Smc1b (NM_001130498) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Smc1l2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202488 representing NM_001130498 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCACCTTGAGTTGCTGCTCGTGGAGAATTTCAAGTCGTGGCGAGGCCGCCAGGTCATCGGCCCTT TCAAGAGGTTCACCTGTATCATCGGCCCCAACGGCTCGGGAAAGTCTAATGTAATGGATGCACTTAGTTT TGTAATGGGAGAAAAAACAACTAATTTAAGAGTAAAAAACATTCAAGAACTTATTCATGGAGCACACATT GGAAAACCTGTTTCTTCTTCTGCAAGTGTGACCATTATATACGTAGAAGACAGTGGAGAAGAGAAAACAT TCACAAGGATTATCCGAGGGGGGTGCTCAGAATTTCATTTTGGGGATAAACCTGTGAGTCGTTCTGTGTA TGTAGCCCAGTTGGAAAACATAGGCATAATAGTCAAAGCACAGAACTGTCTAGTTTTTCAGGGAACTGTA GAGTCAATATCCATGAAAAAACCCAAAGAGAGAACCCAATTTTTTGAAGAAATCAGTACTTCAGGAGAGT TTATAGGAGAATATGAGGCAAAGAAAAAGAAGTTACAAAAAGCCGAAGAAGATGCACAATTTCATTTTAA CAGGAAAAAAAATGTAGCTGCAGAACGGAAGCATGCAAAAATAGAGAAAGAAGAGGCAGAACATTATCAA AGTCTCCTTGAAGAATTGAAAACAAACAAGATACAACTGATGCTTTTCCAACTATATTATAATGAGGAAA AAATCAATGTTTTAAACACTGAATTAGAGCATATGGACAGGAATTTAAGTGTTGTAAAAGACACTCTTTC TCACCATGAAAACATAATTAAAGCTAAGAAAAAGGATTATGGAATGTTAACTAGACAACTACAGCAAACA GCAAAAGAACTGAAATCTGTCGAAGCAGTTTTAAATCAGAAGAGGCCTCAGTACATTAAAGCTAAAGAAA ACACTTCTCACCATCTAAAAAAATTGGATTTGTCTAAGAAATTGATAACAGACAATGAAAAACAATGTGC TAAGCAGGAAGATGGCATTCGAGCCTTAGTGGCAGAACTGGTTGATTTAGATAGAGCATGGAGAAGTTTT GAAAAGCAGATGGAAGAGAAAATCTTACACAAAGGAAGAGACATTGAATTGGAAAATAGTCAGCTGGACC GTTATAAAGAGCTTAAGGAACAAGTCAGACGGAGGGTAGGTATAATGACACAACAACTGGAAAAACTACA GTGGGAACAGAAGGCAGAAAAAGAAAGACTGGCATTTGAAAGGAGGAGGCATGGAGACACTCAGGGAAAT CTAAAACAAATAAAAGAACAAATAGAAGAGCATAAAAAACGAATAGAGAAATTGGAGGAATATACAAAGA CGTGCATGGATTGCTTGGAAGATAAAAAACAGCAAGAAGAAGCCCTGATAAAAGAAATTGACAATACAAA ATCAAGAATGTCTGAAGTTAATGAAGAATTGAGTCTTATTAGAAATGAATTGCAGAATGCTGGAATTGAT AACCATGAAGGAAAACGTCAGCGGAAAAGAGCAGAAGTTCTCGAACACCTTAAAAGACTTTACCCAGATT CTGTGTTTGGAAGACTTCTTGATCTATGTCATCCTATTCATAAGAAATACCAGCTGGCTGTGACTAAGCT TTTTGGCCGGTACATGGTTGCCATTGTTGTTGCCTCAGAAAAGGTAGCAAAAGATTGTATTCGGTTTCTG AAGGAGGAAAGAGCTGAACCTGAGACATTCCTTGCTCTAGATTACCTTGATATCAAGCCAATCAATGAAC GACTAAGGGAAATTAAAGGCTGTAAGATGATGATCGATGTTATAAAGACCCAGTTTCCTCAGCTGAAGAA AGTGATTCAGTTTGTTTGTGGAAATGGCCTTGTCTGTGAGACTGTGGAAGAGGCAAGACATATTGCATTT