Abca5 (NM_173307) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203757 representing NM_173307 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACTGCAATTAGGGATGTGGGCGTTTGGAGACAGACCAGAACACTTCTTCTGAAGAATTACCTCG TCAAATGCAGGACTAAAAAGAGTAGTGTTCAGGAAATTCTTTTTCCTCTATTTTTCTTATTTTGGCTGAT ATTAATTAGCATGATGCATCCAAATAAGAAATATGAAGAAGTATCTGATATAGAACTCAGCCCTATGGAC AAATCCATCCTTTCCAACCTTATCCTGGGGTACACTCCAGTGACTAATACTACAAGCAGCGTCATGCAGA GAGTTTCTACCGATCATCTTCCCGACGTTCTAGTTACTGAAGAATACGCCAGTGAGAAGGAGCTGCTGGC ATCCAGTTTGTCTAAGCCCAGCAACTTCGTGGGTGTGGTTTTCAAAGACGTCATGTCCTATGAACTGCGG TTTTTTCCTGATATGGTTCCAGTGTCTTCTGTTTATATGGATTCAAGAGCAGGCTGTTCAAAGTCATGTG ATGCTGCTCAGTACTGGTCTTCAGGGTTTACAGCTTTACAGGCCTCAATAGATGCTGCCATTATACAGTT GAAGACCAATGTTTCTCTGTGGAGGGAGCTGGAGTCAACCAAAGCTGTCATCATGGGGGAAGCTGCGGTC GTGGAGATTGACACCTTCCCACGAGGGGTCATCCTCATCTACCTCGTCATAGCCTTCTCGCCCTTCGGGT ACTTTCTGGCAATTCACATTGTAGCAGAAAAGGAAAAGAGGTTAAAGGAGTTTTTAAAGATAATGGGACT TCACGACACTGCTTTTTGGCTGTCCTGGGTTCTTCTGTACACGAGTTTGATCTTCCTTATGTCCCTTCTT ATGGCCGTCATTGCAACAGCGTCCTCGTTATTCCCTCAGAGTAGCAGCATCGTGATTTTTCTGCTCTTCT TCCTGTACGGATTGTCATCTGTGTTTTTTGCTTTAATGTTGACGCCTCTTTTTAAAAAATCAAAGCACGT GGGAGTCGTTGAGTTTTTTGTCACCGTGGTGTTTGGCTTTGTCGGCCTGTTGATAGTCCTCGTAGAAAGT TTCCCCAGGTCGCTAGTGTGGCTCTTCAGTCCTTTGTGTCAGTGCGCCTTTCTGATTGGGATTGCACAGG TCATGCATTTAGAAGATTTCAATGAAGGAGCCTTATTTTCTAGTTTGACTGAAGGCCCCTACCCGCTAAT TATCACCCTTACCATGCTCGCTCTGGACAGTGTGTTCTATGCCCTGCTGGCTGTGTACCTTGATCAGGTC ATCCCAGGGGAATTTGGCTTGAGGAGGTCATCTTTGTACTTCCTAAAGCCATCATATTGGTCAAAAAACA AAAGGAACTATAAGGAGCTATCAGAGGGCAACATCAATGGGAATATTAGTCTCAATGAAATTGTTGAGCC CGTTTCTTCAGAATTTATAGGAAAAGAAGCTATAAGAATAAGTGGTATTCAGAAAGCCTATAGGAAGAAG AATGAGACCGTGGAGGCTTTGAGAAATTTGTCGTTTGACATCTATGAAGGCCAGATTACTGCTCTGCTGG GCCACAGTGGAACAGGAAAAAGCACACTAATGAATATTCTGTGTGGACTGTGCCCACCTTCTGATGGGTT TGCTTCTATATATGGACACAGAGTCTCTGAAATAGATGAAATGTTTGAAGCGAGGAAAATGATTGGCATA TGTCCACAGTCAGATATGAACTTTGATGTTCTGACAGTAGAAGAAAATTTATCAATTTTGGCTTCAGTCA AAGGAATACCAGCCAACAACATAATTCAGGAAGTACAGAAAGTTTTACTGGATTTGGACATGCAAGCCAT CAAAGATAATCAAGCGAAAAAACTAAGTGGTGGCCAAAAGAGAAAGCTGTCTTTAGGAATTGCAGTTCTT GGGAATCCAAAGATCCTCCTGCTAGATGAGCCGACAGCAGGAATGGACCCCTGCTCTCGCCACATTGTTT GGAATCTTCTCAAGTACAGGAAGGCTAACCGAGTGACAGTCTTCAGCACTCACTTCATGGATGAGGCCGA CATTCTTGCCGACAGGAAAGCTGTCATATCACAAGGAATGCTGAAATGTGTTGGTTCTTCAATTTTCCTG AAAAGTAAATGGGGAATCGGCTACCGTCTGAGCATGTATATAGACAGGTACTGTGCCACAGAGTCGCTCT CGTCGCTGGTTAGACAGCACATCCCTGCGGCCGCTCTGCTGCAGCAGAATGACCAGCAGATTGTCTATAG CTTGCCCTTCAAAGACATGGACAAATTTTCAGGCTTGTTTTCTGCTCTAGACATTCATTCAAACCTGGGT GTAATTTCTTATGGTGTTTCCATGACGACATTGGAAGACGTGTTTTTAAAGCTAGAAGTTGAAGCAGAAA TTGACCAAGCAGATTATAGTGTATTTACCCAGCAGCCACGGGAGGAAGAAACAGATTCAAAGTCTTTTGA TGAAATGGAACAGAGTTTACTTATTCTCTCTGAAACCAAAGCTTCTCTAGTGAGCACCATGAGTCTCTGG