Abcc5 (NM_053924) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Abcc5a; Mrp5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202372 representing NM_053924 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGATATTGACATGGGAAAAGAATACATCATCCCCAGTCCCGGATACAGAAGCGTCAGGGACAGAA GCACTATCCCGGGACAACACGGCGATCGTGAAGAACCCAGGTTCCGGAGAACAAGATCATTGGAATGCCA AGATGCCCTAGAAACAGCAGCCCGAGTTGAGGGTCTTTCCCTGGACATCTCTGTGCATTCTCATCTCCAA ATTCTGGACGAGGAACATACGAAGGGGAAATACCACCATGGTTTAAGCGCCCTGAAGCCCTTCCGGACCA CCACCAAGCACCAGCACCCAGTGGACAATGCTGGCCTTTTCTCCTACATGACCTTTTCATGGCTCTCTCC TCTGGCCCAAGTGGTTCACAAGAAGGGGGAGCTGTTAATGGAGGATGTGTGGCCTTTGTCCAAGTATGAG TCTTCTGATGTGAACTGCAGAAGACTAGAGAGACTGTGGCAAGAAGAGCTGAATGAAGTCGGGCCAGACG CCGCCTCCCTGCGAAGGGTTGTGTGGATCTTTTGCCGCACCAGGCTCATCCTGTCCATCGTGTGCCTGAT GATCACGCAGTTGGCTGGCTTCAGTGGACCAGCCTTCGTGGTGAAGCACCTCTTGGAGTACACCCAGGCA ACAGAGTCTAACCTGCAGTACAGCTTGCTGTTGGTACTAGGCCTCCTCCTGACAGAGGTTGTACGCTCCT GGTCACTCGCACTGACTTGGGCATTGAATTATCGCACTGGTGTCCGGCTGCGGGGGGCTGTCCTGACCAT GGCATTCAAGAAGATCCTGAAGCTAAAGAACATTAAGGAAAAGTCCCTGGGGGAGCTTATCAATATCTGC TCCAACGATGGGCAGAGGATGTTTGAAGCAGCCGCTGTGGGCAGCTTGCTGGCCGGAGGACCCGTTGTCG CCATCTTGGGCATGATTTATAATGTAATCATTCTAGGACCAACGGGCTTCCTGGGATCGGCTGTCTTTAT CCTCTTTTATCCGGCAATGATGTTCGTGTCACGGCTAACCGCGTATTTCAGGAGAAAGTGTGTAGCCGCC ACAGACGACCGTGTCCAGAAGATGAATGAAGTTCTTACCTACATTAAATTTATTAAAATGTATGCCTGGG TCAAAGCGTTTTCTCAGTGTGTTCAAAAAATCCGAGAGGAGGAGCGTCGGATCCTGGAGAAGGCCGGGTA CTTTCAGAGCATCACCGTTGGAGTGGCTCCCATTGTCGTAGTGATCGCCAGCGTGGTGACGTTCTCCGTC CACATGACCCTGGGCTTCGACCTGACAGCGGCGCAGGCCTTCACAGTGGTGACTGTCTTCAATTCCATGA CTTTTGCTTTAAAAGTAACACCATTCTCAGTGAAGTCCCTCTCTGAAGCATCCGTTGCTGTTGACAGATT TAAGAGTTTGTTTCTAATGGAAGAGGTTCACATGATAAAGAACAAACCGGCCAGTCCTCACATCAAGATA GAGATGAAAAATGCCACCTTGGCATGGGACTCCTCCCACTCCAGTACACAGAGCTCGCCCAAGCTGACCC CCAAAGTGAAAAAAGACAAGAGGGCTCCCAAGGGCAAGAAAGAGAAGTCGAGGCAGCTGCAACATACTGA GCACCAGGCGGTGCTGGCAGAGCAGAAAGGACACCTCCTCCTGGACAGTGATGAGCGGCCCAGTCCGGAA GAGGAAGAAGGCAAGCAGATCCACGCAGGGAGCATGCGTCTGCAGAGGACACTCTACAACATTGACTTAG AGATTGAAGAGGGCAAACTGGTTGGAATCTGTGGCAGTGTGGGAAGTGGAAAAACCTCTCTGATTTCCGC CATTTTAGGCCAGATGACGCTTTTGGAGGGCAGCATTGCCGTCAGTGGGACCTTTGCTTACGTGGCCCAA CAGGCCTGGATTCTCAACGCCACGCTGAGAGACAACATTCTCTTTGGGAAGGAATTTGATGAAGAGAGAT ACAACTCAGTGCTGAACAGTTGCTGCCTGCGGCCTGACTTGGCCATTCTCCCCAACAGCGACCTGACTGA GATTGGAGAGCGAGGAGCCAACCTGAGTGGTGGGCAGCGCCAGCGAATCAGCCTTGCTCGAGCCCTGTAC AGTGACAGAAGCATCTATATCTTGGATGATCCCCTCAGTGCCTTAGATGCCCATGTGGGCAACCACATCT TCAACAGTGCTATCCGGAAGCGTCTCAAGTCTAAGACGGTCCTGTTTGTCACACACCAGTTACAGTACCT