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Lgr5 (NM_001106784) Rat Untagged Clone
CAT＃: RN202738
Lgr5 (untagged ORF) - Rat leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10 ug)
CNY 9,976.00
货期*
现货
规格
Product images
经常一起买 (2)
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gpr49 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN202738 representing NM_001106784
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACCTCCCGCGTCCGCATGCTCCTGTCCTTGCTGGCGCTGCTGCAGCTGGTGGCTGCCGGCAGCC CACCGAGACCGGACACGATGCCGCGGGGCTGCCCATCATACTGTCACTGTGAGCTGGATGGCAGGATGCT GCTCAGGGTGGACTGCTCCGACCTGGGGCTCTCGGAGCTGCCCTCCAACCTCAGCGTCTTCACCTCCTAC CTGGACCTCAGTATGAACAACATCAGTCAGCTACCTGCCAGTCTCCTGCATCGCCTCCGCTTCCTAGAAG AGTTGCGTCTTGCTGGAAATGCTTTGACACACATTCCCAAGGGAGCATTCGCAGGCCTTCACAGTCTCAA AGTTCTCATGCTGCAGAATAACCAGCTGAGACAGGTTCCGGAGGAAGCGCTACAGAACCTGAGAAGCCTT CAGTCCCTGCGCTTGGATGCCAACCACATCAGCTACGTGCCACCCAGCTGTTTCAGTGGTCTGCACTCCC TGAGGCACCTGTGGCTAGATGACAACGCTCTCACAGATGTCCCTGTCCAGGCTTTCAGAAGTTTATCAGC CCTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCACATAGCAGACCATGCCTTTGGAAACCTCTCC AGCCTCGTGGTTCTGCATCTCCATAATAATAGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGACTCC ACAGCCTGGAGACTTTAGATTTAAATTATAATAACCTTGACGAATTCCCCACTGCAATCAAGACACTCTC CAACCTCAAGGAACTAGGATTCCACAGCAACAATATCAGATCAATACCAGAGCGAGCATTCGTAGGCAAC CCTTCTCTTATCACAATACATTTCTATGACAATCCCATCCAGTTTGTTGGAATCTCTGCTTTTCAACACT TGCCTGAACTAAGAACGCTGACTTTGAATGGTGCCTCGCAAATTACTGAATTCCCTGACTTAACGGGGAC CGCAACCCTGGAGAGCCTGACTTTAACTGGAGCAAAGATCTCGTCTCTTCCCCAAACCGTCTGTGATCAG TTACCTAATCTCCAAGTGCTAGATTTGTCTTACAACCTGCTCGAAGACTTACCCAGTTTGTCGGGCTGCC AAAAGCTTCAGAAAATTGACCTGCGGCATAATGAAATCTATGAAATTAAAGGTGGCACTTTTCAGCAGTT GTTCAACCTCCGATCTCTGAACTTAGCCCGGAATAAAATTGCCATTATTCACCCCAACGCTTTCTCTACG TTGCCGTCTCTAATAAAGCTGGACCTATCATCCAACCTCCTTTCGTCTTTCCCTGTGACTGGGTTACACG GTTTAACTCACTTAAAATTAACGGGGAACCGCGCCTTGCAGAGCCTGATACCATCTGCAAACTTCCCGGA GCTCAAGATTATAGAAATGCCATACGCTTACCAGTGCTGTGCATTTGGGGGGTGTGAGAATGTCTATAAA ATTCCTAATCAATGGAACAAAGATGACAGCAGCAGTGTGGACGACCTTCGTAAGAAAGATGCTGGCTTAT TTCAAGTTCAAGATGAGCGGGACCTTGAAGACTTCCTACTTGACTTTGAGGAAGACCTGAAGGTTCTTCA CTCCGTGCAGTGCTCACCTCCCCCAGGTCCCTTCAAGCCCTGTGAGCACCTGTTTGGTAGCTGGCTGATC CGAATCGGGGTGTGGACCACAGCAGTCCTGGCGCTTTCCTGCAATGCCTTGGTGGCTTTCACAGTGTTCA GAACCCCTCTGTACATCTCTTCCATAAAACTGCTAATCGGGGTAATTGCGGTAGTGGACATACTCATGGG GGTCTCCAGTGCAATTCTGGCTGTCGTGGACACGTTCACTTTTGGCAGTTTTGCTCAACACGGTGCATGG TGGGAAGGTGGAATTGGCTGCCAAATTGTTGGTTTTTTGTCCATTTTTGCTTCCGAATCATCGGTCTTCC TGCTCACTCTGGCGGCACTGGAACGTGGTTTTTCTGTCAAGTGCTCTTCAAAGTTTGAAATGAAAGCTCC CCTTTCTAGCCTGAAAGCGATCATTTTGCTGTGTGTCCTCTTGGCCCTGACCATTGCCACCGTCCCCTTG CTAGGAGGCAGTGAGTACAATGCCTCTCCCCTCTGCCTGCCCTTACCCTTTGGGGAGCCCAGCACCACAG GCTACATGGTGGCTCTCGTATTGCTCAACTCCCTCTGTTTCCTCATAATGACCATTGCCTATACAAGGCT CTACTGCAGTTTGGAGAAGGGAGAGCTGGAGAACCTTTGGGACTGTTCTATGGTGAAGCACACTGCTCTG TTGCTCTTCACCAACTGTATCCTTTACTGCCCCGTGGCTTTCTTATCCTTCTCCTCTTTGCTAAACCTCA CGTTTATCAGTCCCGAAGTAATTAAATTTATACTTCTAGTGATTGTCCCACTTCCGGCATGCCTCAACCC CCTTCTCTACATTGTCTTCAACCCCCATTTTAAGGAGGATATGGGCAGCCTGGGAAAGCAAACCCGTTTC TGGACAAGAGCAAAACACCCAAGTCTGCTGTCCATTAACTCGGACGATGTTGAGAAACGGTCCTGTGACT CAACCCAAGCCTTAGTATCCTTTACCCACGCCAGCATAGCCTATGACTTGCCTTCCGATTCCGGGTCATC ACCAGCTTACCCAATGACTGAAAGCTGTCACCTCTCTTCAGTTGCATTTGTCCCATGTCTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001106784 |
| Insert Size | 2724 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001106784.1, NP_001100254.1 |
| RefSeq Size | 3130 bp |
| RefSeq ORF | 2724 bp |
| Locus ID | 299802 |
| UniProt ID | D4AC13 |
| Gene Summary | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR202738 | Lgr5 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10 ug) |
CNY 9,960.00 |
|
| RR202738L3 | Lenti ORF clone of Lgr5 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10 ug) |
CNY 13,400.00 |
|
| RR202738L4 | Lenti ORF clone of Lgr5 (mGFP-tagged ORF) - Rat leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10 ug) |
CNY 14,820.00 |
Customer
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