Magi2 (NM_053621) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Acvrinp1; Acvrip1; S-SCAM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205298 representing NM_053621 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAAAAGCTTGAAAAAGAAAAGCCACTGGACTAGCAAAGTCCACGAGAGTGTCATTGGCAGAAACC CGGAGGGCCAGCTGGGCTTTGAACTGAAGGGGGGCGCCGAAAACGGACAGTTCCCCTACCTGGGGGAGGT GAAGCCGGGCAAGGTGGCCTATGAAAGCGGCAGCAAGTTGGTGTCCGAGGAGCTGCTGCTGGAGGTGAAC GAGACCCCCGTGGCGGGGCTCACCATCAGGGATGTTCTGGCCGTGATCAAACACTGCAAGGACCCCCTCC GGCTCAAGTGTGTCAAGCAAGGAGGAATTGTTGATAAGGATCTTCGTCACTACCTCAACTTAAGATTTCA GAAGGGTTCTGTTGACCACGAACTACAGCAAATCATCCGTGACAACCTCTACCTCCGCACAGTGCCATGC ACCACAAGGCCACATAAGGAGGGTGAGGTCCCTGGAGTGGACTACATTTTCATAACCGTTGAGGACTTTA TGGAATTGGAGAAAAGCGGTGCTCTCCTAGAAAGTGGGACCTATGAAGACAACTATTACGGCACTCCAAA GCCTCCAGCTGAACCAGCACCATTATTATTAAATGTAACAGATCAGATACTCCCGGGAGCTACTCCAAGT GCTGAGGGGAAGCGGAAAAGAAATAAGTCTGTGACCAACATGGAGAAAGCAAGTATAGAGCCACCGGAGG AAGAGGAGGAAGAAAGGCCTGTGGTCAACGGAAATGGCGTCGTCATAACACCAGAATCCAGTGAACATGA AGACAAAAGTGCAGGTGCCTCAGGGGAGACCCCCTCCCAGCCTTACCCTGCACCAGTGTACAGTCAGCCT GAAGAGCTCAAGGACCAGATGGATGATACAAAGTCAACAAAGCCCGAGGAGAATGAGGACTCCGACCCAT TGCCTGATAACTGGGAAATGGCCTACACAGAGAAGGGTGAAGTCTACTTCATTGACCATAACACAAAGAC AACATCATGGCTGGATCCACGACTTGCCAAAAAGGCTAAGCCTGCAGAGGAGTGCAAGGAAAATGAGCTT CCATATGGCTGGGAAAAAATCGATGATCCTATATATGGCACTTACTATGTCGACCACATAAATAGAAGAA CACAGTTTGAAAACCCTGTCCTGGAAGCAAAAAGGAAGCTACAGCAACATAACATGCCCCACACAGAACT TGGAACAAAGCCCCTGCAGGCCCCAGGTTTCCGAGAAAAGCCACTCTTCACCCGGGATGCATCCCAGTTG AAGGGAACATTCCTCAGCACCACCCTCAAAAAGAGCAACATGGGCTTTGGGTTTACGATCATCGGTGGAG ATGAGCCGGACGAGTTTCTACAGGTGAAGAGTGTGATCCCGGATGGGCCCGCCGCACAGGATGGGAAAAT GGAGACAGGTGATGTCATTGTCTATATTAATGAAGTTTGTGTCCTTGGACACACTCATGCAGATGTTGTC AAACTTTTCCAGTCTGTTCCTATTGGTCAGAGCGTCAACCTGGTGTTGTGTCGTGGCTACCCTTTGCCCT TTGATCCTGAAGACCCTGCCAACAGCATGGTGCCACCCCTTGCAATAATGGAGAGGCCACCTCCGGTGAT GGTCAATGGAAGACATAACTATGAAACATATTTGGAATACATTTCTCGGACCTCACAGTCGGTCCCAGAT ATTACAGACCGGCCACCTCATTCTTTGCACTCCATGCCAGCTGATGGCCAACTAGATGGCACATATCCAC CACCCGTCCACGATGACAATGTGTCTGTGGCTTCATCTGGAGCCACTCAAGCTGAACTTATGACCTTAAC CATTGTGAAAGGTGCCAAGGGATTTGGCTTTACTATTGCCGACAGTCCCACGGGACAGCGGGTGAAACAA ATACTTGACATTCAGGGATGCCCCGGGCTGTGTGAAGGAGACCTCATTGTTGAGATCAATCAACAGAATG TACAGAACCTGAGCCATACAGAAGTGGTTGATATACTTAAGGACTGCCCCGTCGGAAGCGAGACTTCTCT AATCATCCATCGAGGAGGTTTCTTTTCTCCATGGAAAACTCCAAAGCCTATGGTGGACCGGTGGGAGAAC