Cacna1f (NM_053701) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204450 representing NM_053701 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAATCTGAAGTCGGGAAAGACACCACCCCAGAACCCAGTCCAGCCAATGGGACCGGTCCTGGCC CTGAGTGGGGGCTCTGTCCTGGGCCTCCAACCGTGGGGACTGATACCAGTGGGGCATCGGGCCTGGGGAC CCCAAGACGGAGGACTCAGCACAACAAGCACAAGACCGTAGTGGTGGCCAGTGCGCAGAGATCACCTCGA GCACTCTTCTGCCTCACCTTAACCAATCCCATTCGTCGGTCCTGCATCAGCATTGTGGAGTGGAAGCCAT TTGATATTCTCATCCTCCTGACCATCTTTGCCAACTGTGTGGCATTGGGGGTATATATCCCCTTCCCTGA GGACGACTCCAACACTGCTACCCACAACTTGGAACAGGTAGAATATGTGTTCCTGGTGATTTTCACCGTG AAGACAGTGCTCAAGATCGTGGCCTATGGGCTGGTGCTCCATCCCAGCGCCTACATTCGCAATGGTTGGA ACCTACTCGACTTCATCATCGTTGTAGTCGGGCTGTTCAGCGTGTTGCTGGAGCAGGGACCTGGGCGGCC AGGAGATGCCCCGCATACTGGAGGAAAGCCGGGAGGCTTTGATGTAAAGGCACTGCGGGCATTTAGGGTG CTACGACCACTAAGGCTAGTGTCTGGGGTCCCGAGTCTGCACATAGTGCTCAATTCCATCATGAAGGCGC TTGTGCCGCTTCTGCACATTGCCCTGTTGGTGCTCTTCGTCATTATCATTTACGCCATCATCGGACTCGA GCTGTTCCTCGGACGAATGCACAAGACGTGCTACTTCCTGGGATCCGACATGGAGGCAGAGGAGGACCCG TCGCCTTGTGCATCTTCTGGCTCTGGCCGTTCATGCACACTGAACCAGACTGAGTGCCGTGGGCGCTGGC CAGGACCCAACGGTGGCATCACGAACTTTGACAATTTTTTCTTTGCCATGCTAACTGTGTTCCAGTGTAT TACCATGGAAGGCTGGACAGACGTCCTCTACTGGATGCAGGATGCCATGGGGTATGAGCTACCTTGGGTG TACTTTGTGAGCCTCGTCATCTTTGGGTCTTTCTTTGTCCTCAACCTTGTGCTTGGAGTCCTAAGTGGGG AGTTCTCCAAGGAAAGAGAAAAGGCAAAAGCCCGAGGTGACTTTCAGAAGCTTCGGGAGAAGCAGCAGAT GGAAGAGGACCTTCGGGGCTACCTGGACTGGATCACACAGGCTGAGGAGTTGGACCTTCATGACCCCTCA GTAGACGGCAACTTGGCTTCTCTTGCTGAAGAGGGACGGGCAGGCCATCGACCACAACTGTCAGAGCTGA CCAATAGGAGGCGCGGACGACTGCGTTGGTTCAGCCACTCCACTCGCTCCACACACTCCACCAGCAGTCA TGCCAGCCTTCCAGCCAGTGACACTGGTTCCATAACAGACACCCCCGGAGATGAGGATGAAGAGGAGGGG ACCATGGCCAGCTGTACACTCTGCCTAAACAAGATTATGAAAACCAAGGTCTGCCGCCACTTCCGCCGAG CCAACCGTGGTCTCCGTGCACGCTGCCGCCGGGCAGTCAAGTCCAATGCCTGCTACTGGGCTGTATTGCT GCTCGTCTTCCTCAACACGTTGACCATCGCTTCTGAGCACCATGGGCAGCCAGTGTGGCTCACCCAGACC CAAGAATATGCCAACAAGGTTCTGCTCTGCCTCTTCACCGTGGAGATGCTTCTCAAACTGTACGGGCTGG GCCCCTCTGTCTACGTTGCCTCTTTTTTCAACCGCTTTGACTGTTTCGTGGTCTGTGGGGGCATCCTAGA AACGACGTTGGTGGAGGTGGGGGCCATGCAGCCCCTCGGCATCTCAGTGCTCCGATGTGTGCGTCTCCTC AGGATCTTCAAGGTCACCAGGCACTGGGCATCCCTCAGCAATCTGGTGGCATCGCTGCTCAACTCCATGA AGTCCATCGCCTCCTTGCTGCTTCTCCTCTTTCTCTTTATCATCATCTTCTCCCTTCTTGGCATGCAGCT