Gfap (NM_017009) Rat Untagged Clone

CAT#: RN206398

Gfap (untagged ORF) - Rat glial fibrillary acidic protein (Gfap), (10 ug)



  "NM_017009" in other vectors (3)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
chicken Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody
    • 100 ul

CNY 5,049.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN206398 representing NM_017009
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGAGACGTATCACCTCTGCACGCCGCTCCTATGCCTCCTCCGAGACGATGGTCAGGGGCCATG
GTCCTACCAGACACCTGGGTACCATTCCGCGCCTCTCCCTGTCTCGAATGACGCCTCCACTCCCTGCCAG
GGTAGACTTCTCCCTGGCCGGGGCGCTCAATGCCGGCTTCAAAGAGACTCGGGCCAGCGAGCGTGCGGAG
ATGATGGAGCTCAATGACCGCTTTGCTAGCTACATCGAGAAGGTCCGCTTCCTGGAACAGCAAAACAAGG
CGCTGGCAGCTGAGCTGAACCAGCTTCGAGCCAAGGAGCCCACCAAACTGGCTGACGTTTACCAGGCAGA
ACTTCGGGAGCTGCGGCTGCGTCTGGACCAGCTTACTACCAACAGTGCCCGGCTGGAGGTGGAGAGGGAC
AATCTCACACAGGACCTCGGCACCCTGAGGCAGAAGCTCCAAGATGAAACCAACCTGAGGCTGGAGGCGG
AGAACAACCTGGCTGTGTACAGACAGGAGGCGGATGAAGCCACCTTGGCTCGTGTGGATCTGGAGAGGAA
GGTTGAGTCGCTGGAGGAGGAGATCCAGTTCTTGAGGAAGATCCATGAGGAGGAAGTTCGAGAACTCCAG
GAGCAGCTGGCCCAGCAGCAGGTCCACGTGGAGATGGATGTGGCCAAGCCAGACCTCACAGCGGCTCTGA
GAGAGATTCGCACTCAGTACGAGGCAGTGGCCACCAGTAACATGCAAGAAACAGAAGAGTGGTATCGGTC
CAAGTTTGCAGACCTCACAGACGTTGCTTCCCGCAACGCAGAGCTGCTCCGCCAGGCCAAGCACGAGGCT
AATGACTATCGCCGCCAACTGCAGGCCTTGACCTGCGACCTTGAGTCCTTGCGCGGCACGAACGAGTCCT
TGGAGAGGCAAATGCGCGAACAGGAGGAGCGCCACGCGCGGGAGTCGGCGAGTTACCAGGAGGCACTCGC
TCGGCTGGAGGAGGAGGGCCAAAGCCTCAAGGAGGAGATGGCCCGCCACCTGCAGGAGTACCAGGATCTA
CTCAACGTTAAGCTAGCCCTGGACATCGAGATCGCCACCTACAGGAAATTGCTGGAGGGCGAAGAAAACC
GCATCACCATTCCTGTACAGACTTTCTCCAACCTCCAGATCCGAGAAACCAGCCTGGACACCAAATCTGT
GTCAGAAGGCCACCTCAAGAGGAACATCGTGGTAAAGACGGTGGAGATGCGGGATGGCGAGGTCATTAAG
GAGTCGAAGCAGGAGCACAAGGATGTGATGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_017009
Insert Size 1293 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017009.2, NP_058705.2
RefSeq Size 2714 bp
RefSeq ORF 1293 bp
Locus ID 24387
UniProt ID P47819
Gene Summary intermediate filament protein specific for astrocytes [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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