Sptan1 (NM_171983) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	A2a; IPF; Spna2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205584 representing NM_171983 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAAGTGGGGTCAAAGTGCTGGAAACAGCCGAGGACATCCAGGAGAGACGACAGCAGGTTCTGG ATCGGTACCACCGCTTCAAGGAGCTCTCTACCTTGCGGCGGCAGAAACTGGAGGATTCCTATCGCTTCCA GTTTTTTCAGAGAGATGCTGAGGAGCTGGAGAAGTGGATTCAGGAGAAGCTTCAAGTTGCCTCTGATGAG AACTACAAAGACCCCACCAACTTGCAGGGAAAGCTCCAGAAACACCAAGCCTTTGAAGCTGAAGTACAGG CCAACTCAGGAGCCATTGTGAAGCTGGATGAGACAGGAAACTTGATGATTTCTGAAGGGCACTTTGCATC TGAGACCATCCGGACACGTTTAATGGAGCTGCACCGCCAGTGGGAGTTGCTTTTGGAGAAGATGCGGGAG AAAGGAATCAAACTGCTGCAGGCACAGAAGCTGGTGCAGTATTTGCGGGAGTGTGAGGATGTAATGGACT GGATCAATGACAAGGAAGCAATTGTGACATCTGAGGAGCTGGGCCAGGATTTGGAGCATGTAGAGGTACT ACAGAAGAAGTTTGAAGAGTTTCAAACTGATCTGGCTGCTCATGAAGAAAGAGTTAATGAAGTAAACCAG TTTGCTGCCAAACTTATCCAGGAGCAGCACCCGGAAGAGGAGCTGATCAAGACCAAGCAGGAGGAGGTGA ATGCAGCTTGGCAGCGACTGAAAGGCCTGGCTCTTCAAAGGCAGGGGAAGCTCTTTGGTGCTGCCGAGGT TCAGCGCTTTAACAGGGATGTAGATGAGACCATTGGTTGGATTAAGGAGAAAGAGCAGTTAATGGCCTCT GATGACTTTGGCAGAGACTTAGCAAGTGTTCAAGCTCTGCTTCGGAAGCATGAGGGTCTGGAGAGAGATC TTGCTGCTCTAGAGGACAAGGTGAAAGCCCTGTGTGCCGAGGCTGACCGCCTGCAACAGTCACACCCTCT GAGTGCCAACCAGATCCAGGTGAAGCGAGAGGAACTAATTACCAACTGGGAGCAGATCCGAACTCTGGCC GCAGAGAGACATGCACGGCTTGATGACTCATACAGGCTTCAGCGCTTTCTTGCTGACTTCCGTGACCTCA CGAGCTGGGTGACTGAAATGAAAGCCCTCATCAATGCAGATGAACTGGCCAATGACGTGGCTGGTGCTGA GGCCCTGCTGGACAGGCATCAAGAGCACAAGGGTGAAATCGATGCTCATGAAGATAGCTTTAAGTCTGCA GATGAGTCTGGGCAGGCCCTACTCGCTGCTGGTCACTATGCCTCAGATGAAGTGAGGGAGAAGCTGAGCA TCCTCTCTGAGGAGAGAGCTGCCCTGCTGGAGCTGTGGGAGCTTCGGAGGCAGCAGTATGAGCAGTGCAT GGACTTGCAGCTCTTCTACCGAGACACTGAGCAGGTGGACAACTGGATGAGCAAACAGGAGGCATTCCTG CTAAATGAAGATTTGGGTGACTCCTTAGACAGTGTGGAAGCTCTTTTGAAGAAGCATGAGGACTTTGAGA AATCTCTCAGTGCCCAGGAAGAAAAAATCACAGCACTTGATGAGTTTGCAACCAAGCTTATTCAGAACAA CCACTACGCAATGGAAGATGTAGCCACTCGACGAGATGCTCTCCTGAGCCGCCGCAATGCCCTCCATGAG CGAGCCATGCATCGCCGGGCACAGCTGGCCGATTCCTTCCACCTGCAGCAGTTCTTCCGCGATTCCGATG AGCTCAAAAGTTGGGTCAATGAGAAGATGAAAACGGCCACTGATGAAGCTTACAAAGATCCGTCCAACCT