Tpp2 (NM_031137) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	TPP-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203155 representing NM_031137 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCGCTGCGACCGAGGAGCCTTTCCCTTTCCATGGTCTTCTACCAAAGAAAGAGACTGGGGCCT CCTCCTTCCTGTGCCGCTACCCGGAGTATGACGGACGCGGGGTGCTCATCGCCGTCCTGGACACAGGGGT TGATCCCGGGGCCCCGGGCATGCAGGTCACGACTGATGGGAAACCAAAAATCATTGATATCATTGATACA ACAGGAAGTGGTGATGTGAATACTGCTACAGAAGTAGAACCAAAAGATGGCGAAATCACTGGTCTTTCTG GAAGAGTGCTTAAGATTCCTGCAAACTGGACAAATCCTTCAGGAAAATATCATATTGGCATTAAAAATGG TTATGACTTCTATCCAAAGGCTCTCAAGGAAAGGATACAGAAAGAACGGAAGGAAAAAATCTGGGATCCA ATTCACAGAGTTGCGCTTGCAGAAGCTTGTAGAAAACAAGAAGAATTTGATATTGCCAATAATGGCTCTT CCCAAGCCAATAAACTAATCAAGGAAGAGTTGCAGAGTCAAGTGGAATTACTTAATTCTTTTGAGAAAAA GTATAGTGATCCAGGCCCTGTGTATGACTGCTTGGTGTGGCATGATGGTGAGACCTGGAGAGCCTGTGTT GATTCGAATGAAAATGGGGACTTGGGTAAATCTACTGTATTGAGAAACTACAAAGAGGCTCAAGAATATG GTTCTTTTGGCACAGCTGAGATGCTGAATTACTCTGTTAATATTTATGATGATGGAAACCTGCTCTCCAT TGTGACCAGTGGAGGAGCTCATGGAACCCATGTAGCGAGTATAGCTGCTGGGCATTTTCCAGAAGAGCCT GAACGGAATGGAGTTGCTCCTGGTGCTCAAATTCTATCCATTAAGATTGGTGATACAAGGCTAAGCACCA TGGAAACAGGCACAGGCCTCATCAGAGCTATGATAGAAGTTATAAATCATAAGTGTGATCTTGTCAACTA CAGTTATGGAGAAGCAACTCATTGGCCAAATTCTGGGAGAATTTGTGAAGTAATTAATGAAGCAGTATGG AAACATAATACAATTTATGTTTCAAGTGCTGGAAATAATGGTCCATGCCTTTCTACAGTGGGTTGTCCAG GAGGAACTACTTCCAGCGTGATAGGTGTTGGAGCTTATGTTTCTCCTGATATGATGGTTGCAGAGTATTC ACTGAGAGAGAAACTACCTGCAAATCAATATACATGGTCTTCTAGAGGCCCAAGTGCTGATGGGGCCCTT GGTGTGAGCATCAGTGCACCAGGAGGTGCTATTGCTTCTGTGCCTAACTGGACATTGAGGGGGACTCAGC TAATGAATGGGACATCAATGTCTTCCCCCAATGCCTGTGGTGGCATTGCCCTGGTACTTTCAGGGCTGAA AGCAAATAATGTTGACTATACTGTTCACTCAGTCAGAAGAGCTCTAGAAAACACTGCAATAAAAGCTGAC AATATAGAAGTATTTGCCCAAGGACATGGAATTATTCAGGTTGACAAAGCTTATGACTACCTCATTCAGA ATACATCATTTGCTAACAGATTAGGTTTTACAGTTACAGTTGGAAATAACCGTGGCATCTACCTCCGAGA TCCTGTTCAGGTGGCTGCTCCTTCAGATCATGGTGTTGGCATTGAGCCTGTATTTCCAGAGAACACAGAA AACTCTGAAAAAATATCCTTCCAGCTTCATTTAGCTTTAACTTCAAATTCATCTTGGGTTCAATGTCCCA GCCATTTGGAACTCATGAATCAATGTCGGCACATAAACATACGTGTGGACCCCAGGGGCTTAAGAGAAGG GTTACATTATACAGAGGTATGTGGGTATGATATAGCATCCCCCAATGCAGGTCCTTTATTCAGAGTTCCA ATCACTGCAGTTATAGCAGCAAAAGTAAATGAATCATCACATTATGATCTAGCCTTTACAGATGTACATT