Insrr (NM_022212) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sirr
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206402 representing NM_022212 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCCTGCTCTGTGGCCCTGGGGAGTATACTTGCTTATGAGCCTGCTGTCCTTGGGATCTTGCC TGGACACACTGGAGGTGTGCCCCAGTCTTGACATCCGCTCGGAGGTGACAGAGCTCCGTCGGCTGGAGAA CTGCAGTGTGGTGGAGGGTCACCTACAGATCCTCCTCATGTTTGCCGCCACGGGAGAGGACTTCCGAGGC CTCAGCTTTCCTCGCCTCACCCAGGTGACTGACTACCTGCTGCTGTTCCGAGTCTATGGCCTAGAGAGCC TTCGAGACCTCTTCCCCAACCTTGCTGTGATCCGAGGTGCGCGCCTCTTCCTGGGCTATGCACTTATAAT CTTCGAGATGCCCCACTTGAGGGACATTGGGCTGCCGTCGCTTGGGGCCGTGCTGCGTGGGGCCGTTCGT GTGGAGAAGAACCAGGAACTTTGCCATCTCTCCACCATTGACTGGGGCCTGCTGCAGCCTGCACCTGGTG CCAACCACATTGTTGGGAACAAGCTGGGTGAGGAGTGTGCTGACGTGTGCCCTGGTGTGCTGGGGGCTGC TGGTGAGCCCTGTGTGAGAACCACCTTCGGTGGGCACACCGACTACAGGTGTTGGACCTCCAGCCACTGT CAGAGAGTGTGTCCCTGCCCCCGTGGGCTGGCCTGCACAGTAGGTGGCGAGTGCTGCCACAGTGAATGTC TCGGGGGCTGCAGTCAGCCTGAAGACCCTCGAGCCTGCGTAGCTTGCCGCCACCTCTACTTCCAAGGAGT CTGCCTCCCCACCTGCCCTCCGGGCACCTACCAGTATGAGTCTTGGCGGTGTGTCACTGCGGAGCTCTGT GGCCATCTGCGAGAGGTTCCTGGACATGCCACCGCCTTTGGCATCTACGAGGGCAGCTGCCTAGCTCAGT GCCCTCCAGGCTTCACCCGTAATGGCAGCAGCATTTTCTGCCATAAGTGTGAGGGCCTGTGCCCCAAAGA GTGCAAGGTTGGGACAAAGACCATCGACTCTGTCCAAGCCACACAGGACCTAGTGGGCTGCACCCATGTG GAGGGGAGCCTCATCCTCAACCTTCGACAAGGCTACAATCTGGAGCCAGAGCTTCAACGCAACCTGGGGC TGGTGGAGACCATCACCGGCTTCCTCAAAATCAAGCACTCTTTTGCACTCGTGACCCTGGGATTTTTCAA GAACCTCAAACTAATCCGGGGAGATTCCATGGTGGATGGGAACTACACTCTGTACGTGTTAGACAACCAG AACCTGCAACAGTTGGGGTCTTGGGTGGCCGCAGGGCTCACCATTCCCGTGGGCAAAATATACTTCGCCT TCAACCCACGCCTCTGCCTGGAGCACATATACCAACTGGAAGAGGTGACCGGAACCAGAGGCCGGCAAAG CAAGGCTGAAATCAACCCCCGGACCAATGGAGATCGTGCTGCCTGCCAGACTCGCACTCTGCGCTTTGTG TTCAACCTGACCGAGGAGGACCGCATCCTCCTGCGCTGGGAGCGCTATGAGCCACTGGAGGCCCGTGACC TGCTCAGCTTCATCGTCTATTACAAGGAGTCCCCATTCCAGAATGCCACAGAACACGTGGGTCCAGACGC CTGTGGAACTCAGAGCTGGAATCTGTTGGATGTGGAACTACCCCTCAGCCGCACCCAGGAGCCCGGGGTG ACCCTAGCCCCCCTAAAGCCCTGGACGCAGTATGCTGTGTTTGTGCGAGCCATCACACTGACCACGGCTG AGGACAGCCCCCATCAGGGAGCCCAGAGCCCCATTGTCTACCTGCGAACATTACCTGCAGCACCCACAGT ACCCCAGGACGTCATCTCCACCTCCAATTCCTCTTCACACCTCCTGGTGCGCTGGAAGCCGCCGGTCCAG CGCAATGGAAACATCACCTATTACCTGGTGTTGTGGCAGCGGCTAGCAGAGGACGGTGACCTCTACATCA ATGACTACTGCCACCGCGGTCTGCGGCTGCCCACCAGCAGCCACGACACACGCTTCGACCGTGAAGACCC