AAGCAGCAAGTATCTACAATAGCAAAGTTTCATTTCCTCTCACTGAAGCGAGAAAGCAAATCAGTGCGCT CTGTGTTGCTTCTGCTTTTAATTTTTTTTGCAGTTCAGATTTTTATGTTTTTGGTCCATCATTCTTTTAA AAATGCTGTGGTTCCCATCAAGCTCGTTCCAGACTTGTATTTTCTAAAACCTGGAGATAAACCTCATAAA TACAAAACAAGCCTGCTGCTTCAAAATTCTACTGATTCAGATATCAATGATCTTATTGACTTTTTCACAC AGCAGAACATAATTGTGGCCATGTTTAATGACAGTGACTATGTGTCTGCTGCTCCTCATAGTGCGGCTCT CAATGTGGTGCAGTCAGAAAAGGACTATGTTTTTACTGCTGTTTTCAACAGTACTATGGTTTATTCTTTG CCAGTAATGATGAACATCATTAGTAACTACTATCTCTATCATTTAAATGTGACTGACACTATCCAGATCT GGAGTACCCCGTTCATTCAGGAAATTACTGACATTGTTTTTAAAGTTGAGCTATATTTTCAAGCAGCTTT GCTTGGAATCATTGTTACTGCAATGCCACCTTACTTTGCCATGGAAAATGCAGAGAATCATAAGATCAAA GCTTACACTCAGCTTAAACTTTCAGGTCTTTTGCCATCCGCCTACTGGATTGGACAGGCTGTGGTGGACA TTCCCCTGTTTTTTGTTGTTCTGACTTTGATGCTGGGAAGTTTATTTGCCTTTCATCACGGACTGTATTT TTATCCTGTAAAGTTTCTTGCTGTGGTTTTTTGCCTCATTGCTTATGTGCCCTCGGTTATTCTGTTCACT TATATAGCTTCGTTCACCTTTAAGAAAATTTTAAATACCAAGGAATTTTGGTCATTTATCTATTCTGTGA CAGCATTGGCTTGTGTCGCAGTCACGGAAATAACTTTCTTTCTGGGGTATGGAGTTACGGCTGTTTTTCA TTACACCTTCTGCATAGCCATCCCAATCTACCCGCTCCTGGGTTGTCTGATTTCTTTCATAAAGGGTTCT TGGAAGAATATACCAAAAACTGAGAATGCCTACAATCCCTGGGACAGACTTTTAGTTGCTGTAATCATGC CCTACCTGCAGTGTGTACTGTGGATTTTCCTCTTACAACACTATGAGAAAAAACATGGAGGCAGATCAAT AAGAAAGGATCCGCTTTTCAGGGCCCTCTCACAAAAAGCCAAACATAAGAAGTTTCCAGAACCACCCATC AATGAGGATGAAGATGAAGATGTCAAAGCGGAAAGGTTGAAGGTTAAAGAGCTGATGGGTTGCCAGTGTT GTGAGGAGAAACCGGCCATTATGGTATACAATTTGCATAAAGAATATGATGACAAGAAAGATTTCCTTCA CTCAAGGAAAACAACAAAAGTAGCAACAAAATACGTCTCTTTCTGTGTGAAAAAAGGAGAGATCTTGGGA CTGTTGGGTCCAAATGGTGCTGGCAAAAGCACGATTATTAATATTCTTGTTGGGGATGTTGAACCAACTT CAGGCAAGATATTTCTAGGAGATTATGGTTCACATTCAAATGAAGATGATGAATCCACCAAGTGTATGGG TTACTGCCCCCAGACAAACCCACTCTGGCCAGATATTACTCTGCAAGAACATTTTGAAATTTATGGAGCT GTGAAAGGGATGAGTTCAGGTGACATGAAGGAAGTAATCAGTCGAATAACAAAAGCCCTTGATTTGAAAG AACATCTTCAGAAAACTGTAAAGAAACTCCCTGCAGGGATCAAGCGAAAGCTGTGTTTTGCTCTCAGTAT GCTGGGGAATCCTCAGGTGACACTGCTGGATGAGCCGTCTACAGGTATGGACCCCAGAGCCAAACAGCAC ATGTGGCGAGCCATTCGAACTGCGTTTAAAAACAAGAAGCGGGCTGCCCTCCTCACTACGCATTACATGG AAGAGGCGGAGGCTGTCTGTGACAGGGTGGCCATCATGGTGTCCGGGCAGCTAAGATGCATTGGAACAGT ACAACATCTAAAGAGCAAGTTTGGCAAAGGCTACTTTTTAGAAATTAAACTAAAGGACTGGATAGAAAAC CTGGAAATAGATCGCCTTCAAAGAGAAATTCAATATATTTTCCCAAATGCAAGCCGCCAAGAGAGCTTCT CTTCTATTCTGGCTTATAAAATTCCAAAAGAAGATGTCCAGTCTCTCTCACAGTCCTTTGCTAAGTTGGA GGAAGCAAAACACACTTTTGCCATTGAAGAATACAGCTTTTCTCAAGCAACCCTGGAACAGGTTTTTGTA GAACTTACTAAGGAGCAGGAGGAGGAAGATAATAGCTGCGGGACTTTAAACAGCACGCTCTGGTGGGAAA GAAGGCAGGAAGACAGAGTCGTGTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_173307
Insert Size	4929 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_173307.1, NP_775429.1
RefSeq Size	5170 bp
RefSeq ORF	4929 bp
Locus ID	286970
UniProt ID	Q8CF82
Gene Summary	ATP-binding cassette (ABC) transporter [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.