GCAAGGGAAAGTCCAAAAGCACCAGGCTTTTGAGGCTGAGCTCTCAGCCAACCAGAGCCGTATTGATGCC CTAGAGAAAGCTGGGCAAAAACTAATAGATGTGAACCACTATGCCAAGGAAGAAGTAGCAGCTCGGATGA ATGAGGTCATCAGTTTGTGGAAGAAACTTCTAGAGGCCACAGAACTGAAAGGAGTCAAGCTCCGAGAAGC CAACCAGCAGCAACAATTTAATCGCAATGTTGAGGACATTGAGTTGTGGCTGTATGAAGTTGAAGGTCAC TTGGCTTCAGATGATTATGGTAAAGACCTCACTAATGTCCAGAACCTCCAGAAGAAGCATGCTCTGCTAG AGGCAGATGTTGCTGCTCACCAGGATCGAATTGACGGCATCACAATTCAGGCCCGCCAGTTCCAAGATGC TGGCCATTTCGATGCCGAAAACATTAAAAAGAAGCAAGAGGCCCTTGTAGCTCGCTATGAGGCTCTCAAG GAACCCATGGTGGCCCGGAAGCAGAAGCTGGCAGATTCTCTTCGTCTGCAGCAGCTCTTCCGAGATGTGG AGGATGAGGAAACCTGGATTCGAGAAAAGGAGCCTATTGCTGCGTCCACTAACAGAGGCAAAGATCTTAT TGGAGTCCAGAATCTGCTAAAGAAGCACCAAGCTTTACAGGCAGAAATTGCTGGCCATGAACCTCGCATC AAAGCAGTGACACAAAAGGGCAATGCCATGGTGGAGGAAGGCCATTTTGCTGCTGAGGATGTGAAGGCCA AACTGAGTGAGCTCAACCAGAAGTGGGAGGCACTGAAAGCCAAAGCCTCCCAGCGGAGGCAGGATCTGGA GGACTCACTACAGGCCCAGCAGTACTTTGCCGACGCCAATGAAGCTGAGTCCTGGATGCGGGAGAAGGAG CCCATTGTGGGCAGTACCGACTATGGGAAGGATGAAGACTCTGCTGAGGCTCTGCTCAAGAAGCATGAAG CTTTGATGTCCGATCTCAGTGCCTACGGCAGCAGCATTCAAGCTTTGCGAGAGCAGGCTCAGTCATGCCG GCAACAAGTGGCCCCCATGGATGATGAGACTGGCAAGGAGCTGGTCTTGGCTCTCTATGACTATCAAGAG AAGAGCCCTCGTGAGGTCACCATGAAGAAAGGGGATATCCTCACCTTGCTCAACAGCACAAACAAGGACT GGTGGAAAGTGGAAGTGAATGACCGTCAGGGTTTTGTGCCAGCTGCGTATGTGAAGAAGCTGGACCCCGC CCAGTCAGCCTCAAGGGAGAACCTCCTGGAAGAACAGGGCAGCATTGCTCTGCGGCAGGGGCAGATCGAC AACCAGACACGCATAACTAAGGAGGCCGGCAGTGTATCTCTGCGTATGAAACAGGTGGAAGAACTGTATC AGTCTCTGCTGGAGCTGGGTGAGAAGAGAAAAGGCATGTTGGAGAAGAGTTGCAAGAAGTTCATGTTGTT CCGGGAAGCGAACGAGCTACAGCAGTGGATCAACGAGAAGGAAGCTGCTCTAACGAGTGAAGAGGTTGGC GCTGACTTGGAGCAGGTCGAGGTGCTGCAGAAGAAGTTCGATGACTTCCAGAAGGATCTGAAAGCCAATG AGTCCCGCCTGAAGGACATTAACAAAGTGGCCGAGGACCTGGAGTCTGAAGGTCTCATGGCGGAAGAAGT GCAGGCCGTGCAGCAGCAGGAGGTGTATGGTATGATGCCCAGGGATGAAGCAGATTCCAAGACCGCCTCC CCATGGAAGTCTGCTCGACTGATGGTCCACACAGTGGCCACCTTCAACTCCATCAAGGAGCTGAATGAGC GCTGGCGGTCCCTGCAACAGCTGGCTGAGGAACGTAGCCAGCTCCTGGGCAGTGCACACGAAGTACAGAG GTTCCACAGGGATGCGGATGAAACCAAAGAATGGATTGAGGAGAAGAACCAGGCTCTGAACACAGACAAC