Vcl (NM_001107248) Rat Untagged Clone
CAT#: RN203691
Vcl (untagged ORF) - Rat vinculin (Vcl), (10 ug)
CNY 17,100.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN203691 representing NM_001107248
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGTTTCACACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCGCAGCAGATCTCTCACCTGG TGATTATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTGGCCGCTGT GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGGAAAGAGACCGTTCAGACCACTGAGGATCAGATTTTG AAGAGAGATATGCCGCCAGCATTTATTAAGGTCGAGAATGCTTGCACGAAGCTTGTTCAGGCAGCTCAGA TGCTTCAGTCAGACCCATACTCTGTTCCTGCGCGGGATTACCTCATCGACGGCTCCAGGGGAATCCTCTC TGGAACGTCTGACCTCCTGCTTACCTTTGATGAGGCTGAGGTTCGAAAAATTATAAGAGTTTGCAAAGGG ATTTTGGAATACCTCACAGTGGCAGAGGTAGTGGAGACTATGGAAGACTTGGTCACTTACACAAAGAACC TTGGGCCCGGAATGACGAAGATGGCCAAAATGATCGATGAGAGACAGCAGGAGTTGACGCACCAGGAACA CCGTGTGATGCTGGTGAACTCAATGAACACTGTCAAAGAGCTGCTTCCTGTTCTCATTTCAGCTATGAAG ATTTTTGTTACAACCAAAAACTCAAAAAACCAAGGAATAGAAGAAGCTCTGAAAAATCGAAATTTTACTG TAGGAAAGATGAGTGCTGAGATTAACGAGATCATTCGTGTGTTACAACTCACCTCCTGGGATGAGGACGC CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCGCTGGCCTCCATAGACTCGAAACTGAACCAGGCC AAAGGTTGGCTCCGTGACCCCAACGCTTCCCCAGGGGACGCCGGTGAGCAGGCCATCAGGCAGATCTTGG ACGAAGCTGGAAAAGTTGGTGAACTTTGTGCAGGCAAGGAACGCAGGGAGATCCTAGGAACCTGCAAGAT GCTAGGGCAGATGACTGACCAAGTGGCTGACCTCCGAGCCAGAGGACAAGGAGCCTCGCCAGTGGCCATG CAGAAGGCCCAGCAAGTGTCTCAGGGGCTCGACGTGCTTACTGCGAAAGTGGAAAATGCAGCTCGAAAGC TGGAAGCCATGACGAACTCAAAGCAGAGTATTGCGAAGAAGATTGACGCTGCGCAGAATTGGCTGGCAGA TCCCAATGGTGGACCTGAAGGAGAAGAACAGATCCGAGGGGCTTTGGCTGAAGCTCGGAAGATTGCTGAG TTATGTGATGATCCTAAGGAGAGGGACGACATCCTCCGCTCTCTTGGAGAGATAGCGGCTCTGACCTCTA AATTAGGAGACTTGCGAAGACAGGGGAAAGGAGACTCCCCAGAGGCTCGAGCCTTGGCTAAACAAGTGGC AACCGCGCTACAGAACCTGCAGACCAAAACCAATAGGGCTGTGGCCAACAGCAGGCCTGCCAAGGCAGCC GTCCACCTGGAAGGCAAGATTGAGCAGGCACAGCGGTGGATTGATAACCCTACAGTGGATGACCGAGGAG TCGGTCAGGCTGCCATCCGTGGACTTGTGGCTGAAGGGCATCGGCTGGCCAATGTCATGATGGGACCTTA TCGCCAAGATCTTCTCGCCAAGTGTGACCGTGTAGACCAGCTAGCAGCTCAGCTGGCTGACCTGGCTGCC CGAGGGGAGGGGGAGAGTCCTCAGGCGAGAGCGCTTGCTTCTCAGCTTCAGGACTCCTTAAAGGATCTTA AAACCCAGATGCAGGAAGCTATGACTCAAGAGGTGTCCGACGTTTTCAGCGATACTACAACTCCCATCAA GCTGTTGGCAGTGGCGGCCACTGCTCCTCCTGATGCGCCCAATAGGGAAGAGGTATTTGACGAGAGAGCA GCCAACTTTGAAAACCATTCAGGAAGGCTTGGGGCCACGGCAGAGAAGGCAGCTGCTGTTGGTGCTGCCA ATAAATCGACAGTGGAAGGCATTCAGGCATCCGTGAAGACAGCCCGAGAACTCACTCCCCAGGTGATCTC AGCTGCTCGAATCTTACTGAGGAACCCTGGTAACCAAGCTGCGTATGAACATTTTGAGACCATGAAGAAC CAGTGGATTGATAATGTTGAGAAAATGACAGGGCTGGTGGACGAGGCCATTGATACCAAATCTCTGTTGG ATGCTTCTGAAGAAGCAATTAAAAAAGACCTGGACAAGTGTAAGGTAGCCATGGCCAATATTCAGCCTCA GATGCTGGTCGCTGGAGCAACCAGTATTGCTCGTCGGGCCAACCGGATTCTGCTGGTTGCTAAGAGGGAG GTAGAGAACTCTGAGGATCCGAAGTTCCGAGAGGCTGTGAAAGCTGCCTCTGATGAACTGAGCAAAACAA TCTCCCCCATGGTTATGGATGCCAAGGCTGTGGCTGGAAACATCTCTGACCCTGGCCTGCAAAAGAGCTT CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAAGTTAGAGAAGCCTTCCAACCTCAGGAGCCT GACTTCCCGCCTCCTCCACCAGACCTTGAACAGCTACGTCTAACTGATGAGCTGGCTCCTCCCAAACCAC CTCTGCCTGAGGGTGAAGTCCCTCCACCCAGGCCACCCCCACCAGAAGAGAAGGATGAAGAGTTCCCTGA GCAGAAAGCCGGGGAGGTGATTAACCAGCCGATGATGATGGCCGCCAGGCAGCTCCACGATGAAGCTCGG AAGTGGTCTAGCAAGGGCAATGACATCATCGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCGGAGATGT CTCGGCTGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTTATTCAGTGTGCCAAGGATATCGCCAAGGC CTCTGATGAGGTGACGAGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAGCGGATTAGAACCAAT CTCTTACAGGTCTGTGAGCGAATCCCAACTATAAGCACCCAGCTCAAAATCCTGTCCACAGTGAAGGCCA CCATGCTGGGCCGGACCAACATCAGTGACGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCATAATGC CCAGAACCTCATGCAGTCTGTGAAAGAGACTGTGCGAGAGGCCGAAGCTGCTTCAATCAAGATTCGAACA GACGCCGGATTTACTCTGCGCTGGGTCAGAAAGACTCCCTGGTACCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001107248 |
Insert Size | 3201 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001107248.1, NP_001100718.1 |
RefSeq Size | 5270 bp |
RefSeq ORF | 3201 bp |
Locus ID | 305679 |
UniProt ID | P85972 |
Gene Summary | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.[UniProtKB/Swiss-Prot Function] |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR203691 | Vcl (Myc-DDK-tagged ORF) - Rat vinculin (Vcl), (10 ug) |
CNY 17,100.00 |
|
RR203691L3 | Lenti ORF clone of Vcl (Myc-DDK-tagged ORF) - Rat vinculin (Vcl), (10 ug) |
CNY 18,720.00 |
|
RR203691L4 | Lenti ORF clone of Vcl (mGFP-tagged ORF) - Rat vinculin (Vcl), (10 ug) |
CNY 20,520.00 |