Myh7b (NM_001107794) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204114 representing NM_001107794 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGATATGAATGAACTTGGAGAATCAGCCTGCTACCTCCGCCAGGGATACCAGGAAATGATGAAGG TGCACACTGTCCCATGGGATGGCAAGAAACGGGTCTGGGTGCCTGATGAACAGGATGCCTATGTCGAAGC TGAGGTCAAGACTGAAGCTACTGGAGGCCGAGTCACTGTGGAGACCAAAGACCAGAAGGTGCTGACAGTG CGTGAAACGGAAATGCAGCCCATGAACCCACCCCGCTTCGACTTACTGGAGGACATGGCCATGATGACGC ACCTCAACGAGGCTGCAGTGCTGCACAACCTGCGCCAGCGTTATGCTCGCTGGATGATCTACACATACTC GGGCCTCTTCTGTGTCACCATTAACCCATACAAATGGCTCCCGGTCTACACAGCTACTGTGGTGGCCGCC TACAAGGGCAAGCGCCGCTCGGAGGCCCCGCCTCATATATATGCAGTGGCAGATAATGCCTACAATGACA TGCTCCGCAACAGAGAGAACCAGTCCATGCTGATCACCGGAGAGTCGGGGGCCGGTAAGACGGTTAACAC CAAGCGGGTCATTCAGTATTTTGCCATCGTCGCTGCCCTGGGAGACGGGCCGGGCAAGAAGGCACAATTT CTGGCAACAAAAACCGGGGGCACCCTTGAGGATCAAATCATCGAGGCTAACCCTGCCATGGAGGCTTTTG GTAACGCCAAGACTCTGAGGAACGACAACTCCTCCCGTTTTGGCAAGTTCATCCGCATCCACTTTGGTCC CACTGGGAAGCTGGCCTCTGCGGACATTGACAGCTATCTCCTGGAAAAGTCTCGAGTGATCTTCCAGCTG CCCGGTGAGCGTGGTTACCATGTCTACTACCAAATCCTCTCCGGGAAGAAGCCAGAGCTGCAGGACATGC TGCTCCTCTCCATGAACCCCTACGACTACCACTTCTGCAGTCAGGGGGTCACCACCGTGGATAACATGAA CGACGGGGAGGAGCTTATCGCCACTGATCATGCAATGGACATCCTGGGCTTCAGCGTGGATGAGAAGTGC GCATGCTATAAGATCGTGGGCGCCCTCCTGCACTTTGGCAACATGAAGTTCAAACAGAAGCAGAGAGAGG AACAGGCTGAGGCAGACGGCACTGAGAGTGCCGACAAGGCTGCCTACCTGATGGGAGTCAGCAGTGGAGA CCTCCTCAAAGGTCTTCTGCACCCCCGGGTTCGTGTGGGAAACGAGTATGTGACCAAGGGCCAGAGTGTG GAGCAGGTGGTATTTGCTGTGGGGGCCCTGGCCAAGGCTACCTATGACCGGTTGTTCCGATGGCTGGTAT CTCGAATCAATCAGACCCTGGACACAAAGCTCCCCCGTCAGTTCTTCATTGGGGTCCTGGATATCGCTGG TTTTGAGATCTTTGAGTTTAACAGCTTCGAGCAACTGTGTATCAACTTCACCAACGAGAAACTGCAGCAG TTCTTCAACCAGCACATGTTCGTGCTGGAGCAGGAGGAGTACAAACGGGAGGGCATTGACTGGGTCTTCA TCGACTTCGGCCTTGACCTGCAGCCCTGCATCGACCTCATCGAGAAGCCTCTGGGCATTCTGTCCATCCT GGAGGAGGAGTGCATGTTCCCCAAGGCTTCAGACGCAAGCTTCAGGGCCAAGCTCTATGACAATCACTCT GGGAAGTCACCTAATTTCCAGCAGCCTCGCCCAGACAAGAAACGCAAGTACCAGGCTCACTTCGAGGTGG TCCACTACGCAGGCGTGGTACCTTATAGCATTGTGGGCTGGCTGGAGAAAAACAAGGACCCCCTCAATGA GACCGTGGTCCCCATCTTCCAAAAGTCACAGAATAGGCTCTTGGCCACCCTCTATGAGAACTATGCAGGT TCTTGCTCCACCGAGCCCCCCAAGTCTGGGGTGAAAGAGAAACGAAAGAAAGCAGCATCATTTCAGACAG