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Laptm4b (NM_001013174) Rat Untagged Clone
CAT＃: RN204081
Laptm4b (untagged ORF) - Rat lysosomal protein transmembrane 4 beta (Laptm4b), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN204081 representing NM_001013174
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGGTCGCGCCCTGGACTCGGTTCTATTCCCACAGCTGCTGCCTGTGCTGCCATGTCCGCACCG GCACCATCCTGTTGGGCATCTGGTACCTGATCATCAATGCCGTGGTCCTGTTGATCCTGCTGAGTGCCCT GGCCGATCCCGATCAGTATCACTTTTCAGGCTCTGAACTAGGAGGGGAATTTGAGTTCATGGACGATGCC AACATGTGCATTGCTATCGCGATCTCTCTCCTCATGATCCTGATCTGTGCCATGGCTACCTACGGTGCAT ACAAGCAACACGCTGCCTGGATCATCCCGTTCTTCTGTTACCAGATCTTTGATTTCGCCCTGAATACTTT GGTTGCAATCACCGTGCTTGTCTACCCCAACTCCATTCAGGAGTACATAAGACAGCTGCCCCCCAGCTTT CCCTACAGAGATGACATAATGTCAGTGAACCCTACCTGTTTGGTCCTTGTCATCCTTCTCTTCATAGGCA TTATTTTGACATTTAAGGGCTACTTGATCAGCTGTGTTTGGAGCTGCTACAGGTACATCAATGGCAGGAA CTCCTCTGATGTTCTGGTTTACGTTACCAGCAACGATACCACGGTGCTGTTGCCACCATACGATGATGCC ACTGCTGTGACCGGCACTGCCAAGGAGCCACCGCCACCTTACGTGTCTGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001013174 |
| Insert Size | 684 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001013174.1, NP_001013192.1 |
| RefSeq Size | 1750 bp |
| RefSeq ORF | 684 bp |
| Locus ID | 315047 |
| UniProt ID | Q5U1W4 |
| Gene Summary | Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR204081 | Laptm4b (Myc-DDK-tagged ORF) - Rat lysosomal protein transmembrane 4 beta (Laptm4b), (10 ug) |
CNY 3,990.00 |
|
| RR204081L3 | Lenti ORF clone of Laptm4b (Myc-DDK-tagged ORF) - Rat lysosomal protein transmembrane 4 beta (Laptm4b), (10 ug) |
CNY 6,080.00 |
|
| RR204081L4 | Lenti ORF clone of Laptm4b (mGFP-tagged ORF) - Rat lysosomal protein transmembrane 4 beta (Laptm4b), (10 ug) |
CNY 6,650.00 |
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