Rbm20 (NM_001107611) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205470 representing NM_001107611 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGGCTGCAGCCATGAGCCAAGACGCGGATCCCAGCGGTCCGGAGCAACCCGACAGAGATGCCT GCATTGTGCCTGGTGTTCAAGGGCCCCCTGCGCCCCAAGGCCAGCAAGGGATGCAGCCCCTGCCGCCACC GCTACCGCCACCGCCTCAGCCTCAATCCAGCCTGCCCCAGATCATCCAAAATGCTGCCAAGCTCCTGGAC AAGAACCCTTTCTCCGTCAGTAGCCAGAACCCTCTGCTCACTTCGCCAGCCAGCGTCCAGCTGGCCCAGA TACAGGCCCAGCTCACCCTCCATCGGCTGAAGATGGCACAGACCGCAGTCACCAACAACACTGCAGCCGC CACTGTCCTCAACCAAGTCCTCTCCAAAGTGGCCATGTCCCAGCCTCTCTTCAACCAGCTTCGCCATCCG TCTGTGCTTGGCACCACACATGGCCCTACTGGGGTCTCCCAGCATGCTGCCACCGTTCCCAGTGCTCACT TTCCCTCAACTGCAATCGCCTTTTCGCCCCCAAGCCAGGCAGGAGGCCCGGGGCCTTCTGTGAGCCTCCC CAGCCAGCCCCCCAATGCTATGGTAGTGCATACCTTCAGTGGGGTGGTGCCTCAGACCCCTGCCCAGCCA GCAGTCATCCTAAGCATTGGGAAGGCGGGGCCGACACCTGCTACTACAGGGTTCTATGACTATGGCAAAG CCAACCCTGGCCAGGCCTATGGTTCTGAAACGGAGGGCCAGCCGGGCTTCTTGCCAGCCTCGGCGTCAGC CGCAGCATCAGGCGGTGTGACCTATGAAGGACACTATAGCCACACAGGGCAGGATGGCCAAGCCACCTTT TCTAAAGACTTCTATGGACCCAGTGCCCAAGGGTCACATGCAGCAGGTGGGTTCCCAGCTGATCAGGCTG GGAGCATGAAAGGAGACGTCGGTGGGTTGTTGCAAGGTACCAACAGCCAGTGGGAGAGGCCCTCTGGGTT CTCTGGCCAGAACAAGGCCGATATTACAGCCGGGCCCGGTTTGTGGGCTCCACCTGCCAGTCAGCCTTAT GAACTATATGATCCTGAGGAGCCTACCTCAGACAGGGCCCCTCCTGCCTTTGGATCTCGACTTAACAACA GCAAGCAGGGATTCAACTGCTCCTGCCGGCGGACAAAGGAGGGGCAGGCCATGCTGTCCGTGAGGCCCCT GCAGGGTCATCAACTGAATGACTTCCGAGGCTTGGCTCCACTCCACCTTCCACATATCTGCAGCATCTGT GACAAGAAGGTGTTTGACCTGAAGGACTGGGAGCTACATGTGAAGGGGAAGTTGCATGCCCAGAAATGCC TGCTCTTCTCAGAAAGTGCTGGCCTCCGGAGTATATGTGCTACAGGAGAAGGGACGCTGTCTGCCTCTGC AAACAGCACAGCTGTTTATAACCCCACTGGAAATGAGGATTATACCTCAACTCTTGGAACATCATATGCA GCCATTCCAACAAGGGCCTTTGCTCAATCAAACCCCATGTTTCCTTCGGCTTCCTCTGGGACAAATTTTG CACAGAGGAAAGGTGCTGGACGAGTTGTGCACATCTGCAATCTCCCAGAGGGCAGCTGCACGGAGAATGA CGTCATCAACCTGGGGCTGCCCTTTGGCAAGGTCACTAATTACATCCTTATGAAGTCAACTAATCAGGCT TTCTTGGAGATGGCTTACACAGAAGCTGCTCAAGCTATGGTCCAGTACTACCAAGAAAAGCCCGCGCTTA TCAATGGCGAGAAGTTACTCATTCGGATGTCCACGAGATACAAGGAATTGCAGCTGAAGAAACCTGGGAA AAATGTGGCTGCTATCATCCAGGACATCCACTCCCAAAGGGAGAGGTGCATGCTCCGGGAAGCTGACAGA