TGTCTCAGCTTCACAAGGAGAACCTCAACAAGCTTATGACCAACCTGCGGGCCACACAGCCCCACTTCGT CCGTTGTATCGTCCCCAACGAGAACAAGACTCCAGGGGTCATGGATTCCTTCTTGGTGCTACACCAGCTA CGCTGTAACGGGGTCCTAGAGGGGATCCGGATCTGCCGCCAAGGGTTCCCCAACAGACTGCTGTATGCTG ACTTCAGGCAGCGGTACCGAATCCTGAACCCCAGTGCCATCCCAGATGACACCTTTGTGGACAGCAGGAA GGCCACAGAGAAGCTGCTGGGCTCTCTGGACATTGACCACACCCAGTATCAGTTTGGCCACACCAAGGTG TTCTTCAAGGCTGGGCTTCTAGGCATCCTGGAGGAGCTCCGGGACCAGCGTCTGGCTAAGGTGCTGACAC TGCTGCAGGCTCGGAGCCGGGGCCGCCTCATGCGTCTGGAGTACCAGCGCATGCTAGGAGGAAGGGATGC CCTGTTCACCATCCAGTGGAACATCCGGGCCTTCAATGCCGTCAAGAACTGGTCCTGGATGAAGCTCTTC TTCAAGATGAAGCCACTGCTGCGCTCAGCACAGGCAGAGGAAGAGCTGGCAGCACTTCGGGCAGAACTGC GGGGGCTGCGGGGGGCCCTGGCTACGGCTGAGGCCAAGCGTCAGGAGCTGGAGGAGACTCAAGTCAGTGT CACTCAGGAGAAGAATGACCTGGCCCTGCAGCTGCAGGCAGAGCAGGACAACCTAGCGGATGCGGAGGAG CGCTGCCACTTGCTCATCAAGTCCAAGGTGCAGCTCGAGGCGAAGGTGAAGGAGCTGAACGAGCGGCTGG AGGATGAGGAGGAAGTGAACGCTGACCTGGCCGCACGCAGGCGCAAGCTGGAGGATGAGTGCACGGAGCT CAAGAAGGACATTGATGACCTGGAGCTGACCCTGGCCAAGGCTGAGAAGGAGAAACAAGCCACCGAGAAT AAGGTGAAGAACCTGACAGAGGAGATGGCTGCCCTGGACGAGTCCGTGGTCCGCCTGACTAAGGAGAAGA AGGCGTTGCAGGAGGCTCACCAGCAGGCTCTGGGTGACCTGCAGGCTGAAGAGGACCGTGTGAGTGCGCT GGCCAAGGCCAAGATCCGGTTGGAGCAGCAAGTAGAGGACCTGGAGTGCTCCCTGGAGCAAGAGAAGAAG CTTCGCATGGATACAGAACGTGCCAAGCGCAAGCTCGAAGGAGACCTGAAGCTGACGCAGGAGACAGTGA CAGACACCACACAAGACAAGCAGCAGCTGGAGGAGAAGTTGAAGAAGAAGGATTCAGAGCTGAGTCAGCT GAGCCTGCGGGTGGAGGATGAGCAGCTCCTGGGGGCGCAGTTGCAGAAGAAGATCAAGGAGCTACAGGCG AGGGCTGAGGAGCTGGAGGAGGAGCTGGAGGCGGAGCGAGCAGCCCGTGCCCGTGTGGAAAAGCAGCGAG CTGAGGCAGCCCGAGAGCTGGAGGAGCTGAGTGAGAGGCTAGAGGAGGCTGGTGGAGCCTCTGCCGGGCA GCGTGAGGGTTGCCGCAAGCGTGAGGCTGAGTTGGGCCGACTGCGGCGGGAGCTGGAGGAGACAGTTCTG AGACACGAGGCCACTGTGGCTGCCCTGCGCCGCAAGCAGGCTGATAGCGCCGCAGAGTTGAGCGAGCAGG TAGACAGCCTCCAGCGGATCCGGCAGAAACTGGAGAAGGAAAAGAGTGAGCTGCGCATGGAGGTGGATGA TCTGGGGGCCAGCGTGGAAACTCTGGCCCGTGGCAAGGCCAGTGCAGAGAAGCTGTGCCGGACCTATGAG GACCAGCTCAGCGAGGCCAAGATCAAAGTGGAGGAGCTGCAGCGGCAGCTGACTGACGCCAGCACCCAGC GGGGGCGGTTACAAACGGAGAACGGGGAGCTGGGCCGCCTGCTTGAGGAGAAGGAGTCTATGATCAGCCA GCTGAGCCGCGGGAAGACCTCAGCCGCTCAGAGTCTGGAGGAACTTCGGCGGCAGTTGGAGGAAGAAAGC