Vcl (NM_001107248) Rat Untagged Clone

CAT#: RN203691

Vcl (untagged ORF) - Rat vinculin (Vcl), (10 ug)



  "NM_001107248" in other vectors (3)

CNY 17,100.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN203691 representing NM_001107248
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGTGTTTCACACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCGCAGCAGATCTCTCACCTGG
TGATTATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTGGCCGCTGT
GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGGAAAGAGACCGTTCAGACCACTGAGGATCAGATTTTG
AAGAGAGATATGCCGCCAGCATTTATTAAGGTCGAGAATGCTTGCACGAAGCTTGTTCAGGCAGCTCAGA
TGCTTCAGTCAGACCCATACTCTGTTCCTGCGCGGGATTACCTCATCGACGGCTCCAGGGGAATCCTCTC
TGGAACGTCTGACCTCCTGCTTACCTTTGATGAGGCTGAGGTTCGAAAAATTATAAGAGTTTGCAAAGGG
ATTTTGGAATACCTCACAGTGGCAGAGGTAGTGGAGACTATGGAAGACTTGGTCACTTACACAAAGAACC
TTGGGCCCGGAATGACGAAGATGGCCAAAATGATCGATGAGAGACAGCAGGAGTTGACGCACCAGGAACA
CCGTGTGATGCTGGTGAACTCAATGAACACTGTCAAAGAGCTGCTTCCTGTTCTCATTTCAGCTATGAAG
ATTTTTGTTACAACCAAAAACTCAAAAAACCAAGGAATAGAAGAAGCTCTGAAAAATCGAAATTTTACTG
TAGGAAAGATGAGTGCTGAGATTAACGAGATCATTCGTGTGTTACAACTCACCTCCTGGGATGAGGACGC
CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCGCTGGCCTCCATAGACTCGAAACTGAACCAGGCC
AAAGGTTGGCTCCGTGACCCCAACGCTTCCCCAGGGGACGCCGGTGAGCAGGCCATCAGGCAGATCTTGG
ACGAAGCTGGAAAAGTTGGTGAACTTTGTGCAGGCAAGGAACGCAGGGAGATCCTAGGAACCTGCAAGAT
GCTAGGGCAGATGACTGACCAAGTGGCTGACCTCCGAGCCAGAGGACAAGGAGCCTCGCCAGTGGCCATG
CAGAAGGCCCAGCAAGTGTCTCAGGGGCTCGACGTGCTTACTGCGAAAGTGGAAAATGCAGCTCGAAAGC
TGGAAGCCATGACGAACTCAAAGCAGAGTATTGCGAAGAAGATTGACGCTGCGCAGAATTGGCTGGCAGA
TCCCAATGGTGGACCTGAAGGAGAAGAACAGATCCGAGGGGCTTTGGCTGAAGCTCGGAAGATTGCTGAG
TTATGTGATGATCCTAAGGAGAGGGACGACATCCTCCGCTCTCTTGGAGAGATAGCGGCTCTGACCTCTA
AATTAGGAGACTTGCGAAGACAGGGGAAAGGAGACTCCCCAGAGGCTCGAGCCTTGGCTAAACAAGTGGC
AACCGCGCTACAGAACCTGCAGACCAAAACCAATAGGGCTGTGGCCAACAGCAGGCCTGCCAAGGCAGCC
GTCCACCTGGAAGGCAAGATTGAGCAGGCACAGCGGTGGATTGATAACCCTACAGTGGATGACCGAGGAG
TCGGTCAGGCTGCCATCCGTGGACTTGTGGCTGAAGGGCATCGGCTGGCCAATGTCATGATGGGACCTTA
TCGCCAAGATCTTCTCGCCAAGTGTGACCGTGTAGACCAGCTAGCAGCTCAGCTGGCTGACCTGGCTGCC
CGAGGGGAGGGGGAGAGTCCTCAGGCGAGAGCGCTTGCTTCTCAGCTTCAGGACTCCTTAAAGGATCTTA
AAACCCAGATGCAGGAAGCTATGACTCAAGAGGTGTCCGACGTTTTCAGCGATACTACAACTCCCATCAA
GCTGTTGGCAGTGGCGGCCACTGCTCCTCCTGATGCGCCCAATAGGGAAGAGGTATTTGACGAGAGAGCA
GCCAACTTTGAAAACCATTCAGGAAGGCTTGGGGCCACGGCAGAGAAGGCAGCTGCTGTTGGTGCTGCCA
ATAAATCGACAGTGGAAGGCATTCAGGCATCCGTGAAGACAGCCCGAGAACTCACTCCCCAGGTGATCTC
AGCTGCTCGAATCTTACTGAGGAACCCTGGTAACCAAGCTGCGTATGAACATTTTGAGACCATGAAGAAC
CAGTGGATTGATAATGTTGAGAAAATGACAGGGCTGGTGGACGAGGCCATTGATACCAAATCTCTGTTGG
ATGCTTCTGAAGAAGCAATTAAAAAAGACCTGGACAAGTGTAAGGTAGCCATGGCCAATATTCAGCCTCA
GATGCTGGTCGCTGGAGCAACCAGTATTGCTCGTCGGGCCAACCGGATTCTGCTGGTTGCTAAGAGGGAG
GTAGAGAACTCTGAGGATCCGAAGTTCCGAGAGGCTGTGAAAGCTGCCTCTGATGAACTGAGCAAAACAA
TCTCCCCCATGGTTATGGATGCCAAGGCTGTGGCTGGAAACATCTCTGACCCTGGCCTGCAAAAGAGCTT
CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAAGTTAGAGAAGCCTTCCAACCTCAGGAGCCT
GACTTCCCGCCTCCTCCACCAGACCTTGAACAGCTACGTCTAACTGATGAGCTGGCTCCTCCCAAACCAC
CTCTGCCTGAGGGTGAAGTCCCTCCACCCAGGCCACCCCCACCAGAAGAGAAGGATGAAGAGTTCCCTGA
GCAGAAAGCCGGGGAGGTGATTAACCAGCCGATGATGATGGCCGCCAGGCAGCTCCACGATGAAGCTCGG
AAGTGGTCTAGCAAGGGCAATGACATCATCGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCGGAGATGT
CTCGGCTGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTTATTCAGTGTGCCAAGGATATCGCCAAGGC
CTCTGATGAGGTGACGAGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAGCGGATTAGAACCAAT
CTCTTACAGGTCTGTGAGCGAATCCCAACTATAAGCACCCAGCTCAAAATCCTGTCCACAGTGAAGGCCA
CCATGCTGGGCCGGACCAACATCAGTGACGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCATAATGC
CCAGAACCTCATGCAGTCTGTGAAAGAGACTGTGCGAGAGGCCGAAGCTGCTTCAATCAAGATTCGAACA
GACGCCGGATTTACTCTGCGCTGGGTCAGAAAGACTCCCTGGTACCAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001107248
Insert Size 3201 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001107248.1, NP_001100718.1
RefSeq Size 5270 bp
RefSeq ORF 3201 bp
Locus ID 305679
UniProt ID P85972
Gene Summary Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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