Lrp6 (NM_001107892) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202754 representing NM_001107892 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTGGACAGACTGGGGAGAAGTGCCAAAGATAGAACGTGCTGGAATGGATGGTTCAAGTCGCTTTG TTATAATAAACACTGAGATTTACTGGCCAAATGGACTGACTCTGGATTATGAGGAGCGGAAGCTTTATTG GGCAGATGCAAAACTTAATTTCATCCATAAATCAAACCTGGACGGAACAAATCGACAGGCAGTGGTTAAA GGTTCCCTTCCACATCCTTTTGCCTTGACGTTATTTGAGGACACATTGTACTGGACTGACTGGAATACAC ACTCTATTTTGGCTTGCAACAAGTATACTGGCGAGGGTCTGCGTGAAATTCATTCTAACATCTTCTCTCC CATGGATATACATGCTTTCAGCCAACAGAGGCAGCCAAATGCTACAAATCCATGTGGAATTGATAATGGT GGTTGTTCCCATTTGTGTTTGATGTCTCCAGTCAAGCCTTTTTATCAGTGTGCTTGTCCAACTGGGGTCA AGCTTCTGGAGAGTGGAAAAACCTGCAAAGATGGTGCCACTGAACTATTGCTGTTAGCCCGACGGACAGA CTTGAGGCGCATCTCTTTGGATACACCAGATTTCACCGACATTGTCCTACAGTTAGAAGACATCCGACAT GCCATTGCCATAGACTACGACCCCGTGGAAGGCTACGTCTACTGGACGGACGATGAAGTACGGGCCATCC GTCGCTCCTTCATTGATGGATCTGGCAGTCAGTTTGTGGTCACGGCCCAGATTGCCCATCCTGATGGTAT TGCTGTTGACTGGGTGGCAAGAAACCTGTACTGGACAGACACTGGCACTGATCGGATAGAAGTGACAAGG CTCAATGGGACCATGAGGAAGATCCTGATTTCAGAGGACTTAGAGGAGCCCCGGGCTATTGTGTTAGATC CCATGGCTGGGTACATGTACTGGACGGACTGGGGAGAAATCCCAAAAATCGAGCGAGCTGCTCTGGACGG ATCTGACCGAGTAGTTCTTGTTAACACTTCCCTTGGTTGGCCAAATGGCTTAGCCCTGGATTATGATGAA GGCACAATATACTGGGGAGATGCCAAAACAGACAAAATTGAGGTTATGAATACTGATGGCACTGGGAGGC GAGTGCTGGTGGAAGACAAGATCCCTCACATATTTGGGTTTACCTTGCTGGGCGACTATGTTTACTGGAC TGACTGGCAGAGGCGGAGCATCGAGAGAGTGCACAAGCGGAGTGCAGAGAGGGAGGTCATAATAGACCAG CTGCCAGACCTCATGGGACTGAAGGCCACAAGCGTTCACAGAGTCATTGGTTCTAACCCCTGTGCTGAGG AGAATGGAGGATGTAGCCATCTTTGCCTCTACAGGCCTCAGGGACTTCGATGTGCCTGTCCCATTGGCTT TGAGCTCATTAGTGACATGAAGACATGCATTGTTCCTGAGGCTTTCCTTCTGTTCTCACGGAGAGCAGAT ATAAGACGCATCTCTTTGGAAACAAACAACAACAATGTGGCCATTCCTCTCACGGGTGTCAAAGAAGCTT CTGCTTTGGATTTTGATGTCACAGACAACAGAATTTACTGGACCGATATATCCCTGAAGACTATTAGCAG AGCTTTTATGAATGGCAGTGCACTGGAACATGTGGTAGAGTTCGGCTTAGATTACCCAGAAGGCATGGCA GTGGACTGGCTTGGGAAGAACTTATACTGGGCAGACACAGGAACAAATCGCATTGAGGTGTCAAAGTTGG ATGGACAGCACCGACAGGTTTTGGTATGGAAAGACCTTGACAGTCCCCGAGCTCTGGCATTGGATCCTGC TGAAGGATTTATGTATTGGACTGAGTGGGGAGGGAAGCCTAAGATTGACAGAGCGGCTATGGATGGAAGT