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Med20 (NM_001013178) Rat Untagged Clone
CAT＃: RN202486
Med20 (untagged ORF) - Rat mediator complex subunit 20 (Med20), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Trfp; Usp49 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN202486 representing NM_001013178
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTGACGTGTGTGTCCCAGATGCCTGTGGCTGAGGGCAAGAGTGTCCAGCAGACAGTGGAGCTCC TCACCAAGAAATTGGAGATGCTTGGAGCAGAGAAACAAGGAACATTTTGTGTGGACTGTGAAACTTACCA CACAGCTGCCTCCACCCTGGGCAGCCAAGGCCAGGCTGGGAAGCTGATGTACGTGATGCATAACTCTGAG TACCCACTGAGCTGCTTTGCCCTCTTTGAGAATGGCCCTTGCCTTATTGCTGACACCAACTTCGATGTGC TCATGGTGAAGCTCAAGGGCTTTTTCCAGAGTGCCAAGGCCAGCAAGATTGAGACCCGGGGTACTCGCTA CCAGTATTGTGACTTCCTGGTGAAGGTGGGCACGGTCACAATGGGGCCCAGTGCCCGTGGCATCTCTGTG GAGGTGGAGTATGGCCCTTGTGTGGTAGCTAGTGACTGCTGGAGCCTCCTGCTTGAATTCCTACAGAGTT TTCTAGGTAGCCACACACCAGGGGCGCCCACAGTGTTTGGGAACAGACATGATGCGGTCTACGGGCCAGC AGATACCATGGTACAGTACATGGAGCTCTTCAACAAGGTCCGGAAACAGCAGCAAGTGCCTGTGGCTGGG ATCCGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001013178 |
| Insert Size | 639 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001013178.2, NP_001013196.1 |
| RefSeq Size | 1964 bp |
| RefSeq ORF | 639 bp |
| Locus ID | 316209 |
| UniProt ID | Q5XIE9 |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR202486 | Med20 (Myc-DDK-tagged ORF) - Rat mediator complex subunit 20 (Med20), (10 ug) |
CNY 3,990.00 |
|
| RR202486L3 | Lenti ORF clone of Med20 (Myc-DDK-tagged ORF) - Rat mediator complex subunit 20 (Med20), (10 ug) |
CNY 6,080.00 |
|
| RR202486L4 | Lenti ORF clone of Med20 (mGFP-tagged ORF) - Rat mediator complex subunit 20 (Med20), (10 ug) |
CNY 6,650.00 |
