Flii (NM_001008279) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Fliih
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205540 representing NM_001008279 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCACCGGGGTGCTGCCGTTCGTGCGCGGCGTGGACCTCAGCGGCAACGACTTCAAGGGCGGCT ACTTCCCTGAGAATGTCAAGGCTATGACCAGCCTGCGATGGCTAAAGCTGAACCGCACAGGTCTCTGCTA CCTGCCTGAAGAACTAGCAGCTCTGCAGAAGCTGGAGCACCTGTCTGTGAGCCACAACAATCTGACCACG CTTCATGGGGAACTGTCCAGCCTGCCTTCACTTCGGGCCATTGTAGCTCGAGCCAACAGCCTGAAGAATT CTGGAGTCCCCGATGATATCTTCAAGCTGGATGATCTCTCCGTTCTGGACTTGAGCCATAACCAGTTGAC AGAATGCCCACGAGAGCTGGAGAACGCCAAGAACATGCTGGTGCTCAACCTCAGCCATAACAGCATTGAC TCCATCCCCAACCAGCTGTTCATCAACCTTACGGACTTGCTGTACCTGGACCTCAGTGAGAACCGCCTAG AGAGCCTTCCCCCTCAAATGCGCCGCCTCGTGCACCTTCAGACACTGGTGCTGAATGGAAACCCGCTGCT GCATGCACAGCTCCGGCAGCTCCCGGCCATGATGGCCCTACAAACCCTGCATCTGAGGAACACTCAACGC ACCCAGAGCAACCTCCCCACTAGCCTGGAGGGCCTGAGCAACCTTTCAGACGTGGACCTGTCGTGCAATG ACTTGACAAGGGTGCCAGAGTGCCTGTACACCCTCCCCAACCTGCACCGCCTCAACCTCAGTAGCAACCA GATCGCAGAGCTATCTCTGTGCATCGACCAGTGGGTGCACCTGGAGACCTTGAATCTGTCCCGCAACCAG CTCACCTCCCTGCCTTCAGCCATTTGCAAGCTCACGAAGCTGAAGAAATTGTACCTGAACTCCAACAAGC TGGACTTTGATGGGCTACCTTCTGGCATCGGCAAGCTCACCAGCCTGGAGGAATTCATGGCTGCTAACAA CAACCTAGAACTGATTCCAGAAAGTCTCTGCAGGTGCCCAAAGCTGAGGAAACTCGTCCTGAACAAGAAC CGCCTGGTGACCCTTCCTGAGGCCATCCACTTTCTGACAGAGATCGAGGTCCTGGATGTTCGAGAAAACC CCAGCTTGGTCATGCCGCCCAAGCCTGCTGACCACACCGCCGAGTGGTACAACATTGACTTCTCACTACA GAACCAGCTGAGGCTGGCGGGTGCCTCCCCGGCCACAGTGGCCGCCGCTGCAGCTGTAGGGAGCGGGTCC AAGGACCCCTTGGCTCGCAAGATGCGGCTACGGAGGCGCAAGGACTCGGCACAGGATGTTCAGGCCAAGC AAGTGCTAAAGGGCATGTCAGACGTTGCCCAGGAGAAAAACAAAAACCAAGAGGAAAGCACAGATGCCCG AGCACCTGGGGGAAAGGTACGGCGCTGGGACCAGGGCCTGGAGAAGCCACGCCTTGACTACTCGGAGTTC TTTACGGAGGATGTGGGCCAGCTGCCTGGGTTGACCATCTGGCAAATTGAGAACTTTGTGCCTGTGCTGG TGGAGGAAGCCTTCCATGGCAAGTTCTACGAGGCCGACTGCTACATTGTGCTCAAGACCTTCCTGGATGA CAGCGGCTCCCTGAACTGGGAGATCTACTACTGGATTGGCGGGGAGGCCACACTTGACAAGAAAGCCTGT TCTGCCATTCATGCTGTCAACCTGCGCAACTATCTGGGCGCTGAGTGCCGTACTGCGCGGGAGGAGATGG GCGATGAGAGTGAGGGCTTCCTGCAGGTGTTTGACAATGACATCTCCTACATTGAAGGCGGAACAGCCAG TGGCTTCTATACTGTGGAAGACACACACTATGTTACCAGGATGTACCGTGTGTATGGGAAAAAGAACATC AAATTGGAGCCTGTGCCCCTCAAGGGGACCTCACTGGATCCGAGGTTTGTGTTTCTCCTGGACCAAGGAC