Polg (NM_053528) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204058 representing NM_053528 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGCCTGCTCTGGAAGAAGGTGGCCGGCGCCAAAGTCGCCTCAGGGCCAGTACCAGCAACAGGAC GCTGGGTCTCCAGCTCCGTCCTCGACCCCGTCCCCAGCGACGGGCAGCCGCAGTCGCAAATGCCCTCCTC AGAGAATGGGCAGCTGCGGCTCAACCCTCTGCACATCCAGATGCTGTCGAGAGGCCTGCACGAGCAAATC TTCGGGTGCGGAGGGGACGTGCCCGACGAGGCCGCGGTGCAGCGCAGCATCGAGCACCTACGGAAGCACG GGCTCTGGGGGCAGCCGACCACGCCCTTGCCAGACGTGCAGCTGCGCCTGCCCCGGCTCTTCGGGGGCAA CCTGGACCAGCACTTCCGCCTCCTGGCCCAGAAGCAGAGCCTCCCGTACTTGGAGGCGGCCGCCTCGTTA TCGGAGGCCCAATTGCCCCCCCAGCCCAGGAAGTGGGTCTGGGCGGAGGGCTGGACCCGGTACGGCCCCG AGGGGGAGGCCGAACCCGTGGCCATCCCCGAGGAGCGGGCCCTGGTGTTCGACGTGGAGGTCTGCTTGGC AGAGGGAACCTGCCCCACTTTGGCGGTGGCCATATCCCCCTCGGCCTGGTATTCCTGGTGCAGCCGGCGG CTGGTGGAAGAGCGTTACTCTTGGACCAGCCAGCTGTCGCCGGCTGACCTAATCCCTTTGGGGGTCTCTG CTAGTGCCAGCAGCTCCACCCAGCAGGATTGGCAGGAACAGTTAGTGGTGGGGCACAATGTTTCCTTTGA CCGAGCCCATATCAGGGAGCAGTATCTGATTCAGGGCTCACGCATGCACTTTCTGGACACTATGAGCATG CACATGGCTATCTCAGGGCTGAGCAGCTTCCAGCGCAGTCTGTGGATGGGAGCCAAGCAGGGCAAGCACA AGACCCAGCATCCTACAAAGCGAGGTCAGAAGTCCCAGAAGAATGCGAATGGTCCAGCGATTTCATCTTG GGACTGGATGGACATCAGCAGTGCTAATAATCTTGCAGACGTGCACAACCTTTATGTGGGGGGACCTCGC TTAGCGAAGGAGCCTCGGGAACTGTTCGTCAAAGGCAGCATGAGGGATATCAGGGAGAACTTCCAGGATC TGATGGAGTACTGTGCTCGTGATGTGTGGGCCACCTTTGAGGTTTTCCAGCAGCAGCTGCCGCTCTTCTT GGAGAGGTGTCCCCACCCAGTGACTCTGGCTGGCATGCTGGAGATGGGTGTCTCCTACCTGCCTGTCAAC CAGAACTGGGAGCGGTACCTGACAGAGGCACAGAGCACATATGAGGAACTCCAGCGGGAGATGAAGAAGT CGCTGATGGAGCTGGCTAATGATGCCTGCCAGTTGCTCTCAGGAGAGAGGTACAAAGAAGACCCTTGGCT CTGGGACCTAGAATGGGACTTGCAGGAATTTAAGCAGAAAAAGGCAAAGAAGGTGAAGAAGACAGCCTCA GCCAGCAAGTTGCCCATCGAGGGAGCTGGGCCCTTTGGGGATCCCATGGATCAGGAAGATCCTGGACCAC CCAGTGAGGAGGAGGAGCTTCAGCAAAATATAATGGCCCACACCCGTTTACAGCAGCTGAAGAGCACCAC AGACCTCCTGCCTAAGCGACCCCAACACCTTCCTGGACACCCTGGGTGGTACCGGAAACTCTGCCCTCGG CTAGATGACCCTGCATGGACTCCGGGCCCCAGCCTCCTCAGCCTGCAAATGCGGGTCACTCCTAAGCTCA TGGCACTGACCTGGGATGGTTTTCCTCTACACTACTCAGACTCTCATGGTTGGGGCTACCTGGTGCCTGG GCGGCGGGATAATCTGACTGAGCTGCCAGTGAGCCCCACTGAGGAGTCAGCTGCGGTGACCTGCCCCTAC AGAGCCATCGAGTCCTTGTACAGGAGGCACTGTCTTGACCAGGGCAAGCAGCAGCTGGAGACCCAGGAGA