Ambra1 (NM_001134341) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Nyw1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206199 representing NM_001134341 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTTGTCCCAGAGAAGAATGCTGTACGAATCCTCTGGGGCCGAGAGCGGGGCACTCGTGCCATGG GTGCCCAGCGGCTTCTGCAGGAGCTGGTGGAGGATAAGACTCGATGGATGAAATGGGAGGGCAAGAGAGT AGAACTTCCTGATAGTCCACGCTCGACCTTCTTACTGGCCTTCAGCCCAGACAGGACTCTCTTGGCGTCC ACACATGTGAACCATAACATCTATATTACAGAGGTTAAGACTGGCAAGTGTGTTCACTCTCTGATTGGAC ACCGCCGTACTCCATGGTGTGTCACTTTTCATCCCACTATCTCAGGCCTTATTGCTTCTGGCTGCCTAGA TGGGGAAGTTAGGATTTGGGACTTACATGGTGGCAGTGAAAGCTGGTTCACAGATAGCAACAATGCCATA GCTTCTTTGGCTTTCCACCCTACGGCTCAGCTCCTGCTGATCGCCACTGCAAATGAGATCCATTTCTGGG ATTGGAGTCGTCGGGAACCCTTTGCTGTGGTGAAAACAGCTAGTGAGATGGAGCGTGTTCGTCTGGTGAG ATTTGATCCACTTGGACACTACTTACTTACAGCTATTGTTAACCCCTCTAATCAGCAGGGTGATGATGAA CCAGAAATCCCTATAGATGGAACAGAATTATCTCATTACCGTCAGCGTGCCCTCCTGCAATCACAGCCAG TTCGCCGGACACCCCTCCTCCACAATTTCCTGCATATGCTGTCCTCCCGCTCCTCTGGCATCCAGGTGGG AGAGCAAAGCACAGTACAAGATTCTGCTACCCCCTCACCCCCACCGCCTCCCCCTCAGCCCTCCACGGAG CGTCCCAGGACTTCCGCTTACATCAGACTCCGACAGCGGGTCAGTTACCCCACCACAGTTGAGTGCTGCC AGCACCCTGGGATCCTGTGCCTTTGCAGCCGTTGTGCTGGCACTCGAGTTCCTTCCCTCTTGCCACACCA GGACAGTGTCCCCCCTGCTTCTGCCAGAGCTACTACCCCTTCCTTTTCTTTTGTACAGACCGAGCCCTTC CATCCCCCGGAGCAGGCTTCATCAACGCAGCAGGACCAAGGCCTCCTGAACCGGCCGTCTGCCTTCAGTA CAGTCCAGAGCAGCACTGCGGGCAACACGCTCCGCAACCTCAGTCTGGGTCCTACCCGTCGCTCTTTGGG GGGCCCACTGTCCAGCCACCCATCTAGGTATCACAGAGAACTAGCCCCTGGGCTGACAGGTTCTGAGTGG ACCCGGACAGTACTCACTCTGAACTCACGTTCTGAGGTAGAATCTATGCCCCCACCGAGGACCAGTGCCT CCTCAGTGAGTTTGCTTTCTGTACTGAGACAGCAGGAAGGTGGCTCTCAGGCATCTGTGTATACTTCAGC CACAGAAGGGAGGGGTTTTCCATCATCAGGGTTGGCAACTGAGTCAGATGGAGGGAATGGTTCCAGCCAA AACAACTCAGGCAACATTCGCCATGAGCTTCAGTGTGACCTGAGACGCTTTTTTTTGGAGTATGACAGGC TGCAGGAGCTGGATCAGAGCCTGAGTGGGGAAACTCCCCAGACCCAACAGGCCCAGGAAATGCTCAACAA TAATATTGAATCTGAGAGACCAGGTCCTTCCCATCAGCCTACCCCACACAGCAGTGAGAACAACTCCAAC CTTTCTCGGGGCCACCTTAATCGATGCCGTGCTTGCCATAATCTTCTAACCTTCAACAATGATACCCTTC GTTGGGAAAGAACCACACCTAACTACTCATCCGGTGAGGCTAGCTCTTCCTGGCATGTCTCCACTACTTT TGAGGGCATGCCACCGAGTGGCAACCAACTACCACCTCTTGAGCGGACTGAGGGCCAAATGCCTAACTCC AGCAGGCTGGAGTTGAGCAGTTCTGCTAGTTCACAAGAGGAGAGGACTGTGGGGGTGGCCTTTAACCAGG