Tjp1 (NM_001106266) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ZO-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206456 representing NM_001106266 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAAACAGCTATATGGGAACAGCACACAGTGACGCTTCACAGGGCTCCTGGGTTTGGATTTGGAA TTGCAATATCTGGTGGAAGAGATAATCCTCATTTTCAGAGTGGGGAAACCTCCATAGTGATTTCTGATGT GCTAAAAGGAGGGCCAGCTGAAGGACAGCTACAGGAAAATGACCGAGTCGCAATGGTTAACGGAGTTTCA ATGGATAATGTTGAACATGCTTTTGCTGTTCAGCAGCTAAGGAAAAGTGGGAAAAACGCAAAAATTACCA TCCGAAGAAAGAAGAAAGTTCAGATTCCTGTAAGTCACCCAGACCCTGACCCAGTGTCTGATAATGAAGA TGATAGCTATGACGAGGATGTGCACGATCCAAGAAGTGGCCGAGGTGCCCTAGCTAACAGAAGGGGTGAG AAGAGCTGGGCAAGGGATAGAAGCGCAAGCAGGGACCGGAGCCTGTCCCCTCGCTCAGACAGGCGATCAG TGGCCTCCAGTCAGCCCGCCAAACCCACCAAGGTCACATTGGTGAAGTCTCGGAAAAATGAAGAATATGG TCTTCGATTGGCCAGCCATATATTTGTAAAGGAAATTTCACAAGATAGTTTGGCAGCAAGAGATGGTAAC ATTCAAGAAGGGGATGTTGTCCTGAAGATAAATGGTACTGTGACAGAAAATATGTCACTGACAGATGCAA AAACATTGATAGAAAGGTCTAAAGGCAAGTTAAAAATGGTAGTGCAAAGAGATGAGCGGGCTACCTTATT GAATGTCCCTGATCTTTCAGATAGTATCCATTCTGCTAATGCCTCTGAAAGAGATGACATTTCAGAAATT CAGTCACTAGCATCAGACCATTCAGTTCGCTCCCATGACAGGCCTCCCCGCCGAAGCCAGTCACGATCTC CCGACCAACGTTCAGAGCCCTCTGATCATTCCACACAGTCTCCACAGCAGCCCAGCAATGGCAGTCTCCG GAGCAGAGAGGAAGAGCGCATGTCTAAACCCGGGGCTGTCTCAACTCCTGTAAAACATGTAGATGATCAT ACACCCAAAGCAGTGGAAGAAGTTACAGTTGAAAAACATGAGAAGCAGACACCCACTCTTCCAGAACCAA AACCTGTATATGCTCAAGTCGGACAGCCAGATGTGGATTTACCTGTCAGCCCTTCTGATGGTGTTCTGCC AAATTCAACACATGAAGATGGGATTCTTAGGCCCAGCATGAAACTGGTAAAATTCAGAAAAGGAGATAGT GTGGGTTTGCGACTAGCTGGTGGAAATGATGTCGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTG CAGCCAAAGAAGGCTTAGAGGAAGGTGATCAAATTCTCAGGGTGAACAATGTAGATTTCACAAATATCAT AAGAGAAGAAGCTGTTCTTTTCCTCCTCGACCTCCCTAAAGGTGAAGAAGTGACCATATTGGCTCAGAAG AAAAAGGACGTTTATCGCCGCATTGTAGAGTCAGATGTAGGAGACTCGTTCTATATTAGAACACATTTTG AATATGAGAAAGAATCTCCTTATGGACTCAGTTTTAACAAAGGAGAGGTGTTCCGGGTCGTGGACACCCT GTACAACGGGAAGCTGGGCTCCTGGCTGGCCATTCGAATTGGCAAAAATCACAAGGAGGTAGAGCGAGGC