Pitpnm1 (NM_001008369) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pitpnm
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205416 representing NM_001008369 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTATCAAGGAGTACCACATCTTGCTGCCCATGAGCCTGGACGAGTACCAAGTGGCCCAGCTCTACA TGATCCAGAAAAAGAGCCGGGAGGAATCTAGTGGTGAGGGCAGTGGTGTGGAGATCCTGGCCAACCGGCC CTACACAGATGGACCCGGAGGCAATGGGCAGTACACACACAAGGTGTACCACGTGGGCTCCCACATCCCA GGCTGGTTCCGGGCACTGTTGCCCAAGGCTGCTCTGCAGGTAGAAGAGGAATCTTGGAATGCTTACCCGT ACACCCGGACACGGTACACCTGCCCCTTTGTGGAGAAATTCTCCATTGAGATAGAGACCTATTACTTGCC TGATGGGGGGCAGCAGCCTAACGTCTTCAACTTGAGCGGGGCGGAGAGAAGACAGAGAATCCTGGATACC ATCGACATCGTGCGGGATGCAGTGGCCCCAGGAGAGTACAAAGCAGAAGAGGACCCTCGGCTGTACCGCT CAGTCAAGACAGGCCGAGGGCCACTGGCTGATGACTGGGCACGGACAGCAGCCCAGACAGGACCTCTCAT GTGTGCCTATAAGCTGTGCAAGGTTGAATTCCGCTACTGGGGCATGCAGGCCAAGATTGAGCAGTTCATC CATGATGTAGGTCTGCGCAGGGTAATGCTTCGGGCCCATCGCCAGGCCTGGTGTTGGCAGGATGAGTGGA TTGAACTGAGCATGGCTGACATCCGGGCACTGGAAGAGGAGACAGCACGTATGCTGGCCCAGCGTATGGC TAAGTGCAACACTGGCAGTGAGGGACCGGAGGCCCAGACCCCTGGGAAACCCAGCACTGAGACCCAGCCT GGGACCAGAACGGCTGGCACCCCTGATGGGCCTGAGGCCCCTCCTGGCCCCGACGCCTCACCAGATGCCA GCTTTGGGAAGCAGTGGTCCTCATCTTCCCGTTCCTCCTACTCATCCCAACATGGAGGTGGTGTGTCTCC ACAGAGCTTGTCTGAGTGGCGCATGCAGAACATTGCCCGGGACTCTGAGAACAGCTCCGAGGAAGAATTC TTTGATGCCCATGAAGGTTTCTCAGACAGTGATGAGGTCTTCCCCAAGGAGATGACCAAGTGGAACTCCA ATGATTTTATTGATGCCTTTGCCTCCCCGACTGAGGTAGAGGGGGTGCCAGATCCTGCAATCATGGCTAC CAAAGGCATTGAAGATGAGGCCCGAGCTCCCAGGGACTCAGAGGGCCTGGATGGAACCGGGGATCTAGGG GTTGAGGCATGCTCTGTGCATGCCCTCTTCCTCATCCTACACAGCGGCAGCATCCTGGACTCTGGCCCTG GAGACACCAACTCCAAGCAGGCCGATGTTCAAACACTGAGCACAGCCTTTGAGGCCGTCACCCGAGTCCA TTTCCCCGAGGCCCTGGGTCACGTGGCACTGCGGCTGGTGCCCTGTCCACCCATCTGCGCGGCTGCCTAT GCTCTTGTCTCCAACCTGAGCCCCTACAGCCATGATGGGGACAGCCTGTCCCGCTCCCAGGACCACATTC CACTGGCTGCTCTGCCACTGCTGGCCACCTCATCCTCCCGCTACCAGGGTGCCGTGGCCACTGTCATCGC TCGCACCAACCAGGCCTATGCAGCCTTCCTGCGCTCATCGGAGGGCACAGGTTTCTGCGGGCAGGTGGTG CTGATCGGAGATGGTGTTGGTGGCATCCTGGGCTTTGATGCGCTCTGCCACAGTGCCAGTGCAGGCACAG GGAGTCGAGGCAGCAGCCGCCGTGGGAGCATGAACAATGAGATGCTCTCCCCAGAAGTTGGTCCAGTACG GGACCCGCTGGCAGATGGGGTGGAGGTACTGGGTCGTGCTAGCCCAGAACCCTCAGCCCTACCTGCTCAG CGCACCTCCAGCGACATGGCTAACCCGGATCCTGATGGCTCTCAGAACAGCCTACAGGTAGCCCCCACAG