CAGACCTGGCTGAGGAGTTCTTGCTCACGGACAGTGCCATGTGGCAGACGGTAGAAGAACTGGGCTGCTT AGATGTGGAGGCTGAGGCCACAGTGGAGAGTTCAGGGCTGAGTCAGCCCCTAGTTCCGCCTACTGCTTGT GCCCCCAAAACCAGCCAGCCCACTTATCACCATGGCAACGGACCTTATAATGATGTGGATATCCCCGGTT GCTGGTTTTTCAAGCTGCCTCACAAGGATGGTAACAACTACAATGTGGGTAGTCCCTTTGCCAAAGATTT CCTGCCCAAGATGGAAGACGGCACTCTGCAGGCTGGCCCAGGGGGTGCCAGGGGACCTCGTGCCCTGGAA ATAAATAAAATGATTTCTTTTTGGAGGAATGCTCATAAACGCATCAGCTCCCAGATGGTGGTATGGCTCC CCAGGTCAGCTCTGCCCCGGGCTGTGACCAGGCACCCCAGCTTTGATGAGGAAAGCCACTATGGGGCCAT CCTGCCCCAGGTGGTGACTGCTGGCACCATCACCCGTCGCGCTGTGGAGCCCACGTGGCTCACTGCCAGC AATGCTCGGCCTGACCGGGTAGGCAGTGAGTTGAAAGCCATGGTGCAGGCTCCACCTGGCTATGTCCTTG TGGGTGCTGATGTGGACTCACAGGAACTGTGGATCGCAGCCGTTCTTGGAGATGCTCACTTTGCTGGGAT GCATGGCTGCACGGCCTTTGGCTGGATGACTCTTCAGGGCAGGAAGAGCAGAGGCACTGATCTGCACAGT AAGACAGCCGCCACTGTGGGCATCAGTCGTGAGCACGCCAAAGTCTTCAACTACGGCCGCATCTACGGGG CTGGGCAGTCCTTTGCCGAGCGCCTGCTGATGCAGTTCAACCACAGGCTCTCAAGGCAGGAGGCAGCTGA CAAGGCCCAGCAGATGTACGCTGTCACGAAAGGCCTGCGCCGATACCGGCTCTCAGATGATGGTGAATGG CTGGTGAAACAGTTAAATGTTCCTGTGGACAGGACAGAAGACGGCTGGGTTTCCCTACAAGATCTTCGAA AGATCCGAAGAGAAGCTTCAAGGAAGTCTCGATGGAAGAAGTGGGAGGTAGTCACTGAACGAGCATGGAC AGGGGGTACGGAGTCAGAAATGTTTAATAAGCTGGAGAGCATTGCCATGTCTGACACACCACGCACCCCA GTGCTGGGCTGCTGCATCAGCAGAGCCTTGGAGCCCTCGGTTGTCCAGGGAGAGTTTATGACCAGTCGTG TGAATTGGGTGGTACAGAGCTCTGCTGTGGACTACTTACACCTCATGCTTGTGGCCATGAAGTGGCTGTT TGAGGAATTTGCCATTGATGGGCGCTTCTGCATCAGCATCCATGATGAGGTGCGCTACCTGGTACGTGAG GAGGACCGCTACCGTGCTGCCCTGGCACTGCAGATCACCAATCTCCTGACCAGGTGCATGTTTGCCTACA AGCTGGGTCTGAATGACCTGCCCCAGTCAGTTGCCTTTTTCAGTGCAGTAGACATTGACCAGTGCCTCAG GAAGGAAGTGACCATGGACTGTAAAACTCCTTCTAACCCAACTGGGATGGAAAGGAAATACGGGATTCCC CAGGGTGAAGCACTGGATATTTACCAGATAATTGAACTCACCAAAGGCTCCTTGGAAAAACGAAGCCAGC CTGGACCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053528
Insert Size	3651 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053528.1, NP_445980.1
RefSeq Size	3651 bp
RefSeq ORF	3651 bp
Locus ID	85472
UniProt ID	Q9QYV8
Gene Summary	exhibits DNA polymerase activity; may mediate the final step of mitochondrial DNA repair [RGD, Feb 2006]

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