GGTGGACCTGAAAGACGAAAGGCAGTAGCACTTGATGGAACACTGTTTTTGAAATCTGGAGTGATCTCAG GAGGGTCAAGTGACTTAAAGCACAAAGCTCTGTGTTGGGATGAGAAGGAGTTACACAGTCTAAGAGATAA AAGAAACCAACTTGTCCAAGAGCTAAAGGAGTTAATGAAGACGCTCCGCAAGGAAACAGATCTGAAACAA ATACAGGCATTAGTACAAGGAACCAATACACGACTCAAATATTCACAAAATGAACTAGAGATGATTAAAA AGAAGCACCTTGCTACATTTTACCGGGAACAATCTCAGCTACAAAGTGAATTACTGAATATTGATTCTCA GTGTACAATGTTGAGCGAAGGAATCAACAAACAGCAACAAAAGATTGAAGAATTTCAAGATAAGATAGAC AAGGTAGAAGATGACATATTCCAAGACTTCTGTGAAGAAATTGGTGTGGAAAATATCCGTGAATTTGAGA ACAAACATGTTAAACAGCAGCAAGAAAATGATCAAAAAAGATTAGAGTTTGAAAAACAAAAAACTCGGCT CAATATTCAGCTTGAATATAGTCGAAATCAACTTAAGAAGAAACTGAATAAGATCGACACATTAAAAACA ACAATCCAGAAGGGCAGAGAAGACATTGATAACCTAAAGAAGACCGAAGAAGAGTGTCTGAAAATTGTTG AAGAACTCATGGTGAAACAAGAACAAGTTAAGGAAGTGCTTGCCACACAGAGTTCCAACATTGAGAAAAT TCATATTCAAATTGAAGAGGAACGCAAGAAGTTTTTGGCTGTTGATAGGGAAGTTGGAAAATTACAGAAG GAAGTTGTAATCATTCAGGGTTCTTTGGAACAGAAACAACTAGAGAAGCATAACTTGCTCCTGGACTGCA AAGTTCAAGACATTGACATAAGTCTCATGCTGGGGTCATTGGAGGACATCATTGAAGTGGAGCTAACTGA AACAGAAAGTACCCAGGCAACGGCTGATATCTATGAAAAAGAAGCATCCATCCAAATAGACTACAGCCCT CTAAGGGAGGATTGGAAGGCTCTACAATCAGATAAGGAGGTGGAGGCCCACCTCACACTCCTTCTACAGC AAGTAGCATCCCAAGAAAATACTCTACTGAAGACTGCAGCTCCAAACCTGCGAGCACAGGAGAACTTAAA GGCTGTCAGAGACAAATTTCAAGAATCTGCAGATGCTTTTGAGGCCAGCAGAAAGGAAGCCAGAATATGT AGGCAAGAGTTTGAACAGGTGAAAAGACGGAGGTACGATGCTTTCAGTCAGTGTTTTGAGCACATCTCGG TCTCAATTGATCAAATCTACAAGAAGCTCTGCAGGAACAACAGTGCCCAGGCATTTCTTAGCCCAGAGAA CCCTGAAGAACCTTACTTAGATGGAATTAGCTATAATTGTGTGGCTCCAGGCAAACGGTTCATGCCTATG GACAACTTGTCAGGGGGAGAAAAGTGTGTGGCTGCTCTGGCTCTTCTGTTTGCTGTACACAGTTTTCGAC CTGCTCCATTCTTTGTATTAGATGAAGTAGATGCAGCCCTGGACAATACTAACATCGGCAAAGTCTCAGG TTACATCAAAGAGCAGAGTCAGGAACAATTTCAGATGATAATCATTTCCCTGAAAGAGGAGTTTTACTCC AGAGCTGATGCACTGATAGGCGTCTACCCAGAGCACGATGAATGCATGTTCAGCCATGTGTTGACTCTGG ACCTTTCCAAGTATCCAGACACTGAAGACCAAGAAGGCAGCAGGAGCCACCGGAAACCCAGAGCACGAGT ATCACAGTCCCCGCAGTCTCCCCAGTCTCGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001130498
Insert Size	3744 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001130498.1, NP_001123970.1
RefSeq Size	4026 bp
RefSeq ORF	3744 bp
Locus ID	300121

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