CAAGGCAGCCCTCAAACAAGTTTATCTGCTCCGGCCGTCCCACAGAGCCTGCCCTTCCCACCTGCCCTTC ATAGGAGCTCCTTTCCTGACTCAACAGAGGCCTTTGACCCACGGAAGCCTGACCCGTATGAGCTCTACGA GAAATCGAGAGCCATTTATGAAAGTAGGCAACAAGTGCCACCCAGGACCAGTTTTCGAATGGATTCCTCT GGTCCAGATTATAAGGAATTGGATGTTCACCTTCGGAGGATGGAGTCTGGATTTGGCTTCAGAATCCTTG GGGGAGACGAGCCCGGACAGCCTATTTTGATCGGAGCTGTCATTGCGATGGGCTCAGCTGACAGAGACGG CCGTCTGCACCCAGGAGACGAGCTTGTCTACGTGGATGGGATCCCGGTGGCTGGCAAGACCCACCGGTAT GTCATCGACCTCATGCACCATGCGGCCCGCAACGGGCAGGTTAACCTCACTGTGAGAAGAAAGGTGCTAT GTGGAGGGGAGCCCTGCCCAGAGAATGGGAGGAGTCCAGGCTCTGTATCAACACACCACAGCTCTCCCCG CAGTGACTATGCCACCTACGCCAACAGCAACCACGCGGCCCCCAGTAACAATGCCTCACCCCCTGAAGGC TTTGCCTCGCACAGCCTGCAGACCAGTGACGTCATCATTCACCGAAAGGAAAACGAAGGCTTCGGCTTCG TCATCATCAGCTCCCTGAACAGGCCTGAGTCTGGAGCCACCATAACCGTGCCCCATAAAATCGGACGAAT CATCGATGGGAGCCCTGCAGATCGCTGCGCAAAACTCAAAGTGGGCGACCGGATCTTAGCAGTCAACGGC CAGTCTATCATCAACATGCCTCACGCTGACATCGTGAAGCTCATCAAGGATGCGGGTCTCAGCGTCACCC TCCGCATCATTCCTCAGGAGGAGCTCAACAACCCAACATCAGCACCCAGTTCAGAGAAGCAGAGCCCCAT GGCCCAGCAGCACAGCCCTCTGGCCCAGCAGCACAGCCCTCTGGCCCAGCCGAGCCCAGCCACCCCCAAC AGCCCAGTTGCACAGCCAGCTCCTCCTCAACCTCTCCAGCTGCAAGGACATGAAAATAGTTACAGGTCAG AAGTTAAAGCAAGGCAAGATGTGAAGCCAGACATCCGGCAGCCGCCCTTCACAGACTACAGGCAGCCGCC ACTGGACTACAGGCAACCCCCGGGAGGGGACTACTCGCAGCCCTCACCCTTGGACTACAGGCAGCACTCC CCAGACACCAGGCAGTACCCTCTGTCAGACTACAGGCAGCCACAGGATTTCGATTATTTCACTGTGGACA TGGAGAAAGGAGCCAAAGGATTTGGATTCAGCATCCGTGGAGGAAGGGAATACAAAATGGATCTGTACGT GTTGAGATTGGCGGAGGATGGGCCAGCCATAAGGAACGGCAGGATGAGGGTGGGAGATCAGATCATCGAA ATAAATGGGGAAAGCACACGAGACATGACCCACGCCAGAGCAATAGAACTCATCAAGTCGGGAGGAAGAA GAGTGCGGTTGCTGCTGAAACGAGGCACGGGGCAGGTCCCAGAGTATGGAATGGTGCCTTCCAGCCTCTC CATGTGCATGAAAAGTGACAAGCATGGGTCCCCATATTTCTACTTACTGGGCCACCCTAAAGACACGACG AACCCTACGCCTGGAGCGCTGCCGCTGCCGCCGCCCCAGGCCTGCCGGAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053621
Insert Size	3834 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053621.1, NP_446073.1
RefSeq Size	4767 bp
RefSeq ORF	3834 bp
Locus ID	113970
UniProt ID	O88382
Gene Summary	Seems to act as scaffold molecule at synaptic junctions by assembling neurotransmitter receptors and cell adhesion proteins. May play a role in regulating activin-mediated signaling in neuronal cells. Enhances the ability of PTEN to suppress AKT1 activation (By similarity). Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth.[UniProtKB/Swiss-Prot Function]

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