Per2 (NM_031678) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	rPER2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205432 representing NM_031678 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGATATGTGGACTTTTCCCCAAGTCCCACCAGCCCCACCCAAGAGCCAGGGGAGCCTCAACCCA CCCAGGCTGTGCTCCAGGAAGACGTGGACATGAGCAGCGGCTCCAGCGGAAATGAAAACTGCTCCACGGG GCGGGACTCTCAGGGCAGTGACTGTGACGACAGTGGAAAGGAGCTGCGGATGTTAGTGGAATCGTCCAAC ACTCACCCCAGCCCTGACGATACCTTCAGACTCATGATGACAGAGGCGGAGCATAACCCCTCCACAAGCG GCTGCAGTAGTGAGCAGTCTGCCAAAGCTGACGCACACAAAGAGCTGATAAGGACCCTGAGGGAGCTGAA GGTCCACCTCCCTGCAGACAAGAAGGCCAAGGGGAAGGCCAGCACGCTGGCAACCTTGAAGTACGCTCTG CGGAGCGTGAAGCAGGTGAAGGCTAATGAGGAGTACTACCAGCTGCTAATGTCCAGTGAGAGCCAGCCCT GCAGCGTGGATGTGCCTTCCTACACCATGGAGCAGGTTGAGGGCATTACCTCCGAGTATATTGTGAAGAA CTCGGACATGTTTGCTGTGGCTGTGTCCCTGGTCTCTGGGAAGATCCTGTACATCTCCAACCAAGTCGCC CCCATCTTTCACTGTAAGAAGGACGCCTTCAGTGATGCCAAGTTTGTGGAGTTCCTGGCTCCCCATGACG TCAGTGTGTTCCACAGCTACACCACCCCTTACAAGCTTCCGCCCTGGAGTGTGAGCAGTGGCTTAGATTC TTTCACTCAGGAGTGCATGGAGGAGAAATCTTTTTTCTGCCGTGTCAGTGTTGGGAAACACCACGAGAAT GAGATTCGCTACCAGCCCTTCCGCATGACACCCTACCTGGTCAAGGTGCAAGAGCAGAAGGGCGCTGCGA GCCAGCTCTGCTGCCTGCTGCTAGCAGAGAGGGTACACTCAGGCTATGAAGCTCCTAGAATTCCTCCCGA GAAGAGAATTTTCACAACAACCCACACACCAAACTGCCTGTTCCAGGATGTGGACGAAAGGGCGGTCCCC CTCCTGGGCTATCTACCTCAGGATCTGATCGAGACCCCTGTGCTCGTGCAGCTCCACCCCAGCGACCGGC CCTTGATGCTCGCCATCCACAAGAAGATCCTACAGGCCAGTGGGCAGCCTTTCGATTATTCTCCCATTCG ATTCCGCACACGCAACGGGGAGTACATCACACTGGACACTAGCTGGTCCAGCTTCATCAACCCGTGGAGC AGGAAGATATCCTTCATCATCGGGAGGCACAAAGTCAGGGTAGGCCCTTTGAATGAGGATGTGTTCGCAG CCTCCCCTTGCCCAGAGGAGAAGACTCCGCACCCCAGCGTTCAGGAGCTCACAGAGCAAATCCACCGGCT ACTGATGCAACCTGTCCCCCACAGCGGCTCCAGTGGCTATGGGAGCCTGGGCAGTAACGGATCCCACGAA CACCTCATGAGCCAGACATCATCCAGCGACAGCAATGGTCAAGAGGAGTCTCACTGGAGGAGATCCGGAA TTTTTAAAACCAGTGGCAAGAGTCAAAGCAAAAGTCACTTTTCTCCTGAGTCTGGAGGACAAAAGGAAGC CTCTGTTGCAGAAATGCAAAGTAGTCCTCCAGCTCAGGTGAGGTCTGTCACCACCATGGAAAGGGACAGC TCGGGGGCCAGCCTACCCAAGGCTAGCTTTCCAGAGGAACTAACCTATAAGAGCCAGCCTCCTTGCTCCT ACCAGCAGATCAGCTGCCTGGACAGTGTCATCAGGTACCTGGAGAGCTGCAACGAGGCAGCCACCCTGAA AAGAAAGTGCGAGTTCCCAGCCAACATCCCATCCCGGAAGGCCACGGTCAGCCCTGGGCTGCACTCTGGA GAGGCAGCGCGGTCCTCCAAGGTGACCAGCCACACGGAGGTCAGTGCTCACTTGAGCTCCTTGGCGTTGC