Acaca (NM_022193) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Acac; ACC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202137 representing NM_022193 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAACCATCTCCGTTGGCCAAAACCCTGGAGCTGAACCAGCACTCCCGATTCATAATTGGGTCCG TGTCTGAAGACAACTCAGAAGATGAGATCAGTAACCTGGTAAAGCTGGACCTAGAGGAGAAGGAGGGCTC CCTGTCACCAGCCTCTGTCAGCTCAGATACACTTTCTGATTTGGGAATCTCTGCCTTACAGGATGGTTTG GCCTTTCACATGAGGTCCAGCATGTCCGGCTTGCACCTAGTAAAACAAGGTCGAGACAGAAAGAAAATAG ACTCTCAACGAGATTTCACTGTGGCTTCGCCAGCAGAATTTGTTACTCGTTTTGGGGGAAATAAAGTGAT TGAGAAGGTTCTTATCGCCAACAATGGTATTGCAGCAGTGAAATGCATGCGATCTATCCGGCGGTGGTCT TATGAAATGTTCCGCAATGAACGTGCCATCCGGTTTGTTGTCATGGTTACACCCGAAGACCTTAAAGCCA ATGCAGAATACATTAAGATGGCGGATCACTATGTTCCAGTGCCTGGAGGAGCAAACAACAACAATTATGC AAATGTGGAATTGATTCTTGATATTGCGAAAAGGATACCTGTACAGGCAGTGTGGGCTGGCTGGGGTCAT GCCTCCGAGAACCCCAAGCTCCCGGAGCTACTCTTAAAAAATGGCATTGCTTTCATGGGCCCTCCGAGTC AGGCCATGTGGGCGTTGGGGGATAAGATTGCATCGTCTATTGTGGCTCAAACTGCAGGTATCCCCACTCT TCCCTGGAGTGGCAGTGGTCTTCGAGTGGATTGGCAAGAAAATGATTTTTCGAAACGCATCTTAAATGTT CCACAGGATCTGTATGAGAAAGGCTATGTGAAGGATGTGGATGATGGACTGAAGGCAGCCGAGGAGGTTG GCTATCCAGTGATGATCAAGGCCTCAGAGGGAGGAGGAGGGAAGGGAATCAGAAAAGTTAACAATGCAGA TGACTTCCCTAACCTCTTCAGACAGGTTCAAGCTGAAGTCCCTGGATCACCTATATTTGTAATGAGATTA GCAAAACAGTCTCGTCATCTGGAGGTCCAGATTCTGGCGGATCAGTATGGCAATGCAATTTCTTTGTTTG GTCGTGACTGCTCTGTACAACGCAGGCATCAGAAGATCATTGAAGAAGCTCCTGCTGCTATTGCTACCCC AGCAGTATTTGAACACATGGAACAGTGTGCTGTAAAACTTGCCAAAATGGTTGGTTATGTGAGCGCTGGG ACTGTGGAATACTTGTACAGCCAGGACGGAAGCTTCTACTTCTTGGAACTGAACCCTCGGCTACAGGTTG AACATCCTTGTACAGAGATGGTGGCTGATGTCAATCTTCCTGCAGCACAGCTCCAGATTGCCATGGGGAT CCCTCTATTTAGGATCAAGGATATTCGTATGATGTATGGGGTATCTCCTTGGGGTGATGCTCCCATTGAT TTTGAAAATTCTGCTCATGTTCCTTGCCCAAGGGGCCATGTGATTGCTGCTCGGATCACCAGTGAAAACC CAGATGAGGGGTTTAAGCCCAGCTCTGGAACAGTTCAGGAACTTAATTTTCGCAGCAATAAGAATGTTTG GGGTTATTTCAGTGTTGCTGCTGCTGGGGGACTTCATGAATTTGCTGATTCTCAGTTCGGTCACTGCTTT