTGGCCAATGTGGTCCCTTGTTCTCA
GTGGGATGAGCTGGCTCGAGTTCTGGTCACGCTGTTTGATTCTCGGCATTTGCTCTACCAGCTGCTCTGG
AACATGTTTTCTAAGGAGGTAGAGTTGGCAGACTCCATGCAGACTCTCTTTCGAGGCAACAGCTTGGCCA
GTAAGATAATGACGTTCTGCTTCAAGGTGTACGGTGCTACTTACCTACAAAAGCTCTTGGACCCCTTGTT
ACGAATCATTATCACATCCTCCGATTGGCAGCATGTTAGCTTTGAAGTGGATCCTACCAGGTTAGAGCCC
TCTGAGAGCCTTGAGGAGAACCAGAGGAACCTCCTTCAGATGACAGAGAAGTTCTTCCATGCCATCATCA
GTTCTTCCTCAGAGTTCCCCTCGCAGCTTCGAAGTGTCTGCCATTGTTTGTACCAGGTGGTTAGCCAGCG
CTTCCCTCAGAACAGCATCGGTGCCGTCGGAAGTGCCATGTTCCTGAGGTTCATCAACCCTGCCATTGTC
TCACCGTATGAAGCAGGGATTTTAGATAAAAAGCCACCACCTAGAATTGAAAGGGGCTTGAAGTTAATGT
CAAAGGTCCTTCAGAGCATTGCCAATCATGTACTGTTCACAAAGGAAGAGCACATGAGGCCTTTTAATGA
TTTTGTGAAAAGCAACTTCGACTTGGCACGGAGGTTTTTCTTAGATATAGCATCAGATTGTCCCACAAGT
GACGCAGTAAACCATAGTCTTTCCTTCATCAGTGATGGCAATGTGCTCGCTTTACATCGGCTGCTTTGGA
ATAATCAGGAGAAAATTGGCCAGTATCTTTCCAGCAACAGGGATCATAAAGCTGTTGGAAGACGACCTTT
CGATAAGATGGCCACACTTCTTGCATATCTGGGTCCTCCGGAGCACAAGCCTGTGGCAGATACACACTGG
TCCAGCCTTAACCTTACCAGTTCAAAGTTTGAGGAATTTATGACCAGGCATCAGGTTCATGAGAAGGAAG
AGTTCAAGGCCTTGAAAACATTAAGCATCTTCTACCAAGCTGGCACATCCAAAGCTGGGAATCCTATTTT
CTATTATGTTGCACGGAGGTTCAAAACTGGCCAAATCAATGGGGATTTGCTGATATACCATGTCTTGCTG
ACTTTAAAGCCATATTATGCAAAGCCATATGAAATTGTAGTGGACCTTACCCATACTGGGCCTAGCAATC
GCTTTAAAACAGACTTCCTCTCTAAGTGGTTTGTTGTTTTTCCTGGCTTTGCTTATGACAACGTGTCTGC
AGTCTATATCTATAACTGTAACTCCTGGGTCAGGGAGTACACCAAATACCATGAGCGGCTGCTGACTGGC
CTCAAGGGCAGCAAAAGGCTCATTTTCATCGACTGTCCTGGGAAGCTGGCTGAGCACATAGAGCACGAGC
AACAGAAGCTCCCTGCTGCTACCTTGGCACTGGAAGAGGATCTAAAGGTTTTCCACAATGCGCTCAAGCT
AGCTCACAAGGACACCAAAGTTTCTATTAAGGTTGGTTCTACTGCTGTTCAGGTAACCTCAGCAGAAAGA
ACAAAAGTATTGGGGCAATCCGTCTTTCTAAATGATATCTACTATGCTTCTGAAATCGAAGAGATCTGCC
TAGTTGATGAGAACCAGTTCACTTTAACCATTGCAAACCAAGGCACACCGCTGACCTTTATGCACCAAGA
GTGTGAAGCCATTGTTCAGTCTATCATTCATATCAGGACCCGATGGGAACTGTCACAGCCTGACTCCATC
CCTCAGCATACCAAGATTCGACCAAAAGATGTCCCTGGAACACTGCTTAATATCGCATTACTGAATTTGG
GCAGTTCAGACCCTAGTTTACGGTCTGCTGCCTATAACCTCCTGTGTGCCTTAACTTGTACCTTTAATTT
AAAGATCGAAGGTCAGTTACTAGAGACATCAGGGTTATGCATCCCTGCCAACAACACCCTCTTCATTGTC
TCTATCAGTAAAACGCTTGCAGCCAACGAGCCACACCTCACCTTAGAGTTTTTGGAAGAGTGTATTTCTG
GATTTAGCAAATCTAGTATTGAATTGAAACACCTTTGTTTGGAATATATGACCCCATGGCTGTCAAACCT
AGTCCGTTTTTGTAAGCATAACGATGACGCCAAACGACAAAGGGTTACTGCCATCCTTGATAAACTCATA
ACAATGACCATAAATGAAAAGCAGATGTACCCTTCTATTCAAGCAAAAATATGGGGGAGCCTTGGGCAGA
TCACAGACCTGCTAGATGTTGTGCTTGACAGTTTCATCAAAACCAGTGCTACCGGAGGGTTGGGGTCTAT
CAAAGCTGAGGTGATGGCAGACACAGCTGTGGCTTTAGCTTCTGGAAATGTGAAACTGGTGTCAAGTAAG
GTTATTGGAAGGATGTGTAAAATAATTGACAAGACTTGCTTATCTCCAACTCCAACTTTAGAACAGCATC
TTATGTGGGACGACATTGCCATTTTAGCCCGCTATATGCTGATGCTGTCCTTCAACAACTCCCTGGATGT
GGCCGCTCATCTCCCCTATCTCTTCCATGTTGTCACCTTCTTAGTAGCCACAGGTCCTTTGTCCCTTCGC