TATGGTCCAGAGCGACCACGTTCTCGAAGTCCAATGAGCCGATCGCTCTCCCCGAGATCCCACAGTCCTC CTGGCCCCTCTCGGGCTGATTGGGGCAATGGCCGTGACTCCTACGCATGGAGGGACGAGGATCGGGAGAC GGTGCCCAGGAGGGAGAACGGGGAAGACAAGAGGGACAGGTTGGATGTTTGGGCACATGACCGGAAACAC TATCCCAGGCAGCTGGACAAAGCCGAGTTGGATGAGCGACTCGAAGGAGGGAGGGGCTACCGGGAAAAGT ACTTGAAGTCAGGGTCCCCCGGCCCACTCCATTCTGCGTCTGGCTACAAAGGCCGGGAAGATGGCTACCA TCGAAAAGAGACTAAAGCTAAGTTGGACAAACACCCAAAGCAGCAGCAGCAGGATGTGCCAGGAAGATCC AGGAGGAAAGAAGAGGCGCGACTACGGGAGCCCAGACACCCTCACCCAGAGGACTCTGGCAAGGAAGAGG ATCTGGAGCCCAAGGTCACTCGGGCCCCCGAGGGTACCAAGTCCAAGCAAAGTGAGAAAAGTAAAACCAA GAGAGCCGACAGAGACCAAGAAGGAGCTGATGACAAAAAAGAAGGCCGAGGGGCAGAGAATGAGGCTGGG ACTGAGGAACAGGAAGGCATGGAAGAGAGCCCCGCATCGGTGGGCACACAGCAGGAAGGGACGGAGTCCT CCGATCCAGAAAACACAAGGACAAAGAAGGGACAAGACTGTGACAGTGGAAGTGAGCCTGAGGGGGACAA CTGGTACCCCACCAACATGGAGGAGCTGGTCACAGTGGACGAAGTAGGGGAGGAAGACTTCATCATGGAG CCAGACATACCGGAGCTGGAAGAAATTGTACCCATCGACCAGAAAGACAAAATCCTCCCCGAAATATGTC CCTGTGTAACAGCCACCTTAGGTTTGGACTTGGCCAAAGACTTCACCAAGCAGGGAGAGACCCTAGGGAA CGGAGACGCAGAACTCAGCCCGAAGCTGCCCGGACAAGTGCCGTCTACTTCCACAAGCTGTCCCAATGAC ACGGACATGGAGATGGCTGGCCTAAATCTGGATGCTGAGCGGAAGCCAGCTGAAAGCGAGACAGGCCTCT CACCGGAGGTCTCAAATTGCTACGAGAAGGAGGCGAGAGGAGCGGAGGGCTCAGATGTGCGTCTGGCCCC TGCAGCCCAGCGAATGTCCTCCCCTCAGCCAGCAGATGAGAGGGCCCGGCAGTCCAGCCCTTTTCTCGAT GACTGCAAGGCCAGGGGGTCCCCAGAAGATGGGCCTCATGAAGTCAGCCCCCTGGAAGAAAAAGCCAGCC CCACTACTGAAAGCGACCTCCAAAGCCAGGCTTGCCAAGAAAACTCCAGGTACACGGAGACGAGATCTCT GAACTCGAGATCACCAGAATTCACCGAAGCGGAGCTGAAAGAGCCCCTTTCTTTGCCTTCCTGGGAACCA GAGGTGTTCAGTGAACTTAGCATTCCTCTAGGAGTGGAGTTCGTGGTTCCCAGGACTGGCTTTTACTGCA AGCTGTGTGGCCTGTTCTACACAAGCGAGGAGGCGGCCAAAGTGAGCCACTGCCGCAGCACTGTACACTA CAGGAACTTACAGAAGTACCTGTCCCAGCTGGCAGAGGAGGGACTGAAGGAGACAGAGGGGGTAGACAGC CCAAGCCCCGAGCGCAGCGGGATTGGTCCACACTTGGAAAGGAAGAAGCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107611
Insert Size	3624 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107611.2, NP_001101081.2
RefSeq Size	6682 bp
RefSeq ORF	3624 bp
Locus ID	309544
UniProt ID	E9PT37
Gene Summary	RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin).[UniProtKB/Swiss-Prot Function]

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