Ptgdr2 (NM_001012070) Rat Untagged Clone

CAT#: RN203997

Ptgdr2 (untagged ORF) - Rat G protein-coupled receptor 44 (Gpr44), (10 ug)



  "NM_001012070" in other vectors (3)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Gpr44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN203997 representing NM_001012070
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAACATCACACTGAAGCCGCTCTGTCCACTCTTGGAGGAGATGGTCCAGCTTCCAAACCACAGCA
ACTCTAGCCTCCGCTATATTGACCACGTGTCGGTGCTGTTGCACGGGCTGGCCTCCCTGCTGGGCCTGGT
GGAAAACGGACTCATCCTGTTTGTGGTGGGCTGTCGCATGCGCCAGACGGTGGTCACTACCTGGGTGCTG
CACCTGGCGCTGTCCGACTTGTTAGCCGCTGCCTCTCTGCCTTTCTTCACCTACTTTCTGGCAGTGGGCC
ACTCGTGGGAACTGGGCACCACTTTCTGCAAGCTACATTCCTCGGTCTTCTTCCTCAACATGTTTGCCAG
CGGCTTCCTGCTCAGCGCCATCAGCCTGGATCGCTGCCTGCAGGTGGTGAGGCCAGTGTGGGCACAGAAC
CACCGCACGGTGGCGGCCGCGCACAGAGTCTGCCTGATGCTCTGGGCTCTGGCGGTGCTCAACACAGTAC
CCTATTTCGTGTTCAGGGACACCATCCCGCGGCGTGATGGCCGCATCATGTGCTATTACAACATGCTGCT
CTTGAATCCAGGGTCTGACCGCGACACCACGTGCGACTACCGTCAGAAGGCCCTGGCGGTCAGCAAGTTC
CTGTTGGCCTTCATGGTACCTCTGGCCATCATCGCCTCGAGCCACGTGGCTGTGAGCCTACAACTGCATC
ACCGTGGTCGCCAGAGGACAGGCCGTTTCGTGCGTCTGGTGGCAGCCATAGTGGTGGCCTTCATACTCTG
CTGGGGGCCCTACCACATCTTCAGTCTGTTGGAGGCGCGTGCCCATTCTGTCACCACGCTACGGCAGCTC
GCGTCACGTGGGCTGCCCTTTGTCACCAGCCTGGCCTTCTTCAACAGCGTGGTCAACCCACTACTCTATG
TGCTCACTTGTCCCGACATGTTGCACAAGCTGAGGCGCTCGCTACTCACGGTGCTTGAAAGCGTGCTGGT
GGAGGACAGCGACCTGAGTACTGGGCCCGGCAAGCGCTGTCGCCGCCGCCATCGCCGCCGCGCCTCCTCC
ACCACCACCCCAGCCTCTACCTTACTGCTGGCTGACCGATTTCCCCAACTGCGTCCTGCTCGCTTAATCG
GCTGGATGAGGCGTGGCAGTGCAGAGCTCCCACGGAGGGTCCGAGAGCAGTCCCAGGAGAAGCAGGGCTC
ATTGAGCTGTACTTTGGATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001012070
Insert Size 1212 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001012070.1, NP_001012070.1
RefSeq Size 1297 bp
RefSeq ORF 1212 bp
Locus ID 309212
UniProt ID Q6XKD3
Gene Summary Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca(2+) mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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