United States
Germany
Japan
United Kingdom
China
Yod1 (NM_001008889) Rat Untagged Clone
CAT＃: RN206073
Yod1 (untagged ORF) - Rat YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) (Yod1), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | hshin7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN206073 representing NM_001008889
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGGCTCCGCTGCAAGGCCAAGGGTGGCACCCACGTTTTGCAGGGTCTTTCCAACCGGACCCGCT TACGGGAACTGCAGGGCCAAATCGCCGCTATCACCGGCATCGCTCCTGGCAGTCAGCGAATCCTCGTCGG CTACCCACCAGAGTGCCTGGATCTCAGCGACCGGGACATCACTCTCGGGGACCTGCCCATCCAGTCAGGT GACATGCTGATTGTTGAAGAAGACCAAACCAGACCAAAAGCTTCACCTGCGTTTTCAAAACATGGTGCTC CTAGTTATGTCAGGGAAACTCTGCCTGTGCTTACCAGAACCGCAGTCCCAGCAGACAACTCTTGCCTCTT TACCAGTGTGTACTATGTAGTTGAAGGAGGAGTCTTGAATCCAGCTTGTGCCCCTGAGATGAGACGCCTC ATAGCACAAATTGTAGCCAGTGATCCAGACTTGTATAGTGAGGCAATACTGGGAAAGACAAACGAAGAGT ACTGTGATTGGATCAGAAGGGATGATACTTGGGGGGGAGCAATTGAGATATCAATCCTGTCCAAGTTTTA TCAATGTGAAATATGTGTAGTAGATACACAGACAGTCAGAATTGATCGTTTTGGGGAAGATGCAGGCTAT ACCAAAAGGGTTCTACTCATCTACGATGGCATTCACTACGATCCGCTTCAGCGAAACTTCCCTGATCCGG ATACCCCTCCTCTGACCATTTTCTCCTCAAATGATGATATTGTTCTCGTACAAGCACTGGAATTAGCTGA TGAAGCTAGAAGAAAGAGACAGTTCACTGATGTAAACCGCTTCACCCTGAGATGCATGCTGTGTCAGAAG GGCCTAACCGGACAAGCTGAAGCAAGGGACCATGCCAGGGAGACAGGCCATACCAACTTTGGAGAGGTGT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001008889 |
| Insert Size | 912 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001008889.1, NP_001008889.1 |
| RefSeq Size | 912 bp |
| RefSeq ORF | 912 bp |
| Locus ID | 363982 |
| UniProt ID | Q32Q05 |
| Gene Summary | Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR206073 | Yod1 (Myc-DDK-tagged ORF) - Rat YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) (Yod1), (10 ug) |
CNY 3,600.00 |
|
| RR206073L3 | Lenti ORF clone of Yod1 (Myc-DDK-tagged ORF) - Rat YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) (Yod1), (10 ug) |
CNY 6,080.00 |
|
| RR206073L4 | Lenti ORF clone of Yod1 (mGFP-tagged ORF) - Rat YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) (Yod1), (10 ug) |
CNY 6,650.00 |
