Polq (NM_001105878) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204023 representing NM_001105878 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCTTCCGCGCCGGAGTGGGAAACGGCGGCGTTCATCATCCGGCTCCGACTCATTCTCGTTCTCGG GAGACGGTGATAGCTGTGTCAGCCCTCAGCTCCTGTGTAGGCCGGTGCTGAGTCCACCTCCGGGGCTGGG ACGCGGCCGGAGGCTCGCAGGGACAGGAACATGCAAGCAAAGAGTTTCTGATGACCAGATAGACCAGCTG CTATTGGCAAACTGGGGACTCCCTAAAGCAGTTCTGGAGAAATACCACAATTTTGGTGTAAAAAAGATGT TCGAATGGCAGGCAGAGTGTCTTTTGCTCGGACAAGTCCTGGAAGGAAAGAATCTGGTTTATTCAGCTCC TACAAGTGCTGGGAAGACGCTTGTTGCAGAGTTACTTATTTTGAAGCGGGTTTTGGAAACGCGGAAGAAA GCGTTGTTCATTCTGCCCTTTGTGTCTGTGGCTAAAGAGAAGAAATACTATCTCCAGAGTCTGTTTCAGG AAGTAGGAATAAAGGTAGATGGCTATATGGGCAGCACCTCACCAACTGGACGTTTCTCCTCCTTGGACGT TGCTGTTTGCACTATTGAGAGAGCCAATGGGCTGATCAATCGCCTCATTGAGGAAAATAAGATGGATCTC TTAGGAACGGTGGTCGTGGATGAGCTACACATGCTGGGGGACTCTCACCGAGGGTATCTGCTGGAACTCT TACTGACCAAGGTTTGCTTTGTCACTCGGAAGTCAGCATCGTGCCAGGCAGATTCGGCCAGTGCTCTGGC TTGTGCTGTGCAGATTGTTGGCATGAGTGCTACTCTTCCTAACCTGCAGCTCGTGGCTTCCTGGCTGAAT GCTGAACTTTACCATACTGACTTTCGCCCTGTTCCACTTTTGGAATCAATAAAAGTAGGAAATTCCATAT ATGACTCTTCAATGAAGCTTGTGAGAGAATTTCAACCCCTGCTCCAAGTGAAGGGAGATGAAGACCACAT TGTTAGTTTATGCTATGAGACGGTTCGGGATAACCATTCAGTATTAGTCTTTTGTCCATCAAAAAAATGG TGTGAGAAGGTGGCCGATATCATTGCCCGGGAGTTTTATAATCTGCACCATCAACCCGAGGGATTAGTAA AGTCATCTGAATTTCCACCAGTGATTCTAGACCAAAAGAGCCTTCTTGAAGTGATAGATCAGTTAAAACG CTCACCTTCAGGACTAGACTCGGTGCTGAAGAATACTGTGCCATGGGGAGTAGCGTTTCATCATGCAGGT CTTACTTTTGAAGAGAGGGATATCATCGAAGGAGCCTTCCGTCAAGGTCTTATTCGCGTTTTGGCAGCAA CGTCTACTCTTTCTTCTGGAGTGAACCTTCCTGCACGTCGGGTGATTATTCGCACTCCGGTTTTCGGTGG TCAGCCTTTAGATATCCTCACTTATAAGCAGATGGTTGGTCGTGCTGGCAGGAAAGGAGTAGACACAATG GGTGAAAGTATACTAGTTTGTAAGAACTCTGAGAAATCCAAGGGCATTGCTCTGCTTCAAGGTTCGCTGG AGCCTGTGCACAGCTGTCTGCAAAGCCAGGGAGAAGTCACTTCTACCATGATACGAGCTATTCTGGAGAT AATCGTCAGTGGGGTGGCAAGTACGTCACAAGATATGCAGACTTATGCTGCCTGCACATTCTTGGCTGCT GACGTAAAGGAAGGGAAGCAAGGGATTCAGAGGAACCGAGATGATGTTCAGCGTGGGGCAGTCGATGCCT GTGTGACGTGGCTGTTAGAAAACGAGTTCATCCAGGCGGCAGAGCCCAGTGACGGCACAGGAGGAAAGGT GTATCACCCAACACACCTTGGATCAGCTACTCTTTCTTCCTCACTTTCTCCAACTGATACCCTGGATATT