GAACGCACTACATTAGTTCCAAATGTGGGTCGAGCAAATGGCCTTACCATCGACTATGCTAAGAGGCGGC TTTACTGGACAGACCTGGACACTAACTTAATAGAATCTTCAAATATGCTCGGACTCAACCGTGAAGTTAT AGCAGATGACTTGCCTCATCCTTTTGGCTTAACTCAGTACCAAGATTACATCTACTGGACAGACTGGAGC CGACGCAGCATTGAACGTGCCAACAAAACCAGTGGCCAAAACCGCACCATCATCCAGGGCCATTTGGATT ATGTGATGGACATCCTAGTCTTTCACTCTTCCCGGCAGGCAGGGTGGAATGAATGTGCCTCCAGCAACGG GCACTGCTCCCACCTTTGCTTGGCTGTGCCTGTTGGAGGTTTTGTGTGTGGATGCCCTGCCCACTACTCC CTGAATGCTGACAACAGGACCTGTAGTGCTCCTACCACCTTCCTGCTCTTCAGTCAGAAGAGCGCCATCA ACCGCATGGTGATTGATGAACAACAGAGTCCTGACATCATCCTTCCTATCCACAGCCTTCGGAACGTCCG GGCCATTGATTATGACCCTTTGGACAAGCAGCTCTACTGGATTGACTCTCGACAAAACATGATACGAAAG GCACAAGAAGATGGTGGCCAGGGTTTTACTGTAGTTGTAAGCTCGGTTCCAAATCAGAACCTTGAAATAC AGCCCTATGATCTCAGCATTGATATTTATAGCCGTTACATCTATTGGACCTGTGAAGCTACCAATGTCAT TGATGTGACGAGATTAGATGGTCGATCAGTTGGAGTGGTCCTAAAAGGCGAGCAAGACAGACCTCGAGCC ATTGTGGTAAACCCTGAGAAAGGGTATATGTATTTTACCAATCTTCAGGAAAGATCTCCCAAAATTGAAC GGGCTGCCTTGGATGGAACAGAACGGGAGGTTCTCTTTTTCAGTGGCCTGAGTAAGCCCGTCGCTTTGGC TCTCGACAGCAAGCTGAGCAGGCTCTTCTGGGCTGACTCCGACCTCCGGCGAATTGAGAGCAGCGACCTC TCAGGTGCCAACAGGATCGTGCTAGAGGACTCTAATATATTGCAGCCTGTGGGTCTGACCGTGTTTGAGA ACTGGCTCTACTGGATTGATAAGCAGCAACAGATGATTGAAAAAATTGACATGACTGGTCGCGAAGGCAG AACCAAGGTCCAGGCTCGGATTGCTCAGCTCAGTGACATCCATGCAGTGAAGGAGCTGAACCTTCAGGAA TACAGACAGCACCCGTGTGCCCAGGATAATGGTGGCTGTTCACATATTTGCCTTGTAAAAGGAGATGGTA CCACAAGATGCTCCTGTCCCATGCATTTAGTTCTGCTTCAGGATGAGCTGTCCTGTGGAGAGCCTCCAAC ATGTTCTCCTCAGCAGTTTACGTGTTTCACTGGGGACATTGACTGCATCCCTGTGGCCTGGCGGTGTGAT GGGTTTACTGAATGTGAAGACCACAGTGATGAGCTCAACTGTCCTGTGTGCTCGGAGTCTCAGTTCCAGT GTGCCAGCGGGCAGTGCATTGACGGCGCCCTTCGATGCAATGGTGACGCGAACTGCCAGGATAAATCGGA TGAGAAGAACTGTGAAGTGCTTTGTCTAATTGATCAGTTCCGCTGTGCCAATGGTCAGTGCGTTGGAAAA CACAAGAAATGCGACCACAATGTGGACTGCAGTGACAGATCTGATGAACTGGACTGCTATTTTGAACCCA CCACCATCCTACAGGCCGTACAGCTACCGGCACTTTGCACCGCCCACTACACCCTGCAGCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107892
Insert Size	3705 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107892.1, NP_001101362.1
RefSeq Size	5092 bp
RefSeq ORF	3705 bp
Locus ID	312781

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