ATCGTTCCTAATAAGAACAGAGCCGAGCAGTTAGCCAGTGTACAGTACACACTTCCAAAGACAGCAGGTG GCGATCGGGCAGACTTCTGGAGGTTTCGAGGTCTTCGTAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGA GGACCTGTCAGCGCAGCCAGTTCAAACAAAGTTCCCAGCTTATGAAAGGGTTGTTCTTCGAGAAGCTGGA TTCCTAAGACCTGTAACCATCTTTGGACCGATTGCTGATGTTGCCAGAGAAAAGTTAGCAAGGGAGGAGC CAGACATTTATCAGATTGCAAAAAGTGAACCACGAGATGCTGGAACTGACCATCGGAGCTCGGGCATTAT TCGCCTTCATACAATAAAGCAAATCATAGATCAAGATAAACATGCTTTATTAGATGTCACACCAAATGCA GTCGATCGCCTTAATTATGCACAGTGGTATCCGATTGTTGTGTTCCTTAACCCTGACTCTAAGCAGGGTG TAAAAACAATGAGGATGAGGCTATGTCCAGAGTCTCGGAAAAGTGCCAGGAAGCTATATGAACGGTCTCA TAAACTTCGTAAAAATAATCACCATCTTTTTACAACCACAATTAACTTAAACTCAATGAATGATGGTTGG TATGGTGCCCTGAAAGAAGCGATTCAGCAGCAACAGAACCAGCTGGTGTGGGTCTCTGAGGGGAAGGCGG ATGGTGCTACAAGTGATGACCTTGATTTGCATGATGATCGTCTGTCCTACCTGTCAGCCCCAGGTAGTGA GTACTCAATGTATAGCACGGACAGTAGACACACTTCTGACTATGAAGACACAGATACAGAAGGCGGGGCC TACACTGATCAAGAACTAGATGAAACTCTTAATGATGAGGTGGGGACTCCACCGGAGTCTGCTATTACAC GGTCCTCTGAGCCTGTAAGAGAGGATTCCTCTGGAATGCATCATGAAAACCAAACATATCCTCCTTACTC ACCACAAGCGCAGCCACAAGCTATTCATAGAATAGACTCCCCTGGACTTAAGACAGCCTCTCAACAGAAA GCAGAAGCCTCATCTCCAGTCCCTTACCTTTCGCCTGAAACAAACCCAGCATCATCAGCCTCTGCAGTTA AGCATAATGTAAATTTAACTAATGTCAACCTGGAGGAGCCCACCCCAGCTCCTCCCACCTCGCACGTATC ACAAGCTGATTGTTTAGGAGCACCAAGTCCTGAGGCACCTCACACGATGCTCAGAGACGAAGGAGTGTCA TTGCCGTCGCATGTAGACCCAGCAAAGGTATATAGGAAGGAGCCATATCCTGAGGAAATGATGAGACAAA ACCATATTTTAAAACAGCCAGCTCTTGGTCATCCAGGGCAGAGGCTGGATAAAGAGCCAAATCCAGCCTA TGATCCCCAACTTCCATATGTAGAAAAACAAGCCAGCAGAGACCTTGAGCAGCCACCATACAGGTATGAG TCCTCAAGCTACACAGACCAGTTTTCTCGGAACTATGACCATCGCCTACGATTTGAAGACCGAGTCCCTA CCTATGAAGACCAGTGGTCATATTATGATGACAAACAGCCCTACCCAACTCGGCCCTTTGATACTCAGCA TCCTCGAGACTTGGACTCCAGACAGCATCCTGAAGAGGCTTCAGAACGAGGCTATTTCCAGCGTTTTGAA GAGCCCGCCCCTCTGCCATATGACAGTAGACCACGCTACGAGCAGCTGCCTCGAACCTCTACTCTCCGAC ATGAAGAACAGCCAACCAGTGGATATGAGGTGCACAACAGGTACAGGCCAGAAGCACAGCCCTATGCTCC AGCAGGTCCTAAGTCATCTGAGCCGAAGCAGTACTTTGACCAGTACCCACGAAGTTATGAGCAAGTACCA