AGACCGGCCATTGGGAACGAATTTATACCCAGTCCAGCAGATCTGGAACTGTATCACAGGAGGCCTTACA TCAAGATATGCCTGAGGAAAGCTCTGAGGAAGACTCCCTGAGGAGGAGGCTGCTGGAGTCTTCCCTCATT TCATTATCCCGTTATGATGGAGCAGGATCCAGAGAGCACCCAATTTACCCAGACCCAGCGAGATTATCTC CTGCTGCATACTACGCCCAGAGGATGATCCAGTATCTCTCAAGGAGAGACAGTATTCGCCAGCGCTCCAT GCGCTACCAACAGAATCGGCTTCGTTCTTCCACCTCCTCTTCTTCCTCAGACAACCAGGGTCCATCAGTA GAGGGAACCGACTTGGAATTTGAGGACTTTGAGGACAATGGTGACAGATCTAGGCACCGAGCTCCCCGAA ATGCCCGAATGTCTGCACCATCACTTGGACGCTTTGTCCCAAGGCGTTTTTTGCTGCCTGAGTACTTGCC TTATGCTGGGATTTTTCATGAGCGTGGACAGCCTGGCTTGGCTACTCATTCTTCTGTTAACAGGGTCCTG GCAGGGGCAGTAATTGGAGATGGACAGTCTGCTGTGGCCAGTAACATTGCCAATACTACCTACAGGCTCC AGTGGTGGGACTTCACCAAGTTTGACCTTCCAGAAATCAGTAATGCCTCCGTGAATGTCCTGGTACAGAA CTGCAAGATCTACAATGATGCCAGCTGTGACATTTCTGCAGATGGCCAGCTCCTGGCAGCTTTCATCCCG AGTAGCCAGAGGGGCTTTCCTGATGAAGGCATCCTGGCAGTGTATTCCCTGGCCCCCCATAACTTGGGGG AAATGCTCTACACCAAGCGATTTGGTCCCAATGCCATTTCTGTAAGCCTGTCCCCAATGGGCCGATACGT TATGGTGGGCTTGGCCTCACGAAGGATCCTGCTGCACCCCTCTACAGAGCACATGGTAGCCCAGGTCTTC AGGCTGCAGCAGGCCCATGGTGGTGAGACTTCCATGAGGAGAGTTTTCAACGTCCTTTACCCCATGCCTG CGGACCAGCGGAGACACGTCAGCATCAACTCTGCCCGCTGGCTACCTGAGCCAGGGCTCGGTTTGGCCTA TGGCACCAACAAAGGAGACCTGGTGATCTGCCGACCAGAGGCCTTAAACTCTGGCATTGAGTACTACTGG GACCAGCTAAATGAAACGGTCTTCACTGTCCACTCAAGTAGCAGGAGCAGCGAGCGGCCTGGAACCAGCA GAGCCACATGGCGGACAGACAGAGACATGGGCTTGATGAATGCAATTGGGCTGCAACCTCGGAACCCTAC CACCTCTGTGACATCTCAGGGCACCCAGACTCTGGCCCTCCAGTTGCAGAATGCTGAAACACAGACTGAG AGGGAGGAAGAGGAACCAGGGACAGCATCCTCAGGTCCTGGTGAAGGCGAGGGCTCGGAATATGGTGGCA GTGGGGAAGATGCCCTCAGCCGGATCCAGAGGCTAATGGCCGAGGGTGGCATGACAGCTGTGGTACAGCG AGAACAGAGTACCACCATGGCCTCCATGGGTGGCTTCGGCAACAACATCATCGTTAGCCACCGCATTCAC CGCAGCTCCCAGACAGGCACAGAGTCAGGGGCTGCTCGTACCTCCTCACCCCAGCCCTCCACCTCTCGAG GACTACTCTCAGAACCTGGGCAACTGGCAGAGCGAGGACTAAGCCCCCGGACAGCCTCCTGGGACCAGCC TAGTACCTCTGGAAGGGAACTACCCCAGCCAGCCCTGTCCTCTTCCTCCCCTGTCCCCATTCCAGTCCCC CTTGCCAGCAATGAGGGACCAACCATGCACTGCAATGTGACCAATAACAGTCACCTTCCTGAGGGTGATA GTAGCAATGTGGGGGAAGCTGCAGGCCCTAGTGGGGAGCCACGGAACCGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134341
Insert Size	3903 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134341.1, NP_001127813.1
RefSeq Size	5290 bp
RefSeq ORF	3903 bp
Locus ID	59319

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