CGGGCAAGGCCGAGAGTGTGGTGTCCCTCACCAGCCAGTGCAGCTACAGCAGCACCATCGTGCACGTGGG CGACAAAAAGCCACAGCCTGAACTAGAGACAGTAGAAGATGTGGCCAGTGGGCCTGAGTCCCAGGATGAT GCAGCTGGTGGCCTCAGCCAAGAAAAGGGGTCTCTGCAGAAGCTAGGCCTCACCAAGGAAGTTCTGGCTG CACATACCCAGAGAGAGGAACAGGGCTTCCTGCAGAGGTTCAGGGAAGTGAGCAGGCTCGGTGCCCTGCA GGCTCACTGCCAGAACTATCTCCAGGAGCGGTCCCGAGCCCCAGCAAGTGATCGAGGACTAAGAAATGCT TCTGGAATAGAATCATCTTGGAAAAAAACTGGAAAGAACAGGAAACTGAAGTCCAAGCGTGTCAAGACTC GAGACTCTTCTGAGAGCACAGGGTCTGGGGGACCCGTGTCCCACCGACCTCCCCTCGTGGGCCTGAATGC CACAGCCTGGTCGCCCTCTGACACATCCCAGTCTAGCTGTCCCTCTGCACCATTCCCTGCTCCAGTGCCA GCTTACCCACTACCTGTGTTCCCGGCACCTGGAATAGTATCCACACCAGGGACGGTGGTGGCACCACCTG CAGCCGCCCACACCGGCTTCACCATGCCTGTTGTGCCTATGGGCACCCAGCCTGAATTCGCAGTGCAGCC CCTGCCGTTCGCTGCCCCCTTGGCTCCGGTCATGGCCTTCATGCTACCCAGCTACCCGTTTCCACCAGCA ACCCCAAACCTGCCTCAGGCCTTCTTCCCCAGCCAGCCTCACTTTCCGGCCCATCCCACACTTGCCTCTG AAATAACTCCTGCCTCCCAGGCTGAGTTCCCTAGTCGGACCTCGATGCTCAGGCAGCCATGTGCTTGCCC AGTCACCCCCCCGGCTGGCACAGTGGCCTTGGGCAGAGCCTCCCCGCCACTCTTCCAGTCCCGAGGCAGC AGTCCCCTACAGCTTAACCTGCTTCAGCTAGAGGAAGCACCTGAAAGTAGTACTGGAGCTGCAGGGACCT TGGGGACCACGGGGACAGCAGCTTCTGGTCTGGACTGCACATCTGGCGCATCTCGGGACCGGCAGCCAAA GGCACCTCCAACATGCAGTGAGCCCTCAGACACCCAGAACAGTGATGCCATCTCCACCTCCAGTGACCTG CTCAACCTCCTCCTGGGCGAGGACCTCTGCTCAGCCACCGGCTCAGCACTGTCGAGAAGCGGGGCATCTG CCACCTCAGACTCACTGGGCTCCAGCTCCCTGGGCTGTGACACATCCCGGAGTGGGGCAGGCAGCAGTGA TACAAGTCACACCAGCAAATACTTTGGAAGCATTGACTCTTCAGAGAATAATCACAAAGCAAAAATGATC ACAGACACGGAGGAGAGTGAACAGTTCATTAAGTACGTCTTGCAGGACCCCATCTGGCTGCTGATGGCCA ACACAGACGACAATATCATGATGACATACCAGCTGCCCTCCCGGGATCTCCAGGCGGTCTTGAAAGAGGA CCAGGAGAAGCTGAAGCTGCTGCAGAGGTCCCAGCCCCACTTCACGGAGGGCCAGAGGCGAGAGCTTCGA GAGGTTCATCCGTGGGTCCACACCGGGGGTCTGCCTACCGCCATCGACGTAACAGGGTGTGTTTACTGTG AAAGTGAGGAGAAAGGCAACCTTTGTCTGCCATATGAGGAAGACAGTCCTTCCCTGGGACTCTGTGATAC CTCAGAAGCCAAAGAGGAGGAGAGCGGACAGCTGGCCAATCCTAGGAAGGAGGCCCAGACGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031678
Insert Size	3774 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031678.1, NP_113866.1
RefSeq Size	5761 bp
RefSeq ORF	3774 bp
Locus ID	63840
UniProt ID	Q9Z301
Gene Summary	This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014]

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