Atn1 (NM_017228) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Drpla
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204131 representing NM_017228 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACACGACAGAATAAAGACTCGATGTCAATGAGGAGTGGACGGAAGAAAGAGGCCCCCGGGCCCC GGGAAGAGCTGAGATCGAGGGGCCGGGCCTCCCCTGGAGGGGTCAGCACGTCCAGCAGTGATGGCAAAGC CGAGAAATCCAGGCAGACAGCCAAGAAGGCCCGAGTAGAGGAGACCTCTACCCCAAAAGCCAACAAGCAG GGCCGGAGCGAGGAGATCTCAGAGAGTGAAAGCGAAGAGACCAGTGCGCCCAAAAAGACCAAGACCGAGG AGCTTCCGCGCCCACAGTCTCCCTCGGATCTGGACAGCTTAGATGGCCGCAGCATCAACGATGACGGCAG CAGTGACCCTAGAGATATAGACCAGGACAACCGAAGCACATCCCCCAGCATCTACAGCCCTGGCAGTGTG GAAAATGACTCGGATTCATCCTCTGGCCTGTCCCAGGGCCCGGCTCGCCCCTACCACCCACCTCCACTCT TTCCTCCTTCCCCTCCACCACCAGACAGCATTCCCCGACAGCCAGAGTCTGGCTTTGAACCGCACCCTTC TGTGCCGCCTACTGGATATCATGCTCCAATGGAGCCCCCCACATCTCGGTTATTCCAGGGCCCACCTCCT GGAGCTCCTCCCCCACACCCACAGCTCTACCCTGGGAGTGCTGGTGGAGGTGTTTTATCTGGACCCCCCA TGGGTCCCAAAGGGGGAGCAGCCGCCTCCTCAGTGGGTCCCCCTAGTGGGGGCAAGCAGCACCCCCCACC CACTACCCCAATTCCAATATCAAGCTCTGGGGCCAGTGGCGCTCCTCCAGCAAAGCCCCCCAATACTCCA GTGGGTGCTGGGAACTTACCTTCTGCTCCACCACCAGCTACTTTCCCCCACGTGACACCAAACCTGCCTC CTCCACCTGCCCTGAGACCCCTCAACAATGCCTCAGCCTCTCCTCCTGGCATGGGGGCTCAGCCAATCCC TGGGCATCTGCCCTCTCCCCATGCCATGGGGCAGGGTATGAGTGGACTTCCTCCTGGCCCAGAGAAGGGC CCAACCCTGGCCCCCTCTCCCCACCCTTTACCCCCGGCTTCTTCCTCCGCTCCTGGGCCCCCAATGCGGT ACCCGTATTCATCCTGCTCCAGTAGCTCTGTTGCAGCGTCTTCTAGTTCCTCCGCCGCCACCTCCCAGTA CCCAGCTTCCCAGACCCTGCCCAGTTACCCTCACTCCTTCCCCCCACCAACAAGTATGTCTGTCTCTAAT CAGCCACCCAAGTACACCCAGCCTTCTCTCCCATCCCAGGCTGTGTGGAGCCAGGGCCCACCTCCTCCTC CTCCTTATGGCCGCCTCTTGCCCAACAACAACACTCATCCGGGCCCTTTCCCTCCTACCGGGGGTCAATC CACAGCCCACCCGCCAGCCCCTGCACATCACCATCACCAGCAGCAGCAGCAGCCACAGCCACAGCCACAG CCACAGCCGCAACAACATCATCATGGAAACTCTGGGCCCCCTCCACCTGGGGCATATCCTCACCCCCTAG AGAGCAGTAACTCCCACCATGCACACCCTTACAACATGTCACCCTCCCTGGGGTCTTTGAGGCCCTACCC ACCAGGGCCAGCACACCTGCCTCCGTCTCATGGCCAGGTGTCTTATAGCCAAGCAGGTCCCAATGGTCCC CCGGTTTCTTCTTCTTCCAACTCTTCCGGGTCTTCGTCTCAAGCCGCCTATTCATGCTCACACCCTTCTT CATCCCAGGGCCCCCAAGGGGCATCCTACCCCTTCCCACCAGTTCCTCCAATCACCACCTCCTCAGCTAC TCTTTCCACTGTCATCGCCACCGTGGCTTCCTCGCCAGCAGGTTATAAGACAGCCTCACCACCTGGCCCC