Cdc42bpb (NM_053620) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206006 representing NM_053620 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCAAGGTGCGGCTCAAGAAGCTGGAGCAGCTGCTCCTGGACGGGCCGTGGCGCAATGAGAGCT CCCTGAGCGTGGAGACGCTGCTGGACGTGCTGGTGTGCCTTTACACCGAGTGCAGCCACTCGGCGCTGCG CCGCGACAAGTATGTGGCGGAGTTCCTCGAGTGGGCTAAGCCATTCACCCAGCTCGTGAAGGACATGCAG CTTCATCGGGAGGACTTTGAGATCATCAAAGTGATTGGAAGAGGAGCTTTTGGTGAGGTTGCTGTTGTCA AAATGAAGAACACTGAACGAATTTATGCAATGAAAATTCTCAACAAATGGGAGATGCTAAAGAGAGCAGA GACAGCTTGCTTTCGAGAAGAGAGGGATGTGCTGGTGAACGGTGACTGCCAGTGGATCACTGCGCTACAC TACGCCTTTCAAGATGAGAACTACCTGTACTTGGTTATGGATTACTATGTGGGTGGTGACTTGCTGACCC TGCTGAGTAAGTTTGAAGACAAACTTCCAGAAGACATGGCGAGGTTCTACATTGGCGAGATGGTGTTGGC CATCGACTCGATCCACCAGCTCCACTATGTGCACAGAGACATTAAACCCGACAACGTCCTTCTGGATGTC AACGGTCACATCCGCCTGGCTGACTTTGGCTCGTGCTTGAAGATGAACGATGACGGCACTGTTCAGTCTT CCGTGGCTGTGGGCACACCTGACTACATCTCACCAGAGATCCTGCAGGCCATGGAGGATGGCATGGGCAA ATACGGGCCCGAGTGTGACTGGTGGTCCCTGGGCGTCTGCATGTATGAGATGCTGTATGGAGAAACGCCA TTTTATGCAGAGTCTCTGGTGGAGACATATGGAAAGATCATGAACCACGAGGAGCGGTTTCAGTTCCCAT CCCACGTCACTGACGTCTCTGAAGAAGCGAAAGACCTCATCCAGAGACTAATATGCAGCAGAGAGCGCCG GCTCGGGCAGAACGGGATAGAGGACTTTAAGAAACATGCATTCTTTGAAGGTCTAAATTGGGAAAATATA CGGAACCTGGAAGCACCCTACATTCCCGATGTGAGCAGCCCTTCTGACACGTCCAACTTCGATGTGGACG ATGACGTGCTGAGAAACATCGAAATCTTACCTCCCGGCTCTCACACGGGCTTCTCGGGACTGCACCTACC CTTCATTGGCTTTACATTTACGACAGAAAGCTGCTTTTCTGACCGAGGCTCTCTGAAGAGCATGATACAG TCTAACACGCTAACCAAAGATGAGGATGTGCAGCGGGATTTGGAGAACAGCTTGCAGATCGAAGCTTACG AGCGAAGGATACGAAGGCTGGAACAGGAGAAGCTAGAGCTGAGCCGGAAGCTGCAAGAGTCCACCCAGAC TGTGCAGTCCCTTCACGGTTCCACACGGGCCCTGGGCAACTCAAACCGTGACAAGGAAATCAAGAGGCTG AATGAAGAGCTTGAACGCATGAAGAGTAAAATGGCAGATTCAAACAGGCTCGAACGACAGCTCGAGGATA CCGTGACCCTTCGCCAGGAGCATGAAGACTCCACACAACGGCTGAAGGGCCTGGAGAAGCAGTACCGCCT GGCACGGCAGGAGAAGGAAGAATTGCACAAGCAATTGGTTGAAGCTTCAGAGCGATTGAAATCCCAGACC AAAGAACTCAAAGATGCTCATCAGCAGCGAAAACGGGCCCTGCAGGAGTTTTCAGAGCTCAATGAGCGCA