Rapgef6 (NM_001107003) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205672 representing NM_001107003 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCGCCCGTGGACCCTGGCGCTCGGCAGGCACTGAGGAAGAAGCCGCCGGAGCGGACTCCTGAGG ACTTAAATATTATTTATTCCTATCTCCATGGGATGGAAATACTATCAAACCTCAGGGAGCATCAGCTTAG GTTAATGTCTACAAGAGCACGCTATGAGAGATACAATGGCAATCAAATGCTTTTTTGTTCAGAAACTATT GCCAGATGTTGGTATATCCTGCTTTCTGGATCTGTACTTGTAAAAGATTCTATGGTCCTTCCTCCTTGCA GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTATTAGAACCTTCAGAAATGATTGT GGTAGAAAATTCCAAAGACAATGAAGATAGTATATTACAAAGAGAAATTCCTGCCAGACAGTCTCGACGA AGGTTTCGGAAAATTAACTATAAGGGAGAACGCCAAACCATAATCGATGGGGTGGATGTTAATAACTACC TTTCGCTTCCAGCAGATCTTACCAAGATGCACCTCACGGACAACCCTCATCCACAGGTCACTCATGTGAC TTCTAGTCAGTCTGGCTGTAGCATTGCCAGTGACTCTGGGAGCAGCAGTTTATCCGATATCTATCAGGCT ACAGAGAGTGAAGTAGGAGATGTAGATTTAACCCGTCTTCCTGAAGGACCTGTTGATTCTGAGGATGAAG AGGAGGAGGAGGAAGAGATTGATCGAACAGATCCACTGCAGGGTCGAGATCTTGTCCGGGAATGTCTTGA AAAGGAACCTGCAGACAAAACTGATGATGATGTTGAGCAGTTACTTGAGTTCATGCACCAGCTACCTGCC TTTGCGAACATGACCATGTCTGTAAGGAGAGAGCTCTGCTCTGTCATGATTTTTGAAGTAGTAGAGCAGG CTGGGGCTGTTATTCTTGAAGATGGGCAAGAGCTTGACTCGTGGTATGTCATTTTAAATGGCACTGTAGA AATTAGCCATCCAGATGGGAAAATTGAAAATCTCTTCATGGGAAATAGTTTTGGAATCGTGCCCACTCTG GATAAGCAGCACATGCGTGGAGTTGTCAGAACCAAAGTCGATGATTGTCAGTTTGTCTGCATAGCCCAAC AGGACTATTGGAGAATTTTAAATCATGTTGAAAAAAACACCCATAAAGTTGAGGAAGAGGGAGAAATCGT CATGGTACATGAGCACCGTGAGCTAGACCGGAGTGGAACTAGAAAAGGACACATCGTGATCAAGGCGACA CCTGAGCGTCTCATCATGCATTTGATAGAAGAGCATTCTATTGTGGACCCAACTTATATAGAAGATTTCC TATTAACTTACAGAACATTTCTTGAAACTCCTTTGGATGTTGGGATCAAGCTTTTAGAATGGTTTAAGAT TGACAGCTTAAGGGATAAGGTGACCCGGATTGTACTCTTATGGGTAAATAATCATTTTAATGATTTTGAA GGTGATCCTGCTATGACTCGATTTCTAGAAGAATTTGAAAAAAATTTGGAAGATACAAAAATGAACGGTC ACCTCAGATTACTGAACATCGCCTGTGCTGCAAAGGCTAAGTGGAGGCAGGTTGTACTGCAGAAGGCGTC CCGTGAGTCCCCGCTACACTTCAGCCTCACGGGAGGCAGCGAGAAGGGATTTGGTGTCTTTGTAGAAGTA GTGGAGCCTGGTACCAAAGCTGCTGACGCAGGACTGAAACGGGGTGATCAGATAATGGAAGTCAATGGGC AAAACTTTGAGAATATTACGTTTGCTAAAGCCCTTGAAATTTTGAGGAATAATACTCATCTTGCACTTAC TGTGAAGACCAACATTTTTGTGTTCAAAGAATTGCTCAGTAGGACTGAACAAGAGAAATCTGGTGTCCCT CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGTCATTCTATTCAAGATGTCCCAGGGGATATTGAGC AGGTGCCACAGGAGAAAGGAAACAAGAAAATTAAAGCAAATACCGTTTCAGGTGGAAGAAATAAAATCAG GAAAATTTTGGATAAAACAAGATTTAGTATCTTGCCTCCAAAACTATTTAGTGACGGAGGCCTGAGTCAA TCACAAGATGACAGCATTGTGGGAACCAGGCACTGTAGGCACAGCCTGGCTATCATGCCAATTCCTGGAA CACTGTCATCCAGCAGCCCAGATCTCTTACAGCCTACCACTAGCATGTTGGACTTTTCCAATCCTTCAGA TATTCCTGATCAAGTTATAAGAGTTTTCAAAGCAGATCAACAAAGCTGCTACATTATCATCAGTAAAGAT ACCACAGCTAAAGAAGTGGTCTGCCAAGCTGTACAGGAATTTGGTTTGACTGGTGCATCTGACACATACT CTCTCTGTGAAGTTTCTGTTACTCCTGAGGGTGTTATAAAGCAGAGAAGACTTCCGGACCAGTTCTCCAA ATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACCTAAAAAATAACATGGAGACAGAAACATTGTGT TCTGATGAAGATGCCCAGGAACTACTGAAAGAAAGCCAGCTCTCCATGCTGCAGCTCAGCACCATCGAGG TTGCCACCCAGCTGTCAATGAGGGACTTTGATTTGTTTCGTAACATTGAGCCTACTGAATATATTGATGA TCTTTTTAAATTAGATTCCAAAACAGGAAATACTCACTTGAAACAGTTTGAGGATATTGTAAACCAAGAG ACATTCTGGGTTGCCACAGAGATTCTAAGTGAGTCAAACCAGCTGAAAAGAATGAAGATTATTAAACATT TTATTAAAATTGCCCTTCATTGCCGAGAATGCAAGAATTTCAATTCCATGTTTGCAATAATAAGTGGCTT GAACCTGGCACCAGTAGCACGACTCAGAGGCACCTGGGAAAAGTTACCAAGCAAATATGAGAAACACCTT CAAGATCTACAAGACCTTTTTGATCCATCTAGAAACATGGCAAAATACAGAAATATTCTTAGTAGTCAGA GCATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATTCCTTCATGAAGGAAA TGACTCCAAAGTAGATGGCTTAGTAAACTTCGAGAAGCTAAGAATGATTGCCAAGGAAATCCGTCATATT ATTCGAATGACTTCTGCTAACATGGACCCTGCCATGATGTTTCGCCAGAGGTCATTGAGTCAAGGCAGCA CAAATTCTAACATGCTAGATGTTCAGGGAGGGGCTCACAAAAAAAGAGCACGCCGCAGCTCACTGCTGAA TGCCAAGAAACTGTATGAAGATGCCCAAATGGCAAGGAAAGTGAAACAGTATCTTTCTAGTCTGGACATA GACACAGAGGAGGAGAAGTTCCAGATGATGTCACTGCAGTGGGAGCCTGCATATGGTACCTTGACAAAGA GCTTAACTGAAAAAAAGTCAGCCAGATCATCTGAGATGTCTCCTGTGCCGTTGAGGTCAGCTGGCCACAC AGCTAAAGTCCACTTGCACCAGCCACACAGAGTGAGCCAGGTGCTTCAGGTGCCAGCAGTTAACTTGCAC CCCATCCGGAAGAAAGGACAAACCAGAGACCATGTGCTGAGTACAAATTTACCTCAGAAAGGTTTAGGAC CAACTGAAGAAGTGAGTGGTAAGAAACACACAGAAGATACTATTTCTGTAGCGTCCTCTCTACACTCTAG TCCTCCTGCATCTCCTCAGAGCTCCCCTCGCAAAGGTTGTACACTCACACCATCAAGTAAATGTGACAAC TTATCTGACTCCAGCCACAGTGAAATCTCCTCTCGGTCCAGCATTGTGAGCAATGGTTCTGTTGACTCCA TGTCGGCAGCTGGACAGGATGAACGCTGTTCGGCCCACAGCCTAGCAGTTCCTGAGCCCACCGGGGCATG GGAAAAGACAGATCACCCTTCGGGGATCTCAGACCACAGTCAGCTTGCTCACGGGTGGATGCTGTCAAAG CCATCTCTTATCAAGGGCGTAGCCGTCTCGTCTTCTCTGAGCAGCGAAGAGATGTCTCAGGAGCATGTTG TGCTGGAGGCAGCTGACAGTGGTCGGGGCAGTTGGACATCCTGTTCCAGCAGCTCCCATGACAACTTCCA GAGCCTTCAGAATCAAAAGAGCTGGGACTTTCTGAACTCCTACAGACACACGCATCTAGGCGACCCTATT GCTGAAGTTGAGCCCCCTGACTGTGAGCCCTGCGCCTGTCCTAAAGGTTGCTCAAGAACTTGTAGCCAGT GCAAAGGCAGCCTAGAGACGAACCAGCTGAGGCAGAGTTGGGCCTCATCCAGCTCACTGTCTGACACGTG GGAACCAAACTACGGGACAGTCAAGCGGAGGGTTTTGGAAAGTACACCAGCAGAGGCACCTGATGGCTTG GAGCCCAGGGATACCAGTGACCCCGTTTATAAAACTGTCACTTCCAGTACAGACAAGGGCTTGATTGTGT ACTGTGTCACCTCACCCAAGAAAGGTGATAGGCATAGGGAGCCACCTCCCACTCCTCCAGGATATCTGGG AATTTCCTTAGCGGACCTAAAGGAAGGATCCCACCCGCACCTAAAACCTCCAGACTATAGTGTGGCAGTA CAAAGATCAAAGATGATGCTTCTTAACAGCCTGTCTAGACTGCCGCCAGCTCCTCCCAGTAGCCACACCT CGGCCTGGGTTCCCTCGAAGATCGGATCCCAGCCTCAAAGGCATAGCTTGCAGCCGTCCCATCCCAAACT AGCAGATGTGGCTGATGCAGATAGCGAAGCAGATGAGAATGAGCAGGTGTCAGCAGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107003
Insert Size	4821 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107003.1, NP_001100473.1
RefSeq Size	5996 bp
RefSeq ORF	4821 bp
Locus ID	303141

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.