Robo1 (NM_022188) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205390 representing NM_022188 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATGGAAACATCTTCCTCTTCTGGTCATGATATCTCTCCTCACTCTATCAAAAAAGCATCTCCTTC TGGCCCAGCTTATTCCAGACCCTGAGGACCTAGAGAGGGGGAACGACAACGGGACGCCAGCCCCCACCTC TGATAATGATGACAATTCGCTGGGCTACACAGGCTCCCGTCTCCGTCAGGAAGATTTCCCACCGCGCATT GTTGAGCACCCTTCAGACCTGATCGTCTCTAAAGGGGAACCCGCCACCCTCAACTGTAAAGCTGAAGGCC GCCCTACCCCGACTATAGAATGGTACAAGGGCGGCGAAAGAGTGGAAACGGACAAAGACGACCCTCGCTC ACACCGAATGCTGCTGCCAAGTGGATCTTTATTTTTCTTACGCATAGTGCATGGACGGAAGAGTAGGCCT GACGAAGGGGTCTACATCTGTGTAGCAAGGAATTACCTCGGAGAGGCTGTGAGCCACAATGCATCGCTGG AGGTAGCTATACTACGGGATGACTTCAGACAAAACCCTTCAGATGTTATGGTGGCGGTGGGCGAGCCTGC AGTAATGGAATGCCAGCCTCCTCGGGGCCACCCAGAGCCCACCATCTCCTGGAAAAAGGATGGCTCACCC CTGGATGATAAAGACGAGCGAATCACTATCCGAGGCGGGAAGCTCATGATCACGTACACCCGTAAAAGCG ATGCTGGCAAGTATGTTTGTGTTGGTACCAACATGGTAGGGGAGCGGGAGAGCAAAGTCGCGGACGTGAC TGTCTTAGAGAGACCGTCCTTTGTAAAGAGACCCAGTAATTTGGCAGTGACTGTGGATGACAGTGCTGAG TTTAAGTGTGAGGCCCGAGGAGACCCCGTGCCTACGTTCGGGTGGAGGAAAGATGACGGAGAGCTGCCCA AATCCAGATATGAGATCCGAGATGATCATACTTTGAAAATTCGGAAGGTGACAGCTGGCGACATGGGATC ATACACTTGTGTTGCAGAAAATATGGTTGGGAAAGCCGAAGCATCAGCCACTTTGACAGTTCAAGAGCCA CCACATTTTGTCGTGAAGCCTCGAGACCAGGTGGTTGCCTTGGGGCGAACTGTGACTTTTCAGTGTGAAG CAACTGGAAATCCTCAACCAGCTATCTTTTGGCGGAGAGAGGGGAGTCAGAATCTGCTTTTCTCATACCA GCCTCCACAGTCCTCTAGCCGGTTTTCAGTTTCCCAGACGGGGGACCTCACTGTTACGAACGTCCAGAGG TCTGATGTCGGTTACTATATCTGTCAGACTTTGAACGTGGCTGGAAGCATCATCACAAAGGCATATTTGG AAGTTACTGATGTGATTGCGGACCGGCCTCCCCCAGTCATCCGACAAGGTCCTGTGAATCAGACTGTAGC AGTGGATGGTACTTTAACTCTCAGCTGTGTGGCCACAGGCAGTCCCGTGCCCACAATTCTGTGGAGGAAG GATGGAGTCCTTGTTTCAACCCAAGATTCTCGGATCAAACAGCTGGAGAGCGGGGTCCTGCAGATCCGCT ACGCTAAGCTGGGTGACACCGGTCGGTACACCTGCACTGCTTCGACCCCCAGTGGTGAGGCCACGTGGAG CGCTTACATCGAAGTCCAGGAATTTGGAGTTCCAGTTCAACCTCCAAGACCCACCGACCCCAATTTAATC CCCAGTGCGCCTTCAAAGCCTGAAGTCACAGATGTGAGCAAAAACACAGTCACTTTATTGTGGCAGCCTA ACCTGAACTCAGGAGCAACCCCAACGTCTTATATTATAGAAGCCTTCAGCCATGCATCTGGTAGCAGCTG GCAGACCGTGGCAGAAAATGTGAAAACAGAAACATTCGCCATCAAAGGGCTCAAACCTAATGCGATCTAC CTTTTCCTGGTGAGGGCAGCAAATGCCTATGGGATTAGCGATCCAAGCCAAATATCCGATCCAGTGAAAA CACAAGATGTCCCACCAACCACCCAAGGGGTGGACCACAAGCAGGTCCAGAGAGAACTGGGCAATGTTGT CTTGCATCTCCACAACCCTACCATCCTTTCTTCCTCTTCTGTCGAAGTGCACTGGACGGTGGACCAACAA TCTCAATATATTCAAGGTTATAAAATTCTCTACCGACCATCTGGAGCCAGCCATGGCGAATCGGAGTGGT TAGTTTTTGAAGTGAGGACCCCAACCAAAAATAGTGTGGTGATCCCCGATCTGAGAAAAGGCGTCAACTA CGAGATTAAGGCCCGCCCATTTTTTAATGAGTTCCAAGGAGCAGATAGTGAAATCAAATTTGCCAAAACC TTAGAAGAACGACCAAGTGCCCCTCCCCGAAGTGTCACTGTATCCAAGAATGATGGAAATGGGACAGCAA TTCTGGTCACTTGGCAACCGCCTCCTGAAGACACACAGAATGGAATGGTCCAAGAATATAAGGTTTGGTG TCTCGGGAATGAGACTAGGTATCACATAAACAAGACAGTAGATGGCTCTACCTTTTCGGTGGTCATTCCC TTTCTTGTTCCTGGAATCCGCTACAGTGTAGAAGTGGCTGCAAGCACTGGGGCTGGCCCTGGGGTGAAGA