TGGCAGAGCTGAGGTCACAGAAGCAGAAGGTGTCCCGGCAGCTCCGTGACAAGGAGGAGGAGATGGAGGT GGCCATGCAGAAGATTGACTCCATGAGGCAGGATATCCGCAAGTCTGAGAAGTCTAGGAAAGAGCTGGAA GCTCGGCTTGAGGACGCAGTCGCTGAGGCCTCCAAGGAGAGGAAGCTTCGAGAGCACAGTGAGAGCTTCT CTAAGCAGATGGAGCGCGAGCTCGAGACCCTCAAGGTGAAGCAAGGAGGCCGGGGGCCGGGTGCCACGTT AGAACATCAGCAGGAGATCTCCAAAATCAGGTCAGAGCTTGAGAAGAAAGTCTTGTTCTATGAGGAGGAG TTGGTCCGCCGAGAGGCCTCCCATGTGCTAGAAGTAAAGAATGTGAAGAAGGAGGTGCACGAATCTGAAA GCCACCAGTTGGCCCTGCAGAAAGAGGTCCTGATGCTGAAAGATAAGTTAGAAAAGTCAAAACGAGAACG GCACAGTGAGATGGAGGAGGCCATAGGTGCCATGAAAGACAAGTACGAACGGGAGAGGGCCATGCTGTTC GACGAGAACAAGAAGTTAACAGCAGAAAACGAAAAGCTCTGTTCCTTTGTGGATAAACTAACAGCCCAAA ACAGACAGCTGGAGGACGAGCTGCAGGACCTGGCCTCCAAGAAGGAGTCGGTCGCTCACTGGGAGGCGCA GATTGCAGAGATTATTCAGTGGGTCAGCGATGAGAAAGATGCCAGAGGCTACCTTCAAGCACTTGCTTCT AAGATGACCGAAGAGCTTGAGACTTTGAGAAGCTCTAGTTTGGGATCCAGGACACTGGACCCACTCTGGA AGGTCCGTCGGAGCCAGAAGCTGGACATGTCTGCGCGGCTGGAGTTGCAGTCCGCTCTCGAGGCAGAGAT CCGTGCCAAGCAGCTGGTTCACGAGGAGCTGAGGAAGGTCAAAGACACCAGCCTAGCCTTCGAAAGCAAA CTGAAGGAGTCAGAAGCCAAAAACAGGGAATTATTAGAAGAAATGCAAAGTCTGAAGAAAAGGATGGAAG AAAAATTTAGAGCAGATACAGGGCTCAAACTTCCAGATTTCCAGGATCCTATCTTTGAGTACTTCAACAC TGCTCCTCTTGCACATGACCTGACTTTTAGAACTAGCTCAGCTAGTGATCAGGAAACACAGGCTTCAAAG TTGGACTTGTCCCCATCAGTGTCTGTGGCCACAAGCACAGAACAGCAGGAGGATGCGGCTCGTTCACAGC AGAGGCCGTCTACTGTGCCGCTGCCCAACACACAGGCCCTCGCCATGGCTGGACCAAAGCCCAAGGCACA CCAGTTCAGCATCAAGTCCTTCCCCAGCCCCACCCAGTGTAGCCACTGTACGTCCTTGATGGTTGGGCTG ATCCGACAGGGCTATGCCTGTGAGGTCTGCGCGTTTTCCTGCCATGTGTCCTGCAAAGACAGCGCTCCCC AGGTGTGCCCCATACCTCCTGAGCAGTCCAAGCGGCCTCTCGGCGTAGACGTGCAGAGGGGCATAGGCAC AGCCTACAAGGGCTACGTCAAGGTCCCGAAGCCCACAGGCGTGAAGAAAGGGTGGCAGCGGGCTTACGCA GTGGTCTGTGACTGCAAGCTCTTCCTGTACGACCTGCCAGAAGGGAAGTCCACCCAGCCTGGGGTCATCG CCAGTCAAGTCTTAGACCTCAGAGATGACGAGTTTGCTGTGAGCTCAGTCCTGGCCTCAGATGTCATCCA TGCTACACGCCGAGACATCCCGTGTATATTCAGGGTGACGGCCTCTCTCTTAGGTTCACCTTCTAAGACC AGCTCACTGCTCATCCTGACAGAGAACGAGAATGAAAAGAGGAAGTGGGTTGGGATCCTGGAAGGGCTGC