TTTGCTGACCTCCAAAGAGCAATGAAAGGCTTTGTTTTAGAGAATGACCTTCATATCGTCTACCTGGTTA CACCTGTGTTTGAAGATTGGACTAGTATTGATTGGTATCGGTTTTTCTGTTTATGGGAAAAATTACCAAC TTCTATGAAAAGGGTGGCAGAGCTGGTTGGAGTGGAGGAAGGGTTCTTGGCTCGATGTGTGAAAGGAAAA GTAGTAGCCAGAACTGAGAGACAGCATCGACAAATGGCCATCCATAAAAGGTTTTTCACAAGCCTTGTGT TATTGGATTTAATCAGTGAAATTCCCTTAAAGGAAATTAACCAGAAATATGGATGTAATCGTGGGCAGAT TCAGTCTTTGCAACAGTCAGCTGCTGTCTATGCAGGAATGATTACGGTGTTTTCCAACCGTCTGGGCTGG CACAACATGGAGCTACTGCTTTCTCAGTTTCAGAAGCGTCTTACGTTTGGCATCCAGAGAGAGCTGTGTG ACCTGATCCGGGTATCCTCGCTCAACGCTCAGAGAGCCAGGTTCCTGTATGCCTCTGGCTTTCTCACCGT GGCGGACCTCGCCAGAGCTGACACTGTAGAGGTGGAGGCGGCTCTGAAGGACGCTCTGCCTTTCAAAAGT GCCCGGAAGGCAGTGGACGAAGAAGAGGAAGCAGCTGAAGAACGCCGGAGTATGCGCACTATTTGGGTGG CTGGCAAGAGCTTATCTGCAAGGGAAGCAGCAGCTTTAATAGTAGAAGAAGCCAAAGTGATTCTGCAGCA GGACTTAATTGAAATGGGAGTACAGTGGGGTCCACACTCACCCTTGAGTTCTAGCACACACTCACTGACC AGTGGGTCAGAAGTAAAGGAGCACACGTTCAAGTCCCAGACTAAGAGTTCTCATAAAAGGTTAGCATCAA AGAGCAGGAACAGCATGAGAGTGAGTGGTTCTAATGGTAAGCAGTCACCAGAGGCAGGGCAAGGCCTAGA CGAGTGCAGAGAGCGCCCAGACTCTCTCTGTAAGTTCCAGGGGAATCACGAGATCCAGACACCCTCCGTT TATAGGGCAAGAAAACGGACTTCTTTAGGTGTAAACAAAGAAATGCTTCGAACCTCTCTGAAGGAAGGAA AACCAAGCACTAAGGAAGTTCTTCAAACTCTCTCATTTGAGAAAACAAGAAAAGCTGCTTTGAGTTTTAG TTCAGAGCAGGCGAACAACAGTTTTCCATCTGGGAGAGACAGAAAGTATCGGAAAAAATCTTGGGGTAGC AGTCCCATGAGTGACTCTGTGATGCATAGAGATGATTTACAAGGACAGACTATGTGTAAATCTACTCTAT GTGAAGACCCACAGAAGTCCTTAGAGGAGCAGAATACAGAATACAGAAGTCCAGGGCTATTTGCAAAAAA TGTATCTTTTTGTGCCAAAGAAAAATGCAACAAAACCTCATTCCCCTTACAAATGCAGCAGCCTTGCTTA AGGAGAAAACCAGAAAGTGGTGCTGCCGTGGACCATTCCGTTGCGGTGTCTCAGAATAAAAATGTGGTGG AGCAACCCCCTGGTGCTCCTAGGGACAGAAGAGGATTGGCTGCTCATGGCAGAGCAGAGGTAAATGAGGT GCTCACAGAGAATGGTACAGAAAGCCAACTCCACGACACTCATCCTGTCAGCCAGTGCCTGGAAAACCAC AGTGAGAAGCAGACAAACACGTGCACCAGACAGAAGACATTGACAGAGGGGCAAGCTGGTATTAGTCATG TGACTAGAGGCTCAAATGATCTCACACCCATCAGATGTGAACGTTTAAAGCTTAACAGCAAAGAACATGA CTCAAATCCTTGTCCTCAAGCATTGGGAACTAACGCAGGCAGAACTGAGGCACCTCAGTCATCTGAAGCA CTTGGTCAAGCAGGAGGCCAGTGTGAAAATCTTCTAAATTCTCCTGGTATACAAGAAAAAACAAGTGCTC