Grin1 (NM_017010) Rat Untagged Clone
CAT#: RN204265
Grin1 (untagged ORF) - Rat glutamate receptor, ionotropic, N-methyl D-aspartate 1 (Grin1), (10 ug)
CNY 10,312.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | GluN1; NMDAR1; NR1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN204265 representing NM_017010
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACCATGCACCTGCTGACATTCGCCCTGCTTTTTTCCTGCTCCTTCGCCCGCGCCGCCTGCGACC CCAAGATCGTCAACATCGGCGCGGTGCTGAGCACGCGCAAGCATGAACAGATGTTCCGCGAGGCAGTAAA CCAGGCCAATAAGCGACACGGCTCTTGGAAGATACAGCTCAACGCCACTTCTGTCACCCACAAGCCCAAC GCCATACAGATGGCCCTGTCAGTGTGTGAGGACCTCATCTCTAGCCAGGTCTACGCTATCCTAGTTAGCC ACCCGCCTACTCCCAACGACCACTTCACTCCCACCCCTGTCTCCTACACAGCTGGCTTCTACAGAATCCC TGTCCTGGGACTGACTACCCGAATGTCCATCTACTCTGACAAGAGTATCCACCTGAGTTTCCTTCGCACG GTGCCGCCCTACTCCCACCAGTCCAGCGTCTGGTTTGAGATGATGCGAGTCTACAACTGGAACCACATCA TCCTGCTGGTCAGCGACGACCACGAGGGACGGGCAGCGCAGAAGCGCTTGGAGACGTTGCTGGAGGAACG GGAGTCCAAGGCAGAGAAGGTGCTGCAGTTTGACCCAGGAACCAAGAATGTGACGGCTCTGCTGATGGAG GCCCGGGAACTGGAGGCCCGGGTCATCATCCTTTCTGCAAGCGAGGACGACGCTGCCACAGTGTACCGCG CAGCCGCAATGCTGAACATGACGGGCTCTGGGTACGTGTGGCTGGTCGGGGAACGCGAGATCTCTGGGAA CGCCCTGCGCTACGCTCCTGATGGCATCATCGGACTTCAGCTCATCAATGGCAAGAATGAGTCAGCCCAC ATCAGTGACGCCGTGGGCGTGGTGGCACAGGCAGTTCACGAACTCCTAGAGAAGGAGAATATCACTGACC CACCGCGGGGTTGCGTGGGCAACACCAACATCTGGAAGACAGGACCATTGTTCAAGAGGGTGCTGATGTC TTCTAAGTATGCGGACGGAGTGACTGGCCGTGTGGAATTCAATGAGGATGGGGACCGGAAGTTTGCCAAC TATAGTATCATGAACCTGCAGAACCGCAAGCTGGTGCAAGTGGGCATCTACAATGGTACCCATGTCATCC CAAATGACAGGAAGATCATCTGGCCAGGAGGAGAGACAGAGAAACCTCGAGGATACCAGATGTCCACCAG ACTAAAGATAGTGACAATCCACCAAGAGCCCTTCGTGTACGTCAAGCCCACAATGAGTGATGGGACATGC AAAGAGGAGTTCACAGTCAATGGTGACCCAGTGAAGAAGGTGATCTGTACGGGGCCTAATGACACGTCCC CAGGCAGCCCACGCCACACAGTGCCCCAGTGCTGCTATGGCTTCTGCATAGACCTGCTCATCAAGCTGGC GCGGACCATGAATTTTACCTATGAGGTGCACCTGGTGGCAGATGGCAAGTTTGGCACACAGGAGCGGGTA AACAACAGCAACAAAAAGGAGTGGAACGGAATGATGGGCGAGCTACTCAGTGGCCAAGCGGACATGATTG TGGCACCACTGACCATCAACAATGAGCGTGCGCAGTACATAGAGTTCTCCAAGCCCTTCAAGTACCAGGG CCTGACCATTTTGGTCAAGAAGGAGATTCCCAGGAGCACACTGGACTCATTTATGCAGCCTTTTCAGAGC ACACTGTGGTTGCTAGTAGGACTGTCAGTTCATGTGGTGGCTGTGATGCTGTACCTGCTGGACCGCTTCA GTCCCTTTGGCCGATTCAAGGTGAACAGTGAGGAGGAGGAGGAAGATGCACTGACCCTGTCCTCTGCCAT GTGGTTTTCCTGGGGCGTCCTGCTCAACTCCGGCATTGGGGAAGGTGCCCCCCGGAGTTTCTCTGCACGT ATCCTAGGCATGGTGTGGGCTGGTTTCGCCATGATCATAGTGGCTTCCTACACTGCCAACTTGGCAGCTT TCCTGGTGCTGGATCGGCCTGAGGAGCGCATCACGGGCATCAATGACCCCAGGCTCAGAAACCCCTCAGA CAAGTTCATCTACGCAACTGTAAAGCAGAGCTCCGTGGACATCTACTTCCGGAGGCAGGTGGAGTTGAGT ACCATGTACCGGCACATGGAAAAACACAATTACGAGAGCGCAGCTGAGGCCATCCAGGCTGTGCGGGACA ACAAGCTGCACGCCTTTATCTGGGACTCGGCCGTGCTGGAGTTTGAGGCTTCACAGAAGTGCGATCTGGT GACCACGGGTGAGCTGTTCTTCCGCTCAGGCTTTGGCATCGGCATGCGCAAGGACAGCCCCTGGAAGCAG AACGTTTCCCTGTCCATACTCAAGTCCCATGAGAATGGCTTCATGGAAGATCTGGATAAGACATGGGTTC GGTATCAGGAATGCGACTCCCGCAGCAATGCTCCTGCAACCCTCACTTTTGAGAACATGGCAGGGGTCTT CATGCTGGTGGCTGGAGGCATCGTAGCTGGGATTTTCCTCATTTTCATTGAGATCGCCTACAAGCGACAC AAGGATGCCCGTAGGAAGCAGATGCAGCTGGCTTTTGCAGCCGTGAACGTGTGGAGGAAGAACCTGCAGG ATAGAAAGAGTGGTAGAGCAGAGCCCGACCCTAAAAAGAAAGCCACATTTAGGGCTATCACCTCCACCCT GGCCTCCAGCTTCAAGAGACGTAGGTCCTCCAAAGACACGAGCACCGGGGGTGGACGCGGCGCTTTGCAA AACCAAAAAGACACAGTGCTGCCGCGACGCGCTATTGAGAGGGAGGAGGGCCAGCTGCAGCTGTGTTCCC GTCATAGGGAGAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_017010 |
Insert Size | 2817 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_017010.1, NP_058706.1 |
RefSeq Size | 4280 bp |
RefSeq ORF | 2817 bp |
Locus ID | 24408 |
UniProt ID | P35439 |
Gene Summary | subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (1a) has the same N- and C-termini but is shorter compared to isoform 1b. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR204265 | Grin1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, N-methyl D-aspartate 1 (Grin1), (10 ug) |
CNY 10,992.00 |
|
RR204265L3 | Lenti ORF clone of Grin1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, N-methyl D-aspartate 1 (Grin1), (10 ug) |
CNY 13,780.00 |
|
RR204265L4 | Lenti ORF clone of Grin1 (mGFP-tagged ORF) - Rat glutamate receptor, ionotropic, N-methyl D-aspartate 1 (Grin1), (10 ug) |
CNY 15,200.00 |