AGGCTATCCTCCACAAGAACCGGCTGAGGAGCCAGGTGGTGCACGTGGCACAGGAAGCGTACGACAGCTC ACTGCCGCTCATCAAGACCGTCCTGGCTGCCGCTATTGTGGATGGAGACAGAATTGCGGTCGGCCTGGAA GAAGGGCTCTACGTCATTGAGCTCACCCGAGATGTGATCGTCCGCGCTGCTGACTGCAAGAAGGTATACC AGATCGAGCTGGCGCCCAAGGAGAAGCTCATCCTCCTCCTCTGTGGCCGGAACCACCACGTGCACCTCTA CCCCTGGACCTCCTTCGATGGAGCAGAAGCTAGCAACTTTGACATCAAGCTCCCAGAAACGAAGGGCTGC CAGCTCATAGCAACAGGGACGCTGAGGAAGAGCTCGTCCACCTGCCTGTTTGTCGCCGTGAAGCGGCTGG TCCTTTGCTACGAGATCCAGAGAACTAAGCCTTTCCACCGGAAGTTCAATGAGATCGTGGCCCCGGGACA TGTGCAGTGGATGGCCATGTTCAAGGACAGGCTCTGTGTTGGCTACCCCTCCGGGTTCTCTCTGTTGAGC ATCCAGGGGGACGGGCAGCCTCTAGACCTGGTAAATCCCGCTGACCCCTCGCTCGCGTTCCTCTCACAGC AGTCTTTCGATGCCCTCTGTGCTGTAGAGCTCAAAAGTGAGGAGTACCTGCTCTGCTTCAGCCACATGGG ACTGTACGTGGACCCTCAAGGTCGGAGGTCACGCACGCAGGAGCTCATGTGGCCTGCGGCTCCTGTCGCC TGTAGTTGCAGCTCCTCCCACGTCACGGTGTACAGCGAATACGGGGTGGATGTCTTCGACGTGCGCACCA TGGAGTGGGTTCAGACCATCGGCCTGCGGAGGATAAGACCTCTGAACTCTGATGGTAGCCTCAACCTGCT GGGCTGTGAGCCCCCACGCCTCATCTACTTCAAAAACAAGTTCTCAGGCACCGTCCTCAATGTGCCCGAT ACCTCGGACAACAGCAAGAAGCAGATGCTGCGGACGCGGAGCAAACGGCGCTTCGTCTTCAAGGTTCCAG AGGAAGAGAGGCTGCAGCAGCGGCGAGAGATGCTCAGAGACCCGGAACTGAGATCCAAAATGATATCCAA TCCGACCAACTTCAACCACGTGGCTCACATGGGCCCTGGCGATGGGATGCAGGTGCTCATGGACCTGCCT CTGAGTGCTGCGCCCACAGCCCAGGAGGAAAAGCAGGGTCCTGCCCCAACAGGCCTCCCTCGGCAGCTCC CATCCAGGAACAAGCCCTATGTCTCATGGCCGTCCTCAGGTGGGTCTGAGCCTGGAGTGCCTGTGCCTCT GAGGAGCATGTCTGACCCCGACCAGGATTTTGACAAAGAGCCTGACTCTGACTCCACGAAGCACTCGACT CCGTCCAACAGCTCCAACCCTAGCGGCCCCCCGAGCCCCAACTCACCGCACCGGAGCCAGCTCCCTCTGG AAGGCCTGGACCAGCCAGCCTGCGACGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053620
Insert Size	5142 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053620.3, NP_446072.2
RefSeq Size	6397 bp
RefSeq ORF	5142 bp
Locus ID	113960
UniProt ID	Q7TT49
Gene Summary	serine/threonine protein kinase; mediates myosin light chain phosphorylation [RGD, Feb 2006]

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