TATGGCCATGATCTAGCTAGCGTCCAGGCCCTGCAGCGCAAACACGAAGGCTTCGAGAGGGACCTTGCAG CTCTTGGTGACAAGGTGAATTCCCTTGGGGAAACAGCCCAGAGGCTGATCCAGTCCCACCCTGAATCTGC AGAGGACTTAAAGGAAAAGTGCACAGAGTTAAACCAGGCCTGGACCAGCCTAGGGAAGCGTGCAGACCAG CGCAAGGCCAAACTGGGTGACTCCCATGACCTGCAGCGCTTCCTTAGCGATTTCCGGGACCTCATGTCTT GGATCAATGGAATACGAGGGTTGGTATCTTCAGATGAACTGGCCAAGGATGTCACTGGAGCTGAGGCTTT GCTGGAGCGACACCAGGAACACCGGACAGAAATTGATGCCAGGGCTGGCACTTTCCAGGCATTTGAGCAG TTTGGGCAGCAGCTGTTGGCTCATGGGCACTATGCCAGCCCAGAGATCAAGGAGAAACTTGATATTCTTG ACCAGGAGCGCACAGACCTGGAGAAGGCCTGGGTTCAGCGCAGAATGATGCTGGACCACTGCCTGGAGTT GCAGCTGTTCCATCGAGACTGTGAGCAAGCAGAGAACTGGATGGCTGCCCGGGAAGCCTTCCTAAACACA GAAGACAAAGGAGACTCGCTGGACAGTGTGGAGGCTCTGATCAAAAAACATGAAGACTTCGACAAAGCTA TCAATGTCCAGGAGGAGAAGATAGCTGCCCTGCAGGCCTTTGCCGACCAGCTCATTGCTCTGGACCACTA TGCCAAGGGAGACATTGCAAACCGACGCAATGAGGTCCTGGACAGGTGGCGCCGCCTAAAAGCCCAGATG ATTGAGAAAAGGTCAAAGCTCGGAGAATCTCAAACACTTCAGCAGTTCAGCCGGGATGTAGATGAGATTG AAGCTTGGATCAGTGAGAAGTTACAAACAGCCAGCGATGAGTCATACAAGGACCCCACCAACATCCAGAG CAAGCACCAGAAGCACCAAGCCTTTGAGGCAGAACTGCACGCCAATGCTGACCGAATCCGTGGAGTTATT GACATGGGCAACTCCCTCATTGAGCGTGGGGCCTGTGCTGGCAGTGAGGATGCTGTCAAGGCCCGCCTGG CTGCCCTTGCAGACCAGTGGCAGTTCCTGGTGCAGAAGTCAGCTGAGAAGAGCCAGAAGCTGAAAGAGGC CAATAAGCAGCAGAACTTCAACACCGGGATCAAAGACTTTGACTTCTGGCTTTCTGAGGTGGAGGCTCTC CTGGCATCTGAAGACTACGGCAAAGACCTGGCTTCCGTGAACAACCTGCTCAAAAAGCATCAGCTGCTGG AGGCAGACATATCGGCCCACGAGGATCGTCTGAAGGACCTGAACAGCCAGGCTGACAGCCTGATGACTAG CAGTGCCTTCGACACCTCCCAAGTGAAAGAGAAGCGGGACACCATCAATGGACGCTTTCAGAAGATCAAG AGCATGGCAACCTCCCGAAGAGCAAAACTGAGCGAGTCCCATCGCCTGCACCAGTTTTTCCGAGACATGG ATGACGAGGAGTCCTGGATCAAGGAGAAGAAGTTGTTAGTGAGCTCTGAGGACTATGGCAGAGACCTCAC TGGTGTTCAAAATCTGAGGAAGAAACACAAGCGGCTAGAAGCCGAACTGGCCGCACACGAACCAGCCATT CAGGGTGTCCTGGACACGGGGAAGAAGCTGTCTGATGACAACACCATCGGGCAGGAGGAGATCCAGCAGC GTCTCGCACAGTTTGTGGAGCACTGGAAGGAACTGAAACAGCTAGCAGCTGCACGGGGCCAGCGGCTGGA GGAGTCCTTGGAGTATCAGCAGTTTGTGGCCAACGTGGAGGAGGAGGAGGCTTGGATCAATGAGAAGATG ACCCTGGTGGCCAGCGAAGACTACGGGGACACTCTTGCTGCCATCCAGGGCTTACTGAAGAAACATGAAG CATTTGAGACAGACTTCACTGTCCACAAGGATCGAGTGAATGATGTCTGTACTAATGGACAAGACCTCAT