ATGCAACAAATAAAACTGAACATAGTCATGTTGCTAACCAAGCCTTCTGTGACTTTGGAGATAGCTTATA CTTAGACACTCAGTCAGAGGAAATAATTGAACAGATGGCAACTAAAAATGCCACGCAAGGAGCAGAAGCA GCAGGGATAACGGAGGAGGGCTCCGCCACTCAGAACGAGCCTCACAGCACTACAGGTGGCCAGCATATCC CAGGAGCAGCCAACACAGACCATGTGGACCGTAAGAACACAGAGTCTGTGAAAGAAAACCCCGAAAAGAA CATCGACAGAAGAACTCCACACAGCCTCATTTTCCATTCTCCAACACCTCAGGGGGGAAACAGTGCTTGC TTTAAAGAGAATGAGCATTCTGTTACTGATTCCCAGTTAAACAGTTTTCTTCAAGGGTTGGAAACACAGG ACAAGCCAATCATACCTCTGGCTCCTCAAATGAGAACTTCTACTGGTGTAGAGGAAGAAAGTCTGCCTGA AACCAGTTTGAATATGAGTGACAGTATACTATTTGACAGTTTTGGAGAAGATAGCTTTGGACAAAGACAG TCACTTGATGTAAAGGCAAAACAGCCCCTCCTTTCCGAAATGACACCAAACCATTTCCACAATCCTCCAT ACCCACAAGAAGACCCAGTTATGACACCACATATGAGCGAGCCTCAGGGTACTCTGGAGCGAATGGCTTG TCTCAGTGGTGAATCTATTATATTTTCAGAGATAGACTCTGCTCAGGTGATCGAAGCCTTAGACAATATG GCTGCATTTTACATGCAAGAAAACTGTAACCCAATAACTCTTAAAACAGAACCCAGAGATTTGGCAGCGC TGGGTAATGAGTGTCCCCAAGGAGAAGTGGTCAGAGGAGAACAACATGAAGGGTCCTCAAAGCCCAAATT CATGGAGATAAATCAAGATAATTCATTCACTTGGTCAGCAGCATCATTTAACTTAAGTCCAGAGCTGCAA AGGATTTTAGATAAAGTGTCTACTCCTCGAGAAAATGAAGAGCCCGAATTGATGCATGCAGACTTGTCTT GCTTTGAAGAAAACAGCACAGAGTCACATGAGAGACAGGACATGAATTCAGACTTGGGGACAGTTCAGAG AACTTCATTTCTCCCCAGTAATGGAGTTAAAAGCAGGACTGAAGGGCTAGAGAGCAAAGCCAAGCATGGT GGAGCCTCATCTGCCTTGCCTCATAAAGCTGCAGCTGATGACAACGGCCTCATTCCTCCTACACCCCTTC CTGCCTCTGCCTCTGCCTCTGCCTCTAAGCTAGCACTTCCAGAGATTCTCGGAACATCTGTAAAACATCA GAAAGCCAGTTGTTTATTTGACTCACCTTCAGATAATCAAAACCAGGATTTAAGTCAAGAGCTTAGAGAC AGCCTCAAGGACTCTGATGGTTCTGTTGTAGACACAAGTTTTTTTCTGCAGTCTCAGGATGGACTGCTGT TAACTCAAGCCTCATGCAGCTCAGAGAGCCTGGCCATAATTGACGTAGCGAGTGACCAGATTCTCTTTCA GACATTTGTGAAGGAGTGGCAGTGCCAAAAGCGCTTTTCCATCTCTCTAGCTTGTGAAAAGATGACAAGT TCGACGTCTTCCAAAACTGCTACCATAGGTGGAAGGTTGAAGCAAGTTAACTCACCACAGGAAGCCTCTG TTGAAGATGATGGGTTCCCTGTGCATGGCTCTGACTGTGCTGTGGTAGTTGGACTAGCAGTGTGCTGGGG TGGAAAGGATGCCTATTATTTGTCATTGCAGAAGGAACAAAAGCAGTCTGAAATGAGTCCTAGTTTGGCC CCACCACCTCTGGATGCAACACTAACTGTTAAGGAGAGGATGGAGTACCTTCAGTCCTGCTTGCAAAAGA AGTCTGACCAAGAACGGTCTGTTGTCACCTATGATTTCATCCAGACCTATAAAGTTCTCCTCTTGTCCTG TGGCATCTCACTGGAACCCAGCTATGAAGACCCCAAGGTGGCTTGCTGGCTTCTTGACCCGGATTCTAAG