Mtr (NM_030864) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	methioninesynthase
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203085 representing NM_030864 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAAACCCTGCAGGATGAGATTGAAGCCATTCTTCGGAAGAGGATTATGGTGCTAGATGGCGGGA TGGGGACCATGATCCAGCGGTACAAACTAAGCGAAGAAAATTTCCAAGGTCAAGAGTTTAAAGACCACAG CAGGCCACTGAAAGGCAACAATGACATCTTGAGTATAACTCAGCCTGATGTTATTTACCAGATTCATAAG GAATACTTGCTGGCTGGAGCAGACATCATTGAAACAAACACTTTCAGCAGCACTAGTATCGCCCAGGCTG ACTATGGCCTCGAACACTTGGCCTACCGGATGAACAAATGCTCCGCAGACGTGGCCAGAAAAGCCGCCGA GGAGATAACTCTGCAGACAGGAGTCAAGAGGTTTGTGGCTGGATCTCTGGGTCCGACTAACAAGACACTT TCAGTTTCCCCATCTGTGGAAAGGCCAGATTACAGGAACATCACATTTGATGAGCTTGTTGAAGCATACC AGGAGCAGGCCAAGGGACTGCTGGATGGTGGGGTTGACATCTTACTCATTGAGACGATTTTTGATACAGC CAATGCCAAAGCAGCCTTGTTTGCGCTCCAGAAACTCTTTGAAGAGAATTATGCTTCTCCCAGGCCTATC TTTATTTCTGGGACCATTGTTGATAAAAGTGGACGGACTCTTTCTGGCCAGACAGGAGAGGCTTTTGTCA CCAGCGTGTCTCATTCAGACCCCCTGTGCATTGGATTAAATTGTGCTCTGGGTGCAGCTGAAATGAGGCC TTTCATTGAAACAATTGGAAAATGTACAACAGCTTATGTCCTCTGTTACCCAAATGCAGGTCTTCCCAAT ACCTTTGGTGATTATGATGAAACACCAGCCATGATGGCCATGCACCTGAAGGACTTTGCCGTGGACGGCT TGGTGAATGTAGTTGGTGGATGCTGTGGTTCAACGCCTGATCACATCAGGGAAATTGCAGAAGCTGTGAA AAATTGTAAGCCAAGAGTTCCCCCTGATAGTGTTTTTGAAGGACATATGTTACTGTCTGGTCTAGAGCCC TTCAGGATTGGACCATACACTAACTTTGTTAACATTGGAGAGCGCTGTAACGTGGCAGGATCCAAGAAAT TTGCTAAGCTCATCATGGCAGGAAACTATGAAGAAGCCCTGAGCGTTGCCAAAGTGCAGGTGGAAATGGG AGCTCAGGTGCTGGATATCAACATGGATGACGGCATGCTGGATGGTCCCAGTGCGATGACCAAATTTTGC AACTTCATTGCTTCGGAGCCTGACATTGCCAAGGTGCCCCTGTGCATTGACTCTTCCAATTTTGCTGTGA TCGAGGCGGGGTTGAAGTGCTGCCAAGGGAAGTGCATAGTCAACAGCATCAGCCTGAAGGAGGGGGAGGA GGACTTCCTGGAGAAGGCCAGGAAGATTAAGAAGTTTGGAGCTGCTGTGGTGGTTATGGCTTTTGATGAG GAAGGACAGGCAACAGAAACAGATGTTAAAGTCAGTGTGTGCACTAGAGCCTACCATCTACTTGTGGAAA AAGTGGGCTTCAATCCAAATGATATCATCTTTGACCCCAACATCCTTACCATTGGAACTGGAATGGAAGA ACATAACTTGTATGCTATCAATTTTATTCATGCAACGAGAGTCATTAAGGAAACATTACCAGGAGTCAGA ATAAGTGGAGGTCTCTCCAACCTATCCTTCGCCTTCCGAGGGATGGACGCCATTCGAGAAGCAATGCACG GTGTTTTCCTTTATCATGCAATCAAGTTCGGTATGGACATGGGGATAGTCAATGCCGGCAGTCTCCCCGT GTACGATGATATCCATAAGGACCTTCTTCAGCTCTGTGAAGACCTCATCTGGAACAGAGACGCTGAGGCT ACTGAGAAACTCTTACGATATGCCCAGACTCATGGCAAAGGAGGGAAGAAAGTCATCCAGACAGATGAGT GGAGGAATGGCTCGATTGAAGAGCGCCTGGAGTATGCTCTTGTGAAGGGCATTGAAAAGCACATTGTTGA