Sf3b3 (NM_001106187) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203604 representing NM_001106187 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCTGTACAACTTGACCTTACAGCGGGCCACTGGCATCAGCTTTGCCATCCATGGCAACTTCTCTG GAACCAAACAACAGGAAATTGTCGTTTCCCGTGGGAAGATTTTGGAGTTACTTCGCCCAGATCCTAACAC TGGCAAAGTGCATACCCTTCTCACTGTTGAAGTTTTTGGTGTTATCCGGTCACTTATGGCATTTAGGCTA ACAGGTGGCACCAAAGACTACATTGTAGTTGGCAGTGACTCAGGCCGAATTGTTATCTTGGAATACCAGC CATCAAAGAATATGTTTGAGAAAATTCACCAAGAAACTTTCGGCAAAAGTGGATGTCGCCGTATTGTTCC TGGCCAGTTCTTAGCCGTGGATCCCAAAGGAAGAGCTGTTATGATTAGTGCGATTGAGAAGCAGAAATTG GTGTATATCTTGAACAGAGACGCTGCAGCCCGACTTACCATTTCATCTCCCCTGGAGGCCCACAAAGCAA ACACTCTAGTGTACCATGTGGTTGGAGTAGATGTGGGATTTGAAAATCCTATGTTTGCTTGTCTGGAAAT GGATTATGAGGAAGCAGACAATGATCCAACAGGGGAAGCAGCAGCCAATACCCAGCAGACACTTACTTTC TATGAATTGGACCTTGGTTTAAATCACGTGGTTCGGAAATACAGTGAACCGTTGGAGGAACATGGCAACT TCCTTATTACAGTTCCTGGAGGGTCAGATGGTCCAAGTGGAGTGCTGATCTGCTCTGAGAATTATATCAC CTACAAGAACTTTGGTGACCAGCCAGACATCCGATGTCCAATTCCCAGGAGACGGAATGACCTGGATGAC CCTGAAAGAGGAATGATTTTTGTTTGCTCTGCCACCCATAAGACCAAGTCCATGTTCTTCTTTTTGGCCC AAACGGAGCAGGGAGATATCTTTAAGATCACTTTGGAGACAGATGAAGATATGGTAACTGAGATCCGCCT CAAGTATTTTGATACTGTTCCTGTTGCTGCTGCTATGTGTGTGCTTAAAACAGGCTTCCTTTTTGTGGCA TCAGAATTTGGAAATCATTACTTGTATCAAATTGCCCATCTTGGAGATGATGATGAAGAGCCTGAGTTTT CATCTGCCATGCCTTTAGAAGAAGGAGACACATTCTTCTTTCAGCCGAGACCACTCAAAAATCTCGTGCT GGTGGATGAACTGGACAGCCTCTCTCCTATTCTCTTTTGCCAGATAGCTGATTTGGCCAATGAAGACACT CCACAGCTGTATGTAGCCTGTGGTAGGGGACCCCGGTCGTCTCTGAGAGTCTTAAGACATGGACTTGAGG TGTCGGAAATGGCTGTTTCTGAGCTCCCTGGTAACCCCAATGCTGTCTGGACTGTCCGTCGGCACATTGA AGATGAGTTTGATGCCTACATCATTGTGTCTTTCGTGAATGCCACGCTGGTGCTGTCCATCGGAGAGACT GTGGAAGAAGTGACAGACTCCGGGTTCTTGGGTACCACTCCAACCTTGTCCTGTTCCTTATTGGGAGATG ACGCGTTGGTGCAGGTGTATCCAGATGGCATTCGACACATCCGAGCAGACAAGAGAGTCAATGAGTGGAA GACTCCTGGCAAGAAGACAATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATCGCCTTGACTGGG GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGTTGAATGAGTACACGGAGCGGAAAGAAATGT CAGCAGATGTGGTGTGCATGAGTCTGGCCAACGTACCCCCTGGAGAACAGCGGTCTCGGTTCCTGGCTGT GGGGCTGGTGGACAACACTGTCAGAATCATCTCCCTTGACCCATCAGACTGTTTGCAGCCTCTGAGTATG