GTGAGCCCCAGTTCATCCAGTTGGATTCCCATGGAAACCCCGTGTCTCCCGAGGACCAAGTGAGCCTGGC TCAGCAGATCTCCGACGTGGTGAAGCAGCCAGCCTTCATTGCGGGCATCGGGGCCGCCTGTTGGATCATT CTCATGGTCTTCAGTATCTGGCTTTACCGACACCGCAAGAAGAGAAACGGGCTCAGCAGCACGTACGCGG GGATCCGGAAAGTCCCGTCCTTTACCTTCACACCAACAGTAACTTATCAAAGAGGAGGCGAAGCCGTCAG CAGTGGAGGGAGGCCGGGACTCCTCAACATCAGTGAACCTGCCACACAGCCATGGCTGGCGGACACGTGG CCCAACACAGGCAACAGTCACAATGACTGCTCCATCAACTGCTGCACAGCCAGCAACGGCAACAGCGACA GCAACCTGACCACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAACAAACA AACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAAAATCAATGAGATG AAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAATCCATCAGGGCAGCCCACTCCCTACG CCACCACGCAGCTCATCCAGGCAAATCTCATCAACAATATGAATAACGGCGGCGGGGACTCCAGCGAGAA ACACTGGAAGCCTCCGGGGCAGCAGAAACAGGAAGTGGCACCAATTCAGTATAACATCATGGAGCAAAAC AAGTTGAACAAAGATTATCGGGCAAATGACACAATCCTTCCAACCATCCCGTACAACCATTCNTATGACC AGAATACAGGAGGATCCTACAACAGCTCAGATCGGGGCAGTAGTACATCTGGGAGTCAAGGCCACAAGAA AGGGGCACGGACACCAAAGGCACCCAAGCAGGGTGGCATGAACTGGGCAGACTTGCTGCCCCCTCCCCCA GCGCATCCTCCCCCACACAGCAACAGCGAGGAATACAGCATGTCTGTAGATGAAAGCTATGATCAAGAAA TGCCGTGCCCTGTTCCGCCTGCACGGATGTATTTGCAACAGGATGAATTAGAAGAAGAGGAAGCTGAAAG AGGCCCCACGCCCCCTGTCCGGGGGGCAGCTTCGTCCCCAGCTGCTGTGTCCTACAGCCATCAGTCCACG GCCACTCTCACCCCGTCTCCCCAGGAAGAGCTCCAGCCTATGTTACAGGATTGTCCGGAGGACCTCGGCC ACATGCCCCACCCACCAGACAGGAGGCGGCAGCCGGTGAGTCCTCCTCCACCACCACGGCCAATTTCCCC ACCACATACTTACGGCTACATTTCAGGACCCCTGGTCTCAGACATGGATACGGATGCCCCAGAAGAGGAG GAAGACGAAGCTGACATGGAAGTTGCTAAGATGCAGACCCGAAGGCTGTTGTTACGTGGGCTTGAACAGA CCCCAGCATCTAGTGTTGGGGACCTCGAGAGCTCGGTCACTGGCTCCATGATCAATGGCTGGGGCTCAGC CTCAGAAGAGGACAACATTTCCAGTGGGCGCTCCAGTGTCAGTTCATCGGATGGTTCCTTTTTCACTGAC GCGGATTTTGCCCAGGCAGTTGCAGCAGCCGCCGAGTATGCGGGCCTGAAGGTGGCTCGCCGCCAGATGC AGGATGCTGCTGGCCGCCGACACTTCCATGCCTCCCAGTGCCCTAGGCCCACGAGTCCTGTGTCTACAGA CAGCAACATGAGTGCTGCTGTAATCCAGAAAGCCAGACCCACCAAGAAACAGAAGCACCAGCCAGGACAT CTGCGCAGAGAGGCCTACACAGATGATCTTCCACCACCTCCAGTGCCACCGCCTGCTATAAAATCGCCCA GTGTCCAGTCCAAGGCACAGCTGGAGGCACGGCCTATAATGGGGCCAAAGCTCGCTTCTATAGAAGCGAG AGCAGACAGATCATCAGACAGAAAAGGCGGCAGTTACAAGGGGAGAGAAGCTCTGGACGGAAGACAAGTC ACTGACCTGCGAACAAGCCCAGGTGACCCCAGAGAAGCACAGGAGCAGCCAAACGAAGGGAAAGCACGAG GGACGAAGACAGCAAAGCGAGACCTTCCACCAGCGAAGACACACCTCATCCCAGAGGATATCCTTCCGTA CTGTAGGCCTACTTTCCCAACGTCAAACAACCCTAGGGATCCCAGTTCTTCAAGCTCAATGTCATCAAGA GGCTCAGGAAGCCGACAGAGAGAGCAAGCAAATGTGGGCCGAAGAAATATGGCAGAAATGCAAGTACTTG GGGGATTTGAAAGAGGAGATGAGAATAATGAAGAATTAGAGGAAACTGAAAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022188
Insert Size	4956 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022188.1, NP_071524.1
RefSeq Size	4956 bp
RefSeq ORF	4956 bp
Locus ID	58946
UniProt ID	O55005
Gene Summary	putative axon guidance receptor [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.