GCTTCCACCCACGGATTGGTCATTAATATCATTCACTCTCTGTGCACTTGCTCACAGCTTCATTTTAGTG
AAGAGACCAAGCAAGTTTTGAGGCTCAGTCTGACAGAGTTCTCGTTACCCAAATTTTACTTACTGTTTGG
CATCAGCAAAGTCAAGTCAGCTGCTGTCATTGCCTTCCGTTCCAGCTACCGGGACCGGTCGTTCTCTCCT
GGCTCCTATGAAAGGGAGACTTTTGCTTTGACGTCCCTGGAAACAGTCACAGAAGCTTTGTTGGAGATCA
TGGAGGCATGTATGAGAGATATTCCAACATGCAAGTGGCTGGATCAGTGGACGGAACTGGCTCAAAGATT
TGCATTTCAGTACAACCCATCCCTGCAGCCAAGAGCTCTTGTGGTGTTTGGCTGTATTAGCAAACGTGTG
TCTCACGGGCAGATAAAGCAGATTATCCGAATCCTTAGCAAGGCACTTGAAAGTTGTTTAAAGGGACCTG
ACACTTACAACAGTCAAGTTCTGATAGAAGCCACAGTGATTGCACTGACCAAACTACAGCCACTTCTTAA
TAAGGACTCACCACTGCACAAAGCCCTCTTCTGGGTTGCTGTGGCTGTGCTGCAGCTAGATGAAGTCAAC
TTGTACTCAGCTGGCACTGCACTTCTGGAACAGAATCTGCATACCTTGGATAGTCTCCGGATATTCAATG
ACAAGAGTCCGGAAGAGGTATTTATGGCAATCCGGAATCCTCTGGAATGGCACTGCAAGCAAATGGATCA
CTTTGTTGGACTCAATTTCAACTCTAATTTTAACTTTGCACTAGTTGGACACCTCTTAAAAGGGTACAGA
CATCCTTCACCTGCCATTGTTGCAAGAACAGTCAGAATTTTGCATACACTACTAACTCTTGTTAACAAAC
ATAGAAATTGTGACAAATTTGAGGTGAATACACAGAGTGTGGCTTACTTAGCAGCTCTACTCACAGTGTC
TGAAGAGGTTCGAAGTCGCTGTAGCCTCAAGCATAGGAAGTCCCTTCTTCTTACTGATATCTCAATGGAA
AATGTTCCTATGGATACATATCCCATTCATCACGGTGATCCCAGCTCTAGGACACTAAAGGAGACTCAGC
CATGGTCCTCTCCCAGAGGTTCAGAAGGATACCTTGCAGCCACCTACCCAGCTGTAGGCCAAACTAGTCC
CCGAGCCAGGAAATCCATGAGCTTGGACATGGGACAGCCTTCTCAGGCCAACACAAAAAAATTGCTTGGA
ACAAGGAAAAGTTTTGATCACTTGATATCAGACACAAAGGCTCCTAAAAGACAAGAAATGGAATCAGGGA
TTACAACACCCCCAAAAATGAGAAGAGTGGCAGAGACCGACTATGAAATGGAAACCCAAAGGATTTCCTC
CTCGCAGCAGCACCCACATTTACGGAAAGTCTCAGTGTCTGAATCTAATGTTCTATTGGACGAAGAAGTA
CTTACTGACCCAAAGATCCAAGCACTGCTTCTTACTGTTCTGGCTACACTGGTAAAATATACCACAGATG
AATTTGATCAGCGAATTCTTTATGAATATCTAGCAGAGGCCAGTGTTGTGTTTCCCAAAGTGTTTCCTCT
TGTGCACAATTTGTTGGACTCTAAGATCAATACCCTCTTGTCTTTGTGCCAAGATCCAAATTTGTTAAAT
CCGATCCATGGAATCGTGCAGAGTGTGGTGTACCATGAGGAATCCCCTCCACAGTACCAAACATCATACC
TGCAAAGTTTCGGTTTTAATGGCTTATGGCGGTTTGCAGGACCCTTTTCAAAGCAAACACAAATCCCAGA
CTATGCTGAGCTCATTGTTAAATTTCTTGATGCCTTGATTGACACGTACTTGCCTGGAATTGATGAAGAA
ACCAGTGAGGAGTCCCTCCTGACACCCACATCTCCTTACCCTCCTGCACTGCAGAGCCAGCTTAGTATCA
CTGCCAACCTTAACCTCTCTAATTCTATGACCTCACTTGCAACTTCCCAGCATTCCCCAGGAATCGACAA
GGAGAACGTTGAACTCTCCCCCACCACTGGCCACTGTAACAGTGGACGGACTCGCCATGGATCCGCAAGC
CAAGTGCAGAAGCAAAGGAGTGCTGGCAGTTTCAAACGTAATAGCATTAAGAAGATCGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_012609
Insert Size 8463 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012609.1, NP_036741.1
RefSeq Size 9132 bp
RefSeq ORF 8463 bp
Locus ID 24592
UniProt ID P97526
Gene Summary mutations in gene are associated with neurofibromatosis type 1 [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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