TTAAACCTGGTCAGATTCGAAGACATTTTGTTGAGGTTCCTGAGGGGGCAACTTGGGCTGAAGTTACCGT GTGTTCGTGTTCTTCTGAGGTATCAGCAAAATTTGTTCTTCATGCAGTACAGCTTGTGAAGCAGAGAGCA TATCGAAGTCATGAATTTTATAAGTTTTGTTCCCTTCCAGAAAAAGGAACACTCATTGAAGCCTTTCCTG TTCTGGGTGGGAAAGCAATTGAATTTTGTATTGCTCGTTGGTGGGCAAGTCTCAGTGATGTCAATATTGA TTATACCATATCATTCCATGGCATAGTGTGTACTGCACCACAGTTAAACATTCATGCATCTGAAGGAATC AATCGTTTTGATGTCCAGTCCTCTTTAAAATATGAAGATCTGGCTCCTTGCATAACTTTGAAGAGCTGGG TGCAAACACTACGCCCAGTAAATGCAAAAACCAGACCTTTAGGATCAAGAGATGTTTTGCCAAATAATCG CCAGCTTTATGAGATGGTCCTGACATACAGCTTTCATCAGCCCAAGAGCGGAGAAGTAACACCTAGTTGT CCACTCCTTTGTGAATTATTATATGAGTCAGAATTTGACAGTCAGTTGTGGATTATTTTTGACCAGAACA AAAGACAGATGGGCTCAGGCGATGCCTATCCACATCAGTATTCTTTGAAATTGGAGAAAGGAGATTATAC GATTCGATTACAGATTCGTCATGAGCAAATCAGTGATTTGGATCGTCTCAAAGATCTTCCGTTTATTGTT TCGCATAGGTTGTCTAATACCTTGAGCTTAGATATTCATGAAAATCATAGCCTTGCACTTCTAGGAAAGA AGAAATCAAGCAGTTTGACATTACCACCCAAATATAATCAGCCATTCTTTGTTACTTCCTTACCTGATGA TAAAATACCTAAGGGGGCAGGACCCGGATGCTACCTTGCAGGCTCCTTGACATTGTCAAAGACTGAGCTT GGAAAGAAAGCTGATGTGATCCCTGTTCATTACTATCTAATACCTCCACCAACAAAGACTAAGAATGGCA GCAAAGATAAAGAAAAGGATTCAGAAAAAGAGAAAGATTTGAAAGAAGAGTTTACTGAAGCTTTACGAGA TCTTAAAATTCAGTGGATGACCAAGCTGGATTCTACTGACATTTACAATGAATTGAAAGAAACATATCCT GCTTACCTTCCTTTGTATGTTGCACGTCTTCATCAATTAGATGCTGAAAAGGAGCGGATGAAAAGACTTA ATGAAATTGTTGATGCTGCCAATGCTGTTATTTCTCACATCGATCAGACCGCTCTCGCTGTTTACATTGC CATGAAGACTGACCCCAGGCCTGATGCAGCTACTATAAAAAATGATATGGACAAGCAGAAATCTACCCTG GTAGATGCCCTCTGCAGGAAAGGATGTGCTCTGGCAGACCACCTTCTTCATGCACAGCCCCACGATGGGG CAGCAGCTGGAGATGCAGAAGCAAAAGAAGAGGAAGGAGAAAGTACCTTGGAATCTCTATCAGAAACCTA TTGGGAAACTACAAAGTGGACAGATCTTTTTGACACTAAGGTTTTGACATTTGCATACAAGCATGCATTA GTAAATAAGATGTACGGGAGAGGCCTTAAGTTTGCAACCAAACTCGTAGAAGAAAAACCAACAAAAGAAA ACTGGAAAAATTGTATTCAACTGATGAAATTACTCGGATGGACCCATTGTGCGTCTTTTACTGAAAACTG GCTCCCCATCATGTATCCTCCTGATTATTGTGTATTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031137
Insert Size	3750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031137.1, NP_112399.1
RefSeq Size	4566 bp
RefSeq ORF	3750 bp
Locus ID	81815
UniProt ID	Q64560
Gene Summary	extralysosomal enzyme involved in degrading peptides generated by the proteasome [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.