GGTCGATTGTGATGAGGTGATCTTCATGAAAGAAGGCTGTATCACAGAGAGAGGTACCCACGAGGAGCTG ATGAACTTAAATGGAGACTACGCTACCATTTTTAATAACCTGTTGCTGGGAGAGACACCCCCGGTCGAGA TTAATTCAAAAAAGGAAGCTAGTGGTTCACAAAAATCACAAGACAAGGGCCCTAAGCCAGGGTCAGTGAA GAAGGAGAAGGCAGTGAAGTCGGAGGAAGGGCAGCTCGTGCAGGTGGAGGAGAAAGGGCAGGGCTCCGTG CCTTGGTCAGTTTACTGGGTCTACATCCAGGCTGCAGGGGGTCCCTTGGCTTTCCTGGTCATCATGGTCC TCTTCATGCTCAATGTGGGCAGCACTGCCTTCAGCACCTGGTGGCTAAGCTACTGGATCAAGCAAGGAAG CGGGAACAGCACGGTGTTTGAAGGGAACAGAAGCTCTGTGAGTGACAGCATGAGGGACAACCCCTTCCTG CAGTACTATGCCAGCATCTACGCTCTCTCCATGGCAGTCATGCTGATCCTGAAGGCCATCCGAGGAGTTG TCTTTGTCAAGGGTACACTGAGAGCCTCCTCCCGGCTCCATGATGAGCTCTTCCGGAGGATCCTGAGAAG CCCCATGAAGTTTTTTGATACTACCCCAACAGGAAGGATTCTCAACAGGTTTTCCAAAGACATGGATGAA GTGGACGTGCGTCTGCCATTCCAGGCTGAGATGTTTATCCAGAATGTGATCCTGGTGTTCTTCTGTGTTG GAATGATCGCAGGGGTCTTCCCATGGTTCCTTGTGGCAGTGGGGCCTCTCCTCATCCTCTTCTCAGTTCT ACACATTGTCTCCAGGGTCCTGATTCGGGAGCTGAAGCGCTTGGACAATATCACGCAATCTCCTTTCCTC TCCCATATCACGTCCAGCATTCAGGGCCTCGCCACCATCCATGCCTATAACAAAAGGCAGGAGTTTCTAC ACAGGTATCAGGAGCTTCTGGATGACAACCAAGCCCCCTTCTTCTTGTTCACCTGTGCAATGAGGTGGCT GGCAGTGCGGCTGGACCTCATCAGCATCGCCCTGATCACCACCACGGGGCTCATGATTGTTCTCATGCAC GGGCAGATCCCTTCAGCGTACGCGGGCCTCGCCATCTCCTACGCTGTGCAGTTAACCGGACTCTTCCAGT TTACCGTTAGACTGGCGTCGGAGACAGAAGCACGGTTCACCTCAGTGGAGAGGATCAACCACTACATAAA GACTCTCTCTTTGGAAGCACCTGCCAGAATCAAGAACAAGGCTCCTCCCCATGACTGGCCCCAGGAGGGA GAAATAACCTTTGAGAATGCGGAAATGAGATACCGGGAAAATCTCCCTCTGGTCCTTAAGAAAGTGTCCT TCACCATCAAGCCCAAGGAAAAGATAGGCATTGTGGGACGAACAGGGTCAGGGAAGTCCTCTCTAGGGAT GGCCCTCTTCCGCCTGGTGGAGTTATCTGGAGGCTGCATCAAGATCGATGGAGTAAGAATCAGTGACATT GGCCTGGCCGACCTCCGAAGCAAACTGACCATCATCCCTCAGGAGCCAGTGCTGTTCAGCGGCACAGTCA GATCAAACCTGGACCCTTTCAACCAGTACACAGAAGAGCAGATCTGGGATGCCCTAGAGAGAACACACAT GAAGGAATGTATTGCCCAGCTACCTCTGAAACTGGAATCTGAAGTAATGGAGAACGGGGACAACTTCTCT GTTGGGGAACGGCAGTTGTTATGCATAGCAAGGGCCCTGCTACGTCACTGTAAGATTCTGATTTTAGATG AAGCTACAGCCGCTATGGACACAGAGACAGACTTACTGATCCAAGAGACCATCCGGGAAGCATTTGCTGA CTGCACCATGCTGACCATTGCCCATCGCCTGCACACAGTTCTGGGCTCTGATAGGATCATGGTGCTGGCC CAGGGACAGGTGGTGGAGTTTGACACCCCGTCGGTCCTTCTGTCCAACGACAGCTCAAGATTCTATGCCA TGTGTGCTGCTGCAGAGAACAAGGTGGCTGTCAAGGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053924
Insert Size	4311 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053924.1, NP_446376.1
RefSeq Size	5744 bp
RefSeq ORF	4311 bp
Locus ID	116721
UniProt ID	Q9QYM0
Gene Summary	ATP-binding cassette (ABC) multiple drug resistance protein that mediates efflux cGMP during nitric oxide-induced regulation of smooth muscle [RGD, Feb 2006]

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