Pard3 (NM_031235) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Par3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202843 representing NM_031235 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGACCGTGTGCTTCGGGAGGACCCGGGTGGTCGTGCCGTGCGGAGATGGCCGCATGAAAGTTT TCAGCCTTATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCGTGGCCAAGGATCCAAACTACTGGATACA GGTACATCGCTTGGAGCATGGGGACGGTGGGATTCTAGACCTGGATGACATCCTCTGTGACGTTGCCGAT GACAAAGACAGACTGGTAGCAGTGTTTGATGAGCAGGATCCCCACCATGGAGGAGATGGTACGAGTGCCA GCTCCACGGGAACCCAGAGTCCAGAGATATTCGGCAGTGAGCTGGGCACCAACAATGTTTCTGCTTTTCG GCCTTATCAAACCACAAGTGAAATTGAGGTCACGCCTTCAGTCCTTCGGGCAAATATGCCTCTTCACGTC CGACGGAGCAGTGACCCAGCTTTAACTGGCCTATCCACTTCCGTCAGTGATAACAACTTTTCCTCAGAGG AGCCCTCCAGGAAAAACCCCACCCGCTGGTCCACGACAGCTGGCTTTCTCAAGCAGAACACCACTGGAAG TCCCAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGGAGCCTCCCACGGGATCCCAGTAGCTGGTCC AACCAGTTCCAGAGAGACAACGCCCGCTCCTCTCTGAGTGCCAGCCACCCAATGGTAGACCGGTGGCTGG AGAAGCAAGAACAGGACGAGGAAGGCACAGAAGAAGACAGCAGCCGAGTGGAGCCGGTTGGACATGCTGA TACTGGACTGGAGAATATGCCCAACTTTTCCCTCGATGATATGGTAAAGCTCGTACAAGTCCCCAACGAT GGAGGGCCCCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACACTGGGGTTGTTAGTGA AGCGGTTGGAGAAAGGTGGGAAGGCTGAACAAGAAAACCTTTTCCACGAGAATGACTGCATTGTGAGGAT TAACGATGGAGATCTTCGAAACAGAAGATTTGAGCAAGCACAGCATATGTTCCGCCAAGCCATGCGTGCG CGAGTCATTTGGTTCCACGTGGTCCCTGCAGCGAACAAGGAGCAGTATGAACAGCTCTCCCAACGAGAGA TGAACAACTATTCCCCTGGCCGCTTCAGCCCCGACAGCCACTGTGTGGCCAACAGGAGTGTGGCCAACAA TGCGCCTCAGGCGTTGCCCAGAGCACCCAGACTGAGCCAGCCACCTGAGCAGCTGGATGCTCACCCCCGA CTACCTCATAGTGCTCATGCCTCAACCAAACCGCCCACAGCCCCAGCCTTGGCTCCACCGAATGTGCTTA GTACCAGTGTCGGCAGTGTGTACAACACGAAGAGAGTAGGCAAGAGGCTCAACATTCAGCTTAAAAAAGG TACAGAAGGACTGGGATTTAGCATCACTTCCCGAGACGTAACTATAGGTGGCTCAGCTCCCATTTATGTC AAGAACATTCTTCCTCGAGGGGCTGCCATTCAGGATGGCAGGCTCAAGGCGGGAGACCGGCTAATAGAGG TCAATGGAGTAGATTTAGCGGGCAAGTCCCAGGAGGAAGTTGTTTCCCTGTTGAGAAGCACCAAGATGGA GGGGACCGTGAGCCTTCTGGTCTTTCGTCAAGAAGAGGCTTTCCACCCAAGGGAAATGAATGCCGAACCC AGCCAGATGCAGAGTCCAAAAGAAACGAAAGCCGAAGACGAGGACATTGTTCTCACACCTGACGGTACCA GGGAGTTTCTGACTTTCGAAGTTCCACTGAATGACTCAGGGTCTGCAGGGCTTGGTGTCAGCGTCAAGGG GAACCGCTCCAAAGAGAACCATGCAGATTTGGGGATCTTCGTTAAGTCCATCATTAACGGGGGAGCTGCA TCTAAAGATGGGAGGCTGAGGGTAAATGACCAGCTGATAGCTGTGAACGGAGAGTCTCTGCTGGGCAAAG CCAACCAGGAAGCCATGGAGACTCTGAGGAGGTCCATGTCCACCGAGGGCAACAAGCGTGGCATGATCCA