GTTTGGGGGCAAGTTCAACTTTGACCAGACCCACACTAAGAGAAGCACCTTTGATACCTTCCCCCAAGCC CTCCTCACCGTCTTTCAGATCCTGACTGGTGAGGATTGGAATGTAGTCATGTATGACGGTATCATGGCCT ACGGTGGTCCCTTCTTCCCAGGGATGCTGGTGTGTGTTTATTTCATCATCCTCTTCATCTGTGGCAACTA CATCCTACTGAACGTGTTTCTTGCCATTGCTGTGGATAACCTAGCCAGCGGGGATGCGGGTGCTGCCAAA GATAAGGGCAGAGAGAAGAGCAGTGAAGGAAACCCTCCCCAAGAGAACAAAGTATTGGTGCCTGGTGGAG AAAATGAGGACACAGAGGGCACAAAAAGTGAAGGAGCAGCACCAGGCATGGAGGAAGAGGAGGAAGAAGA GGAGGAGGAGGAGGAAGAAAATGGTGCAGGACATGTGGAACTCCTGCAGGAAGTTGTACCCAAGGAGAAG GTGGTACCCATCCCTGAAGGCAGTGCCTTCTTCTGCCTCAGCCAAACCAACCCGCTTCGCAAGGCCTGCC ACACTCTCATACACCATCACGTGTTCACCAGTCTCATCCTGGTGTTCATCATCCTCAGTAGTGTGTCCCT GGCTGCTGAGGACCCCATCAGAGCCCACTCTTTCCGCAACCATATTCTGGGGTACTTCGATTATGCCTTC ACTTCCATTTTCACCGTGGAGATTCTATTAAAGATGACGGTGTTTGGGGCTTTCCTGCACCAAGGTTCTT TCTGCCGTAGCTGGTTCAATCTGTTGGATCTGCTTGTGGTCAGTGTGTCCCTCATCTCCTTTGGCATCCA CTCCAGTGCCATCTCAGTTGTGAAGATTCTCCGAGTCCTCCGAGTCCTGCGGCCTCTCCGAGCCATTAAC CGAGCCAAGGGACTCAAGCATGTGGTGCAGTGTGTGTTTGTGGCCATCCGGACCATTGGAAACATCATGA TTGTCACCACCCTCTTGCAGTTCATGTTCGCCTGCATTGGTGTTCAGCTGTTCAAGGGAAAATTCTACAG TTGCACTGATGAAGCCAAACACACCCTGAAAGAATGCAAGGGCTCCTTCCTCATCTACCCTGATGGAGAT GTGTCACGACCTTTGGTCCGGGAACGCCTCTGGGTCAACAGTGATTTCAACTTTGACAACGTCCTTTCAG CCATGATGGCCCTGTTCACTGTCTCTACCTTTGAAGGCTGGCCTGCGCTACTATACAAAGCCATAGATGC ACACGCTGAAGACGAGGGCCCTATCTACAATTACCATGTGGAGATATCAGTATTCTTCATTGTCTACATC ATCATCATCGCCTTCTTCATGATGAACATCTTTGTGGGATTTGTCATCATCACATTCCGTGCCCAGGGAG AGCAGGAATATCAAAACTGTGAACTGGACAAGAACCAGCGCCAGTGTGTGGAATATGCACTCAAAGCTCA GCCACTCCGCCGATACATCCCCAAGAATCCACATCAGTACCGCGTGTGGGCCACAGTGAACTCAGCCGCC TTTGAGTACCTCATGTTTCTGCTCATCCTCCTCAACACAGTTGCCCTGGCCATGCAGCACTATGAGCAGA CGGCTCCCTTTAACTATGCCATGGACATACTCAACATGGTCTTCACTGGCCTCTTCACCGTTGAGATGGT GCTCAAAATCATTGCCTTTAAACCCAAGCATTACTTTGCCGATGCCTGGAATACATTCGATGCTCTTATT GTAGTGGGCAGCGTAGTCGACATTGCCGTCACAGAAGTCAATAATGGAGGCCATCTTGGTGAGAGCTCAG AGGACAGCTCCCGCATATCTATCACGTTCTTTCGCCTCTTCCGGGTCATGAGGCTGGTGAAGCTGCTCAG TAAGGGTGAGGGGATCCGCACACTGCTCTGGACATTCATCAAGTCTTTCCAGGCCTTGCCCCATGTGGCA CTTCTTATAGCAATGATATTCTTCATATATGCAGTCATTGGCATGCAGATGTTTGGCAAAGTGGCTCTTC AGGACGGCACACAGATAAATCGAAACAATAATTTCCAGACCTTTCCGCAGGCTGTGCTGCTTCTGTTCAG GTGTGCCACTGGTGAGGCATGGCAAGAGATAATGCTAGCCAGCCTTCCAGGAAATCGGTGTGACCCTGAG