Mast2 (NM_001108005) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204960 representing NM_001108005 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCACGACAGCAGGGAGCAGGATATGGTTTCTGGACTTAGTCCCCTGCTCTTCAGAAAGCTTAGTA ATCCTGACATATTTTCACCTACTGGAAAAGCTAAACTCCAGCGACAACTTAGTCAGGATGACTGTAAGTT ACGGAGAGGAAGCCTGGCCAGTTCTCTGTCAGGTAAGCAGCTGCTCCCTTTGTCCAGCAGTGTACACAGC AGTGTGGGACAGGTAACTTGGCAGTCTACAGGAGAAGCATCAAACTTGGTTCGAATGAGAAACCAATCTC TTGGACAGTCTGCACCTTCCCTTACTGCTGGCTTGAAGGAATTGAGCCTTCCGAGGAGAGGCAGCTTCTG TCGGACGAGTAACCGCAAGAGCTTGATTGTAACCTCTAGCACATCACCCACACTACCAAGGCCACACTCG CCACTCCATGGCCACACGGGTAACAGTCCCTTGGACAGCCCTCGGAATTTCTCTCCAAATGCTCCTGCTC ACTTTTCCTTTGTTCCTGCCCGTAGGACTGATGGACGGCGCTGGTCTTTGGCCTCATTGCCTTCTTCAGG CTATGGAACCAACACTCCTAGCTCTACAGTCTCTTCATCGTGTTCCTCACAAGAAAAGCTTCATCAGTTA CCTTTCCAGCCGACAGCTGATGAACTACACTTTCTGACGAAGCATTTTAGCACAGAGAATGTACCAGATG AGGAGGGACGTCAGTCCCCAGCCATGCGGCCCCGTTCCCGCAGCCTCAGTCCTGGACGGTCCCCAGTTTC CTTTGACAGTGAAATAATAATGATGAATCATGTGTACAAAGAAAGATTCCCCAAGGCCACTGCACAAATG GAAGAGCGACTAGCTGAGTTCATTTCCTGTAACACCCCAGATAGTGTGCTGCCCTTGGCAGATGGAGCAC TAAGTTTTATTCATCATCAAGTAATTGAGATGGCCCGAGACTGCCTGGATAAATCTCGGAGTGGCCTCAT TACATCGCACTATTTCTATGAACTTCAAGAGAATTTGGAAAAGCTTCTACAAGATGCTCATGAACGCTCA GAAAGCTCAGACGTAGCCTTTGTGATTCAGCTGGTGAAAAAGCTGATGATCATCATTGCCCGCCCAGCTC GTCTCCTGGAATGCCTGGAGTTTGACCCTGAAGAATTTTACCACCTCTTAGAAGCAGCTGAGGGTCATGC CAAAGAGGGACATGGAATTAAATGTGACATTCCCCGCTACATCGTTAGCCAGCTGGGCCTCACACGGGAT CCCCTAGAGGAAATGGTCCAGTTGAGCAGCTATGACAGCCCTGATACTCCGGAGACAGACGATTCAGTTG AGGGGCGTGGGGCATCTCAGCCATCTAAAAAGACCCCCTCTGAAGAGGACTTTGAGACCATTAAGCTCAT CAGCAATGGCGCCTATGGGGCTGTCTTTCTGGTGCGGCACAAGTCCACTCGGCAGCGCTTTGCTATGAAG AAGATTAATAAGCAAAACCTAATCCTACGGAACCAGATCCAGCAGGCATTTGTGGAACGCGACATACTGA CTTTTGCTGAAAACCCCTTTGTAGTCAGCATGTTCTGCTCCTTTGAGACCAAGCGTCACTTATGCATGGT GATGGAATACGTTGAAGGGGGAGACTGTGCCACTCTGCTCAAGAACATCGGGGCCCTACCTGTGGACATG GTTCGTCTGTACTTCGCGGAAACGGTGCTGGCCTTGGAATACTTACACAACTACGGCATCGTACACCGTG