Cckar (NM_012688) Rat Untagged Clone

CAT#: RN203242

Cckar (untagged ORF) - Rat cholecystokinin A receptor (Cckar), (10 ug)



  "NM_012688" in other vectors (3)

CNY 3,656.00

CNY 5,700.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Cck-ar
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN203242 representing NM_012688
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCATTCACCAGCTCGCCAGCACTTGGTAGAAAGCAGCAGGATGGACGTGGTCGACAGCCTTCTTA
TGAATGGGAGCAACATCACTCCCCCCTGTGAACTCGGACTGGAAAATGAGACGCTTTTCTGCTTGGATCA
ACCTCAACCTTCAAAAGAGTGGCAGTCTGCACTGCAGATTCTCCTGTACTCCATCATATTCCTTCTCAGT
GTGCTGGGGAACACGCTGGTTATAACGGTGCTGATTCGAAACAAGAGGATGCGGACGGTCACCAACATCT
TCCTGCTGTCCCTGGCTGTCAGTGACCTCATGCTCTGCCTCTTCTGCATGCCGTTCAACCTCATCCCCAA
CCTGCTCAAGGATTTCATCTTCGGAAGTGCCGTGTGCAAGACTACCACCTACTTCATGGGCACTTCCGTG
AGCGTTTCCACCTTCAACCTGGTAGCCATCTCTCTGGAGAGATATGGCGCCATCTGCAGACCCCTACAGT
CCCGCGTCTGGCAAACAAAGTCCCATGCTTTGAAGGTCATCGCTGCCACCTGGTGCCTCTCCTTTACCAT
CATGACTCCGTACCCCATTTACAGCAACTTGGTGCCTTTTACTAAAAATAATAACCAGACGGCGAACATG
TGCCGCTTCCTGTTGCCAAGTGACGCTATGCAGCAGTCCTGGCAAACATTCCTGCTACTCATCCTCTTTC
TTCTCCCTGGGATTGTGATGGTGGTGGCCTACGGGTTGATCTCTCTGGAACTCTACCAAGGAATCAAATT
TGATGCCAGCCAGAAGAAATCTGCCAAAGAGAAGAAGCCGAGCACTGGCAGCAGCACCCGATATGAGGAT
AGTGATGGCTGTTACTTGCAGAAGTCCCGGCCCCCGAGGAAGCTGGAGCTTCAGCAGCTGTCTAGCGGCA
GCGGTGGCAGCAGACTCAACCGGATCAGGAGCAGCAGTTCAGCTGCCAACCTGATAGCCAAGAAGCGCGT
GATCCGCATGCTCATTGTCATCGTGGTCCTCTTCTTCCTGTGCTGGATGCCCATCTTCAGCGCCAACGCC
TGGCGGGCATATGACACGGTTTCTGCCGAGAAGCACCTCTCAGGGACTCCCATCTCCTTCATCCTCCTCC
TCTCCTACACCTCCTCCTGTGTTAACCCCATCATCTATTGCTTCATGAACAAACGCTTTCGCCTGGGCTT
CATGGCCACCTTCCCTTGTTGCCCGAATCCCGGTCCCCCAGGGGTGAGAGGAGAGGTGGGAGAGGAGGAG
GATGGGAGGACCATAAGGGCATTGCTGTCCAGGTATTCCTACAGCCACATGAGCACCTCTGCTCCACCCC
CCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_012688
Insert Size 1335 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012688.2, NP_036820.1
RefSeq Size 1502 bp
RefSeq ORF 1335 bp
Locus ID 24889
UniProt ID P30551
Gene Summary G-protein coupled receptor activated by cholecystokinin; may be involved in food intake and insulin regulation [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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