AAGGCCAAGGGTGCGCTGGCTCACGCAGTGCAGGCTCTGCGGCACGACTGCGACCTCCTGCGAGAGCAGC ATGAGGAAGAGTCCGAGGCGCAGGCTGAGCTGCAGCGGCTGCTGTCCAAAGCCAACGCTGAGGTGGCACA GTGGAGGAGCAAGTATGAGGCGGACGCCATCCAAAGGACGGAGGAGCTGGAGGAGGCCAAGAAGAAGCTG GCCCTGAGGCTGCAGGAGGCAGAAGAAGGCGTAGAGGCTGCGAATGCCAAGTGCTCCTCACTGGAGAAGG CCAAGCTGCGGCTGCAGACAGAGTCCGAGGACGTGACCTTGGAGCTGGAGCGGGCCACCTCAGCCGCTGC TGCACTGGATAAGAAGCAGCGGCACTTGGAGCGGGCCCTGGAGGAACGGAGGCGGCAGGAGGAGGAGATG CAGCGGGAACTGGAGGCGGCGCAGAGGGAGGCTCGAGGTCTGGGCACCGAGCTCTTTCGATTGCGGCACA GCCATGAGGAGGCGCTTGAGGCTCTGGAGACACTCAAGCGGGAGAACAAGAATCTGCAGGAGGAGATCAG CGACCTTACAGACCAGGTCAGCCTCAGCGGGAAGAGCATCCAGGAACTGGAGAAGGCCAAGAAGGCGCTG GAAGGGGAGAAGAGTGAGCTGCAGGCTGCGCTGGAGGAGGCTGAGGGGGCGCTGGAGCTGGAAGAGACAA AGACCCTGCGGATCCAGTTGGAGTTGTCCCAGGTCAAGGCGGAAGTGGACCGAAAGCTGGCGGAGAAGGA TGAGGAGTGTACTAACCTGAGGCGCAACCACCAGCGGGCTGTGGAGTCCTTGCAGGCCTCCCTGGATGCC GAGACTAGGGCCCGCAATGAAGCACTGCGTCTCAAGAAGAAGATGGAGGGGGACCTCAATGACCTGGAGT TACAGCTGGGCCACGCAACCCGCCAGGCCATGGAGGCACAGGCAGCCACGAGGCTGCTTCAGGCCCAACT CAAGGAGGAGCAAGCAGGGCGTGATGAGGAGCAGAGGCTGGCTGCCGAGCTCCGCGAGCAGGGCCAGGCC CTCGAGCGCAGGGCTGCACTGCTGGCATCTGAGCTGGAAGAGCTTCGGGCCGCCCTGGAACAGGGCGAGC GCAGCCGAAGGCTGGCTGAACAGGAGCTGTTGGAGGCCACAGAGCGCCTCAACCTACTGCATTCACAGAA CACAGGCCTCCTGAACCAGAAAAAGAAGCTGGAGGTGGACTTGGCCCAGCTGAGTGGGGAGGTGGAGGAG GCTGCGCAGGAGAGGCGGGAAGCTGAGGAGAAGGCCAAAAAGGCCATCACGGATGCAGCCATGATGGCTG AGGAGCTGAAAAAGGAGCAGGACACAAGTGCACACCTCGAACGGATGAAGAAGACCCTGGAACAGACAGT GCGGGAGCTGCAGGCCCGCCTTGAGGAAGCAGAGCAGGCCGCACTCCGGGGTGGGAAGAAGCAAGTGCAG AAGCTGGAAGCCAAGGTGCGGGAACTGGAGGCAGAGCTTGACGCAGAGCAGAAGAAGCACGCTGAGGCCC TCAAGGGTGTGCGCAAACATGAGCGCAGGGTCAAGGAGCTTGCGTACCAGACTGAGGAAGACAGGAAGAA CCTGGCTCGCATGCAGGACCTAGTGGACAAGCTGCAGAGCAAGGTCAAGAGCTACAAGCGTCAGTTTGAG GAGGCGGAGCAGCAGGCCAGTACCAACCTGGCTAAGTACCGCAAGGCCCAGCACGAGTTAGACGACGCTG AGGAGCGGGCAGACATGGCAGAGACCCAGGCCAACAAGCTGCGGGCAAGGTCCAGGGATGCCCTGGGTCC CAAGCACAAGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107794
Insert Size	5826 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107794.2, NP_001101264.2
RefSeq Size	6097 bp
RefSeq ORF	5826 bp
Locus ID	311570

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