TCCTGGGGAGAAAACAGAGAAGAAGCAATTTCAAATATGGTGGTGGCATTGAAGGAGCTGTCTATTCGGG GTGACTTTCGAACTACAGTAGAATACCTCATCAAGCTGCTGGAGACAGAAAGCTTTCAGTTGAACAGAAT CGACACTGGCTGGCTGGACAGACTGATTGCAGAGAAAGTGCAGGCAGAGCGTCCTGACACCATGTTGGGA GTTGTGTGTGGGGCTCTTCATGTGGCAGATGTGAACCTGAGAAATAGCATCTCTAACTTCCTTCACTCCT TAGAGAGGGGTCAAGTCCTTCCTGCTCACACACTTCTGAACACAGTAGATGTTGAACTTATCTATGAAGG AATCAAATATGTACTTAAGGTGACTCGGCAGTCTCCCAACTCCTACGTGGTGATAATGAACGGCTCGTGT GTGGAAGTGGACGTGCACCGGCTGAGTGATGGTGGACTGCTCTTGTCCTACGATGGCAGCAGTTACACCA CATACATGAAGGAGGAGGTAGACAGATACCGAATCACAATTGGCAATAAAACCTGTGTGTTTGAGAAGGA AAATGACCCGTCTGTAATGCGCTCCCCGTCTGCTGGGAAGTTAATCCAGTATATTGTGGAAGATGGAGGC CATGTGTTTGCTGGCCAGTGCTATGCAGAGATTGAGGTAATGAAGATGGTAATGACTTTGACAGCTGTAG AATCTGGCTGCATCCATTATGTCAAGCGACCTGGAGCAGCACTTGACCCAGGCTGTGTGATAGCCAAAAT GCAGCTGGACAATCCCAGTAAAGTTCAACAGGCTGAGCTTCACACGGGCAGTCTGCCCCAGATCCAGAGC ACAGCTCTCCGAGGCGAAAAGCTCCATCGAGTTTTCCACTATGTCCTGGATAACCTGGTCAATGTGATGA ATGGATACTGCCTTCCAGACCCTTTCTTCAGCAGCAAGGTAAAGGACTGGGTAGAACGGTTAATGAAGAC TCTGAGAGACCCCTCCCTGCCTCTTCTAGAATTGCAGGATATCATGACCAGTGTCTCTGGCCGGATCCCC CTCAACGTGGAGAAGTCTATTAAGAAGGAAATGGCTCAGTATGCTAGCAACATCACATCGGTCCTGTGTC AGTTTCCCAGCCAGCAGATTGCCAACATCCTAGATAGTCATGCAGCTACACTGAACCGGAAATCGGAGCG GGAAGTCTTCTTCATGAACACCCAGAGCATTGTCCAGCTGGTGCAGAGGTACCGAAGTGGCATCCGTGGC CACATGAAGGCTGTGGTGATGGATCTGCTCCGGCAGTACCTGCGGGTGGAGACACAGTTTCAGAATGGCC ACTACGACAAATGTGTATTCGCCCTTCGGGAAGAGAACAAAAGTGACATGAACACCGTACTGAACTACAT CTTCTCCCACGCCCAGGTCACCAAGAAGAATCTCCTGGTGACAATGCTTATTGATCAGTTGTGTGGCCGG GACCCTACACTTACTGATGAGCTGCTAAACATCCTCACAGAGCTAACTCAGCTCAGCAAAACCACCAACG CCAAAGTGGCACTGCGGGCTCGCCAGGTTCTTATTGCCTCCCATTTGCCATCGTACGACGTTCGCCATAA CCAAGTAGAGTCCATCTTCTTATCAGCCATCGACATGTATGGACACCAGTTTTGCATTGAGAACCTGCAG AAACTCATCCTATCAGAAACATCTATTTTCGATGTCCTCCCAAACTTTTTTTACCACAGCAACCAGGTGG TGAGGATGGCGGCTCTGGAGGTATATGTTCGAAGAGCTTATATCGCCTATGAGCTCAACAGTGTACAGCA TCGCCAGCTTAAGGACAACACCTGTGTGGTAGAATTTCAGTTCATGCTGCCCACATCTCATCCAAACAGA GGGAACATCCCCACGCTAAACAGAATGTCCTTTGCCTCCAACCTCAACCACTACGGCATGACTCATGTAG