Myh1 (NM_001135158) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MYHC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203442 representing NM_001135158 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCGACGCCGAGATGGCCGTTTTCGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGC GAATCGAGGCTCAGAACAAGCCTTTTGATGCCAAGTCATCCGTGTTTGTGGTGGATGCTAAGGAGTCTTT TGTAAAAGCAACGGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGGTGGCGCTACTGTA ACAGTAAAAGATGATCAAGTCTTCCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA TGACCCACCTCCACGAGCCTGCTGTGCTGTACAACCTCAAAGAGCGTTACGCAGCCTGGATGATCTACAC CTACTCGGGCCTGTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCAGTGTATAATGCAGAGGTGGTG GCAGCTTACCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCTATCTCTGACAACGCCTACC AGTTCATGTTGACAGATCGGGAGAACCAGTCTATTTTGATCACCGGAGAATCTGGGGCTGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGAGAAGAAGAAGGAGGAAGCA CCTTCTGGCAAAATGCAGGGGACCCTGGAGGATCAAATCATCAGTGCCAATCCTCTGCTGGAAGCCTTCG GGAATGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATCCACTTTGGTAC CACAGGGAAGCTGGCTTCTGCTGATATTGAAACATATCTTCTGGAGAAGTCTAGAGTCACCTTCCAGCTA AAAGCTGAGAGAAGCTACCATATTTTTTACCAGATCATGTCTAACAAGAAGCCAGATCTAATTGAAATGC TCCTGATCACCACCAACCCATACGACTACGCCTTTGTCAGTCAGGGGGAGATCACAGTGCCCAGCATTGA TGACCAAGAAGAGTTGATGGCCACAGATAGTGCCATTGATATTCTGGGCTTCACTTCTGATGAAAGAGTC TCCATCTATAAGCTCACGGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAACGTGAGG AGCAGGCTGAGCCGGATGGCACGGAAGTGGCTGACAAGGCTGCCTATCTCCAAAATCTGAACTCTGCTGA CCTGCTCAAAGCCCTGTGTTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACTGTG CAGCAGGTGTACAACGCTGTGGGTGCCCTGGCCAAAGCTGTCTACGAGAAGATGTTCCTTTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCA TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCAATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCCAAAGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAACAGCATCTT GGAAAGTCCAACAACTTCCAGAAGCCCAAACCCGCCAAAGGCAAGGTTGAGGCCCACTTCTCTCTGGTGC ACTATGCCGGCACCGTTGACTACAACATCGCTGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGAGAC CGTGGTGGGGCTGTACCAGAAGTCTTCAATGAAAACTCTGGCTTACCTCTTCTCTGGGGCAGCAGCTGCT GCTGAAGCAGAGTCTGGTGGCGGTGGTGGAAAGAAAGGGGCAAAGAAGAAGGGTTCTTCCTTCCAGACTG TGTCAGCTCTCTTCAGGGAGAACCTGAATAAGCTGATGACCAATCTGAGGAGCACTCACCCCCACTTTGT CCGGTGCATCATCCCCAATGAGACTAAGACGCCTGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTG AGGTGTAACGGGGTACTGGAAGGCATCCGCATCTGCAGGAAGGGGTTCCCCAGCAGGATCCTCTACGCAG ACTTCAAGCAGAGATACAAGGTGTTAAATGCAAGTGCCATCCCCGAGGGACAATTCATCGATAGCAAGAA GGCTTCTGAGAAGCTCCTTGGCTCCATCGACATTGACCACACTCAGTATAAGTTTGGTCACACCAAGGTC TTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTGATTACCC GGACGCAGGCCATGTGCCGAGGGTACTTGGCAAGAGTGGAGTACCAGAAGATGGTGGAGCGAAGAGAGTC CATCTTCTGCATCCAGTACAACGTCCGAGCCTTCATGAATGTGAAACACTGGCCCTGGATGAAGCTGTAT TTCAAGATCAAACCTCTCTTGAAGAGTGCCGAGACAGAGAAGGAGATGGCCAACATGAAGGAAGAGTTTG AAAAAACTAAAGAAAACCTTGCAAAGGCAGAGGCCAAAAGGAAAGAGCTAGAAGAAAAGATGGTGGCTCT GATGCAGGAGAAAAATGACCTGCAGCTCCAAGTTCAGTCTGAAGCAGACAGCTTGGCTGATGCTGAGGAA AGATGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACTGAGAGAGCAG AGGATGAGGAAGAGATCAACGCCGAGCTCACCGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCT GAAGAAAGACATCGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAAC AAGGTGAAAAACCTCACAGAGGAGATGGCGGGCCTGGACGAAACCATCGCCAAGCTGACCAAGGAGAAGA AGGCCTTGCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCT GACCAAAGCCAAAATCAAGCTGGAACAGCAAGTGGATGACCTTGAAGGCTCTCTGGAACAAGAAAAGAAA