Wdr77 (NM_001008771) Rat Untagged Clone
CAT#: RN203500
Wdr77 (untagged ORF) - Rat WD repeat domain 77 (Wdr77), (10 ug)
CNY 3,990.00
Product images
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Ac2-269; MEP-50; RGD1310479 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN203500 representing NM_001008771
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAAGGAAAGCCCACCTCCGCTCGTGCCCCCGGCGGCCCGCGAGTGGAACCTGCCCCCCAATGCGC CCGCCTGCATGGAACGGCAGTTGGAAGCTGCACGGTACCGCTCTGATGGTTCCCTTCTGCTCGGGGCTTC CAGTCTGAGTGGTCGCTGCTGGGCAGGATCTCTGTGGTTTTTCAAGGATCCTAGTGCGGCCCCCAACGAA GGTTTCTGCTCTGCTGGCGTCCAGACGGAGGCTGGAGTAGCTGACCTCACTTGGGTTGGGGACAAAGGTA TCTTAGTGGCTTCTGATTCAGGTGCTGTTGAATTGTGGGAGCTAGATGAGAACGAGACACTTATTGTCAG CAAGTTCTGCAAGTATGAGCATGATGACATTGTGTCTACTGTCACTGTCCTGAGCTCCGGCACACAGGCT GTCAGTGGTAGCAAAGACTTCTGCATCAAAATTTGGGACCTGGCTCAGCAGATGTCGCTGAATTCATACC GAGCTCATGCGGGACAGGTCACCTGTGTTGCTGCCTCTCCCCACAGAGAAACTGTGTTTCTTTCATGCAG TGAGGACAGTAGAATTTTGCTCTGGGATACCCGCTGTCCCAAGCCGGCATCACAGATGGGCTGCAATGCC TCTGGCTACCTCCCTACCTCCCTGGCTTGGCATCCTCAGCAGAGTGAAATCTTTGTCTTTGGTGATGAGA ATGGATCTGTCTCCCTTGTGGACACCAAGAATGCAAGCTGCACCCTCAGCTCAGCTGTGCACTCGCAGTG TGTCACTAGACTGGTGTTCTCTCCACACAGTGTTCCCTTCCTGGCCTCTCTCAGTGAAGACTGTTCACTT GCTGTGCTGGATTCAAGCTTTTCTGAGGTGTTTAGAAGTCGAGCCCACAGAGACTTTGTGAGAGATGCTA CATGGTCTCCACTCAATCACTCCCTTCTTACCACAGTTGGCTGGGACCATCAGGTCATCCACCATGTTGT GCCGTTAGAGCCTCTCCCAGCCCCTGGACCTGACAGTGTTGTGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001008771 |
Insert Size | 1029 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001008771.3, NP_001008771.2 |
RefSeq Size | 2047 bp |
RefSeq ORF | 1029 bp |
Locus ID | 310769 |
UniProt ID | Q4QR85 |
Gene Summary | Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR203500 | Wdr77 (Myc-DDK-tagged ORF) - Rat WD repeat domain 77 (Wdr77), (10 ug) |
CNY 3,990.00 |
|
RR203500L3 | Lenti ORF clone of Wdr77 (Myc-DDK-tagged ORF) - Rat WD repeat domain 77 (Wdr77), (10 ug) |
CNY 6,080.00 |
|
RR203500L4 | Lenti ORF clone of Wdr77 (mGFP-tagged ORF) - Rat WD repeat domain 77 (Wdr77), (10 ug) |
CNY 6,650.00 |