TAAGAAGAACAATCACCATGAGGAGAACATCTCTTCAAAGATGAAGGGTCTGAATGGTAAAGTGTCTGAC CTGGAGAAAGCAGCAGCTCAGCGGAAAGCGAACGTGGATGAGAACTCGGCCTTCCTTCAGTTCAATTGGA AGGCTGACGTGGTGGAGTCCTGGATTGGTGAAAAGGAGAACAGCTTGAAAACAGATGATTATGGCCGAGA TCTGTCTTCTGTCCAAACTCTGCTCACCAAGCAGGAGACATTTGATGCTGGCCTGCAGGCCTTCCAGCAG GAGGGCATTGCCAATATCACTGCCCTCAAAGACCAGCTGCTAGCTGCCAAGCACATTCAGTCGAAGGCCA TCGAGGCCCGACATGCCTCCCTCATGAAGAGGTGGACCCAGCTGTTGGCCAATTCAGCTACCCGCAAGAA GAAGTTGCTAGAGGCCCAGAGTCATTTCCGAAAGGTAGAAGACCTCTTCCTGACCTTTGCCAAAAAGGCA TCGGCTTTCAACAGCTGGTTTGAGAATGCAGAAGAGGACCTCACAGACCCAGTGCGCTGCAACTCTCTGG AAGAAATCAAAGCCCTCCGAGAGGCTCATGATGCCTTCCGCTCATCGCTCAGCTCTGCGCAGGCCGACTT CAACCAGCTAGCCGAGCTGGACCGTCAGATCAAGAGTTTCCGAGTGGCCTCCAATCCCTACACCTGGTTC ACCATGGAGGCCCTGGAAGAGACGTGGAGGAACCTACAGAAGATCATTAAGGAGCGAGAACTGGAGCTGC AGAAGGAACAGCGGCGGCAGGAGGAGAATGACAACGTACGCCAAGAGTTTGCCCAGCATGCCAACGCGTT CCACCAGTGGATCCAGGAAACAAGAACGTATCTCCTCGACGGGTCCTGCATGGTCGAAGAGTCGGGAACT CTGGAATCTCAGCTTGAAGCTACCAAACGCAAGCACCAGGAGATTCGGGCCATGAGAAGTCAGCTGAAGA AGATTGAGGACCTGGGTGCTGCCATGGAGGAAGCCCTCATCCTGGACAACAAGTACACTGAGCACAGCAC TGTGGGCCTGGCCCAGCAGTGGGACCAGTTAGACCAGCTGGGCATGCGCATGCAGCACAACCTGGAGCAG CAGATCCAGGCCAGGAACACAACAGGAGTCACTGAGGAGGCCCTCAAGGAGTTCAGCATGATGTTCAAAC ACTTCGACAAGGACAAGTCTGGCCGGCTGAATCATCAAGAGTTCAAATCCTGCCTTCGTTCTCTGGGTTA CGACCTGCCAATGGTTGAGGAAGGAGAGCCTGATCCTGAGTTTGAGGCCATACTGGACACTGTTGATCCC AACAGGGACGGCCACGTCTCCCTGCAAGAGTACATGGCTTTCATGATCAGCCGTGAAACCGAGAATGTCA AGTCCAGTGAAGAGATCGAGAGTGCTTTCCGGGCCCTCAGCTCCGAGGGCAAGCCTTATGTGACCAAGGA GGAGCTCTACCAGAACCTGACCCGGGAACAAGCTGACTACTGTGTCTCCCACATGAAGCCCTATGTGGAT GGCAAGGGCCGCGAACTTCCAACTGCCTTCGACTACGTGGAGTTCACCCGTTCTCTCTTTGTGAATTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_171983
Insert Size	7419 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_171983.2, NP_741984.2
RefSeq Size	7866 bp
RefSeq ORF	7419 bp
Locus ID	64159
UniProt ID	P16086
Gene Summary	a filamentous protein that plays an important role in membrane organization [RGD, Feb 2006]

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