TCTGACTTTGGCCCAGGCGAGGAATTTACCTGTGGTAGCAATTTTGCCATTGTCTACTTTATCAGCTTCT TCATGCTCTGTGCCTTCCTGATTATAAATCTCTTTGTGGCTGTAATCATGGATAACTTTGATTACCTAAC CAGAGATTGGTCTATTCTGGGACCCCACCACCTCGATGAATTCAAGAGGATCTGGTCTGAATATGACCCC GGAGCCAAGGGCCGCATCAAGCACTTGGATGTGGTTGCCCTGCTGAGACGCATCCAGCCTCCACTGGGAT TTGGAAAGCTGTGCCCACACCGAGTGGCCTGCAAGAGACTGGTGGCCATGAATGTTCCCCTCAACTCAGA CGGAACAGTGACATTCAACGCGACACTCTTTGCCCTGGTGCGGACATCCCTGAGGATCAAGACAGAAGGG AACCTGGATCAAGCCAACCAGGAGCTTCGGATGGTCATCAAAAAGATCTGGAAGCGGATAAAGCAGAAAT TGTTAGATGAGGTCATCCCCCCTCCAGATGAGGAGGAGGTCACTGTGGGAAAATTCTATGCCACATTTCT CATCCAAGATTATTTCCGAAAATTCCGGAGAAGGAAAGAAAAGGGGCTACTAGGAGCAGATGCCCCAACA AGCACATCCTCTGTCCTCCAGGCTGGTCTAAGGAGCCTACAGGACTTGGGTCCTGAGATCCGACAGGCCC TCACCTATGCCCTTGAGGAAGAAGAAGAAGAAGAAGAGGCCCGCCAGGTCAAGGAAGCTGAGGAGGAGGA AGCTGAGACCACCCCAGAAACATACAAAGACTCCATAGACTCCCAGCCCCAAGCTCAATGGAACTCGAGG ATTTCGGTGTCTCTACCCAAGTTAAGAGAAACTCCAGATTCTCTTTCAACTGGGCCTAGTGATGATGATG GAGTGGCTCCCAACTCCAGGCAACCCAGTGGGCTACAGGCTGGATCCCAACCCCACAGGAGAGGCTCTGG GGTTTTCATGTTCACTATCCCAGAAGAAGGAAGTACTCAGCTCAAGGGAGTTCAAGGGCAAGACAATCAG AATGAGGAACAGGAAGTCCCTGACTGGACCCCCAACCTGGATGAGCAGGCAGGGATGCCCTCCAACCCAG TCCTTTTACCACCTCACTGGTCCCAGCAACATGTAAATGGGCACCATGTACCACGCCGACGTTTGCTACC CCCCACGCCTGCAGGTCGGAAGCCCTCCTTCACCATCCAGTGTCTGCAACGCCAGGGCAGTTGTGAGGAT TTGCCCATCCCAGGCACCTACCATCGTGGACGGACCTCAGGACCAGGCAGGGCTCAGGGTTCCTGGGCAG CCCCTCCTCAGAAGGGTCGACTGCTCTATGCCCCCCTGTTGCTGGTGGAGGAATCTACAGTGGGTGAAGG ATACCTTGGCAAGCTCGGCGGCCCACTGCGTACCTTCACTTGTCTGCAAGTGCCTGGAGCTCCTTCAGAT CCCAGTCACCGCAAGAGGGGCAGTGCTGACAGTTTGGTGGAGGCTGTGCTTATCTCCGAAGGCCTAGGCC TCTTTGCCCAAGACCCACGATTTGTGGCCCTGGCCAAGCAAGAGATTGCAGATGCATGTCACCTGACCCT GGATGAGATGGACAGTGCTGCCAGTGACCTGCTGGCACAGAGAACCACCTCCCTCTACAGTGACGAGGAG TCTATTCTCTCCCGCTTTGATGAAGAGGACCTGGGAGACGAGATGGCCTGCGTCCATGCCCTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053701
Insert Size	5946 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053701.1, NP_446153.1
RefSeq Size	5975 bp
RefSeq ORF	5946 bp
Locus ID	114493
Gene Summary	mutation of the human homolog causes incomplete X-linked congentital stationary night blindness (CSNB2) [RGD, Feb 2006]

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