AGACACTGAAGAAGCCAGGTTAAACCGGGAAAAATACCCCCGGCCTCTGAATATAATTGAAGGGCCCCTG ATGAATGGCATGAAAGTGGTTGGTGATCTTTTTGGAGCTGGAAAAATGTTTCTACCTCAGGTTATAAAGT CAGCCCGTGTCATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAAGCCAG GGTGCTTAATGGTTCGGTTGAAGAAGAGGACCCTTACCAAGGCACCATTGTGCTGGCCACTGTTAAAGGT GATGTGCATGACATTGGCAAGAACATTGTTGGTGTGGTGCTGGGCTGTAATAATTTCAGAGTTATTGATT TAGGAGTCATGACTCCCTGTGATAAGATACTTCAAGCTGCTCTGGACCACAAAGCAGATATAATTGGCTT GTCAGGACTTATCACGCCATCCTTGGATGAAATGATTTTTGTTGCCAAGGAAATGGAGAGGTTGGCTATA AAGATTCCATTGTTGATAGGAGGAGCAACCACTTCAAGAACCCACACAGCAGTTAAAATAGCACCACGGT ACAGTGCGCCTGTAATCCATGTTCTAGATGCATCCAAGAGTGTGGTGGTGTGTTCTCAGCTGTTAGATGA AAATCTGAAAGATGACTACTTTGAAGAAATACTGGAAGAGTATGAAGATATTAGACAGGACCACTACGAG TCTCTCAAAGAGAGAAAATACTTACCCCTAAGTCAAGCCAGAAAACACAGTTTCCACATTGATTGGCTGT CTGAACCTCACCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTTGAAGACTACAACCTGCAAAAGCT GGTGGACTACATTGACTGGAAGCCTTTCTTTGATGTCTGGCAGCTCCGCGGCAAGTACCCGAACCGAGGA TTTCCCAAGATATTCAATGACAAAGCAGTAGGTGAAGAAGCCAGAAAGGTATATGAGGATGCTCAGAACA TGCTGAGCATACTGATCAGTCGAAAGAAACTCCGGGCCAGGGGTGTGGTTGGATTCTGGCCAGCACAGAG CGTCCAAGATGACATCCACCTGTATGCAGAGGGGGCGGTGCCCCAGGCCGCCGAGCCCATAGCCACCTTC TATGGACTGAGGCAGCAGGCTGAGAAGGACTCCTCCAGTACAGACCCCTACCACTGCCTCTCAGACTTCG TTGCTCCTCTGCATTCTGGTGTCCGTGACTACTTGGGTCTGTTTGCTGTGGCCTGCTTTGGGGTTGAAGA GCTGAGTAAGGCCTATGAGGATGATGGCGATGACTACAGCAGCATCATGGTGAAGGCCCTGGGGGACAGG CTGGCGGAGGCCTTTGCAGAGGAGCTCCACGAGAGAGTGCGCAGAGAACTCTGGGCTTACTGTGGGAGTG AGCAGTTGGGTGTCACAGACTTGCGCAAACTCCGCTATGAGGGCATCCGGCCAGCTCCTGGTTACCCCAG CCAGCCTGACCATACCGAGAAGCTCACCATGTGGAGGCTGGCCAACATCGAGCAGGCCACAGGCATCAGG TTGACAGAATCCTTGGCAATGGCACCTGCTTCAGCAGTGTCGGGTCTCTACTTCTCCAATGTAAAGTCCA AATATTTTGCTGTGGGGAAAATTTCCAAAGATCAGATTGAGGATTATGCCTTGAGGAAGAACATGTCAGT GGCCGAGGTGGAAAAATGGCTTGGCCCCATTCTGGGATATGATACAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_030864
Insert Size	3762 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030864.1, NP_110491.1
RefSeq Size	4498 bp
RefSeq ORF	3762 bp
Locus ID	81522
UniProt ID	Q9Z2Q4
Gene Summary	catalyzes the cobalamin-dependent conversion of 5-methyltetrahydrofolate and L-homocysteine to tetrahydrofolate and L-methionine [RGD, Feb 2006]

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