CAGGCTCTCCCAGCCCAGCCTGAGTCTCTGTGTATTGTGGAAATGGGTGGGACCGAGAAACAAGATGAGC TGGGTGAGAGGGGCTCTATCGGCTTCCTGTACCTGAACATTGGACTCCAGAACGGTGTGCTGCTGAGGAC TGTCCTAGACCCTGTTACTGGGGACCTGTCTGACACCCGCACTAGGTATCTGGGGTCCCGTCCTGTGAAG CTCTTTCGGGTTCGGATGCAAGGCCAGGAGGCTGTTCTGGCTATGTCTAGTCGCTCATGGCTGAGCTACT CTTACCAGTCTCGATTCCATCTCACCCCTCTGTCTTATGAGACCCTGGAATTTGCATCGGGCTTTGCCTC TGAACAGTGTCCTGAAGGCATCGTTGCCATCTCCACCAACACTTTGAGGATTTTGGCACTAGAGAAGCTT GGCGCTGTCTTCAATCAAGTGGCCTTTCCTTTGCAGTACACACCCAGGAAGTTTGTCATCCACCCTGAGA GTAACAACCTTATCATCATAGAGACGGATCACAATGCCTACACTGAGGCTACAAAAGCTCAGAGAAAGCA GCAGATGGCTGAGGAAATGGTAGAAGCAGCAGGGGAGGATGAACGGGAGCTGGCTGCAGAGATGGCAGCC GCGTTCCTCAATGAAAACCTCCCAGAATCCATTTTCGGAGCTCCCAAGGCTGGCAATGGGCAGTGGGCCT CTGTGATTCGGGTAATGAACCCTATCCAGGGGAATACACTAGACCTTGTCCAGCTGGAGCAGAATGAGGC AGCCTTTAGTGTGGCAGTGTGTAGGTTCTCCAACACTGGTGAAGACTGGTATGTGCTGGTTGGTGTTGCT AAGGACTTGATCCTGAGCCCTCGGTCTGTGGCAGGGGGCTTTGTGTATACATACAAGCTTGTGAACAATG GGGAAAAGTTGGAGTTTTTGCACAAGACTCCAGTGGAAGAAGTCCCTGCTGCCATTGCCCCATTCCAGGG GAGGGTACTGATTGGTGTGGGGAAACTTTTACGAGTCTATGACCTGGGGAAGAAGAAGTTACTTCGAAAG TGTGAGAATAAGCATATTGCCAACTACATATCAGGGATCCAGACAATTGGTCACAGGGTAATCGTGTCTG ATGTCCAGGAAAGTTTCATCTGGGTTCGCTACAAGCGTAATGAGAACCAGCTCATTATCTTTGCTGATGA CACCTATCCCAGATGGGTCACCACTGCCAGCCTTCTCGACTATGATACTGTGGCTGGGGCAGACAAGTTT GGCAACATTTGTGTGGTGAGGCTCCCACCTAACACCAACGATGAGGTGGATGAAGATCCCACTGGAAACA AAGCCCTGTGGGACCGGGGCTTGCTCAATGGGGCTTCACAGAAGGCAGAGGTCATCATGAACTACCACGT TGGGGAGACAGTGCTGTCGTTGCAGAAGACCACACTGATCCCTGGAGGCTCAGAATCACTTGTCTATACC ACTTTATCTGGAGGAATTGGCATCCTTGTCCCTTTCACGTCTCATGAGGACCACGACTTTTTCCAGCATG TTGAGATGCACCTGCGGTCTGAGCATCCTCCTCTCTGTGGACGGGACCACCTCAGCTTTCGTTCTTATTA TTTCCCTGTTAAGAATGTGATTGATGGAGACCTTTGTGAGCAGTTCAACTCCATGGAGCCCAACAAGCAG AAAAATGTCTCTGAAGAGCTGGACCGAACCCCACCTGAAGTGTCCAAGAAGCTTGAGGATATTCGGACCC GCTATGCTTTCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001106187
Insert Size	3654 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106187.2, NP_001099657.2
RefSeq Size	4524 bp
RefSeq ORF	3654 bp
Locus ID	292019

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