ATCCGGATGGATCTTGAAAGAGCCAAGAGGAAACTCGAGGGGGACCTAAAACTGGCCCAAGAGTCCACAA TGGACGTAGAAAATGACAAACAGCAGCTCGATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAACCT GCAGAGCAAGATCGAGGATGAACAGGCCCTCGGCATGCAGCTGCAGAAGAAGATCAAGGAGTTGCAGGCT CGCATTGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCT CTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGCGGCTGGAAGAAGCCGGCGGGGCCACTTCAGCCCA GATCGAGATGAACAAGAAGAGAGAGGCCGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTG CAGCACGAAGCCACAGCAGCCACCCTGAGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGA TCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGACGA CCTCGCTAGCAACATGGAGGTCATTTCCAAATCCAAGGGAAACCTTGAGAAGATGTGCCGCACTCTGGAG GACCAGGTGAGCGAGCTGAAGACCAAGGAGGAGGAGCAGCAGCGGCTCATCAACGAGCTGACCGCGCAGA GGGGGCGTCTGCAGACAGAGTCAGGTGAATATTCACGCCAGCTGGATGAAAAGGATTCATTAGTTTCTCA GCTCTCCAGGGGCAAACAGGCATTCACTCAACAGATTGAGGAGCTGAAGAGGCAGCTTGAAGAGGAAGTA AAGGCCAAGAGTGCCCTGGCTCATGCTCTTCAGTCCTCCCGCCATGACTGTGACCTTCTGCGGGAACAGT ATGAAGAAGAACAGGAAGCCAAGGCTGAACTGCAGAGGGCCATGTCCAAGGCCAACAGTGAAGTGGCCCA GTGGAGGACGAAATATGAGACGGATGCCATCCAGCGCACGGAAGAACTGGAGGAGGCCAAGAAGAAGCTG GCTCAGCGTCTGCAGGATGCTGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGTGCTTCCCTGGAGAAGA CGAAGCAGAGGCTGCAGAATGAGGTGGAGGACCTCATGATTGATGTGGAGAGGACCAACGCTGCCTGTGC CGCCCTGGACAAGAAGCAGAGAAACTTCGACAAGATCCTGGCAGAATGGAAACAGAAGTATGAGGAAACG CATGCTGAACTTGAAGCCTCCCAGAAGGAGTCCCGCTCTCTCAGCACAGAGTTGTTCAAGATTAAGAATG CTTATGAGGAATCTTTAGACCAACTTGAAACCTTGAAAAGGGAAAATAAGAATTTGCAACAGGAGATCTC TGACCTCACTGAACAGATTGCAGAAGGAGGGAAACGTATCCATGAACTGGAAAAAATAAAGAAGCAAATT GAGCAAGAAAAATCCGAGCTCCAGGCTGCTTTAGAGGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAA AGATCCTGCGCATCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGA TGAGGAAATTGACCAGCTGAAGAGAAACCACATTCGAGTCGTGGAGTCCATGCAGAGCACGCTGGATGCC GAGATCAGGAGCAGGAATGACGCCATCAGGATCAAGAAGAAGATGGAGGGAGACCTCAATGAGATGGAAA TCCAGCTGAACCACTCCAACCGCATGGCTGCTGAGGCCCTGAGAAACTACAGGAACACCCAAGGCATCCT CAAGGACACCCAGTTGCACCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATG GTGGAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAGCAGACTGAGA GGAGCAGGAAGATTGCAGAGCAGGAACTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAA CACCAGCCTCATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAGATCCAGGGAGAGATGGAGGAC ATCGTCCAGGAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAAGCCATCACTGACGCCGCCATGATGGCGG AGGAGCTGAAGAAGGAGCAGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGT GAAGGACCTGCAGCACCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAG AAACTGGAGGCCAGGGTCCGTGAACTTGAAGGAGAAGTTGAAAACGAACAGAAGCGCAATGTTGAAGCTA TCAAGGGTCTGCGCAAACATGAGAGAAGAGTGAAAGAACTCACTTACCAAACTGAGGAAGACCGCAAGAA CGTTCTCAGGCTCCAGGACCTTGTGGACAAACTGCAATCAAAGGTCAAAGCATACAAGAGACAAGCCGAG GAAGCGGAGGAACAATCCAACGTCAACCTGGCCAAGTTCCGCAAGATCCAGCACGAGCTGGAGGAAGCCG AGGAGCGGGCTGACATCGCCGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGCGAGGTTCACACCAA AATCATAAGCGAAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135158
Insert Size	5829 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135158.1, NP_001128630.1
RefSeq Size	6031 bp
RefSeq ORF	5829 bp
Locus ID	287408
Gene Summary	one of three isoforms of myosin heavy chain; components of fast-twitch fibers in skeletal muscle; responsible for myosin ATPase activity and control of fiber shortening speed and resistance to fatigue [RGD, Feb 2006]

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