Abcc9 (NM_013040) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sur2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206569 representing NM_013040 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTTTCCTTCTGTGGTAACAACATCTCCTCCTACAACATCTACCATGGTGTTCTCCAAAACCCCT GCTTTGTGGACGCACTCAACCTGGTCCCTCACGTCTTCCTGCTGTTTATCACCTTTCCGATCCTGTTCAT TGGATGGGGGAGCCAAAGCTCAAAAGTGCAAATTCATCACAACACATGGCTTCATTTTCCTGGACACAAC CTGAGATGGATTCTGACGTTTGCACTCCTGTTTGTGCATGTCTGCGAGATAGCAGAAGGCATTGTTTCAG ACTCGCAACGGGCGTCCAGGCATCTCCACCTTTTCATGCCGGCTGTGATGGGATTTGTCGCCACCACCAC ATCCATTGTGTATTACCACAACATTGAAACATCAAACTTCCCTAAATTACTTTTAGCTTTATTCCTGTAC TGGGTCATGGCCTTCATTACAAAGACAATAAAGTTGGTCAAATACTGGCAGTTGGGGTGGGGAATGTCAG ACCTGCGCTTCTGCATCACGGGAGTGATGGTCATCTTGAATGGACTGCTGATGGCTGTGGAGATCAATGT CATCCGGGTCCGAAGATATGTTTTCTTCATGAATCCTCAGAAAGTGAAGCCTCCAGAGGACCTCCAGGAC CTGGGCGTGAGGTTCCTCCAGCCGTTTGTGAATTTGCTGTCCAAAGCTACTTACTGGTGGATGAACACGC TTATCATATCAGCTCACAGGAAACCTATTGATCTGAAGGCGATCGGAAAGTTGCCGATAGCAATGAGGGC GGTGACGAATTATGTTTGCTTGAAGGAGGCCTACGAAGAGCAGAAGAAAAAGGCTGCGGATCATCCGAAT CGGACTCCCTCCATATGGCTGGCCATGTACAGGGCTTTTGGGAGACCGATCCTGCTGAGCAGCACGTTCC GCTACTTGGCTGACTTGCTGGGCTTTGCCGGCCCTCTTTGTATTTCCGGAATCGTCCAGCGTGTGAATGA ACCGAAGAATAACACGACGAGATTTTCAGAGACACTCTCATCAAAGGAGTTTCTGGAAAATGCCCATGTG CTGGCTGTCCTGCTCTTCCTGGCCCTCATCCTGCAAAGAACATTTTTGCAGGCTTCGTACTATGTGACCA TAGAGACCGGCATCAACCTGCGTGGGGCTCTGCTGGCTATGATCTACAACAAAATCCTTCGACTGTCTAC TTCTAACCTATCCATGGGCGAGATGACCCTGGGACAGATCAACAACTTGGTTGCCATAGAAACCAATCAG CTCATGTGGTTCTTGTTCCTGTGTCCCAATCTGTGGGCCATGCCCGTTCAGATCATAATGGGGGTGATCC TGCTCTATAATCTGCTTGGGTCAAGCGCACTGGTAGGCGCGGCGGTCATCGTGCTCCTCGCACCGATTCA GTACTTCATCGCCACGAAGCTGGCGGAGGCTCAGAAGAGCACTCTGGATTATTCCACCGAGAGGCTGAAG AAGACAAACGAGATACTGAAAGGCATCAAGCTCCTTAAGCTGTATGCCTGGGAGCACATTTTCTGCAAGA GCGTGGAAGAGACTAGAATGAAGGAGCTCTCCAGCCTCAAAACCTTCGCGCTCTACACGTCGCTTTCCAT CTTCATGAACGCAGCCATTCCCATCGCAGCCGTTCTTGCAACATTTGTGACCCATGCCTATGCCAGTGGC AACAACCTGAAACCCGCAGAGGCCTTTGCCTCTCTGTCTCTCTTCCACATCCTCGTCACACCGCTCTTCC TGCTGTCCACGGTGGTCAGATTCGCAGTGAAAGCCATCATCAGCGTTCAGAAGCTGAATGAGTTTCTCTT GAGCGATGAGATTGGCGAGGACAGCTGGAGGACTGGGGAGGGGACGCTGCCTTTTGAGTCCTGTAAGAAG CACACCGGAGTGCAATCAAAACCGATAAACAGGAAGCAGCCTGGAAGGTACCACCTGGACAACTACGAGC AGGCGCGGCGTCTTCGGCCTGCTGAGACTGAAGATGTTGCCATAAAGGTGACGAACGGATACTTCTCATG GGGCAGTGGTTTAGCCACATTATCCAATATTGACATTCGAATTCCAACAGGTCAGCTAACCATGATTGTG GGTCAGGTGGGTTGTGGCAAATCGTCTCTCCTCCTTGCCATCCTTGGTGAGATGCAGACCCTGGAAGGAA AAGTTTACTGGAACAATGTAAATGAATCTGAGCCTTCTTTTGAAGCAACCCGAAGCAGAAGCAGGTACTC TGTGGCTTATGCTGCCCAGAAGCCTTGGCTCCTCAATGCTACGGTCGAGGAAAACATCACTTTTGGAAGT TCTTTCAACAGACAGAGGTACAAGGCTGTCACCGATGCCTGCTCTCTGCAGCCAGACATTGATTTGTTAC CCTTTGGAGACCAAACTGAAATTGGAGAGAGGGGTATCAACCTGAGTGGGGGTCAGAGGCAGAGAATCTG