CCACCAGGATTTACCTCCAAAACAGGCCATTACGAGCCTCTCCATGGTGCTGCAGTTGTTCCTCCTCTGA TACCTTCCTCTCAACATAAGCCAGAAGTTCTGCCCTCAGCTACCAAGCCACAGCCTCCGCCCCCAGCCCT AACTGAGGAAGAGGAGGATCCAGCAATGAAACCACAGTCTGTGCTCACCAGGGTCAAAATGTTTGAAAAC AAAAGATCTGCGTCTCTGGAGAACAAGAAGGATGTGAATGACACTGCCAGCTTTAAGCCTCCAGAAGTAG CATCTAAACCTCCAAGTGCTTCTCTTGTTGGCCCTAAACCTGTTTCTCAGACTCAGTTTAGTGAGCATGA CAAAACACTCTACAGGCTCCCAGAGCCTCAGAAACCTCAAGCGAAGCCACCTGAAGATATTGTTCGATCA AATCATTACGATCCTGAAGAGGATGAAGAGTATTACCGGAAACAGCTCTCTTACTTTGACCGGAGAAGTT TCGAGAGCAAGCCTCCTGCACATATTCCTGCTGGCCATCACTCAGAGCCTGCCAAGCCAGTCCATTCTCA GAGTCAGCCGAATTTCTCTAGTTATTCTTCAAAGGGGAAACCCGAAACTGATGCTATGGATAGATCATTC AGTGAGAAACGTTATGATCCAACCCAGGCCATGCCTCCTCCTCCTCCGTTGCCCTCACAGTACAGCCAGC CAGTTCCGCCTCTGTCCAACTCTTCTCTCCACATACATTCCAAGGCCGCCCAGAGTGAAGGCAATTCCGT ATCGTTGGATTTTCAGAACTCATATATATCCAAACCAGACCCACCCCCATCTCAGAGCAAACCAGCAACT TTTAGACCACCAACCCGGGAGGACCCTCCTCAGACCTTCTATCCCCAGAAAAGTTTCCCAGACAAAGCTT CAGTTAATGGAGCTGAGCAGACTCAGAAAACCATCACTCCAGCATACAACCGATTCACACCAAAGCCGTA CACGAGCTCTGCCCGGCCATTTGAACGCAAGTTTGAAAGTCCAAAGTTCAACCATAATCTTCTGCCAAGT GAAACTGTACATAAACCTGAATTGTCTTCAAAACCTCCCCCTTCTCCAAAAACTCTCATGAAGGCTCATA GTTCCACACAGCCGCCTGAGTTTGACAGTGGAGTCGAGACTTTCTCTGTTCACACAGATAAGCCTAAATA TCAAATAAATAATATCAGCACCATGCCTAAAGCTGTTCCTGTGAGTCCTTCAGCTGTGGAAGAAGATGAA GATGAGGATGGTCATACTGTAGTGGCTACAGCCCGCGGCATTTTTAACAGCAATGGCGGTGTGTTGAGTT CCATAGAAACTGGTGTTAGTATAATTATCCCACAAGGAGCCATTCCTGAAGGAATTGAGCAAGAAATCTA TTTCAAAGTCTGCAGAGACAATAGCATCCTCCCACCTTTAGATAAAGAGAAAGGTGAAACTCTGCTGAGC CCCCTAGTGATGTGCGGGCCCCATGGCCTCAAGTTCCTGAAGCCTGTGGAGCTACGCTTGCCACACTGTG ACCCTAAAACTTGGCAAAACAAGTGTCTTCCTGGAGATCCGAATTACCTTGTTGGAGCCAACTGTGTTTC TGTCCTGATTGACCACTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106266
Insert Size	5202 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106266.1, NP_001099736.1
RefSeq Size	6614 bp
RefSeq ORF	5202 bp
Locus ID	292994

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