ACCTCAAGCCTGACAACCTTCTAATTACATCCATGGGGCACATCAAACTCACTGACTTTGGACTTTCTAA AATTGGCCTCATGAGTTTGACAACCAACTTGTATGAGGGCCATATTGAAAAGGATGCCCGGGAGTTCCTA GACAAGCAGGTATGCGGGACCCCGGAATACATAGCACCTGAGGTGATCCTGCGTCAGGGATATGGGAAGC CAGTGGACTGGTGGGCCATGGGCATAATCCTGTATGAGTTCCTGGTGGGTTGCGTCCCTTTCTTTGGAGA CACTCCGGAGGAGCTCTTTGGGCAAGTGATCAGCGATGAGATTGTGTGGCCTGAGGGTGATGATGCACTT CCCCCAGATGCCCAAGACCTCACTTCCAAACTGCTTCACCAGAACCCACTAGAAAGACTGGGCACAAGTA GTGCCTATGAAGTGAAGCAGCACCCGTTCTTCATGGGTCTGGACTGGACAGGACTTCTTCGCCAGAAGGC TGAATTCATACCTCAACTGGAGTCAGAGGATGATACCAGCTACTTTGATACCCGTTCAGAACGATACCAC CATGTGGACTCCGAGGATGAGGAGGAAGTGAGTGAGGATGGCTGCCTTGAGATCCGCCAGTTTTCTTCCT GCTCTCCAAGGTTCAGCAAGGTTTACAGTAGCATGGAGAGGCTTTCCCTGCTTGAGGGACACCGGACACC ACCCCCAACCAAGCGCAGCCTGAGTGAGGAGAAGGAAGATCACTCAGATGGCTTGGCAGGACTGAAGGGC CGAGACCGCAGCTGGGTGATTGGGTCCCCTGAGATATTGCGGAAGCGGTTATCTGTGTCTGAGTCATCCC ACACAGAGAGTGATTCCAGCCCTCCAATGACAGTGCGGCACCGCTCAGGCCTCCCAGATGTGCCTCGTTG TGCTGAGGAGACCAGTAGCACCCCTCGGAAGCAACAACAGGAAGGTATTTGGGTCCTCACGCCCCCATCT GGAGAGGGGTCATCTAGGCCTGTTCCTGAACGACCACTGGAGAGGCAGCTGAAGCTGGACGAGGAGCCTC CTGGCCAAAGCAGTCGGTCTTGTCCAGCCATGGAGACACGAGGCCGTGGGACCCCACAATTAGCTGAGGA AGCTACAGCCAAAGCCATCAGTGACCTAGCTGTGCGCAGGGCCCGGCACCGGCTGCTCTCTGGGGACTCT ATAGAGAAGCGCACCACTCGCCCTGTCAACAAAGTGATTAAGTCAGCCTCAGCCACAGCCCTGTCCCTCC TCATTCCTTCAGAACACCATGCCTGTTCACCATTGGCCAGCCCCATGTCCCCACATTCTCAGTCATCCAA CCCATCATCCAGGGACTCTTCTCCAAGCAGGGACTTCTTGCCTGCCCTCGGCAGCCTGAGGCCTCCCATC ATCATCCACCGAGCTGGCAAGAAGTATGGCTTCACCCTGCGGGCCATTCGTGTCTACATGGGTGACTCAG ATGTCTACACTGTACACCATATGGTGTGGCATGTGGAGGACGGTGGTCCAGCCAGTGAAGCGGGGCTTCG TCAGGGTGACCTCATCACCCACGTCAATGGTGAGCCCGTGCACGGGCTGGTCCATACAGAAGTGGTAGAG CTGGTTCTGAAGAGTGGAAACAAGGTATCAATTTCAACAACTCCCTTGGAGAACACATCCATCAAAGTGG GGCCGGCTAGGAAAGGCAGCTCTAAGGCCAAGATGGCCCGAAGGAGCAAAAGGAGCCGGGGCAAGGACGG GCAGGAAAGCCGAAAAAGAAACTCCCTATTCCGGAAAATCACAAAGCAGGCCTCCTTGCTACACACCAGC