Man2b1 (NM_199404) Rat Untagged Clone

CAT#: RN204232

Man2b1 (untagged ORF) - Rat mannosidase, alpha, class 2B, member 1 (Man2b1), (10 ug)



  "NM_199404" in other vectors (3)

CNY 16,250.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Manb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN204232 representing NM_199404
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCACCGGATCGCTTGCCCCTGGTGTCCGTGCTGGTGGCGGGAACACTGGGTGGCTCTGGATGAGCT
CCTGCAACTTAGGTTTGCCAGTCCTGTCTATTTCTTTTCTTATTTGGTTGTTACTGGCTGCGCCTGGCGC
TCAGGCCGCGGGTTACAAGACATGTCCCACCACGAAGCCAGGCATGCTCAACGTGCACCTGCTGCCTCAC
ACACACGATGATGTGGGCTGGCTGAAGACAGTAGACCAATACTACTACGGCATCATGAGTGACGTCCAGC
ATGCATCTGTTCAGTACATCCTAGACTCAGTGATTTACTCTCTACTGAACGATCCCACCCGTCGCTTCAT
ATACGTGGAAATGGCTTTCTTCTCCCGTTGGTGGAAGCAGCAGACCAATGTAACACAGGACGCTGTACGG
AACCTGGTGCGCCAGGGGCGCCTGGAGTTTGTCAATGGTGGCTGGGTAATGAATGATGAGGCAGCCACTC
ACTATGGTGCTATTGTGGACCAGATGACACTTGGGCTACGCTTCCTGCAGGACACATTTGGTAGTGATGG
ACTTCCCCGTGTGGCCTGGCACATTGACCCTTTTGGCCATTCTCGAGAACAGGCCTCCCTGTTTGCCCAG
ATGGGTTTTGATGGCTTCTTCCTCGGGCGCATTGACTACCAAGATAAATTTAACCGGAAGAGAAAGCTGA
AGATGGAGGAACTCTGGAGGGCTAGCGCCAGCTTGAAGCCCCCTGCTGCTGACCTCTTTACTGGTGTGCT
CCCCAATAATTACAACCCTCCAAAAGACCTGTGCTGGGATGTGCTGTGTACAGACCCTCCCGTGGTGGAC
GATCCCACAAGCCCTGAGTTTAATGCCAATAAGTTGGTTGATTACTTCCTGAACCTCGCCTCTTCCCAGA
AAAAGTATTACCGAACCAACCACACCGTGATGACCATGGGCTCAGACTTCCAGTATGAGAATGCCAACAT
GTGGTTCAAAAACATGGACAAGCTTATCCGCCTGGTCAACGAGCAGCAGGCAAACGGGAGCAAGGTCCAC
GTGCTCTACTCCACCCCTTCGTGTTACCTCTGGGAGCTGAACAAGGCCAATCTCACTTGGACAGTGAAGG
AGGACGACTTCTTTCCTTATGCTGATGGCCCCCACATGTTCTGGACTGGCTATTTCTCCAGTAGGCCAGC
CCTCAAGCGCTATGAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAACCAGCTGGAAGCACTGGTGGGT
CCCGAGGCCAAAGTGGGACCCTACGGCTCAGGAGACAGTGCACCCCTCAATGAGGCAATGGCGGTGCTGC
AGCACCACGATGCTGTCACTGGTACTGCAAGGCAGAATGTTGTCAATGACTATGCGAAGCAGCTGGCGGC
AGGCTGGGGACCCTGTGAGGTTCTCGTGAGCAACGCTCTGGCGCGACTCAGCCTTTACAAACAGAACTTC
TCATTCTGTCGGGAGATCAACATCAGTATCTGCCCAGCCAGCCAGACGTCAGAGCACTTCCGGGTAATTA
TTTACAACCCCGTGGGGCGGAAGGTGGATCTGATGGTTCGGCTGCCGGTCAGTGAAGGCATCTTCCTTGT
GAAGGACCCTAACGATAGGAGAATTTCCAGTAATGTGGTGATGGTTCCCAGTGCTTACAGTAAGACGTAT
CAATGGGAGCTGCTGTTCCCAGCCTCAGTGCCTGCCCTGGGCTTCAGCATCTACTCTGTGAACAAAATGA
GTGGCCATAACCATCAGGCCCACAACCTAACGGCCAGGCCCAAGAAATCCAAGTCCCGTGTCTTAGTCAT
TGAAAATAAGTACATACGGGCCACATTTGACTCTGACACAGGGTTATTGAGGAAGATTGAAAACCTGGAA
CAGAACATTTCGCTCCCTGTGAGACAAGGCTTCTTTTGGTACAATGCCAGCGCTGGTGATGAGGAGAGTC
CTCAGGCCTCTGGTGCCTACATCTTCAGACCCAGTCATCGTAAACCACTGCCTGTGAGCCACTGGGCTCA
GGTCACTTTGGTGAAGACAAACTTGGTGCAGGAGGTACACCAGAATTTTTCAGCCTGGTGTTCCCAAGTG
ATTCGCCTGTATGAAGGACAGCGTCATTTGGAGCTGGAGTGGACGGTGGGACCAATCCCAGTGAAAGATG
ACTGGGGAAAGGAAGTCATCAGCCGCTTTAACACCCCGATGAGAACCAGGGGGCAGTTCTTCACGGACAG
CAACGGCCGGGAAATCCTGAAGAGGAGGGACGATTTCCGACCCACCTGGACATTAAATCAGACTGAACCA
GTGGCTGGAAATTACTACCCTGTCAACACTCGCATTTACATCACGGACGGGCATATGCAGCTGACCGTGC
TGACCGACCGTTCCCAGGGGGGCAGCAGTCTGCTGGATGGCTCATTGGAGCTCATGGTTCACCGACGGCT
GTTGGTAGATGATGAACGTGGGGTGGCGGAACCCTTGCTGGAGACAGATACGGGGGATAAGGTTCGAGGG
CGCCACCTTGTGATTTTGAGTTCTGTCAGTGATGCGGCTGCAAGACACCGACTGCTGGCGGAGCAGGAAG
TCCTGGCCCCTCAGGTGGTGCTGGCCCACGGGGGTAGCAGTCCCTATCACTCGCAGGCACCAAAAATGCA
GTTCTCCGCACTTCGCCGGGAGCTACCTCCGCAGGTGCATCTGCTCACCCTGGCTCGCTGGGGTCCAAAG
ATGCTGCTGCTGCGCTTGGAGCACCAGTTCGCTGTGAAGGAAGATTCAAATCGCAACCTGAGCTCTCCGG
TGACCTTGAACTTGCAGAACCTGTTCAAGACCTTCACTATCAACTACCTGCAGGAGACCACACTGGCAGC
CAACCAGCCCCTATCCAGGGTTTCCAGGCTCAAGTGGATGACAGATACTGGTCCCATCTCCTACCCTGCT
CCTTCCCGGTTGGACCCTACCTCAATCACACTGCAGCCTATGCAAATCCGTACCTTCCTGGCCTCGGTTC
AATGGCAGAGACTCATCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_199404
Insert Size 3030 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_199404.1, NP_955436.1
RefSeq Size 3150 bp
RefSeq ORF 3030 bp
Locus ID 361378
Gene Summary mouse homolog is a lysosomal alpha-mannosidase which cleaves alpha-linked mannose residues on glycoproteins [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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