CCTCAGTACAGCAAGAGAGCCCCATCCCCAGGGTCCTACAAGACAGCCACCCCGCCTGGATACAAGCCAG GGTCACCACCGTCCTTCAGAACAGGGACCCCACCTGGCTATCGAGGCACCTCTCCGCCAGCAGGCCCAGG GACCTTCAAGCCAGGTTCACCCACCGTGGGGCCGGGGCCCCTACCACCCGCGGGGCCTTCAAGTTTGTCA TCTCTGCCTCCACCACCCGCGGCCCCGACTACAGGGCCGCCCCTGACTGCCACGCAGATCAAACAGGAAC CAGCTGAAGAGTATGAAACCCCCGAGAGTCCGGTGCCTCCGGCTCGCAGCCCCTCGCCCCCTCCTAAGGT GGTGGACGTGCCTAGCCATGCCAGCCAGTCAGCCAGGTTCAATAAGCACCTGGACCGTGGCTTCAACTCG TGCGCGCGCAGCGACCTGTACTTTGTGCCGCTGGAGGGATCCAAGCTGGCCAAGAAGCGCGCGGACCTGG TGGAGAAAGTGCGGCGCGAGGCCGAGCAGCGCGCGCGCGAAGAAAAAGAGCGCGAGCGCGAGCGGGAACG TGAGAAGGAGCGCGAGCGCGAGAAAGAGCGCGAGCTGGAGCGCAGTGTGAAATTGGCCCAGGAGGGCCGT GCTCCAGTGGAGTGCCCGTCCCTGGGTCCAGTGCCCCATCGGCCTCCCTTTGAACCTGGCAGCGCTGTGG CTACCGTGCCCCCTTACCTGGGTCCTGATACTCCAGCCCTGCGCACTCTCAGTGAATACGCCCGACCTCA TGTCATGTCTCCTGGCAATCGCAACCACCCATTCTATGTGCCCTTGGGGGCAGTGGACCCGGGGCTTCTG GGTTACAATGTCCCAGCCCTGTACAGCAGCGACCCAGCTGCCCGAGAACGGGAGCGGGAAGCCCGTGAAC GTGACCTCCGTGACCGGCTCAAGCCTGGCTTTGAGGTGAAACCTAGTGAGCTGGAACCCCTACATGGGGT CCCCGGGCCAGGCCTGGATCCCTTCCCCCGACACGGGGGCCTGGCTCTACAACCTGGGCCACCTGGCCTG CATCCTTTCCCTTTTCATCCGAGCCTGGGGCCCCTGGAACGAGAACGGCTAGCGCTGGCAGCTGGGCCAG CCCTGCGTCCTGACATGTCTTATGCTGAGCGGTTGGCAGCTGAAAGGCAGCATGCAGAAAGGGTGGCAGC CCTGGGCAATGACCCACTAGCCCGGCTGCAGATGCTCAACGTGACTCCCCATCATCACCAGCACTCCCAC ATCCACTCTCACCTACACCTGCACCAGCAGGATGCCATCCATGCGGCCTCCGCCTCGGTGCACCCTCTCA TTGACCCCCTGGCCTCAGGGTCTCACCTTACCCGGATCCCCTACCCAGCTGGGACCCTCCCTAATCCCCT TCTTCCTCACCCTCTGCACGAGAACGAAGTTCTTCGTCACCAGCTCTTTGCTGCCCCTTACCGGGACCTA CCGGCCTCTCTATCTGCGCCAATGTCAGCCGCCCATCAGCTGCAGGCCATGCACGCACAGTCAGCCGAGC TGCAGCGCTTGGCACTGGAACAGCAACAGTGGCTGCACGCTCATCACCCATTGCACAGCGTGCCGCTGCC TGCCCAGGAGGACTACTACAGTCACCTGAAGAAGGAGAGTGACAAGCCGCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017228
Insert Size	3555 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017228.3, NP_058924.2
RefSeq Size	4338 bp
RefSeq ORF	3555 bp
Locus ID	29515
Gene Summary	widely expressed gene; expansion of CAG trinucleotide repeat in human homolog causes dentatorubral pallidoluysian atrophy, a degenerative neurological disorder similar to Huntington disease [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.