CTAGTGTCAGCGATGTTCTGTTGGACAACGCCTTCACACCACCTTGTCAACGCATGGGCGGGATGGTCTC TTTCCGGACCTTTGAAGATTTCGTCAGGATCTTTGATGAAGTAATGGGCTGCTTCTGCGACTCCCCACCC CAAAGCCCCACATTCCCAGAGTCCGGTCACACTTCACTCTATGATGAGGACAAGGTCCCCAGGGACGAAC CAATACATATTCTGAATGTGGCTATCAAGACTGATGGCGATATTGAGGATGACAGGCTTGCAGCTATGTT CAGAGAGTTCACCCAACAGAATAAAGCTACTCTGGTTGAGCATGGGATCCGGCGACTTACGTTCCTAGTT GCACAAAAGGATTTCAGAAAACAAGTCAACTGTGAGGTGGATCAGAGATTTCATAGAGAATTCCCCAAAT TTTTCACATTCCGAGCAAGGGATAAGTTTGAGGAGGACCGCATTTATCGACATCTGGAGCCTGCTCTGGC TTTCCAGTTAGAGCTGAACCGGATGAGAAATTTTGACCTTACTGCCATTCCATGCGCTAATCACAAGATG CACCTGTACCTTGGGGCTGCTAAGGTGGAAGTAGGCACAGAAGTGACTGACTACAGGTTCTTTGTTCGTG CGATCATCAGGCACTCTGATCTGGTCACGAAGGAAGCTTCTTTTGAATATCTACAAAATGAAGGAGAGCG ACTGCTCCTGGAAGCCATGGATGAATTGGAAGTTGCTTTCAATAACACAAATGTTCGCACAGACTGTAAC CATATATTCCTCAACTTTGTGCCCACAGTCATCATGGACCCATCAAAGATTGAAGAATCTGTGCGGAGCA TGGTAATGCGCTATGGAAGCCGGCTGTGGAAATTGCGGGTCCTCCAGGCAGAACTGAAAATCAACATTCG CCTGACAACAACTGGAAAAGCGATTCCCATCCGCCTCTTCCTGACAAACGAGTCTGGCTACTACTTGGAC ATCAGCCTGTATAAGGAAGTGACTGACTCCAGGACAGCACAGATCATGTTTCAGGCGTATGGAGACAAGC AGGGACCACTGCATGGAATGTTAATCAATACTCCGTATGTGACCAAAGACCTTCTTCAATCAAAGAGGTT TCAGGCACAGTCCTTGGGAACAACGTATATATATGATATCCCAGAGATGTTTCGGCAGTCGCTCATCAAG CTCTGGGAGTCCATGTCCACTCAAGCATTTCTTCCTTCACCCCCTTTGCCTTCCGACATACTGACGTATA CTGAACTGGTGTTGGATGATCAAGGCCAGCTTGTCCATATGAACAGACTTCCAGGAGGAAACGAGATTGG CATGGTAGCCTGGAAAATGAGCCTTAAAAGCCCTGAATATCCAGATGGCCGAGATGTCATTGTCATCGGC AATGACATTACATATCGGATTGGCTCCTTTGGGCCTCAGGAAGATTTGCTGTTTCTCAGAGCTTCTGAAC TTGCCAGAGCAGAAGGCATCCCACGCATCTACGTAGCAGCCAACAGTGGAGCTAGAATTGGACTGGCAGA AGAAATCCGTCATATGTTCCACGTGGCCTGGGTAGACTCTGAGGATCCTTACAAGGGATACAAGTATTTA TATCTGACACCCCAGGATTATAAAAGAGTGAGTGCTCTCAATTCTGTCCACTGTGAACATGTGGAAGATG AAGGAGAATCCAGGTACAAGATAACAGACATTATCGGGAAAGAAGAAGGACTTGGAGCAGAGAACCTTCG GGGTTCTGGAATGATTGCTGGGGAATCCTCATTGGCTTATGATGAGATCATCACCATCAGCCTGGTTACA TGCCGGGCCATTGGTATTGGGGCTTACCTTGTCCGGCTGGGACAAAGAACCATCCAGGTTGAGAACTCTC