Abcc10 (NM_001108201) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202266 representing NM_001108201 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGCTCCTGGCCCAGCTTTGTGGCACGGATGCGGCGCGGCCACTCCCGCTCTGGGAAGGGGACA CCACGGGCCACTGCTTCACCCAGCTGGTGCTCAGCGTCCTTCCACACGCTCTCCTCGCCGTCCTCAGCGC CTGCCACTTGGGCACCCCAAGGACTAACAATATCATCCAACCTTTCAATCCTGGCTGGCGCCTCCGACTC ACAGCTTCCTTCCTCCTCTCCGTCTTCCCGCTGCTAGACCTCCTTCCGGTTGTTTTACCTCCGGTGTCAC ACCCAGGGCCCCTATGGCTAGAAGTGCTGGCAGGGTGTGTGACTGCTGTGGCCTGGTTCACCCACAGCCT GGCCCTGTGGGCATTGGTTCATTCCCCTCATGGCCATTCTCGTGGACCCTTGGCCTTGGCTCTGGCAGCC TTCTTGCCAACCCCAGCCCTGGTACTGACCCTGCTGTGGCATTGCCAGCGAGGTACATTTCTGCCCCCGC TTCTCCCAGGACCTCTGGGCCGTGTGTGTCTTCTCATCCTGCAGTTGGCAGCCGTCTTGGCCTATGGGCT GGGCTGGGCAGCCCCTGGGGGACCTCGAGAGCCCTGGACTCACGATCCCTTCCTGTCCCCTGAGAGTCAA GAAAGAGAGGTAGCTGAAGACGGAGAGAGTTGGCTGTCACGCTTTTCCTATGCCTGGTTGGCACCTCTGC TTGCTCGTGGAGTCCGAGGAGAGCTCCGGCAGCCCCAGGACACTTGCCGCCTCCCCCGCAGACTGCACCC TGCCTACCTGGCCCGAGCCTTCCAAGCGCACTGGAAGGAGGGGGCCCAACTGTGGAGGGCCCTGTATGGG GCCTTTGGATGTTGCTACATAGCCCTTGGACTGTTGAAGATGGTGGGGACCATGCTGGGCTTCTCTGGGC CTTTACTGCTCTCCCTACTGGTGGGCTTCCTGGAGGAAGGGCAAGAGCCTCTAAGCCACGGCCTGCTCTA CGTCCTGGGGCTGGCCAGTGGGTCTGTGATAAGCGCCGTACTACAGAACCAGTATGGGTATGAGGTGCGT AAAGTGACACTTCAGGCACGGGTGGCTGTGCTGAGCATCCTTTACCGCAAAACCTTGAAGCTTGGGCCTA GCCGCCCTCCCACTGGGGAGGTCCTGAACCTACTAGGCACCGACTCCGAGAGGCTGCTTAACTTTGCTGG CAGCTTCCATGAGGCCTGGGGCCTGCCTCTGCAACTGGCCATCACTCTCTACCTGCTGTACGAGCAGGTG GGCGTGGCCTTCCTGGCTGGGTTAGTCTTGGCACTGCTCCTGGTGCCTGTCAACAAAGTGATCGCCACCC GAATCATGGCCAACAACCAGGAGATGCTCCGGCACAAGGATGCACGGGTTAAGCTCATGACAGAGCTGCT GAGCGGCATTCGAGTCCTCAAGTTCTTCAGGTGGGAGCAGGCTCTGGGGGACCGGGTGAAGGCTTGCCGG ACCCAAGAGCTGGGACGGCTCCGCGTCATCAAATACCTGGATGCAGCCTGCGTGTACCTCTGGGCTGCTC TGCCTGTAGTCATCTGCATCGTCATCTTCATCACCTACGTCCTCCTGGGGCACCAGCTCACTGCCACCAA GGTGTTTACAGCACTGGCACTTGTGCACATGCTCATTCTTCCTCTCAACAACTTCCCCTGGGTGATCAAT GGTCTCTTGGAATCCAAAGTATCCCTGGACCGGATCCAGCGTTTCCTCGACCTTCCAAGCTATAGCCCTG AGGCCTACTACAGTCCTGATCCCCCCACAGAGCCATCCACAGTACTGGAGCTGCATGAAGCCCTGTTCTC CTGGGACCCAACTGGAACAAGCCAGAAGACCTTCATCAGTCACCTCCAAGTGAAAAAGGGCATGCTGGTG GGCATCGTGGGAAAGGTCGGCTGTGGGAAGAGTTCACTGCTGGCTGCCATCACTGGGGAGCTCCACAGGC TGTGTGGGTGGGTGGCAGTGTCAGACCTGTCCAAAGGCTTTGGACTGGCCACTCAGGAGCCCTGGATCCA GTGTGCCACCATCAGAGACAACGTCCTGTTTGGGAAGACATTTGATGCCCAGCTGTACAGGGAGGTGCTG GAGGCCTGCGCCCTCAATGATGATCTCAGCATCCTGCCTGCTGGAGACCAGACAGAGGTTGGAGAGAAGG GTGTGACCCTCAGCGGGGGACAGAGGGCCCGGATTGCCCTTGCCCGAGCTGTCTACCAGGAGAAAACACT CTACCTCCTTGATGACCCTCTGGCTGCTGTGGATGCAGATGTGGCCAACCACCTGTTGCACAGGTGCATC