GCTCATCGTGGCGAGGCGGATCAGCAGATGTAACGAGTTGCGGTCTCCTGGGAGCCCCGCTGCACCCGAG CTGCCCATAGAAACAGAGTTGGATGACAGAGAACGCAGGATCTCACACTCCCTCTACAGTGGGATCGAGG GGCTGGATGAGTCTCCCACCAGGAATGCCGCACTCAGCAGGATAATGGGTGAGTCAGGTAAATGCCAGCT CTCCCCAACCGTGAACATGCCACATGATGACACTGTCATGATTGAAGATGACAGGCTGCCTGTGCTCCCT CCTCACCTCTCTGACCAGTCCTCCTCCAGCTCCCATGATGACGTGGGATTCATAATGACAGAAGCAGGCA CGTGGGCCAAGGCTACCATCAGTGACTCAGCCGACTGCTCATTGAGTCCAGATGTTGATCCGGTTCTCGC TTTTCAACGAGAAGGATTTGGACGCCAGAGTATGTCAGAAAAACGCACAAAGCAATTTTCAAATGCCAGT CAATTGGATTTCGTTAAAACACGAAAATCAAAAAGCATGGATTTAGGTATAGCTGACGAGACTAAACTCA ATACAGTGGATGACCAGAGAGCAGGCTCCCCCAATAGAGATGTGGGACCCTCCTTGGGTCTGAAGAAATC CAGCTCTTTAGAAAGTCTGCAGACGGCTGTTGCTGAGGTGACCCTGAATGGGAACATTCCTTTCCACCGC CCACGGCCACGAATCATCCGAGGAAGGGGCTGCAACGAGAGCTTCAGAGCCGCCATTGACAAGTCCTACG ATAAGCCCATGGTGGATGACGACGACGAAGGCATGGAGACCTTGGAAGAAGACACAGAAGAAAGTTCAAG GTCAGGGAGGGAGTCCGTGTCCACGTCCAGTGATCAGCCTTCCTATTCTCTGGAGAGACAAATGAATGGA GACCCAGAGAAGAGGGACAAGGCAGAGAAGAAAAAGGACAAAGCCGGAAAGGATAAGAAGAAAGACCGAG AGAAGGAGAAGGATAAACTGAAAGCCAAGAAGGGGATGCTGAAAGGCTTGGGGGACATGTTCAGGTTTGG CAAACATCGAAAAGATGACAAGATGGAAAAAATGGGTCGAATAAAAATCCAGGACTCTTTCACCTCAGAA GAGGACAGGGTGCGGATGAAGGAGGAACAGGAGAGGATTCAAGCCAAAACTCGAGAATTTAGGGAGCGGC AAGCTCGAGAGCGCGACTATGCTGAAATCCAAGATTTCCATCGAACATTTGGCTGTGATGACGAGTTGCT GTATGGGGGCATGTCATCCTATGATGGCTGCCTGGCTCTCAATGCCAGACCCCAGAGCCCAAGAGAAGGT CACCTGATGGACACTTTGTATGCACAAGTAAAGAAACCTCGGAGCTCCAAACCTGGGGACAGCAATCGAT CCACCCCTAGCAACCATGACCGGATACAACGTCTACGGCAAGAATTCCAGCAAGCCAAACAGGATGAGGA CGTGGAAGACCGGCGCCGTACCTACAGCTTTGAGCAATCTTGGTCCAGCTCACGGCCAGCATCGCAGAGT GGTCGGCACTCGGTGTCAGTGGAGGTTCAAGTGCAGCGGCAGCGCCAGGAGGAGCGAGAGAGCTTCCAGC AGGCCCAGCGCCAGTACAGCTCCCTGCCAAGACAAAGCAGGAAGAATGCCAGCTCTGTATCACAGGATTC CTGGGAACAGAACTACGCCCCTGGTGAAGGCTTCCAGAGTGCCAAGGAGAACCCCAGGTATTCCAGTTAC CAGGGCTCCAGGAACGGCTATCTAGGTGGCCATGGCTTTAATGCCAGGGTCATGCTGGAGACCCAGGAGC TTCTCCGACAGGAGCAGAGGCGAAAAGAGCAGCAACTGAAGAAGCAGCCACCAGCGGATGGAGTCAGAGG GCCCTTCCGGCAAGATGTACCCCCGTCTCCATCTCAGGTGGCCAGGCTGAACAGACTGCAGACACCCGAG AAAGGGCGGCCCTTCTACTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031235
Insert Size	4014 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031235.1, NP_112514.1
RefSeq Size	5500 bp
RefSeq ORF	4014 bp
Locus ID	81918
UniProt ID	Q9Z340
Gene Summary	may mediate the establishment and maintenance of epithelial cell polarity [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.