CGTGGCCCGGGCACTCTACCAGAACACCAATATTGTCTTCTTGGACGATCCATTCTCCGCTCTGGACATC CACCTGAGCGACCACTTGATGCAGGAAGGGATCCTGAAGTTTCTCCAGGACGACAAGAGGACGGTCGTCC TTGTGACTCACAAACTACAGTACCTGACGCACGCGGACTGGATCATAGCCATGAAGGATGGGAGTGTGTT AAGGGAAGGGACTTTGAAAGACATCCAGACCAAAGACGTGGAGCTCTATGAACACTGGAAAACCCTCATG AATCGGCAAGATCAGGAATTAGAAAAGGACATGGAAGCCGACCAAACAACGCTGGAGAGGAAGACTCTCC GAAGAGCTATGTACTCAAGGGAGGCCAAAGCACAGATGGAGGATGAAGATGAAGAGGAGGAGGAGGAGGA AGATGAGGACGACAACATGTCAACTGTAATGAGGCTCAGGACGAAGATGCCCTGGAAGACCTGTTGGTGG TACCTCACTTCAGGAGGGTTTTTCCTGCTCTTCCTCATGATCTTCTCTAAGCTTTTGAAGCACTCTGTGA TCGTGGCCATCGACTACTGGCTAGCTACGTGGACCTCCGAGTACAGTATAAACGACCCAGGGAAAGCTGA CCAGACCTTCTATGTGGCTGGGTTCAGCATCCTCTGTGGAGCGGGCATTTTCCTTTGCCTCGTCACCTCC CTCACTGTAGAATGGATGGGTCTCACCGCCGCCAAGAACCTCCACCACAATCTCCTCAATAAGATAATTC TGGGCCCAATAAGGTTCTTTGATACCACGCCCCTGGGACTGATCCTCAATCGGTTTTCTGCTGATACCAA CATCATCGACCAACATATCCCTCCGACCTTGGAGTCGCTGACCCGCTCTACCCTGCTCTGCCTGTCGGCT ATTGGGATGATCTCCTATGCTACACCCGTGTTTCTCATCGCTCTTGCGCCCCTGGGCGTCGCCTTTTATT TCATCCAGAAATACTTCCGGGTTGCCTCTAAGGATCTCCAGGAACTCGATGACAGCACCCAGCTCCCCCT GCTTTGTCACTTCTCAGAAACAGCTGAAGGGCTTACCACTATCCGGGCCTTCAGGCATGAAACCAGATTC AAGCAACGCATGCTGGAGCTGACAGACACAAACAACATTGCCTACTTATTTCTCTCCGCAGCCAACAGAT GGCTGGAGGTCAGGACGGACTACCTGGGAGCTTGCATTGTTCTGACGGCCTCCATTGCATCCATCAGTGG CTCTTCCAACTCTGGACTAGTGGGCTTGGGCCTTCTGTATGCCCTCACGATAACCAATTACCTGAATTGG GTTGTAAGGAACTTGGCCGACCTCGAAGTCCAGATGGGCGCAGTGAAGAAAGTGAACAGTTTCTTAACTA TGGAGTCTGAGAACTATGAAGGCACCATGGATCCTTCTCAAGTCCCAGAGCATTGGCCACAGGAAGGTGA GATCAAGATTCACGATCTATGCGTCAGATATGAAAATAACCTGAAGCCCGTTCTGAAACATGTCAAGGCT TACATCAAGCCTGGGCAGAAGGTGGGCATCTGTGGTCGCACCGGTAGTGGGAAGTCCTCTCTATCTCTGG CTTTCTTCAGAATGGTCGACATATTTGATGGAAAGATAGTCATTGATGGAATAGACATTTCCAAGCTGCC CTTGCACACGCTCCGCTCTAGACTGTCCATCATTCTCCAGGACCCAATCCTGTTCAGCGGCTCTATCAGA TTTAACTTGGATCCTGAATGCAAGTGCACAGACGACAGGCTCTGGGAGGCTCTGGAAATTGCTCAGTTGA AGAATATGGTCAAATCTCTGCCAGGAGGCCTAGACGCCACTGTCACCGAAGGTGGTGAGAACTTCAGCGT TGGACAGAGACAGCTGTTCTGCCTGGCCAGGGCCTTTGTTCGAAAGAGCAGCATACTCATTATGGATGAG GCCACGGCCTCCATCGACATGGCCACGGAAAACATTTTGCAGAAAGTAGTCATGACAGCCTTTGCGGATC GCACGGTTGTAACCATAGCTCATCGGGTTCACACCATTCTGACTGCTGACCTGGTCATTGTGATGAAGAG AGGAAACATTTTGGAATACGACACCCCGGAAAGCCTCTTGGCCCAGGAGGATGGAGTGTTCGCCTCGTTC GTTCGTGCGGACATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013040
Insert Size	4638 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013040.2, NP_037172.2
RefSeq Size	6628 bp
RefSeq ORF	4638 bp
Locus ID	25560
UniProt ID	Q63563
Gene Summary	ATP-sensitive potassium channel subunit that confers ATP sensitivity and acts as receptor for sulfonylurea; may play a role in mesangial cell contractility in response to calcium ion transients [RGD, Feb 2006]

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