CTGGGAGTGCTGGGCCACACCACCCGGCTGCTGTGCACCCATCGCACCGAGTACCTGGAGAGGGCTGACC TGGTGCTGTTGATGGAGGCTGGGCGCCTGGTCCGAGCAGGGCCTCCCTCAGAGATTCTGCCATTGGTGCA AGCTGCCCCCACAGCCTTGGCTGACAAGGAACAAGTGACTGACTCAGGTCAGTCCCCGTCTGTACACAAC CTAGAGAAAACCACAGAGGAGCTGGACGTGGCGCAGAACACATCCGGTCGCCTCGTGCAGGAGGAGAGCA AAAGCGAGGGCGCCGTGGCCCTGCACGTGTACCGAGCATACTGGAGGGCCATGGGCAGTGGCCTGGCCAC CGCCATCCTCATCTCCCTGCTCCTCATGCAAGCCACGCGCAACGGTGCTGATTGGTGGCTCGCTCACTGG CTCTCTCAGCTGAAGGCAGGCAGAAATAGCTCCGAGGAGCGTCCCGCTTCCTCCAGCCCACGCTCCATGG GATTCTTCTCCCCACGGCTGCTCCTCTTCTCCCCCGGAAACCTCTACATCCCACTGCACAAAGCTACTTC CAATGGCTCCTCAGACGTCCACTTCTACCTCACCGTGTACGCAACCATTGCTGGTGTCAATTCCCTCTGC ACCCTGCTCCGGGCTGTGCTCTTTGCAGCAGGTGCCCTTCAAGCAGCTGCTAGCCTACACCACCGTCTAC TGCACCGACTGCTCATGGCACCAGTGACTTTCTATGACTCCACACCTTCCGGCCGGGTCTTGAACCGCTT CTCCTCAGACGTCGCCTGTGTAGACGACAGCCTGCCCTTCCTCCTCAACATCCTGCTGGCCAATTCTGTA GGGCTGCTGGGCCTCCTCGCTGTCCTGGGTTCTGGTCTGCCCTGGCTGCTGCTCCTGCTGCCGCCTCTGA GCTTCATCTACTACGGCGTGCAGCGCCGCTACAGGGCTTCCTTCCGGGAGCTGCGGCGTCTGGGCAGCCT CACCTTGTCTCCGCTCTACTCCCACCTGGCTGACACCCTGGCAGGCCTCCCTGTGCTCCGGGCTGCAGGG GCCACTTACAGGTTTGAGGAGGAGAACCAGCGACTCTTGGAGCTGAACCAGAGGTGCCAGTTTGCTTCCT ATGCCACGATGCAGTGGTTGGACATTCGGCTGCAGCTCATGGGGGCAGCGGTGGTCAGCGCCATTGCGGG CATTGCCCTGGTGCAGCACCAGCAGGGCCTTGCCAACCCAGGGCTGGTGGGCCTCGTGCTTTCTTATGCA CTGTCTCTGACGGGCCTGCTCTCCGGCCTGGTGAGCAGCTTCACACAGACGGAAGCCATGATGGTGAGCG TTGAACGACTGGAGGAATATTCCTGTGACATTCCCCAGGAGCCCCATGGCCAGCCACCACAGTCACCCCA CCAGCGCGTCAGCTGGCTGACCCAGGGAAGTGTGGAGTTCCAGGATGTGGTGCTGGTGTATCGGCCAGGG CTGCCAAATGCCCTAGACGGGGTGACCTTTCGCGTGGAGCCTGGCGAGAAGCTGGGCATTGTGGGCCGCA CAGGCTCCGGCAAGTCTTCCCTGTTTCTGGTGCTCTTCCGGCTGCTGGAGCCCAGTGCAGGGCAAGTGCT CCTGGACGGTGTGGACACCAGCCAGCTGGAGCTGGCTGAGCTCAGATCCCAGCTGGCTGTCATCCCCCAG GAGCCCTTCTTGTTCAGTGGGACCGTTCGGGAGAACCTGGACCCCCAGGGCCTACATGAGGACAGGGCCC TGTGGCAAGCCCTAGAACAGTGCCACCTGAGTGAGGTGGCCATGGCCATAGGAGGCCTGGATGGGGAGCT GGGCGAAAGGGGCAGGGACTTGTCCCTAGGGCAGAGACAGCTGCTATGCCTGGCAAGGGCTCTTCTCACA GATGCTAAGATCTTGTGCATTGACGAGGCCACGGCAAGTGTGGACCAGAAGACAGACCAGCTGCTCCAGC AAACCATCTGCAGACGCTTTGCCAACAAGACAGTGCTGACCATTGCCCACAGGCTCAACACGATCCTAAA CTCTGACCGGGTGCTGGTGCTCCAAGCCGGGAGGGTGGTGGAACTGGACTCCCCCTCAGCCCTGCGCAAC CAGCCCCATTCCCTGTTTAGGCAGCTCCTGCAGAGCAGCCAGCAGGGGGCCCACTCCCTTCCTGCCGCGT GCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108201
Insert Size	4485 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108201.1, NP_001101671.1
RefSeq Size	4859 bp
RefSeq ORF	4485 bp
Locus ID	316231

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