CGCAGCCTTTCTTCCCTTAACCGCTCTTTGTCATCGGGGGAGAGTGGGCCCTGCTCTCCCACACACAGCC ACAGCCTCTCTCCCCGATCTCCTCCTCAGGGCTACCGGGTAGCCCCAGATACTGTGCACTCAGTAGGAGG GAATTCATCACAGAGCAGCTCTCCCAGCTCCAGTGTGCCCAGCTCTCCTGCTGGCTCCGGACATACACGG CCCAGCTCCCTTCATGGATTGGCACCCAAGCTCCAACGCCAGTACCGCTCGCCAAGGCGCAAGTCAGCAG GCAGCATCCCACTGTCACCATTGGCCCATACCCCTTCTCCACCAGCAGCAGCAGCTTCCCCTCAGCGTTC CCCATCACCCTTGTCTGGCCATGGGTCTCAGTCCTTTCCTACCAAGCTTCACTTGTCTCCTCCACTAGGT AGGCAGCTCTCACGGCCCAAGAGTGCAGAGCCACCCCGCTCTCCGCTACTTAAGAGGGTGCAGTCTGCTG AGAAGCTTGCGGCTGCACTGGCAGCTGCTGAGAAGAAGTTAGCGCCTTCCCGAAAGCATAGTCTTGACCT GCCCCACGGAGAGCTGAAGAAGGAACTGACACCCAGGGAAGCCAACCCTCTGGAGGTAGTTGGAACCAGA AGTGTGCTTTCTGGGAAAGGACCACTTCCAGGAAAGGGGGTGCTGCAGCCTGCTCCTTCACGGGCCCTTG GGACCCTACGGCAGGACCGAGCTGAACGCCGAGAGTCATTGCAAAAACAAGAAGCTATCCGGGAAGTAGA CTCCTCAGAAGATGACACTGATGAGGAGCCCGAGAACAGCCAGGCCACCCAGGAGCCAAGATTGTCCCCC CACCCAGAAGCAAGCCAGAATCTACTCCCTAAAGGTTCGGGAGAGGGTAGGGAAGAGGACACTTTCTTGC ATAGGGATCTAAAGAACCGGGGTCCTGTACTCTCGGGCCTGTTGACAGGGAATAGAATAGAAGTTCCTGG GCTCTCCCAGAGGAAGCTTTGGAGGCCACAAGCCTTTGAGGAAGCTACCAACTCCTTACAGGCCCCTAGC CTGAGCAGGTCTGGACCCACAAGCCCCACCCCCTCTGAAGGCTGCTGGGAGGCGCGGCAGCTCCACACAC AGGCACTAGCTACACTTCATCCCAGCTCTTCAGAACTCACCCCTACCAGTTGTTCTGCTGCCACCTCCAC CTCTGGAAAGCCAGGGACATGGTCCTGGAAATTCCTTATTGAGGGTCCAGACCGAGCATCCACAAACAAG AAGATAACAAGGACAGGTGAACCAGCTAACTCCCAAGATTTGGAAACCACAGTTCCAACTCAGGTTCCTA AGAACCTGTCTCCTGAGGAAGAGAAGCCACAGCCACCAAGTGTCCGTGGGCTGACCCATCCGCTTCTTGA GGTCCCCAGCCAGAACTGGTTATGGGAGTCTGGATGTGAGCAAATGGAGAAAGAGGACCCATCCCTGAGC ATCACCGAAGTGCCTGGTTCCTCAGGCAACAGGAGGCAGGACATTCCATGCAGAGCCCACCATCTGAGCC CAGAAACCCGGCCCAGTCTGCTCTGGAGAAGCCAAGAACTTGGGGGCCAGCAAGAGCATCAGGACTTAGC ACTGACATCAGATGAGCTTTTAAAGCAAACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108005
Insert Size	5214 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108005.1, NP_001101475.1
RefSeq Size	5399 bp
RefSeq ORF	5214 bp
Locus ID	313819

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