TGGACATTTATGTATGGAGAGGGGCCCAGGCCACACTGAGCAACACCACAAAGGCCAGACTCTTTGCAGA GAAAATGAACAAGAATGAGCGGAAAGGGAAGGCGGAAATCACGCTCCTGGTGCAGGGTCAGGAGCCACCG GAGTTCTGGGATGTGCTGGGCGGGGAACCCTCTGAGATAAAAAAGCATGTGCCTGATGACTTCTGGCCGC CCCAGCCCAAGCTATACAAGGTGGGCCTGGGGCTGGGCTACTTGGAGCTTCCGCAGATCAACTACAAGCT CTCAGTGGAACACAAAAAACGGCCCAAGGTGGAGCTGATGCCAGGAATGAGGCTGTTGCAGAGCCTGCTG GACACACGATGTGTGTACATCCTGGACTGCTGGTCTGATGTATTCATCTGGCTTGGCCGCAAGTCCCCAC GCCTAGTGCGTGCCGCTGCACTCAAACTGGGCCAGGAGCTGTGTGGGATGCTACACCGGCCACGACATAC AGTGGTCAGCCGCAGCCTGGAGGGCACCGAGGCACAGGTGTTCAAGGCCAAGTTCAAAAACTGGGATGAT GTGTTGACAGTGGACTACACACGAAATGCAGAGGCTGTGCTGCAGGGCCCGGGGATCTCTGGGAAGGTGA AGCGAGACACGGAGAAGAAAGACCAGATGAAGGCTGACCTCACTGCACTCTTCCTACCTCGGCAGCCACC AATGCCACTGGCTGAGGCCGAGCAGCTGATGGAGGAGTGGAACGAAGACCTGGATGGCATGGAGGGCTTC GTGCTGGAGGGCCGGAAGTTCACTCGACTGGCAGAGGAGGAGTTTGGCCACTTCTACACACAGGACTGCT ATGTCTTCCTCTGCAGGTACTGGGTGCCTGTGGAGTACGAGGAGGAAGAGAAGCCAGAAGACAAGGAAGG GAAGGCCTCGGCAGAGGGCAGAGAAGAGGAGGAAGCAGCAGCTGAGACAGAGGAGAAGCAGCCAGAGGAG GATTTTCAGTGCATCGTTTACTTCTGGCAGGGCCGGGAGGCCTCCAACATGGGCTGGCTTACCTTCACAT TCAGTCTGCAGAAGAAGTTCGAGAGCCTTTTCCCTGGCAAGCTAGAGGTAGTACGCATGACACAACAACA GGAGAACCCTAAGTTTCTATCCCACTTCAAAAGAAAGTTCATCATCCACCGGGGCAAGAGGAAGGTGACC CAGACTCTCCAGCCGACCCTCTATCAGATCCGGACTAATGGCAGTGCCCTCTGCACCCGGTGCATTCAGA TCAACACTGACTCCAGTCTTCTCAACTCTGAGTTCTGCTTCATCCTCAAGGTCCCCTTTGAAAGCGAGGA CAACCAAGGCATCGTGTATGCCTGGGTAGGCCGGGCATCAGACCCTGACGAAGCCAAGCTGGCAGAGGAC ATCCTGAACACCATGTTTGACCCCTCCTACAGCAAACAGGTCATCAACGAGGGTGAGGAGCCAGAGAACT TCTTCTGGGTAGGCATCGGTGCACAGAAACCCTATGACGATGACGCAGAGTACATGAAGCACACGAGGCT CTTCAGGTGCTCCAATGAGAAGGGCTACTTCGCAGTGACTGAGAAATGCTCTGACTTTTGCCAAGATGAC CTGGCAGACGATGACATCATGCTGCTAGACAATGGCCAAGAAGTGTACATGTGGGTTGGGACTCAGACAA GCCAAGTGGAGATCAAACTGAGTCTGAAGGCTTGCCAGGTATACATCCAGCACACACGCTCTAAAGAACA TGAGCGGCCACGCCGCCTGCGCCTGGTCCGCAAAGGTAACGAACAACGTGCCTTCACCCGCTGCTTCCAT GCCTGGAGCACGTTCCGTCAAGCCCCAGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001008279
Insert Size	3813 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001008279.1, NP_001008280.1
RefSeq Size	4035 bp
RefSeq ORF	3813 bp
Locus ID	287375

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