TTACCTCTGGGGAGCCCCGTCGTGCAAGCACAGCCTCCTGTCCACCTGCCAGTTCTGAGGCTCCTGACGG CCCCACTAATGCTGCTCGACTGGACTTCAAGGTCTCAGGCTTCTTCCTCTTTGGCTCCCCACTGGGCTTG GTGCTGGCTCTACGCAAAACCGTCATGCCTGCCTTGGAGGTGGCTCAGATGCGTCCAGCCTGCGAACAGA TCTACAATCTCTTCCATGCCGCTGACCCCTGTGCCTCCCGCCTGGAACCCCTGCTGGCCCCCAAGTTCCA GGCCATCGCCCCCCTGGCTGTACCTCGTTACCAGAAGTTTCCCCTAGGAGATGGCTCATCCCTGCTGCTG GCTGACACTTTGCAGACCCATTCGAGCCTCTTCCTGGAAGAGCTGGAGATGATGGTGCCCTCCACGCCCA CCTCGGCCAGTGGTGCCTTCTGGAAGGGCAATGAATTAGGCAATGAGCCGGCAGCCCAGCCAGCAGCCCC CAGCACCACCAGTGAGGTGGTTAAGATCCTGGATCGCTGGTGGGGAAACAAGCGCATCGACTATTCACTG TACTGCCCTGAGGCACTCACCGCCTTTCCCACCGTCACGCTGCCCCACCTCTTCCACGCCAGCTACTGGG AGTCGGCAGACGTAGTGGCCTTCATTCTGCGCCAGGTCATTGAGAAGGAGCGGCCACAGTTGACAGAATG TGAGGAGCCATCCATCTACAGCCCTGCCTTCCCCAGGGAGAAGTGGCAGCGCAAACGCACACAGGTCAAG ATCCGGAACGTCACTTCCAACCATCGGGCAAGTGACACCGTGGTATGCGAGGGCCGTCCCCAGGTGCTGA ATGGGCGCTTCATGTACGGACCACTGGACGTGGTCACACTCACTGGCGAGAAGGTGGATGTCTATGTCAT GACACAGCCCCTGTCGGGCAAGTGGATCCACTTTGGCACAGAGGTCACCAACAGCTCAGGCCGTCTCACC TTCCCAGTGCCCTCAGAACGTGCACTGGGCATTGGTGTCTACCCTGTGCGCATGGTGGTCAGGGGAGACC ATACCTACGCCGAGTGCTGTCTGACTGTGGTGTCCCGAGGCACAGAAGCTGTGGTCTTCAGCATCGACGG CTCATTCACTGCCAGTGTCTCTATCATGGGCAGTGACCCCAAGGTGCGCGCTGGCGCAGTGGACGTGGTC AGGCACTGGCAGGACTCAGGCTACTTGATCGTGTATGTAACTGGCCGGCCTGACATGCAGAAGCACCGTG TAGTGGCCTGGCTGTCGCAACACAACTTCCCCCATGGTGTTGTCTCCTTCTGTGATGGCCTCACCCATGA CCCACTGCGACAGAAAGCTATGTTTCTGCAGAGCCTGGTGCAAGAGGTAGAACTGAACATCGTGGCTGGG TATGGGTCACCGAAAGACGTTGCAGTATATGCGGCCCTGGGACTCTCTCCAAGCCAGACCTACATTGTTG GTCGTGCAGTGCGTAAACTGCAGGCACAGTGTCAGTTCCTGTCAGATGGCTATGTGGCCCATTTGGGCCA GCTGGAGGCAGGCTCCCACTCCCATGCCCCCTCAGGACCTCCAAGAGCTGCTCTGGCCAAGAGCAGCTAT GCTGTGGCTGCCCCTGTGGACTTCCTCCGTAAGCAGAGCCAGCTGCTTCGATCCAGAGGCCCCAGCCAGG TGGACCTCGAGGGCCCAGGAACACCTCCTACCACCCTGGCCAGGGGCAAGACTCGAAGCATCAGCCTCAA GCTAGACAGTGAAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001008369
Insert Size	3729 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001008369.1, NP_001008370.1
RefSeq Size	4191 bp
RefSeq ORF	3729 bp
Locus ID	361694
UniProt ID	Q5U2N3
Gene Summary	Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes. Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions (By similarity).[UniProtKB/Swiss-Prot Function]

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