ACTTAATTCTAACAGGAGCCGGTGCCCTCAACAAAGTCCTTGGTCGGGAAGTATACACCTCCAACAATCA GCTTGGGGGCATCCAGATAATGCACAACAACGGAGTTACCCATTGCACTGTTTGTGATGACTTTGAGGGA GTGTTCACAGTCTTACACTGGCTGTCATACATGCCTAAGAACGTGCACAGTTCAGTTCCTCTCCTGAATT CCAAGGATCCTATAGATAGAATCATCGAGTTTGTTCCCACAAAGGCCCCGTATGATCCTCGGTGGATGCT GGCAGGCCGTCCTCACCCAACCCAGAAAGGCCAATGGTTGAGTGGATTTTTTGATTATGGCTCTTTCTCA GAAATCATGCAGCCCTGGGCGCAGACCGTGGTAGTTGGCAGAGCCAGGTTGGGGGGAATACCTGTGGGAG TAGTTGCTGTAGAAACCCGAACCGTGGAGCTCAGTGTACCAGCTGATCCTGCAAACCTGGATTCTGAAGC CAAGATAATCCAGCAGGCCGGCCAAGTTTGGTTTCCAGACTCTGCATTTAAGACCTATCAAGCTATCAAG GACTTTAACCGTGAAGGGCTACCTCTAATGGTCTTTGCCAACTGGAGAGGCTTCTCTGGTGGGATGAAAG ATATGTATGACCAGGTGCTCAAGTTTGGTGCTTATATTGTGGATGGCTTGCGGGAATGTTCCCAGCCTGT GATGGTCTACATTCCCCCACAGGCTGAGCTTCGGGGTGGTTCTTGGGTTGTGATCGACCCAACCATCAAT CCTCGGCACATGGAGATGTATGCTGACCGGGAAAGCAGGGGATCCGTTCTGGAACCAGAAGGGACAGTAG AAATCAAATTCCGCAAAAAGGATCTGGTGAAAACCATGCGTCGCGTAGACCCAGTCTACATCCGCTTGGC TGAGCGACTGGGCACCCCAGAGCTAAGCCCCACTGAGCGGAAGGAGCTGGAGAGCAAGTTGAAGGAGCGG GAGGAGTTCCTAATTCCCATTTACCATCAGGTAGCTGTGCAGTTTGCTGACTTGCACGACACCCCAGGCC GGATGCAGGAGAAGGGTGTCATTAATGATATCTTAGATTGGAAAACATCCCGCACCTTCTTCTACTGGCG ACTGAGGCGTCTCCTGCTGGAAGACCTGGTCAAGAAGAAAATCCACAGTGCCAACCCTGAGCTGACCGAT GGCCAGATCCAGGCCATGTTGAGACGCTGGTTTGTGGAAGTGGAAGGCACAGTGAAGGCTTACGTCTGGG ACAATAATAAGGATCTGGTGGAATGGCTGGAGAAGCAGCTGACAGAGGAAGATGGTGTCCGCTCTGTGAT AGAGGAGAACATCAAATACATCAGCAGGGACTATGTCCTCAAGCAGATCCGCAGCTTGGTGCAGGCCAAT CCAGAAGTTGCCATGGACTCCATCGTCCACATGACCCAGCACATCTCCCCCACTCAGCGAGCAGAGGTTG TAAGGATCCTTTCCACTATGGACTCCCCTTCTACGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022193
Insert Size	7038 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022193.1, NP_071529.1
RefSeq Size	7038 bp
RefSeq ORF	7038 bp
Locus ID	60581
UniProt ID	P11497
Gene Summary	catalyzes the rate-limiting carboxylation of acetyl CoA to malonyl CoA during long-chain fatty acid synthesis [RGD, Feb 2006]

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