GGCGCTGGAGGCTGAGCCGGAGCAAGGCTGCTGTCCTTGCCAACACTCGCCTCCTGGTCAGGCCCTGCCT GCACTGGAGGCACAAGAGGTCACCTTCCAGAAAAAGTTTGAAAACTTCCTACACCATGCCATCACCATCC CCAAGGCCCCGTGGAAAGTGACATCCATCAACAAGAACCCCCAAAGGGATTCGGAGAGGCACCGCCGGGA AACTGGGCTTCTCAGACTAGGGAAAAACAACTCGGATTTTGAGATTCATGAGGACAAGGTTCCCCGGGAG CGAGCAGTATTGAGCGGCTTGCGACACTTCACAGAATACAGGATTGACATCCATGCCTGCAACCACGCGG CACACACTGTGGGCTGCAGCGCTGCCACCTTCGTCTTTGCACGCACCATGCCACATAGAGAGGCCGATGG CATCCCAGGGAAAGTGGTCTGGAAGGCGGTTGGCAAGAGCAGTGTCATCTTGCATTGGCTTGAGCCACCT GACCCCAATGGGCTCATCCTCAAGTATGAAATCAAGTACCGCCGCCTTGGAGAGGAGGCCACAGTGCTTT GTGTGTCCCGTCTTCGATATGCCAAAGTTGGTGGGGTCCACCTGGCTCTGCTGCCTCCTGGAAACTACTC TGCTAAAGTTCGGGCCACCTCATTGGCTGGCAATGGCTCCTGGACTGACGGTGTTGCCTTCTACATCACC GGTCCAGAGGAAGAGGATACTGGGGGACTGCGCATCCTCCTCACTGTCACCCCTGTCGGCTTCATGCTGC TCGTGATGCTTGCTGCCCTTGGTTTCTTCTACAGCAAAAAGAGAAACAGCACGCTGTACACATCCGTGAA CCCGGAGTATTTTAGTGCATCCCACATGTATGTCCCCGACGAGTGGGAGGTCCCTCGGGAGCAGATAGCC ATCATCCGCGAGCTGGGCCAGGGCTCTTTTGGGATGGTATATGAGGGACTGGCACGAGGACTTGAGGCTG GAGAGGAGTCTACACCTGTAGCCCTGAAGACAGTTAATGAGCTGGCCAGCGCTCGGGAACGCGTTGAATT CCTCAAGGAAGCATCTGTCATGAAGGCCTTCAAGTGTCACCATGTGGTCCGTCTCCTGGGTGTGGTGTCT CAGGGCCAGCCAACTTTGGTCATCATGGAGTTAATGACCCGTGGAGACCTCAAGAGCCATCTCCGATCTC TGAGACCTGAGGCAGAGAACAACCCTGGCCTCCCACAGCCAGCACTCAGTGACATGATTCAGATGGCTGG TGAGATTGCAGATGGCATGGCCTATCTTGCTGCCAAGAAGTTTGTGCACCGGGACCTGGCAGCCCGCAAC TGCATGGTGTCTCAGGATTTTACCGTCAAAATTGGTGACTTCGGAATGACTCGGGATGTGTACGAGACAG ACTATTACCGCAAGGGTGGAAAGGGGCTACTGCCTGTGCGCTGGATGGCCCCTGAGTCCCTCAAAGACGG AATCTTCACCACTCACTCAGACGTCTGGTCCTTTGGTGTGGTGCTCTGGGAGATTGTGACCCTAGCTGAA CAACCGTACCAGGGCCTATCAAACGAGCAGGTGCTGAAGTTTGTCATGGACGGTGGAGTTCTAGAGGAGC AAGAGGATTGTCCAATTCAGCTACAGGAGCTGATGAGACGCTGCTGGCAGCATACTCCACGCCTGCGCCC GACTTTCGTCCACATCCTGGATCGCATACAGGATGAACTTCGTCCCTCTTTCCGGCTCTGCTCCTTCTAC TACAGCCCCGAGTGTCAGCGGGGCCAGGCCTCCTTGCTGCCTACTGAGGCAGAGCCTGACTCCCCACCAA CCTTAAATGGAGCTTCAGACTATGGTCCCCCAAATGGGGACCCAGGACACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_022212
Insert Size	3903 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022212.2, NP_071548.2
RefSeq Size	4997 bp
RefSeq ORF	3903 bp
Locus ID	60663
UniProt ID	Q64716
Gene Summary	member of the insulin receptor family [RGD, Feb 2006]

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