GAGCCAACTCTTCATAGCATAGTGACCAGTTTTCTCCCCCATGAGCTAGCACTCCTAGAAGGAATAGAGA CAGGCCCAGGAATTCAGAGCCTGGGGCTAAATGTGAACACAGACCATTCTGGGCGGTACAGAGCGAGTGT GGAGTCTGTTCTCATCTTCAACTCCATGAATCAACTCAACTCTATGTTGCAGAAGGAAAACCTTCACGAT ATTTTCTGCAAAGTGGAGATGCCCTCTCAGTACTGCCTGGCCTTGCTGGAACTAAATGGAATTGGCTTTA GTACTGCTGAGTGTGAAACTCAGAAGCATATCATGCAAGCCAAGCTGGACGCCATTGAGACTCAAGCCTA TCAACTCGCTGGCCACAGCTTCTCCTTTACAAGTGCAGATGACATCGCACAGGTTTTATTTTTGGAACTA AAGCTACCACCGAATGGAGAGATGAAAACACAAGGCGGCAGAAAGACTCTGGGTTCCACCAGAAGAGGGA CGGAGAGCGACCGCAAACTCAGGCTGGGAAGACGGTTCAGCACTAGTAAGGATATTTTAAATAAGTTAAA GGACTTGCATCCTTTACCAGGCCTGATACTGGAATGGAGAAGAATCAGTAATGCTATTACCAAAGTGGTC TTCCCCTTGCAGCGGGAAAAGCACCTGAACCCCTTTCTCAGAATGGAGAGGATCTATCCTGTATCACAGT CACACACGGCTACAGGACGGATAACCTTTACAGAACCCAATATCCAAAATGTGCCAAGAGATTTTGAGAT TAAAATGCCAACACTAGTAAGAGAGAGCCCACCTTCTCAAGCTTCAGGCAAGGGCCAGCTCGCGATGGCC AGACAAAATCAGAAGGTTTATGGCCTGCACCCGGGGCAAAGAACGGTGCTGGAGAAGACCTCAGATAGAG GCGTGCCATTTTCAGTGAGCATGCGCCATGCCTTTGTGCCTTTCCCAGGTGGTTTGATATTGGCTGCCGA CTACTCTCAGCTCGAACTGAGGATCCTGGCACACTTATCTCGTGACTGTCGTCTCATTCAAGTTTTAAAC TCAGGAGCGGATGTGTTCAGGAGCATTGCCGCTGAGTGGAAGATGATTGAGCCCGATGCTGTTGGTGACA ATCTGAGGCAACAGGCAAAGCAGATTTGCTATGGAATTATATATGGGATGGGAGCTAAATCGTTAGGAGA GCAGATGGGCATTAAAGAAAATGATGCTGCTTGCTATATCGACTCTTTCAAGTCCAGGTATAAAGGGATT AATCATTTCATGAGGGACACAGTGAAGAACTGTAGAAGAGATGGATTTGTTGAGACAATTTTGGGAAGAC GTAGATATTTGCCAGGAATCAAAGACAATAATCCTTATCATAAGGCACACGCTGAGCGTCAGGCCATCAA CACAACAGTTCAAGGCTCAGCTGCTGACATAGTCAAAGTAGCCACAGTTAACATCCAGAAGCAGCTAGAG ACCTTCCATCCAACCTTCAAATCCCATGGTCATCGGGAAAGCATGCTCCAGAGTGACCGAGCAGGATTGT TGCCAAAGAGAAAAGTTAAAGGGATGTTTTGTCCTATGAGAGGAGGATTCTTCATCCTTCAACTTCACGA CGAGCTTTTATATGAAGTGGCAGAAGAGGATGTTGTTCAGGTAGCTCAGATTGTCAAGAATGAAATGGAA TGTGCTATTAAACTTTCTGTGAAGCTGAAAGTGAAAGTGAAAATAGGTGCCAGCTGGGGGGAGCTGAAGG ACTTTGATGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001105878
Insert Size	7644 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001105878.1, NP_001099348.1
RefSeq Size	8527 bp
RefSeq ORF	7644 bp
Locus ID	288079

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